Alzheimer’s disease is an age-related neurodegenerative disorder and is seen in an estimated 35 million people worldwide  . It is estimated that in 30 years, the United States will see 15 - 20 million total cases of some cognitive disability  . Although this disorder is usually recognized with old age, there are uncommon cases seen in adults under the age of 65. These cases are called early-onset Alzheimer’s disease (EOAD), this group represents about 10% of all Alzheimer’s cases  .
Differences clearly exist between the two groups. In late-onset Alzheimer’s disease (LOAD), adults over the age of 65 show the characteristic decline in memory function and some mood swings. This then escalates over time to the patient being unable to properly control their own body. Unfortunately, in EOAD an estimated 30% of the patients will experience aphasia, apraxia and agnosia before memory disturbances  . It has also been reported that EOAD patients have a more rapid clinical decline and shorter survival period after diagnosis than LOAD patients  . Early-onset AD patients also show greater impairment in attention, language, visuo-spatial and executive functions. It should be noted that in neuroimaging studies, these same patients demonstrate more cortical atrophy, hypoperfusion, and hypometabolism  . These issues mostly occur in the parietal and lateral temporal cortices of the EOAD patient, whereas LOAD patients demonstrate these issues in the medial temporal cortices  .
When the hallmarks of Alzheimer’s disease are viewed: Amyloid Beta (Aβ) plaque and tau tangles, the EOAD and LOAD patients differ still. In the EOAD patients lower cerebrospinal fluid levels of Aβ plaque and very high total tau levels were displayed. These results were found with brain imaging techniques  . It should be noted that in a post-mortem study, it was found that Aβ burden for EOAD patients was exceedingly high like the tau  . Due to the previously mentioned differences in symptomology and presentation of the disease state, this paper will look to see if there is a difference in metabolic dysfunctions in EOAD patients as compared to LOAD patients.
2. Literature Review
2.1. Genetic Basis in Early Onset and Late Onset Alzheimer’s
It is currently believed that less than one percent of EOAD cases are caused by an autosomal dominant mutation. The genes that account for these familial cases are PSEN1, PSEN2, and APP (6). The familial cases account for a very small proportion of the early onset patients. More commonly seen is the APOE ε4 allele in sporadic AD cases. Flier stated, 50% - 60% of individuals with AD are ε4 carriers  . Those that are homozygous for the allele may develop EOAD up to 10 years earlier than individuals without the allele.
APOE in the brain is mostly produced by astrocytes and microglia. It functions as a ligand receptor-mediated endocytosis of lipoprotein particles  . There are three major isoforms of APOE: APOE2, APOE3, and APOE4. Kim indicated there is a strong association between the APOE gene and the presence of Aβ within the brain  . It’s believed that APOE may be an Aβ-binding protein that induces the conformational β-sheet change seen in Aβ plaque, especially the APOE ε4 allele. The same article also mentions the possibility of the ε4 allele being linked to metabolic alterations within the brain. The ε4 allele has been linked to increased levels of LDL and is a risk factor for cardiovascular disease  . Due to its link with atherosclerosis, this can cause decreased blood flow and altered metabolic properties to an inflicted individual’s brain. Kim discusses a positron study carried out on young and old non-dementia patients that were carriers for the APOE ε4 isoform  . The study found these individuals displayed similar regional patterns of hypometabolism seen in patients with AD of the same ages. Kim suggests that this could mean the APOE ε4 is linked to the brain metabolism decrease seen in AD  .
In a study carried out by Chartier-Harlin and colleagues, the APOE ε4 allele presence was compared between early-onset and late-onset Alzheimer’s patients and controls for the same age groups  . One curious concept found was that the frequency of the APOE ε4 allele in EOAD and LOAD cases was not significantly different. They also found that there was an inverse correlation between the age of onset and ε4 allele copy number for late-onset. This was not the case for early-onset cases. The more allele copies an individual in the LOAD category had, the earlier their onset occurred. Whereas the EOAD, it didn’t seem to matter the number of ε4 alleles they contained, their onset age was all relatively the same  .
2.2. Topographical and Cognitive Disparities
In a study carried out by Ishii and colleagues, the local gray matter concentration between early-onset AD and late-onset AD was compared, along with controls at the same age  . When data was viewed, they found significant atrophy in the bilateral medial temporal lobes. Ishii notes there were greater differences between the early-onset group and their controls than the late-onset group  . The EOAD had less gray matter density in the bilateral inferior parietal lobules, precuneus, perisylvian cortices, and basal forebrain region. The right inferior frontal gyrus lacked gray matter density when compared to the control for the early-onset age group. Early-onset demonstrated lower densities in the bilateral precuneus, left parietal cortices, right middle temporal gyrus, and left fusiform gyrus  .
In a study carried out by Frisoni and colleagues, they also compared the grey matter atrophy of EOAD and LOAD patients  . They tested a few other parameters such as neuropsychological tests and cortical mapping as well. For the neuropsychological tests, Frisoni and colleagues, showed that the early-onset patients did worse in tests that entailed visuospatial, frontal-executive, and learning concepts  . They also found the total gray matter atrophy in EOAD patients was greater than in LOAD, similar to the results found by Ishii. It should be noted, that atrophy for LOAD patients was higher in the left hippocampus  . The greatest loss for gray matter occurred in the occipital lobe for EOAD patients and in the hippocampus for LOAD patients. These results were found using volumetry, a volumetric analysis, and were later supported by the results this group found using cortical mapping techniques  . Figure 1 compiles Frisoni and Ishii’s work and compares the grey matter atrophy of early-onset patients and their control counterparts. Figure 2 compiles the same data and compares late-onset versus their aged control group.
In a study by Bird, postmortem levels of choline acetyltransferase activity in the brains of EOAD, LOAD, and age-matched controls were viewed  . Brains were removed, one half was used for immediate pathological evaluations, and the other half was frozen. The brains riddled with Alzheimer’s disease demonstrated extreme brain atrophy. Slides were made from cortex and stained with H and E, Holems, and periodic acid-Schiff stains. This was done to view density of plaques and tangles and to view the general distribution throughout the brain to assign disease severity. A biochemical analysis was then done on sections of the
Figure 1. The information shown above demonstrates the amount of atrophy seen in late onset Alzheimer’s patients compared to their same aged counterparts. Data was obtained from Frisoni and Ishii studies on grey matter atrophy   .
Figure 2. The information compiled compares early-onset Alzheimer’s patient’s brain volume to their same aged controls. Data for this diagram was used from Frisoni and Ishii studies   .
frozen brain from the frontal cortex, superior temporal gyrus, Ammon’s horn, putamen, and lateral cerebellar hemisphere  . Bird found that patients with early-onset AD showed significantly lower choline acetyl-transferase activity than age-matched controls in the frontal cortex, temporal cortex, hippocampus, and cerebellum. Whereas the late-onset AD patients demonstrated a significant decrease in choline acetyl-transferase levels in the hippocampus  . These neurochemical findings support results seen in Frisoni’s study  .
In a rat model study of AD, the metabolism in the rat brain was followed to determine the neurochemical outcome through the course of the disease. This study found that impaired neuronal mitochondria metabolism in the hippocampus, frontal cortex, frontal cingulate cortex, and retrosplenial cingulate cortex where a deceased amount of glutamate, GABA, and aspartate production occurred  . This reduced turnover suggests reduced TCA cycle flux in glutamatergic and GABAergic neurons in these rats. This supports the decreased glutamate levels in the cingulate cortex as seen in another study on AD patients  . They also showed decreased GABA and aspartate levels in postmortem brains which further support this, as discussed previously  .
2.3. Glucose Metabolism and Metabolic Differences
Studies that previously compared cerebrospinal fluid (CSF) levels of β-amyloid have been inconsistent in the past, they may either find no significant change, a slight decrease, or increase  . In Andreasen and colleagues study they analyzed the cerebrospinal fluid (CSF) levels of Aβ(1-42) and found a positive correlation between age and the CSF-β-amyloid level  . When compared within the Alzheimer’s patients, the CSF-Aβ level was significantly lower in EOAD than LOAD individuals. This study followed patients and tested their levels again at ten and twenty months. In all Alzheimer’s patients, there was no significant difference between CSF-Aβ levels at the baseline and ten month follow up. This study also compared the duration of dementia and their CSF levels, in which they found no significant correlation for this  .
It should be noted that in Chiaravalloti and colleague’s study, they found some different results in the cerebrospinal fluid samples  . In this study, Chiaravalloti and colleagues compared the levels of Aβ(1-42) and tau. It was discovered that EOAD and LOAD patients had comparable levels for Aβ(1-42) and tau, except for t-tau. The levels of t-tau were a little higher in the EOAD group  .
Cerebrospinal fluid is an important part of the brain, but blood flow is another measurable factor that needs to be considered. Within the brain, blood flow is what provides the needed oxygen and glucose used by the neurons to survive  . Without it high amounts of cell death or dysfunction are observed, as is seen in stroke patients. Patients with AD show a few changes in brain microcirculation like appearance of string vessels (collapsed membrane tubes), reduced capillary density, mitochondrial content decrease, loss of tight junctions, and blood brain barrier breakdown. Zlokovic states, individuals with AD have a decrease in GLUT1 expression at the Blood Brain Barrier (BBB) which leads to a shortage of necessary metabolic substances  .
In another study that followed vascular dysregulation, Iturria-Medina and colleagues, tested for over 230 biomarkers seen in LOAD patients and sorted them according to their abnormality  . They discovered Cortisol, Apolipoprotein A (helps with lipid metabolism), and interferon-γ-induced protein 10 (IP-10) demonstrated some of the highest abnormalities  . IP-10 alterations reflect peripheral inflammation, characteristic of neurodegenerative disorders, but it is also a modulator of angiogenesis. This information provides insight into the amount of vascular dysfunction seen in very early stages of Alzheimer’s.
Some research has shown that cognitive dysfunction can be linked to high-sugar/fat diet and a sedentary lifestyle  . In the study by Stranahan and colleagues, rats were fed a diet high in fat and simple sugars, water was replaced by high-fructose corn syrup  . This diet caused an increase in fasting blood glucose, serum cholesterol, and triglyceride levels. This diet impaired rat’s hippocampus-dependent learning abilities, synaptic plasticity, and the dendritic spine density  . These rats were tested eight months into the diet and were found to perform very poorly in the water maze compared to the control rats. They were more likely to take the longer route to the platform and took them in general longer to get to the hidden platform. Hippocampi were taken from sacrificed rats and the levels of BDNF, a neurotrophic factor regulated by dietary energy, were measured. Rats on the high sugar/fat diet had reduced BDNF in whole hippocampi homogenates, which it suggests synaptic loss  . This leads experts in the area to conclude that there may be a link between obesity and AD as well as a link between cognitive dysfunction and abnormal glucose homeostasis.
A study done on patients with Type 1 or 2 diabetes mellitus displayed significant memory impairment and attention deficits on cognitive testing compared to their controls  . Hyperglycemia leads to cognitive dysfunction in a number of possible ways. The excess glucose can activate the polyol pathway, which in hyperglycemia will increase consumption of NADPH  . The increased use of NADPH will deplete glutathione levels and lower the threshold for oxidative injury in neurons. Klein and Waxman found increased formation of glycosylation contributed to vascular damage  . Any of these can alter brain function via dysfunctional blood flow, vascular permeability, or end-organ injury.
Baker and colleagues carried out a similar study on 23 adults with no previous diagnosis of prediabetes or diabetes  . The adults had a glucose tolerance test done and fell into one of the two categories. None met the criteria for mild cognitive impairment. Patients were given an injection of Fludeoxyglucose F-18 (FDG), and the emission was scanned for 40 minutes after the injection. For 35 minutes of the scan, patients were provided a memory encoding task, a repeating list of 20 words  . Adults that had prediabetes or diabetes demonstrated reduced cerebral glucose metabolic rate in brain regions commonly afflicted by early Alzheimer’s disease. The Positron Emission Tomography (PET) scan in these adults showed a decrease in glucose metabolic rate in the posterior cingulate cortex, precuneus region, parietal cortices, temporal gyri, and prefrontal cortices  .
A positron emission tomography (PET) study on EOAD and LOAD patients viewed regional cerebral metabolic patterns  . To measure the cerebral glucose metabolic rate, CMRGlu, AD patients were given an IV injection of 2 [18F] fluoro-2-deoxy-D-glucose. On a PC-384 scanner, slices of the whole brain were taken and used to measure CMRGlu based on tissue activity  . It was found that the left temporal-parietal factor, which is involved in language cognition, processing, and comprehension, had the highest correlation to dementia severity in both EOAD and LOAD. When early-onset and late-onset were compared, this study found the right temporal-parietal factor to have significantly lower cerebral metabolism  .
In a study carried out by Kim and colleagues, a PET scan on participants compared the hypometabolic brain patterns in patients  . They found the LOAD group to have more hypometabolic issues in the right inferior temporal gyrus. Whereas the EOAD had hypometabolism in the frontal lobe, basal ganglia, thalamus, and the temporoparietal areas. This same pattern of extensive overall brain complications was seen in grey matter atrophy and neurochemical pattern changes as well  . This shows there is a greater reduction in cerebral glucose metabolism in early-onset AD patients compared to their late-onset counterparts. This study also compared glucose metabolism to see if it had an effect on dementia severity. They found hypometabolism of EOAD was mild at clinical dementia rating (CDR) 0.5, but became more widespread at higher CDR levels. When this was compared to LOAD patients, the metabolic reduction was mild until CDR 2  . Figure 3 demonstrates the disparities observed in glucose hypometabolism as observed by Kim et al., 2005 and Rabinovich et al., 2010.
These findings demonstrate differences associated with glucose metabolism. Taken together with the previous data, the results here point to early-onset Alzheimer’s disease as being an overall more wide-spread and faster paced disease than the late-onset version. This was demonstrated in the results obtained from previous studies that viewed the grey matter atrophy, the neurochemical changes, metabolic dysfunction, and glucose metabolism changes due to the metabolic dysfunction.
Alzheimer’s is believed to have many causes, a genetic basis, Amyloid Plaque and tau Tangles formation, or hypometabolism in the brain. All are thought to play a part in the more uncommon Early-Onset Alzheimer’s Disease. This review compared these parameters, and a few others to outline the differences between LOAD and EOAD, or see if there really is much of a difference.
The APOE ε4 allele is indicative that an individual is more likely to develop
Figure 3. In the above diagram, data from Kim and colleague’s study and Rabinovici and colleague’s study was used   . Both used different labeling techniques for PET scans to measure disparities in glucose hypometabolism. Scaling was based off of these images coloring patterns, with dark coloration being a 5 and the region being grey rates as a 0.
Alzheimer’s disease. This specific allele has been linked to conformational changes of the Aβ proteins, and causes them to clump together and diminish its role in neural growth and repair. The ε4 allele has been linked to metabolic alterations in the brain via increased low density lipoproteins (LDLs). This increase has been previously known to cause atherosclerosis, which affects blood flow, in this case to the brain. This same allele is not super indicative of when an individual will develop the hallmark signs of AD, but it is more common for individuals with this allele to have AD.
Differences in the atrophy and chemicals that occur in EOAD and LOAD brains supports the view that EOAD is a faster paced form of Alzheimer’s disease. Since EOAD patients have a difficult time with attention, language (including aphasia and apraxia), visuo-spatial, and executive functions, the locations of their greatest brain atrophy makes sense. It was shown that the greatest amount of atrophy for EOAD was in the bilateral inferior parietal lobes, occipital lobe, and basal forebrain. These same areas also demonstrated low levels of acetyl-transferase and GABA.
Some of the most trademark signs for the Late-Onset patients are their disappearing memory and mood swings. Their greatest location of atrophy and neurochemical dysfunctions was found in the hippocampus. This makes sense due to the hippocampus’s main role in emotion regulation, recalling long-term memories, and spatial navigation.
One of the newest theories is that AD may be linked to a decrease in sleep. Studies done in rodents support this idea since wakefulness resulted in the accumulation of Aβ. Likewise, NREM sleep supported glymphatic clearance of Aβ  . Just as likely, is the current theory involving hypometabolic dysfunction which has been related to high fat and sedentary lifestyles. Glucose is needed by the brain as the main source of energy. Without glucose the cells begin to die. When certain areas of the brain begin to experience microcirculation dysfunction, there is a lack in the needed glucose and metabolites. The Late-Onset individuals demonstrated hypometabolic dysfunction in the right inferior temporal gyrus, which is close to the hippocampus. The Early-Onset individuals demonstrated a more wide spread problem with hypometabolism. They found issues in the right temporal-parietal region, frontal lobe, thalamus, and basal ganglia. All of which are regions that have previously shown atrophy and neurochemical flaws in AD.
This review has demonstrated that Early-Onset Alzheimer’s, although a more uncommon form, is a more wide spread disease. When compared to the Late-Onset variant, EOAD demonstrates a broader array of complications that is more wide spread throughout the brain. This includes more damages due to atrophy across the entire brain, not just specific regions. The metabolic dysfunction and metabolism differences also demonstrated more wide spread metabolic dysfunction. Thus, this causes these patients to die much sooner after diagnosis than their Late-Onset counterparts. All of these patients demonstrate similar problems, just in different locations throughout the brain. More research needs to be done in the area of metabolic dysfunction for EOAD, and for all Alzheimer patients in general.
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