JWARP  Vol.10 No.3 , March 2018
Effect of Domestic Wastewater as Co-Substrate on Biological Stain Wastewater Treatment Using Fungal/Bacterial Consortia in Pilot Plant and Greenhouse Reuse
Abstract: In this study, a pilot wastewater treatment plant was used to evaluate the co-treatment of biological-staining residues and domestic wastewater under non-sterile conditions. A novel microbial consortia formed by Trametes versicolor, Trametes sp, Pleurotus ostreatus, Pseudomonas fluorescens, Pseudomonas azotoformans, Pseudomonas sp, Enterobacter xianfangensis and Bacillus subtillis was inoculated in an extended aeration type bio-reactor. The treatment units were operated during three consecutive cycles during a period of 147 h. After the last operating cycle, the concentrations of Chemical Oxygen Demand, Biochemical Oxygen Demand, Color Units, Total suspended solids, and the pH value were 1695 mg/L, 105 mg/L, 106 CU, <0.001 mg/L and 5.8 respectively. The initial values of wastewater were 6755 mg/L (COD), 2005 mg/L (BOD5), 1367 (CU), 566 mg/L (TSS) and 7.0 (pH) respectively. The reduction of pollutants load was related with the ratio of the two types of wastewater (3.5:0.5) combined to increase biodegradability, the concentration of fungi and bacteria used in the consortia (30 × 103 - 55 × 106 CUF/mL Total Fungi and 70 × 107 - 83 × 108 CFU/mL of Total Bacteria) and ligninolytic enzymes production, Laccase (13 - 96 U/L), MnP (9.8 - 39 U/L) and LiP (0.3 - 5.3 U/L). The post-treated effluent was used as irrigation water. Lolium perenne plants were watered during 60 days with post-treated effluent. The results of root weight showed that there are significant differences between the initial water and the effluent obtained after the operational cycles (p = 0.00470). The highest root weights (1 - 1.12 g) were found in plants irrigated with water obtained from the last treatment cycle.
Cite this paper: David, P. , Camilo, L. , Farid, R. , Felipe, M. , Stephanie, P. , Julio, R. , Janeth, M. , Carlos, S. , Ana, D. , Santiago, L. and Marina, P. (2018) Effect of Domestic Wastewater as Co-Substrate on Biological Stain Wastewater Treatment Using Fungal/Bacterial Consortia in Pilot Plant and Greenhouse Reuse. Journal of Water Resource and Protection, 10, 369-393. doi: 10.4236/jwarp.2018.103020.

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