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 Health  Vol.10 No.3 , March 2018
Xenobiotics-Induced Liver Damage Is Biochemically Abrogated by Treatment with Lipophilic Extracts of Moringa oleifera in Vivo
Abstract: Context Drug-induced hepatotoxicity represents a significant proportion of liver disease cases. Currently, there is no effective treatment. To date efforts to identify treatment regimen that can reverse progressive damage have not been successful. We have previously shown that extract from Moringa (M) oleifera possesses clinically relevant antidiabetic and electrolyte modulators. Objective The aim of the current studies is to create experimental model of xenobiotic induced liver damage and investigate if treatment with lipophilic extract of M. oleifera could biochemically reverse progressive liver damage. Materials and Method For two groups of healthy rats, 7 in each group received 200 mg of extract or vehicle twice daily for 14 days. Acute toxicity, hepatotoxicity and hematologic/endothelial toxicity were monitored. Then 30 rats weighing 130 - 200 g received repeated dose of acetaminophen (xenobiotics) (640 mg/kg) for 5 days. Hepatotoxicity was confirmed biochemically by an established protocol. Treatment with M. oleifera extract resulted in mean weight of 132.2 ± 5.05 compared to the control with 134.1 ± 5.08 (P > 0.8115) among the healthy rats. Their LDH levels were 170.7 ± 13.02 and 133.8 ± 7.17 (P > 0.0698) for controls group, while the mean serum (ALT) level was 12.4 ± 1.2 or 25.6 ± 5.644 (P < 0.01) for controls group. However, treatment of rats with hepatitis using lipophilic extract of M. oleifera resulted in 100% biochemical recovery from hepatitis compared to the control group (P < 0.0006). Conclusion This study strongly indicates that treatment with lipophilic extract of M. oleifera could effectively and biochemically abrogate xenobiotics induced liver damage in animal model.
Cite this paper: Omabe, M. , Omabe, K. , Igwe, D. , John, O. , Uchenna, S. and Elom, S. (2018) Xenobiotics-Induced Liver Damage Is Biochemically Abrogated by Treatment with Lipophilic Extracts of Moringa oleifera in Vivo. Health, 10, 313-325. doi: 10.4236/health.2018.103025.
References

[1]   Dechene, A., Sowa, J.P., Gieseler, R.K., et al. (2010) Acute Liver Failure Is Associated with Elevated Liver Stiffness and Hepatic Stellate Cell Activation. Hepatology, 52, 1008-1016.
https://doi.org/10.1002/hep.23754

[2]   O’Grady, J.G. (2005) Acute Liver Failure. Postgraduate Medical Journal, 81, 148-154.
https://doi.org/10.1136/pgmj.2004.026005

[3]   Pellicoro, A., Ramachandran, P., Iredale, J.P., et al. (2014) Liver Fibrosis and Repair: Immune Regulation of Wound Healing in a Solid Organ. Nature Reviews Immunology, 3, 181-194.
https://doi.org/10.1038/nri3623

[4]   Bourbonnais, E., Raymond, V.A., Ethier, C., et al. (2012) Liver Fibrosis Protects Mice from Acute Hepatocellular Injury. Gastroenterology, 142, 130-139.
https://doi.org/10.1053/j.gastro.2011.09.033

[5]   Protzer, U., Maini, M.K. and Knolle, P.A. (2012) Living in the Liver: Hepatic Infections. Nature Reviews Immunology, 12, 201-213.
https://doi.org/10.1038/nri3169

[6]   Omabe, M., Nwudele, C., Omabe, K.N., et al. (2014) Anion Gap Toxicity in Alloxan Induced Type 2 Diabetic Rats Treated with Antidiabetic Noncytotoxic Bioactive Compounds of Ethanolic Extract of Moringa oleifera. Journal of Toxicology, 2014, Article ID: 406242.
https://doi.org/10.1155/2014/406242

[7]   Omabe, M. and Kenneth, N.O. (2014) Insight in Inflammation and Cancer. The Atlas of Genetics and Cytogenetics in Oncology and Haematology, 18, 203-216.
https://doi.org/10.4267/2042/53491

[8]   Ghasi, S., Nwobodo, E. and Ofili, J.O. (2000) Hypocholesterolemic Effects of Crude Extract of Leaf of M. oleifera Lam in High-Fat Diet Fed Wistar Rats. Journal of Ethnopharmacology, 69, 21-25.
https://doi.org/10.1016/S0378-8741(99)00106-3

[9]   Lee, S.W., Kim, S.H., Min, S.O. and Kim, K.S. (2011) Ideal Experimental Rat Models for Liver Diseases. Korean Journal of Hepato-Biliary-Pancreatic Surgery, 15, 67-77.
https://doi.org/10.14701/kjhbps.2011.15.2.67

[10]   Workman, P., Aboagye, E.O., Balkwill, F., et al. (2010) Guidelines for the Welfare and Use of Animals in Cancer Research. British Journal of Cancer, 102, 1555-1577.
https://doi.org/10.1038/sj.bjc.6605642

[11]   Baravalia, Y. and Chanda, S. (2011) Protective Effect of Woodfordia fruticosa Flowers against Acetamino-phen-Induced Hepatic Toxicity in Rats. Pharmaceutical Biology, 49, 826-832.
https://doi.org/10.3109/13880209.2010.550057

[12]   Navarro, V.J. and Senior, J.R. (2006) Drug-Related Hepatotoxicity. The New England Journal of Medicine, 354, 731-739.
https://doi.org/10.1056/NEJMra052270

[13]   Kato, G.J., McGowan, V., Machado, R.F., et al. (2006) Lactate Dehydrogenase as a Biomarker of Hemolysis-Associated Nitric Oxide Resistance, Priapism, Leg Ulceration, Pulmonary Hypertension, and Death in Patients with Sickle Cell Disease. Blood, 107, 2279-2285.
https://doi.org/10.1182/blood-2005-06-2373

[14]   Kato, G.J., Martyr, S., Blackwelder, W.C., et al. (2005) Levels of Soluble Endothelium-Derived Adhesion Molecules in Patients with Sickle Cell Disease Are Associated with Pulmonary Hypertension, Organ Dysfunction, and Mortality. British Journal of Haematology, 130, 943-953.
https://doi.org/10.1111/j.1365-2141.2005.05701.x

[15]   Tabbara, I.A. (1992) Hemolytic Anemias. Diagnosis and Management. Medical Clinics of North America, 76, 649-668.
https://doi.org/10.1016/S0025-7125(16)30345-5

[16]   Lilford, R.J., Bentham, L., Girling, A., et al. (2013) Birmingham and Lambeth Liver Evaluation Testing Strategies (BALLETS): A Prospective Cohort Study. Health Technology Assessment, 17, 1-307.
https://doi.org/10.3310/hta17280

[17]   Ramachandran, R. and Kakar, S. (2009) Histological Patterns in Drug-Induced Liver Disease. Journal of Clinical Pathology, 62, 481-492.
https://doi.org/10.1136/jcp.2008.058248

[18]   Oyagbemi, A.A., Omobowale, T.O., Azeez, I.O., et al. (2013) Toxicological Evaluations of Methanolic Extract of M. oleifera Leaves in Liver and Kidney of Male Wistar Rats. Journal of Basic and Clinical Physiology and Pharmacology, 4, 307-312.
https://doi.org/10.1515/jbcpp-2012-0061

[19]   Bordon, Y. (2014) Microbiota: The Liver Debugs the System. Nature Reviews Immunology, 14, 430-431.
https://doi.org/10.1038/nri3704

[20]   Boulter, L., Lu, W.Y. and Forbes, S.J. (2013) Differentiation of Progenitors in the Liver: A Matter of Local Choice. The Journal of Clinical Investigation, 123, 1867-1873.
https://doi.org/10.1172/JCI66026

[21]   Abd-El Latif, A., El Bialy Bel, S., Mahboub, H.D., et al. (2014) M. oleifera Leaf Extract Ameliorates Alloxan-Induced Diabetes in Rats by Regeneration of β Cells and Reduction of Pyruvate Carboxylase Expression. Biochemistry and Cell Biology, 92, 413-419.
https://doi.org/10.1139/bcb-2014-0081

[22]   Sheikh, A., Yeasmin, F., Agarwal, S., et al. (2014) Protective Effects of M. oleifera Lam. Leaves against Arsenic-Induced Toxicity in Mice. Asian Pacific Journal of Tropical Biomedicine, 1, 353-358.
https://doi.org/10.12980/APJTB.4.201414B44

[23]   Pratt, D.S. and Kaplan, M.M. (2000) Evaluation of Abnormal Liver-Enzyme Results in Asymptomatic Patients. The New England Journal of Medicine, 342, 1266-1271.
https://doi.org/10.1056/NEJM200004273421707

[24]   Iwaisako, K., Jiang, C., Zhang, M., et al. (2014) Origin of Myofibroblasts in the Fibrotic Liver in Mice. Proceedings of the National Academy of Sciences, 111, 3297-3305.
https://doi.org/10.1073/pnas.1400062111

 
 
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