AJAC  Vol.2 No.7 , November 2011
A Method for the Measurement of Mercury in Human Whole Blood
Abstract: A method for measuring total mercury in human whole blood using Thermal Decomposi-tion-Amalgamation/ Atomic Absorption Spectrophotometry (TDA/AAS) was developed and applied to a study of women that were fish consumers. This method has a limit of detection of 0.33 μg/L. The blood mercury concentrations measured ranged from 0.74 μg/L to 14.80 μg/L, with a mean of 3.36 μg/L. Accuracy was within 15% of the expected value at the lower concentrations and within 10% at higher concentrations. Some 560 analysis were completed in about three weeks and the mean error in precision was 1.8% when measured in duplicate. It was concluded that this method is viable for use in clinical settings, with the benefit of small sample volumes and minimal sample preparation.
Cite this paper: nullA. Stube, H. Freiser and C. Santerre, "A Method for the Measurement of Mercury in Human Whole Blood," American Journal of Analytical Chemistry, Vol. 2 No. 7, 2011, pp. 752-756. doi: 10.4236/ajac.2011.27086.

[1]   T. W. Clarkson and L. Magos, “The Toxicology of Mer-cury and Its Chemical Compounds,” Critical Reviews in Toxicology, Vol. 36, No. 8, 2006, pp. 609-662. doi:10.1080/10408440600845619

[2]   E. Oken, J. S. Radesky, R. O. Wright, D. C. Bellinger, C. J. Amarasiriwardena, K. P. Kleinman, H. Hu and M. W. Gillman, “Maternal Fish Intake during Pregnancy, Blood Mercury Levels, and Child Cognition at Age 3 Years in a US Cohort,” American Journal of Epidemiology, Vol. 167, No. 10, 2008, pp. 1171-1191. doi:10.1093/aje/kwn034

[3]   L. W. Chang, “Toxicology of Metals,” Lewis Publishers, Boca Raton, 1996, p. 1052.

[4]   J. T. Ackerman, C. A. Eagles-Smith, J. Y. Takekawa, J. D. Bluso and T. L. Adelsbach,” Mercury Concentrations in Blood and Feathers of Prebreeding Foster’s Terns in Relation to Space Use of San Francisco Bay, California, Habitats,” Environmental Toxicology and Chemistry, Vol. 27, No. 4, 2008, pp. 897-908. doi:10.1897/07-230.1

[5]   APHA. 3112, “Metals by Cold-Vapor Atomic Absorption Spectrometry,” In: M. A. H. Franson, Ed., Standard Me-thods for the Examination of Water and Wastewater, Ame- rican Public Health Association, Washington D. C., Vol. A, 1998, pp. (3-22) - (3-24).

[6]   APHA. 3120, “Inductively Coupled Plasma (ICP) Me- thod,” In: M. A. H. Franson, Ed., American Public Health Association, Washington D.C., Vol. B, 1998, pp. (3-38) - (3-52).

[7]   D. D. Afonso, Z. Arsian and A. J. Bednar, “Assessment of Matrix-Dependent Analyte Stability and Volatility during Open-Vessel Sample Dissolution for Arsenic, Ca- dium, Mercury and Selenium,” Microchimica Acta, Vol. 167, No. 1-2, 2009, pp. 53-59. doi:10.1007/s00604-009-0218-3

[8]   EPA, “Method 7473: Mercury in Solids and Solutions by Thermal Decomposition, Amalgamation, and Atomic Adsorption Spectroscopy,” Agency UEP, 1998.

[9]   S. J. M. Butala, L. P. Scanlan and S. N. Chaudhuri, “A Detailed Study of Thermal Decomposition, Amalgama-tion/Atomic Absorption Spectrophotometry Methodology for the Quantitative Analysis of Mercury in Fish and Hair,” Journal of Food Protection, Vol. 69, 2006, pp. 2720-2728.

[10]   J. A. Lasrado, C. R. Santerre, S. M. Shim and J. R. Stahl, “Analysis of Mercury in Sportfish Tissue by Thermal Decomposition, Amalgamation/Atomic Absorption Spec-trophotometry,” Journal of Food Protection, Vol. 68, 20 05, pp. 879-881.

[11]   L. Trasande, P. J. Landrigan and C. Schechter, “Public Health and Economic Consequences of Methyl Mercury Toxicity to the developing Brain,” Environmental Health Perspectives, Vol. 113, 2005, pp. 590-596. doi:10.1289/ehp.7743