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 ABC  Vol.8 No.1 , February 2018
Distinct Gene Expression Profile Distinguishes Increased Metabolic Activity in Spontaneously Hyperactive Rats While Sedentary from That Induced by Exercise
Abstract: The Spontaneously-Running Tokushima Shikoku (SPORTS) strain is an original line derived from Wistar rats, which spontaneously runs >6 km/day on wheels, and has better glucose tolerance and less fat than Wistar rats. However, the molecular mechanism that contributes to the increased metabolic activity in SPORTS rats is unknown. The present study aimed to characterize the gene expression profiles of skeletal muscles in SPORTS rats housed under sedentary (SED) conditions. We found that the expression levels of genes encoding mitochondrial respiratory chain enzymes such as ATP synthase 6 (mt-Atp6) and cytochrome c oxidase subunit 6c (Cox6c), were higher in the soleus (SOL) muscles of SED SPORTS than in SED Wistar rats. The ratio of type IIa myofibers was higher and glucose tolerance was better in SED SPORTS than in Wistar rats that were sedentary and trained daily on treadmills, respectively. We then investigated candidate genes that might contribute to the better glucose tolerance of SED SPORTS rats using DNA microarray analysis. Among 116 upregulated genes in the SOL muscles of SED SPORTS rats, only 19 were also increased in trained Wistar rats. We focused on v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (Erbb3), which was associated with glucose transport in myocytes, and found higher expression levels in the SOL muscles of SED SPORTS than in SED Wistar rats. The SOL muscles of SED SPORTS rats also contained more activity of β-hydroxyacylCoA dehydrogenase, a key enzyme of β-oxidation, indicating enhanced lipid oxidation. These findings suggest that increased metabolic activity in skeletal muscle (especially the SOL muscle) of SPORTS rats is congenital and that gene expression profiles of SPORTS rats and Trained Wistar rats are different.
Cite this paper: Abe, M. , Matsuo, Y. , Harada-Sukeno, A. , Uchida, T. , Kitahata, K. , Tomida, C. , Hirasaka, K. , Teshima-Kondo, S. , Harada, N. , Nakaya, Y. , Sakaue, H. , Nakao, R. , Nikawa, T. (2018) Distinct Gene Expression Profile Distinguishes Increased Metabolic Activity in Spontaneously Hyperactive Rats While Sedentary from That Induced by Exercise. Advances in Biological Chemistry, 8, 1-14. doi: 10.4236/abc.2018.81001.
References

[1]   Morishima-Yamato, M., Hisaoka, F., Shinomiya, S., Harada, N., Matoba, H., Takahashi, A. and Nakaya, Y. (2005) Cloning and Establishment of a Line of Rats for High Levels of Voluntary Wheel Running. Life Sciences, 77, 551-561.
https://doi.org/10.1016/j.lfs.2004.10.074

[2]   Hattori, A., Mawatari, K., Tsuzuki, S., Yoshioka, E., Toda, S., Yoshida, M. and Nakaya, Y. (2010) β-Adrenergic-AMPK Pathway Phosphorylates Acetyl-CoA Carboxylase in a High-Epinephrine Rat Model, SPORTS. Obesity, 18, 48-54.
https://doi.org/10.1038/oby.2009.145

[3]   Pieter, L., Moreno, M., Silvestri, E., Lombardi, A., Goglia, F. and Lanni, A. (2007) Fuel Economy in Food-Deprived Skeletal Muscle: Signaling Pathways and Regulatory Mechanisms. The FASEB Journal, 21, 3431-3441.
https://doi.org/10.1096/fj.07-8527rev

[4]   Schiaffino, S. and Reggiani, C. (2011) Fiber Types in Mammalian Skeletal Muscles. Physiological Reviews, 91, 1447-1531.
https://doi.org/10.1152/physrev.00031.2010

[5]   Mizunoya, W., Wakamatsu, J.I., Tatsumi, R. and Ikeuchi, Y. (2008) Protocol for High-Resolution Separation of Rodent Myosin Heavy Chain Isoforms in a Mini-Gel Electrophoresis System. Analytical Biochemistry, 377, 111-113.
https://doi.org/10.1016/j.ab.2008.02.021

[6]   Nikawa, T., Ishidoh, K., Hirasaka, K., Ishihara, I., Ikemoto, M., Kano, M., Kominami, E., Nonaka, I., Ogawa, T., Adams, G.R., Baldwin, K.M., Yasui, N., Kishi, K. and Takeda, S. (2004) Skeletal Muscle Gene Expression in Space-Flown Rats. The FAS EB Journal, 18, 522-524.
https://doi.org/10.1096/fj.03-0419fje

[7]   http://www.subio.jp/

[8]   Gao, X., Zhao, X.L., Zhu, Y.H., Li, X.M., Xu, Q., Lin, H.D. and Wang, M.W. (2011) Tetramethylpyrazine Protects Palmitate-Induced Oxidative Damage and Mitochon- drial Dysfunction in C2C12 Myotubes. Life Sciences, 88, 803-809.
https://doi.org/10.1016/j.lfs.2011.02.025

[9]   Coderre, L., Vallega, G.A., Pilch, P.F. and Chipkin, S.R. (2007) Regulation of Glycogen Concentration and Glycogen Synthase Activity in Skeletal Muscle of Insulin- Resistant Rats. Archives of Biochemistry and Biophysics, 464, 144-150.
https://doi.org/10.1016/j.abb.2007.04.012

[10]   Holloway, G.P., Lally, J., Nickerson, J.G., Alkhateeb, H., Snook, L.A., Heigenhauser, G.J., Calles-Escandon, J., Glatz, J.F., Luiken, J.J., Spriet, L.L. and Bonen, A. (2007) Fatty Acid Binding Protein Facilitates Sarcolemmal Fatty Acid Transport But Not Mitochondrial Oxidation in Rat and Human Skeletal Muscle. Journal of Physiology, 582, 393-405.
https://doi.org/10.1113/jphysiol.2007.135301

[11]   Hood, D.A. (2009) Mechanisms of Exercise-Induced Mitochondrial Biogenesis in Skeletal Muscle. Applied Physiology, Nutrition, and Metabolism, 34, 465-472.
https://doi.org/10.1139/H09-045

[12]   Dimauro, S. and Schon, E.A. (2003) Mitochondrial Respiratory-Chain Diseases. New England Journal of Medicine, 348, 2656-2668.
https://doi.org/10.1056/NEJMra022567

[13]   Wu, Z., Puigserver, P., Andersson, U., Zhang, C., Adelmant, G., Mootha, V., Troy, A., Cinti, S., Lowell, B., Scarpulla, R.C. and Spiegelman, B.M. (1999) Mechanisms Controlling Mitochondrial Biogenesis and Respiration through the Thermogenic Coactivator PGC-1. Cell, 98, 115-124.

[14]   Cantó, C., Suárez, E., Lizcano, J.M., Grinó, E., Shepherd, P.R., Fryer, L.G., Carling, D., Bertran, J., Palacín, M., Zorzano, A. and Gumà, A. (2004) Neuregulin Signaling on Glucose Transport in Muscle Cells. Journal of Biological Chemistry, 279, 12260- 12268.
https://doi.org/10.1074/jbc.M308554200

[15]   Lin, J., Puigserver, P., Donovan, J., Tarr, P. and Spiegelman, B.M. (2002) Peroxisome Proliferator-Activated Receptor γ Coactivator 1β (PGC-1β), a Novel PGC-1- Related Transcription Coactivator Associated with Host Cell Factor. Journal of Biological Chemistry, 277, 1645-1648.
https://doi.org/10.1074/jbc.C100631200

[16]   Spangenburg, E.E. and Booth, F.W. (2003) Molecular Regulation of Individual Skeletal Muscle Fibre Types. Acta Physiologica Scandinavica, 178, 413-424.
https://doi.org/10.1046/j.1365-201X.2003.01158.x

[17]   Arany, Z., Lebrasseur, N., Morris, C., Smith, E., Yang, W., Ma, Y., Chin, S. and Spiegelman, B.M. (2007) The Transcriptional Coactivator PGC-1β Drives the Formation of Oxidative Type IIX Fibers in Skeletal Muscle. Cell Metabolism, 5, 35-46.
https://doi.org/10.1016/j.cmet.2006.12.003

[18]   Perseghin, G., Price, T.B., Petersen, K.F., Roden, M., Cline, G.W., Gerow, K., Rothman, D.L. and Shulman, G.I. (1996) Increased Glucose Transport-Phosphorylation and Muscle Glycogen Synthesis after Exercise Training in Insulin-Resistant Subjects. New England Journal of Medicine, 335, 1357-1362.
https://doi.org/10.1056/NEJM199610313351804

 
 
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