ACES  Vol.1 No.4 , October 2011
Determination of External Mass Transfer Model for Hydrolysis of Jatropha Oil Using Immobilized Lipase in Recirculated Packed-Bed Reactor
Abstract: In this study, a simple and effective technique for establishing an external mass transfer model in a recirculated packed-bed batch reactor (RPBBR) with an immobilized lipase enzyme and Jatropha oil system is presented. The external mass transfer effect can be represented with a model in the form of Colburn factor JD = K Re-(1–n). The value of K and n were derived from experimental data at different mass flow rates.The experiment shows an average increment of 1.51% FFA for calcium alginate and 1.62% FFA for carrageenan after the hydrolysis took place. Based on different biopolymer material used in immobilized beads, JD = 1.674 Re-0.4 for calcium alginate and JD = 1.881 Re-0.3 for k-carrageenan were found to be adequate to predict the experimental data for external mass transfer in the reactor in the Reynolds number range of 0.2 to 1.2. The purposed model can be used for the design of industrial bioreactor and scale up. Besides, the external mass transfer coefficients for the hydrolysis of Jatropha oil reaction and the entrapment efficiency for the two biopolymer materials used were also investigated.
Cite this paper: nullC. Cheng, R. Abdulla, R. Sridhar and P. Ravindra, "Determination of External Mass Transfer Model for Hydrolysis of Jatropha Oil Using Immobilized Lipase in Recirculated Packed-Bed Reactor," Advances in Chemical Engineering and Science, Vol. 1 No. 4, 2011, pp. 289-298. doi: 10.4236/aces.2011.14040.

[1]   American Oil Chemists’ Society, “AOCS Official Method Ca5a-40, Free Fatty Acid,” Champaign, lll, 1993.

[2]   Y. H. Chew, T. L. Chew, M. R. Sarmidi, R. A. Aziz and F. Razali, “External Mass Transfer Model for the Hydrolysis of Palm Olein Using Immobilized Lipase,” Food and Bioproducts Processing, Vol. 86, No. 4, 2008, pp. 276- 282. doi:10.1016/j.fbp.2008.02.001

[3]   P. D. Desai, A. M. Dave and S. Devi, “Entrapment of Lipase into K-Carrageenan Beads and Its Use in Hydrolysis of Olive Oil in Biphasic System,” Journal of Molecular Catalysis B: Enzymatic, Vol. 31, No. 4-6, 2004, pp. 143-150. doi:10.1016/j.molcatb.2004.08.004

[4]   N. Dizge and B. Tansel, “External Mass Transfer Analysis for Simultaneous Removal of Carbohydrate and Protein by Immobilized Activated Sludge Culture in a Packed Bed Batch Bioreactor,” Journal of Hazardous Materials, Vol. 184, No. 1-3, 2010, pp. 671-677. doi:/10.1016/j.jhazmat.2010.08.090

[5]   H. Hermansyah, A. Wijanarko, Dianursanti, M. Gozan, P. P. D. K. Wulan, R. Arbianti, R. W. Soemantojo, T. S. Utami, Yuliusman, M. Kubo, N. Shibasaki-Kitakawa and T. Yonemoto, “Kinetic Model for Triglyceride Hydrolysis Using Lipase: Review,” Makara, Seri Teknologi, Vol. 11, No. 1, 2007, pp. 30-35.

[6]   M. N. Kathiravan, R. K. Rani, R. Karthick and K. Muthukumar, “Mass Transfer Studies on the Reduction of Cr (VI) Using Calcium Alginate Immobilized Bacillus sp. In Packed Bed Reactor,” Bioresource Technology, Vol. 101, No. 3, 2010, pp. 853-858. doi:/10.1016/j.biortech.2009.08.088

[7]   M. Y. Liauw, F. A. Natan, P. Widiyanti, D. Ikasari, N. Indraswati and F. E. Soetaredjo, “Extraction of Neem oil (Azadirachta Indica A. Juss) Using N-Hexane and Ethanol: Studies of Oil Quality, Kinetic and Thermodynamic,” ARPN Journal of Engineering and Applied Sciences, Vol. 3, No. 3, 2008, pp. 49-54.

[8]   O. H. Lowry, N. J. Rosebrough, A. L. Farr and R. J. Randall, “Protein Measurement with the Folin-Phenol Reagents,” The Journal of Biological Chemistry, Vol. 193, No. 1, 1951, pp. 265-275.

[9]   T. Murugesan and R. Y. Sheeja, “A Correlation for the Mass Transfer Coef?cients during the Biodegradation of Phenolic Ef?uents in a Packed Bed Reactor,” Separation and Puri?cation Technology, Vol. 42, No. 2, 2005, pp. 103-110. doi:10.1016/j.seppur.2004.06.008

[10]   S. Shah, A. Sharma and M. N. Gupta, “Extraction of Oil from Jatropha Curcas L. Seed Kernels by Enzyme Assisted Three Phase Partitioning,” Industrial Crops and Products, Vol. 20, No. 3, 2004, pp. 275-279. doi:10.1016/j.indcrop.2003.10.010

[11]   J. C. Wu, V. Selvam, H. H. Teo, C. Yvonne, M. M. R. Talukder and W. J. Choim, “Immobilization of Candida rugosa Lipase by Cross-Linking with Glutaraldehyde Gollowed by Entrapment in Alginate Beads,” Biocatalysis and Biotransformation, Vol. 24, No. 5, 2004, pp. 352- 357. doi:10.1080/10242420600787326