AiM  Vol.7 No.12 , December 2017
Utilization of Ceramic Beads for Edible Mushrooms Cultivation
Abstract: This study examined ceramic beads as a reusable material for cultivation of edible mushrooms. There are 20 species of popular edible mushrooms in Japan all of which were tested. Within the cultivation vessels, 70% were ceramic beads (diameter 1 cm) and 30% of the nutrient solution. Moreover, the control groups used several types of sawdust, wheat bran, and rice bran with the ratio of 8:1:1 as the substrate. Two sets of substrates were evaluated with the fruit bodies yield. The result indicated that there were 11 species that responded well with the ceramic bead substrate when compared to the traditional sawdust substrate with Agrocybe cylindrica and Pleurotus ostreatus performed the best adding 70 g more of the fruit bodies. Conversely, nine species responded poorly with the ceramic beads substrate with Auricularia polytricha performed the worst losing 120 g. Ceramic beads as a reusable material for substrates not only provide a clean and controllable environment for mycelium to colonize but also deliver more aeration and water availability inside the cultivation vessels. The application of the ceramic bead cultivation can be viewed as an alternative solution for producing Ophiocordyceps sinensis on the industrial level.
Cite this paper: Huang, P. and Ohga, S. (2017) Utilization of Ceramic Beads for Edible Mushrooms Cultivation. Advances in Microbiology, 7, 853-862. doi: 10.4236/aim.2017.712065.

[1]   Kumar, K. (2015) Role of Edible Mushroom as Functional Foods—A Review. South Asian Journal of Food Technology and Environment, 1, 211-218.

[2]   Khatun, S., Islam, A., Cakilcioglu, U. and Chatterjee, N.C. (2012) Research on Mushroom as a Potential Source of Nutraceuticals: A Review on Indian Perspective. American Journal of Experimental Agriculture, 2, 47-73.

[3]   Zion Market Research (2017) Global Mushroom Market Is Expected to Reach above USD 59.48 Billion in 2021. Zion Market Research.

[4]   Zied, D.C. and Pardo-Giménez, A. (2017) Technology of Mushroom Cultivation. Edible and Medicinal Mushrooms: Technology and Applications. Wiley-Blackwell, New Jersey, 64-79.

[5]   Upadhyay, R.C. and Singh, M. (2011) Production of Edible Mushrooms. In: Hofrichter, M., Ed., Industrial Applications. The Mycota (A Comprehensive Treatise on Fungi as Experimental Systems for Basic and Applied Research), 10th Edition, Springer, Berlin, Heidelberg, 79-97.

[6]   de Carvelho, C.S.M., Sales-Campos, C. and de Andrade, M.C.N. (2010) Mushrooms of the Pleurotus Genus: A Review of Cultivation Techniques. Interciencia, 35, 177-182.

[7]   Vieira, F.R. and de Andrade, M.C.N. (2016) Optimization of Substrate Preparation for Oyster mushroom (Pleurotus ostreatus) Cultivation by Studying Different Raw Materials and Substrate Preparation Conditions (Composting: phases I and II). World Journal of Microbiology and Biotechnology, 32, 190.

[8]   Chang, S.T. and Miles, P.G. (2004) Mushrooms: Cultivation, Nutritional Value, Medicinal Effect, and Environmental Impact. CRC Press.

[9]   Hernández, D., Sánchez, J.E. and Yamasaki, K. (2003) A Simple Procedure for Preparing Substrate for Pleurotus ostreatus Cultivation. Bioresource Technology, 90, 145-150.

[10]   Philippoussis, A., Diamantopoulou, P., Papadopoulou, K., Lakhtar, H., Roussos, S., Parissopoulos, G. and Papanikolaou, S. (2010) Biomass, Laccase and Endoglucanase Production by Lentinula edodes during Solid State Fermentation of Reed Grass, Bean Stalks and Wheat Straw Residues. World Journal of Microbiology and Biotechnology, 27, 285-297.

[11]   Philippoussis, A., Diamantopoulou P. and Zervakis G. (2003) Correlation of the Properties of Several Lignocellulosic Substrates to the Crop Performance of the Shiitake Mushroom Lentinula edodes. World Journal of Microbiology and Biotechnology, 19, 551-557.

[12]   Chiu, S.W., Law, S.C., Ching, M.L., Cheung, K.W. and Chen, M.J. (2000) Themes for Mushroom Exploitation in the 21st Century: Sustainability, Waste Management, and Conservation. The Journal of General and Applied Microbiology, 46, 269-282.

[13]   Rizki, M. and Tamai, Y. (2011) Effects of Different Nitrogen Rich Substrates and Their Combination to the Yield Performance of Oyster Mushroom (Pleurotus ostreatus). World Journal of Microbiology and Biotechnology, 27, 1695-1702.

[14]   Brunnert, H. and Zadrazˇil, F. (1983) The Translocation of Mercury and Cadmium into the Fruiting Bodies of Six Higher Fungi: A Comparative Study on Species Specificity in Five Lignocellulolytic Fungi and the Cultivated Mushroom Agaricusbisporus. European Journal of Applied Microbiology and Biotechnology, 17, 358-364.

[15]   Fischer, R.G., Rapsomanikis, S., Andreae, M.O. and Baldi, F. (1995) Bioaccumulation of Methylmercury and Transformation of Inorganic Mercury by Macrofungi. Environment Science & Technology, 29, 993-999.

[16]   Droce, A., Sørensen, J.L., Giese, H. and Sondergaard, T.E. (2013) Glass Bead Cultivation of Fungi: Combining the Best of Liquid and Agar Media. Journal of Microbiological Methods, 94, 343-346.

[17]   Ohga, S. (1990) Growth Rate of Mycelium of Shiitake, Lentinusedodes, in Relation to Water Potential of Medium. Journal of the Faculty of Agriculture, Kyushu University, 34, 413-420.

[18]   Badham, E.R. (1989) Influence of Water Potential on Growth of Shiitake Mycelium. Mycologia, 81, 464-468.

[19]   Ohga, S. (1999) Effect of Water Potential on Fruit Body Formation of Lentinula edodes in Sawdust-Based Substrate. Journal of Wood Science, 45, 337-342.

[20]   Nguyen, L., Kalachova, L., Novotna, J., Holub, M., Kofronova, O., Benada, O., Thompson, C. and Weiser, J. (2005) Cultivation System using Glass Beads Immersed in Liquid Medium Facilitates Studies of Streptomyces Differentiation. Applied and Environmental Microbiology, 71, 2848-2852.

[21]   Redecker, D., Thierfelder, H. and Werner, D. (1995) A New Cultivation System for Arbuscular Mycorrhizal Fungi on Glass Beads. Angewandte Botanik, 69, 189-191.

[22]   Chen, B.D., Christie, P. and Li, X.L. (2001) A Modified Glass Bead Compartment Cultivation System for Studies on Nutrient and Trace Metal Uptake by Arbuscular Mycorrhiza. Chemosphere, 42, 185-192.

[23]   Dong, C.H. and Yao, Y.J. (2005) Nutritional Requirements of Mycelial Growth of Cordyceps sinensis in Submerged Culture. Journal of Applied Microbiology, 99, 483-492.

[24]   Zhou, X.W., Gong, Z.H., Su, Y., Lin, J. and Tang, K.X. (2009) Cordyceps Fungi: Natural Products, Pharmacological Functions and Developmental Products. Journal of Pharmacy and Pharmacology, 61, 279-291.

[25]   Zhou, X.W., Li, L.J. and Tian, E.W. (2014) Advances in Research of the Artificial Cultivation of Ophiocordyceps sinensis in China. Critical Reviews in Biotechnology, 34, 233-243.

[26]   Kuo, Y.C., Tsai, W.J., Shiao, M.S., Chen, C.F. and Lin, C.Y. (1996) Cordyceps sinensis as an Immunomodulatory Agent. The American Journal of Chinese Medicine, 24, 111-125.

[27]   Tuli, H.S., Sandhu, S.S. and Sharma, A.K. (2014) Pharmacological and Therapeutic Potential of Cordyceps with Special Reference to Cordycepin. 3 Biotech, 4, 1-12.

[28]   Zhong, S., Pan, H., Fan, L., Lv, G., Wu, Y., Parmeswaran, B., Pandey, A. and Soccol, C.R. (2009) Advances in Research of Polysaccharides in Cordyceps Species. Food Technology and Biotechnology, 47, 304-312.

[29]   Chioza, A. and Ohga, S. (2013) Mycelial Growth of Paecilomyces hepiali in Various Agar Media and Yield of Fruit Bodies in Rice Based Media. Advances in Microbiology, 3, 529-536.

[30]   Chioza, A. and Ohga, S. (2014) Effects of Hot-Water Extract of Paecilomyces hepiali on Hypertension Parameters in Spontaneously Hypertensive Rats. Advances in Microbiology, 4, 436-443.

[31]   Chioza, A. and Ohga, S. (2014) A Comparative Study on Chemical Composition and Pharmacological Effects of Paecilomyces hepiali and Wild Ophiocordyceps sinensis. Advances in Microbiology, 4, 839-848.