JACEN  Vol.6 No.4 , November 2017
Multi-Residue Analysis of Organophosphorus Pesticides in Vegetable Using GC-MS
Abstract: This study investigates the levels of pesticide residues in Cucumbers (Cucumis sativus), potatoes (Solanum tuberosum subsp. Tuberosum) and tomatoes (Lycopersicon esculentum). The samples were minced in a food processor initially subjected to extraction followed by cleanup using solid phase extraction (SPE) column. Separation was performed on a GC capillary column (Rtx-5 ms-30 m × 0.25 mm× 0.25 μm). The sample was injected by using splitless mode, helium as the carrier gas followed by quadrupole mass spectrometry detection. The mobile phase flow rates, column temperatures, and MS parameters were all optimized to reach high sensitivity and selectivity. Seven pesticides were detected in tomatoes sample, six in cucumber and four in potatoes samples. The findings indicate risks and concerns for public health.
Cite this paper: Elhag, D. , Abdalla, B. , Suliman, S. and Ali, I. (2017) Multi-Residue Analysis of Organophosphorus Pesticides in Vegetable Using GC-MS. Journal of Agricultural Chemistry and Environment, 6, 232-241. doi: 10.4236/jacen.2017.64016.

[1]   Kachuri, L., Harris, M.A., MacLeod, J.S., Tjepkema, M., Peters, P.A. and Demers, P.A. (2017) Cancer Risks in a Population-Based Study of 70,570 Agricultural Workers: Results from the Canadian Census Health and Environment Cohort (CanCHEC). BMC Cancer, 17, 343.

[2]   Prado, J.B., Mulay, P.R., Kasner, E.J., Bojes, H.K. and Calvert, G.M. (2017) Acute Pesticide-Related Illness among Farmworkers: Barriers to Reporting to Public Health Authorities. Journal of Agromedicine, 22, 395-405.

[3]   Ambrus, á. (2016) International Harmonization of Food Safety Assessment of Pesticide Residues. Journal of Agricultural and Food Chemistry, 64, 21-29.

[4]   Struciński, P., Góralczyk, K., Czaja, K., Hernik, A., Korcz, W. and Ludwicki, J.K. (2007) Consumer Risk Assessment in Case of Maximum Residue Levels (MRLs) Violations in Food. Roczniki Panstwowego Zakladu Higieny, 58, 377-388.

[5]   Gutowski, L., Baginska, E., Olsson, O., Leder, C. and Kümmerer, K. (2015)Assessing the Environmental fate of S-Metolachlor, Its Commercial Product Mercantor Gold? and Their Photoproducts Using a Water-Sediment Test and in Silico Methods. Chemosphere, 138, 847-855.

[6]   Salvia, M.-.V, Jrad, A.B., Raviglione, D., Zhou, Y. and Bertrand, C. (2017) Environmental Metabolic Footprinting (EMF) vs. Half-Life: A New and Integrative Proxy for the Discrimination between Control and Pesticides Exposed Sediments in Order to Further Characterise Pesticides’ Environmental Impact. Environmental Science and Pollution Research, 1-7.

[7]   World Health Organization (2009) The WHO Recommended Classification of Pesticides by Hazard and Guidelines to Classification 2009.

[8]   European Commission (2015) Guidelines on Active Substances and Plant Protection Products—Food Safety.

[9]   Department for Environment, Food & Rural Affairs and Health and Safety Executive (2016) Expert Committee Pesticide Residues in Food Annual Report

[10]   Lu, J.L., Cosca, K.Z. and Del Mundo, J. (2010) Trends of Pesticide Exposure and Related Cases in the Philippines. Journal of Rural Medicine, 5, 153-164.

[11]   Salvatore, A.L., Castorina, R., Camacho, J., Morga, N., López, J., Nishioka, M., et al. (2015) Home-Based Community Health Worker Intervention to Reduce Pesticide Exposures to Farmworkers’ Children: A Randomized-Controlled Trial. Journal of Exposure Science & Environmental Epidemiology, 25, 608-615.

[12]   Coskun, R., Gundogan, K., Sezgin, G.C., Topaloglu, U.S., Hebbar, G., Guven, M., et al. (2015) A Retrospective Review of Intensive Care Management of Organophosphate Insecticide Poisoning: Single Center Experience. Nigerian Journal of Clinical Practice, 18, 644-650.

[13]   Sungur, M. and Güven, M. (2001) Intensive Care Management of Organophosphate Insecticide Poisoning. Critical Care (London, England), 5, 211-215.

[14]   Qin, Y., Zhang, J., Li, Y., Wang, Q., Wu, Y., Xu, L., et al. (2017) Automated Multi-Filtration Cleanup with Nitrogen-Enriched Activated Carbon Material as Pesticide Multi-Residue Analysis Method in Representative Crop Commodities. Journal of Chromatography A, 1515, 62-68.

[15]   Pang, G.-F., Fan, C.-L., Liu, Y.-M., Cao, Y.-Z., Zhang, J.-J., Li, X.-M., et al. (2006) Determination of Residues of 446 Pesticides in Fruits and Vegetables by Three-Cartridge Solid-Phase Extraction-Gas Chromatography-Mass Spectrometry and Liquid Chromatography-Tandem Mass Spectrometry. Journal of AOAC International, 89, 740-771.

[16]   Rezk, M.R., Abd El-Aleem, A.E.-A.B., Khalile, S.M. and El-Naggar, O.K. (2017) Determination of Residues of Diazinon and Chlorpyrifos in Lavender and Rosemary Leaves by Gas Chromatography. Journal of AOAC International.

[17]   Pérez-Fernández, V., Mainero Rocca, L., Tomai, P., Fanali, S. and Gentili, A. (2017) Recent Advancements and Future Trends in Environmental Analysis: Sample Preparation, Liquid Chromatography and Mass Spectrometry. Analytica Chimica Acta, 983, 9-41.

[18]   Grimalt, S. and Dehouck, P. (2016) Review of Analytical Methods for the Determination of Pesticide Residues in Grapes. Journal of Chromatography A, 1433, 1-23.

[19]   Danezis, G.P., Anagnostopoulos, C.J., Liapis, K. and Koupparis, M.A. (2016) Multi-Residue Analysis of Pesticides, Plant Hormones, Veterinary Drugs and Mycotoxins using HILIC Chromatography—MS/MS in Various Food Matrices. Analytica Chimica Acta, 942, 121-138.

[20]   Chen, X., Bian, Z., Hou, H., Yang, F., Liu, S., Tang, G., et al. (2013) Development and Validation of a Method for the Determination of 159 Pesticide Residues in Tobacco by Gas Chromatography-Tandem Mass Spectrometry. Journal of Agricultural and Food Chemistry, 61, 5746-5757.

[21]   Campos-Manas, M.C., Plaza-Bolanos, P., Sánchez-Pérez, J.A., Malato, S. and Agüera, A. (2017) Fast Determination of Pesticides and Other Contaminants of Emerging Concern in Treated Wastewater using Direct Injection Coupled to Highly Sensitive Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry. Journal of Chromatography A, 1507, 84-94.

[22]   EU Pesticides Database European Commission [Internet].

[23]   Chlornitrofen Toxicity, Ecological Toxicity and Regulatory Information [Internet].

[24]   Kitamori, S. (1996) [Biodegradation of Herbicide Chlornitrofen (CNP) and Mutagenicity of Its Degradation Products.] Fukuoka Igaku Zasshi Hukuoka Acta Medica, 87, 142-150.

[25]   EPN Chemical Profile 1/88 [Internet].

[26]   Matsushita, T., Matsui, Y., Matsui, Y. and Inoue, T. (2005) Fate of Mutagenicity Produced during Anaerobic Biodegradation of the Herbicide Chlornitrofen (CNP). Journal of Environmental Science and Health, Part B, 40, 851-861.

[27]   EPN Toxicity, Ecological Toxicity and Regulatory Information [Internet].

[28]   Ribeiro Begnini Konatu, F., Breitkreitz, M.C. and Sales Fontes Jardim, I.C. (2017) Revisiting Quick, Easy, Cheap, Effective, Rugged, and Safe Parameters for Sample Preparation in Pesticide Residue Analysis of Lettuce by Liquid Chromatography-Tandem Mass Spectrometry. Journal of Chromatography A, 1482, 11-22.

[29]   Trevisan, M.T.S., Owen, R.W., Calatayud-Vernich, P., Breuer, A. and Picó, Y. (2017) Pesticide Analysis in Coffee Leaves using a Quick, Easy, Cheap, Effective, Rugged and Safe Approach and Liquid Chromatography Tandem Mass Spectrometry: Optimization of the Clean-Up Step. Journal of Chromatography A, 1512, 98-106.

[30]   Martins, M.L., Kemmerich, M., Prestes, O.D., Maldaner, L., Jardim, I.C.S.F. and Zanella, R. (2017) Evaluation of an Alternative Fluorinated Sorbent for Dispersive Solid-Phase Extraction Clean-Up of the Quick, Easy, Cheap, Effective, Rugged, and Safe Method for Pesticide Residues Analysis. Journal of Chromatography A, 1514, 36-43.