Urbanization and increasing industrialization of cities result in an increase in the load of wastewater that is enriched in organic matter and dissolved substances. The outcome is a deterioration of water quality, reduction of diversity, and on a long-term, the disappearance of water bodies  . Artificial lakes in Cameroon are increasingly faced with sedimentation problems. While many studies have been carried out on the filling of lakes in Europe and America in general, little is known in Africa  . In Cameroon, some works done by Zébazé Togouet et al.  , Foto Menbohan et Njine  show respectively that, the Yaoundé municipal and the Effoulan lakes represent major assets to support the country’s development but they are threatened by hydrological changes, pollution and siltation problems.
Siltation and eutrophication of lakes cause flooding, significant changes in the physicochemical and biological parameters and consequently rapid growth of aquatic plants  . The Nkolbisson artificial lake was created in the 1970s by the Higher National School of Agriculture in order to facilitate the training of engineering students in various disciplines (viticulture, irrigation experimental fields, fishing competitions and water skiing). After the closure of the school, all activities were definitely stopped in 1980. The continuous drainage into the lake of untreated wastewater from the Abiergue and Mintotomo streams of the Mefou watershed flowing across Cité Verte from the rivers of the Mefou watershed and the Abiergue, Mintotomo, Cité verte located upstream, as well as the wastewater treatment plant of Camps Sic Cité verte, has gradually degraded its quality and environment  . Despite the dangers inherent in the filling of lakes over time, no study has yet been undertaken on the Nkolbisson artificial lake; one of the oldest artificial lakes in Yaoundé Bessenassé  . Okasha et al.  indicate that siltation of lakes results in the reduction of their lifespan, one of the most harmful effects of water erosion. The management of lake ecosystems, therefore, requires a thorough knowledge of the hydrodynamical and sedimentary operation to propose scenarios of changes that are adapted to different environmental constraints. These include maintenance of water quality, improvement of fish habitat and aquatic organisms habitat, protection of structures, development of recreational and ecotourism activities   . This study is one of the first studies that focus on the filling of the Nkolbisson artificial lake. This work aims at diagnosing the parameters that contribute to the degradation and filling rate of the Nkolbisson artificial lake and to propose approaches to ensure its restoration and sustainable management. Therefore, it is necessary to avoid refilling the lake that disequilibrates the Nkolbisson artificial lake which represents a touristic site to the urban community.
2. Geological Setting
The studied lake (3˚87'N and 11˚52'E), is located at Nkolbisson within the Yaoundé VII Sub-Division (Figure 1). The vegetation is made up of forest, Sterculiaceae and Ulmaceae which is highly degraded by urbanization  . The
Figure 1. Map of the Nkolbisson artificial lake indicating the different tributaries.
area is dominated by lateritic soils with minor hydromorphic subsoils  . The hydrographic network is dense, made up of many low and marshy funds including the Mefou River which is flowing through the southern part of the Nkolbisson neighborhood. The climate is characterized by two rainy seasons (September to mid-November and mid-March to June) with an annual average temperature of 23.5˚C.
3. Analytical Methods
The choice of the sampling points was not easy due to many factors including the rough morphology of the studied area, the multiplicity and the diversity of wastes. However, basing on the source of materials flowing into the lake, three water sampling points representing the streams feeding the studied lake were chosen: 1/Abiergue which is, situated upstream of the Lake, 2/Mintotomo, main tributary of Abiergue which crosses the undeveloped rural area and 3/the outlet of the studied lake (Figure 1). Samples were collected both during raining season (two sets of sampling) dry season (two sets) within the period 2013 to 2014. Given that the biodegradation of suspended matters in waste water takes place at a very fast rate, physicochemical parameters including temperature settings (T), pH, dissolved oxygen (DO) and electrical conductivity (EC) were measured using a mercury thermometer, pH meter (Schott CG 818 Geräte GmbH), an Oximeter (OXI model 315i WTW) and conductivity meter mark HACH, respectively. Turbidity was measured using turbidity meter (HANNA instrument model LP 2000) at LAGE Laboratory, Yaoundé. Suspended solids (SS) were measured using a brand of Millipore filtration ramp WHATMAN GF/F 47 mm circular diameter with porosity 0.45 µm. The liquid debit was estimated by the volumetric method and the solid debit was calculated from the average concentration of suspended solid (SS) using the equation.
where Qss, represents the debit of suspended solid (kg・s−1); QL, the debit of liquid (m3・s−1) and average of concentration of suspended solids (g・L−1).
The annual flux of suspended solids transported by the Abiergue and Mintotomo was finally obtained by summing up the solid input of the stations which supply the lake.
The liquid flow rate was also estimated by the volumetric method. The Total Suspended Solids was calculated from the weighted average concentrations of Suspended matters by liquid flow rates  the equation.
QSS: representing the suspended solids discharge (kg・s−1);
QL: the liquid flow (m3・s−1)
C: the average concentration of suspended solids (g・L−1).
The annual flux of suspended solids transported by the Abiergue and Mintotomo Rivers was finally obtained by adding the solid contributions of the two stations that feed the lake. BOD was determined by the breathing method using a brand-meter (WTW BOD Model 1020T) using the technique described by Din  . COD was determined by oxidation in a potassium medium at a temperature of 148˚C, the oxidizable material under the conditions of the test in the presence of silver sulphate as catalyst and mercury sulfate. For bacteriological analysis, water samples were collected in sterile 0.5 l container and the enumeration of bacteria indicative of faecal contamination: total coliforms (TC), faecal coliforms (FC) and faecal streptococci (FS), were performed by filtration through a membrane filter of porosity 0.45 µm. For much polluted water, a series of dilutions (10−4 to 10−9) were conducted. The culture of (TC) was carried out on middle Tergitol 7 agar TTC and incubation at 37˚C during 24 hours. The culture of (FC) was also performed on middle Tergitol 7 agar VAT, with an incubation at 44˚C for 24 hours. The enumeration of FS was performed on Slanetz and Bartley after incubation at 37˚C for 24 hours.
4. Results and Discussion
4.1. Physicochemical Analysis
Temperatures (T˚C) did not vary a lot with the seasons, but was generally higher in the dry season, with values ranging between 23.2˚C and 23.9˚C compared to the rainy season (23.1˚C - 23.3˚C) (Figure 2). According to Moumouni Djermakoye  , high temperatures in water promote the development of microor-
Figure 2. Seasonal variation in temperature.
ganisms, induces unpleasant odour and increases the sedimentation rate of suspended matter. The results of this study are similar to those of Akono Ntonga et al.  . Water temperature plays an important role in the solubility of gases, the separation parting of dissolved salts and pH determination. Water temperature is also important to know the origin of the water and to identify the mixing of different water types is influenced by the source from where they originate  .
Based on the pH of the lake water that ranged from 6.90 to 7.20 (Figure 3), the lake is alkaline in nature. The highest pH values were obtained in the dry season. These results are similar with those obtained in other lakes such as the Yaoundé municipal lake  , the Ebolowa municipal lake  and the Mefou lake  . These values could be due to the degradation of organic matter (plants, animals, faeces and domestic waste water).
4.1.2. Dissolved Oxygen
Dissolved oxygen (DO) values showed no significant seasonal variation and were generally low (<6 mg/l). They were higher (4 to 5.2 mg/l) during the rainy season with the highest value recorded at the lake’s outlet while the lowest (3.8 mg/l) was along Abiergue River during the dry season (Figure 4). These results show a strong deficit in DO, with no significant seasonal variation, although we noticed a slight increase at the flood season outfall. Oxygen is known as a good indicator of pollution of waterways and the monitoring of its self-purification. At the studied sites, the DO was low, showing a low photosynthetic activity. This might be due to the extent of pollution of the lake. The average DO value obtained (<5.2 mg/l) was below the minimum (>6 mg/l) of DO characteristic of normal biological conditions  . These low DO values could be due to the influx of waste water enriched in organic matter and inorganic substances. The values further decreased in the lake due to the degradation of organic matter triggering the multiplication of aerobic microorganisms. If oxygen levels maintain at 3 mg/l, it could lead to the dead of fish  . Conversely, opposite results were observed in the Yaoundé municipal lake, (93.3 to 127.6 mg/l)  .
4.1.3. Electrical Conductivity
The measure of Electrical conductivity (EC) indicates the degree of water and
Figure 3. Seasonal variation of pH.
Figure 4. Seasonal variation of dissolved oxygen.
therefore the amount of ionized dissolved substances. Electrical conductivity varied between 90 and 260 µS/cm without a significant seasonal variation. The EC value in the lake was high during the dry season (279 μS/cm), moderate in Abiergue station (266.1 µS/cm during dry season to 260 μS/cm during rainy season) and low in Mintotomo (92.1 - 87.3 μS/cm) (Figure 5). Low concentrations observed at Mintotomo have been described in the Yaoundé  and Ebolowa  municipal lakes as indicator of an insufficient mineralization  and maybe due both to the poor silica-nature of the basement and the load of wastewater in urban areas  .
Turbidity (Turb.) values ranged between 10.2 and 22.4 NTU. The range of values was linked to season. The highest values (10.2 - 22.4 NTU) were observed during the dry season while lowest values (10 - 19 NTU) correspond to rainy season (Figure 6). There was no significant seasonal variation observed at the Mintotomo station. The high turbidity values (22.4 NTU) observed at Lake outlet as described in Taabo Lake in Ivory Coast  , may be linked both to highly charge water intakes from Abiergue and Mitotomo and also to particles transported during rainfall.
4.2. Bacteriological Analysis
4.2.1. Total Coliform
The temporal evolution of load in total coliforms (TC) in the three sampling sites showed significant fluctuation between seasons (Figure 7). The time course of faecal coliform concentrations showed that highest values (44 × 105 - 17 × 105 FCU/100 ml) were recorded during floods while during dry season, the levels were relatively low (15 × 104 - 4 × 105 FCU/100 ml). The highest concentration
Figure 5. Seasonal variation of electrical conductivity.
Figure 6. Seasonal variation of turbidity.
Figure 7. Seasonal variation of total coliforms.
(17 × 105 FCU/100 ml) was recorded in the rainy season at the lake outlet while the lowest (15 × 104 FCU/100 ml) was recorded at Mintotomo. This massive presence of total coliforms in water is due to the influx of domestic and industrial wastewater into rivers. The TC is known as an indicator of microbial quality of water because it is associated with faecal pollution  .
4.2.2. Faecal Coliform (FC)
Faecal coliform (FC) concentration varied from 10 × 102 to 425 × 106 FCU/ml. Its spatial variation is closely related to that of total coliform, confirming the water contamination in the studied site by faecal matter. The seasonal variation showed that concentrations varied between 10 × 102 and 425 × 106 FCU/100 ml during the dry season, with the highest and lowest contents respectively recorded at Abiergue and Mintotomo (Figure 8). Whereas during the rainy season, concentrations ranged from 420 to 32 × 106 FCU/100 ml, with the highest value
Figure 8. Seasonal variation of faecal coliforms.
found at Abiergue and the lowest is noted at the Mintotomo. These high concentrations might be due to discharges of either urban and domestic wastewater or faecal matter from shallow pit toilets directly into the runway as done by some of the surrounding population  .
4.2.3. Faecal Streptococci (FS)
The FS are associated with faecal coliform, and are considered as good indicators of pollution, and also of treatment because they are significantly more resistant than coliforms and other pathogenic enterobacteria  . The amount of FS was generally higher than that of FC in the studied area (Figure 9). At Mintotomo, values of FS were higher during the dry season (158 × 103 FCU/100 ml) compared to the rainy season (12 × 103 FCU/100 ml). At the Abiergue station, FS varied from 33 × 103 FCU/100 ml during the dry season to 19 × 103 FCU/100 ml during the rainy season. The same trend was observed at the lake with 8 × 103 FCU/100 ml during the dry season and 3 × 103 FCU/100 ml during the rainy season. The highest concentrations were found at the Abiergue and at the lake outlet respectively during the dry and the rainy seasons. This is explained by the accumulation of organic load during the dry season followed by their transport and storage within the lake during the rainy season. During the study period, FC concentration was lower than that of FS and TC compared to faecal coliforms; faecal streptococci are reputed to be resistant to toxic conditions. It has been presented that prolonged exposure to toxic elements and to critical conditions of the medium is more letal for faecal coliforms than faecal streptococci. Ajibade et al.  found opposite results in the National Park lake in Nigeria, may be due lack of a constant treatment of the lake water. In general, the results of the bacterial counts indicate a very poor microbiological water quality.
4.2.4. Determining the Origin of Faecal Contamination (R = FC/FS)
The quantification of faecal flora contamination (Table 1) allowed us to follow the evolution of the FC/FS ratio in the three studied sites. In general, this ratio varies from (0.23 to 3.97) in the samples assessed. The quantification of the contaminated faecal flora (Table 1) enabled us to follow the evolution of the ratio of faecal coliforms/faecal streptococci at the level of three sites studied. During the two seasons (dry and rainy), the ration CF/SF was between 2.2 and 3.83, and 3.81 and 3.97), respectively at the level of the lake outlet and of Abiergue. The
Figure 9. Seasonal variations of faecal streptococci.
Table 1. Origin of pollution according to FC/FS ratio.
values of these ratio characterise an origin of mix faecal contamination to human predominance since R falls between 2 < R < 4. This origin is linked to direct discharge of human excreta into streams by the population living along streams using them as privileged places for defecation  . The Mintotomo station either during low water or flood season was marked by contamination of animal origin (R < 0.7) linked to the presence of livestock and leaching of farmlands laden with manure. The discharge of untreated waste water and solid waste into rivers known as a common phenomenon might also be one of the most important sources of microbial pollution in the area. Because of the expansion of cities, hydrosystems currently become the main enabling environment of waterborne diseases  .
4.2.5. Biochemical Oxygen Demand (BOD5)
Biochemical oxygen demand (BOD) is the amount of dissolved oxygen consumed by microorganisms in the dark at 20˚C for 5 days. It enables the evaluation of biodegradable organic matter present in water; Figure 10 shows an increase of BOD in waters of the study area especially during dry periods. The average values of BOD varied from 8 mg/l (Mintotomo) and 30 mg/l (Abiergue) in times of floods and between 9 mg/l (Mintotomo) and 55 mg/l (Abiergue) during the dry season. The upstream part of the lake receives raw sewage enriched in organic matter and nutrients from urban centers including Cité verte, Mokolo, Nkomkana and Oyomabang. This causes a significant increase in the organic load of surface waters. The results obtained in this study indicate that the BOD values decrease from Abiergue to Mintotomo. The BOD contents are higher in the dry season compared to the rainy season. This may be due to the fact that during the dry season the flow velocity decreases and waters become warm,
Figure 10. Seasonal variation of biochemical oxygen demand.
providing suitable conditions for the degradation of organic matter by microorganisms. This activity consumes oxygen and results to the self-purification of water  . Though, during the rainy season, the rain water significantly contributes to the dilution of organic load from waste water. The Mintotomo station samples are weakly loaded because of its location far from the highly populated neighbourhoods. According to Emere et al.  , the waters of Abiergue and at the lake outlet are heavily polluted (BOD > 10 mg/l) while those of Mintotomo are moderately polluted (2 ≤ DBO5 ≤ 2.9 mg/l). The same results were found in the water of the Chikila Lake in Indian  .
4.2.6. Chemical Oxygen Demand (COD5)
The measurement of Chemical oxygen demand (COD) is suitable to monitor municipal and industrial wastewater. It is representative both of the bulk organic compounds and of oxidizable mineral salts (sulphides, and chlorides. The COD contents recorded for the studied waters ranged from 16.5 mg/l (Mintotomo) to 68.8 mg/l (Abiergue) and between 19.5 mg/l (Mintotomo) and 76 mg/l (Abiergue), respectively during rainy and dry seasons. No significant variation between seasons was observed at the lake outlet, (45 - 40 mg/l) (Figure 11). High COD values are related to the quality of wastewater and urban domestic water discharged (used as discharge outlet of different waste). The level of COD recorded throughout the study area was generally lower during the rainy season compared to the dry season. Furthermore, during the dry season the contents recorded in all stations are far beyond the WHO and UNICEF standards  of 30 mg/l. The increase of COD in the Abiergue station can be explained by the leaching of soils containing inorganic elements. It was noticed that during both the rainy and dry seasons the COD contents of Mintotomo waters remained low. According to Igbinosa and Okoh  , lower concentrations of COD during the dry season could be attributed to a reduction in organic and inorganic substances in the receiving environment.
5. Evaluation of Organic pollution
The ratios of COD/BOD, BOD/COD (Table 2) provide a very good means to evaluate the level of pollution of waste water and to optimize the physicochemical parameters of waste water which can be useful when proposing suitable treatment methods.
Figure 11. Seasonal variation of the chemical oxygen demand.
Table 2. Expression of the organic load.
5.1. Relationship COD/BOD
The ratio COD/BOD helps to determine the nature of the pollution of the receiving environment. According to Singh et al.  COD/BOD > 2 means that there is chemical pollution and requires biological treatment. In the present study, the COD/BOD of wastewater of the three studied sites (Abiergue, Mintotomo and Lake Outlet) indicates two categories of outcomes depending on the season. During the dry season, the COD/BOD ratio varied from 1.38 to 3.0 mg/l and during the rainy season it ranged from 2.06 to 3.08 mg/l (Table 2). These results characterize a chemical pollution which requires biological treatment. The values obtained (≤3) indicate chemical pollution, except Abiergue during the dry season. In fact, low values of the ratio COD/BOD involving the presence of a large proportion of biodegradable material and allow considering a biological treatment. Conversely, a large value of this ratio shows that much of the organic material is not biodegradable. Then, in this case, it is preferable to consider a physicochemical treatment. Similar values were observed in wastewater from the city of Oujda in Morocco  .
5.2. Relationship BOD/COD
When characterizing industrial pollution, the ratio BOD/COD is often used to provide insights on the origin of sewage pollution and its treatment options. In the present study, the ratio varies from 0.34 to 0.72 during dry season and from 0.35 to 0.48 in rainy season period (Table 2). The values greater than 0.3 during dry season are consistent with those obtained by Nayak et al.  , and are indicative of a strong inorganic pollution.
5.3. Solid Load
The liquid flow at the entrance of the artificial lake is 560 l/s) during the rainy season and 180 l/s during the dry season. These results are high compared to those observed in most lakes located near large municipalities. The suspended solids (SS) loads carried by the waters of the three sites experience significant seasonal fluctuations (Table 3). The solid load entering the lake varies from 128.4 g/l during the rainy season to 13.6 g/l during the dry season. While at the lake outlet, the solid load leaving the lake increases to 48 g/l during the rainy season and to 2.4 g/l during the dry season. The particulate loads are higher during the rainy season when the river flows and suspended matter experience a significant increase. During the course of the hydrological cycle, the particulate matter entering the lake (input) in the rainy season is approximately 4049.22 tons of suspended solids from the Abiergue and Mintotomo against 1513.73 tons of suspended matter at the outlet (output). While during the dry season, the suspended matters drops to 428.89 tons at the entrance of the lake (input) against 75.68 tons at the outlet (output). The increase in suspended solid load during the rainy season could be linked to the contributions from sediments loaded runoff water entering the lake. Bouanani  reports that the importance and nature of the suspended solid load are related either to the intensity of erosion by runoff water; thus, the amount of rainfall or to the ability of the flow conveying solids.
The eutrophication of the water body is characterized by the presence of algae and the development of several macrophytes such as Nymphea lotus, Paspalum vaginatum, Ceratophyllum demerson that occur during floods. During low flow periods, these macrophytes disappear under the effect of heat. Indeed, the growth of aquatic plants is causing the formation of a screen to the water surface which prevents the oxygenation in the middle of the lake during flood periods (Figure 12). Furthermore, the decomposition of macrophytes induces an increase in the amount of organic matter in the environment which the degradation therefore favors suffocation of fish species  . This may cause an imbalance in the trophic chain (production/consumption) of a body of water and lead to significant ecological consequences  .
Table 3. Seasonal variations in sediment loads suspended in the Nkolbisson artificial lake.
Figure 12. Seasonal variation of the suspended solids.
The discharge of domestic waste contributes strongly to organic enrichment of the Nkolbisson artificial lake because the particulate contributions of output are lower than those of input throughout the study (Table 3).
5.4. Correlation between Physicochemical Parameters
The correlation matrix (Table 4) shows that during low flow periods, there is a strong positive correlation both between temperature and fecal coliform (r = 0.90) and between total coliform (r = 0.90). These strong correlations are due to the fact that aboriginal bacteria are the dominant components of the total population in the polluted river waters. The electrical conductivity is strongly correlated with pH (r = 0.79) and suspended matters, due to soil runoff crossed with a strong presence of ions that induce a pH thus favoring the presence of suspended solids in the water.
There is also a strong correlation between the dissolved oxygen and fecal streptococci (r = 0.78), pH and turbidity (r = 0.87) and a very strong correlation between faecal coliform and total coliform (r = 0.99). Strong negative correlations are observed (between −0.60. −0.99) between temperature and pH (r = −0.6), temperature and turbidity (r = −0.91); conductivity and DO (−0.83), conductivity and FC (−0.99); pH and FS (−0.85); Strong negative correlations were also observed between DO and SS (r = −0.87), FC (r = −0.87) and FS (r = −0.87); and between SS and FS (−0.99). Negative correlations are observed between turbidity and FC (r = −0.65) and TC (r = −0.65).
During flood periods, the existing bond between all the variables in pairs and correlation coefficients between these variables were given by the correlation matrix. Table 5 shows that the temperature is strongly (T ˚C) correlated with the following: EC (0.99), pH (0.72), SS (0.94) and (TC) (0.95). The conductivity is strongly correlated with pH (0.82), SS (0.88), TC (0.89). The DO is strongly correlated with turbidity (0.91); TSS highly correlated with TC (0.99) and SF with TC (0.69). The strong significant relationship between temperature and suspended solids is probably related to the fact that the temperature observed during the flood period is favorable to the development of certain bacterial species. Strong negative correlations are also observed between pH and FC (−0.99), DO and FS (−0.99) and turbidity and FS (−0.94). The correlations between different variables highlight the similarity of the phenomena behind the release of these
Table 4. Pearson’s correlation coefficient between different parameters during the dry season.
Table 5. Pearson’s correlation coefficient between the different parameters during the rainy season.
elements in water.
An investigation into the chemical and biological characteristics of the Nkolbisson artificial lake has been carried out. The study reveals that, the water of the lake Abiergue station is turbid; with conductivity values are higher than 100 µS/cm. The results of COD reflect the importance of the content of organic matter at the lake which ranges from 76 mg/l during the dry season to 68 mg/l during the rainy season. The low values of BOD indicate the poor content in microorganisms of the waters. The analysis of total coliforms (TC), faecal coliforms (FC), faecal streptococci (FS) indicate pollution from urban districts upstream such as Cité verte Mokolo and Madagascar. The physicochemical and microbiological waters qualities of the study area are far from the WHO guidelines values, and are qualified to be very low. The filling rate of the lake by particulate matter is 2888.7 tons/year representing 64.5% of the total matter at the inlet. The lake is in an accelerated eutrophication process that led to the current stage of hypereutrophy. The imbalance of the ecosystem comes from its continuous supply of organic matters. This study is a scientific showcase and, therefore, a tool to the decision of the authorities of the Yaoundé VII District, from which strong decisions must be made to facilitate the restoration of this urban river system which is rich in aquatic biodiversity.
The present study has been funded by the Research Institute for Development “IRD” and the Norwegian Agency for Development (START/NORAD program). We thank Dr. Joseph Hell (Director of Institute of Geological and Mining Research―Cameroon) and Dr. Jean Claude Ntonga (Cameroon Head of Hydrological Research Centre) for providing logistics.
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