IJCM  Vol.8 No.11 , November 2017
Comparison of Pregnancy Outcomes of High-Quality D5- and D6-Blastocyst Transfer in Hormone-Replacement Frozen-Thawed Cycles
Abstract: This study aimed to assess pregnancy outcomes after high-quality D5- and D6-blastocyst transfer in frozen cycles of in vitro fertilization and embryo transfer and to further evaluate whether there was a difference in blastocyst development potentials with different developmental speeds and in pregnancy outcomes. A retrospective analysis was conducted to analyze 247 frozen cycles in our center from September 2015 to July 2017, which were divided into two groups: a D5-FET group with 193 cycles of D5-blastocyst transfer, and a D6-FET group with 54 cycles of D6-blastocyst transfer. Hormone replacement method was utilized to prepare frozen-cycle endometria. Pregnancy outcomes were analyzed and compared between these two groups. The mean ages of the two groups were 31.45 ± 4.43 years and 31.98 ± 4.84 years, respectively, with no statistically significant differences (P > 0.05). The difference in the endometrial thickness during transfer was also not statistically significant. The implantation rate in the D5-FET group was 60.13%, significantly higher than that in the D6-FET group (31.58%, P < 0.05). The clinical pregnancy rate in the D5-FET group was 68.91%, significantly higher than that in the D6-FET group (40.74%, P < 0.05). No statistically significant differences were found in the abortion rate and ectopic pregnancy rate between the two groups. The implantation, biochemical pregnancy, and clinical pregnancy rates of the blastocyst D5 were all superior to those of the blastocyst D6. In clinics, therefore, D5-blastocyst transfer could be prioritized for embryo transfer.
Cite this paper: Xing, W. , Cai, L. , Sun, L. and Ou, J. (2017) Comparison of Pregnancy Outcomes of High-Quality D5- and D6-Blastocyst Transfer in Hormone-Replacement Frozen-Thawed Cycles. International Journal of Clinical Medicine, 8, 565-571. doi: 10.4236/ijcm.2017.811053.

[1]   Maheshwari, A., Griffiths, S. and Bhattacharya, S. (2011) Global Variations in the Uptake of Single Embryo Transfer. Human Re-production Update, 17, 107-120.

[2]   El-Toukhy, T., Wharf, E., Walavalkar, R., Singh, A., Bolton, V., Khalaf, Y. and Braude, P. (2011) Delayed Blastocyst Development Does Not Influence the Outcome of Frozen-Thawed Transfer Cycles. International Journal of Obstetrics & Gynaecology, 118, 1551-1556.

[3]   Sunkara, S.K., Siozos, A., Bolton, V.N., Khalaf, Y., Braude, P.R. and El-Toukhy, T. (2010) The Influence of Delayed Blastocyst Formation on the Outcome of Frozen-Thawed Blastocyst Transfer: A Systematic Review and Meta-Analysis. Human Reproduction, 25, 1906-1915.

[4]   Hiraoka, K., Hiraoka, K., Miyazaki, M., Fukunaga, E., Horiuchi, T., Kusuda, T., Okano, S., Kinutani, M. and Kinutani, K. (2009) Perinatal Outcomes Following Transfer of Human Blastocysts Vitrified at Day 5, 6 and 7. Journal of Experimental & Clinical Assisted Reproduction, 6, 4.

[5]   Mesut, N., Ciray, H.N., Mesut, A., Aksoy, T. and Bahceci, M. (2011) Cryopreservation of Blastocysts Is the Most Feasible Strategy in Good Responder Patients. Fertility and Sterility, 96, 1121-1125.

[6]   Kovalevsky, G., Carney, S.M., Morrison, L.S., Boylan, C.F., Neithardt, A.B. and Feinberg, R.F. (2013) Should Embryos Developing to Blastocysts on Day 7 Be Cryopreserved and Trans-ferred: An Analysis of Pregnancy and Implantation Rates. Fertility and Sterility, 100, 1008-1012.

[7]   Levens, E.D., Whitcomb, B.W., Hennessy, S., James, A.N., Yauger, B.J. and Larsen, F.W. (2008) Blastocyst Development Rate Impacts Outcome in Cryopreserved Blastocyst Transfer Cycles. Fertility and Sterility, 90, 2138-2143.

[8]   Liebermann, J. (2009) Vitrification of Human Blastocysts: An Update. Reproductive Biomedicine Online, 19, 4328.

[9]   Majumdar, G., Majumdar, A., Verma, I.C. and Upadhyaya, K.C. (2017) Relationship between Morphology, Euploidy and Implantation Potential of Cleavage and Blastocyst Stage Embryos. Journal of Human Reproduction Science, 10, 142-150.

[10]   Alfarawati, S., Fragouli, E., Colls, P., Stevens, J., Gutierrez-Mateo, C., Schoolcraft, W.B., Katz-Jaffe, M.G. and Wells, D. (2011) The Relationship between Blastocyst Morphology, Chromosomal Abnormality, and Embryo Gender. Fertility and Sterility, 95, 520-524.