Induction of Anthracnose Disease Resistance on Chili Fruit by Treatment of Oligochitosan—Nanosilica Hybrid Material

Pham Dinh  Dzung; Le Thanh  Hung; Le Si  Ngoc; Hoang Dac  Hiet; Dang Van  Phu; Bui Van  Le; Nguyen Tien  Thang; Nguyen Ngoc  Duy; Nguyen Quoc  Hien

doi:10.4236/as.2017.810080

Pham Dinh Dzung^1,2, Le Thanh Hung¹, Le Si Ngoc¹, Hoang Dac Hiet¹, Bui Van Le³, Nguyen Tien Thang³, Dang Van Phu^2,4, Nguyen Ngoc Duy⁴, Nguyen Quoc Hien⁴^*

Abstract:

Oligochitosan (OC) with molecular weight Mw of 5000 g/mol was prepared by gamma Co-60 ray irradiation of chitosan solution. Nanosilica (nSiO₂) with the size of 10 - 30 nm was synthesized by calcination of acid treated rice husk. The mixture of 1% OC - 1% nSiO₂ was prepared by dispersion of nSiO₂ in OC solution. The morphology of nSiO₂ in the mixture of OC-nSiO₂ was measured from images of transmission electron microscopy (TEM). The effect of foliar application of the mixture of OC-nSiO₂ on the induction of resistance against anthracnose disease caused by Colletotrichum gloeosporioides fungus on chili fruits was investigated. Results indicated that foliar application of OC-nSiO₂ with the concentration of 60 mg/l - 60 mg/l was found to be as the optimal treatment that reduced the disease severity on chili fruits to 22.2% compared with 90.0% of the control. Thus, OC-nSiO₂ hybrid material could be considered as an effective biotic elicitor to prevent anthracnose disease infection for chili fruits. Furthermore, the prepared OC-nSiO₂ hybrid material can also be used as an environmentally friendly agrochemical product for sustainable development of agriculture.

Keywords: Oligochitosan, Nanosilica, Chili Fruit, Colletotrichum gloeosporioides, Anthracnose

Cite this paper: Dzung, P. , Hung, L. , Ngoc, L. , Hiet, H. , Le, B. , Thang, N. , Phu, D. , Duy, N. and Hien, N. (2017) Induction of Anthracnose Disease Resistance on Chili Fruit by Treatment of Oligochitosan—Nanosilica Hybrid Material. Agricultural Sciences, 8, 1105-1113. doi: 10.4236/as.2017.810080.

References

[1] Kim, K.W. and Thomas, R.L. (2007) Antioxidative Activity of Chitosans with Varying Molecular Weights. Food Chemistry, 101, 308-313.
https://doi.org/10.1016/j.foodchem.2006.01.038

[2] Zheng, L.Y. and Zhu, J.F. (2003) Study on Antimicrobial Activity of Chitosan with Different Molecular Weights. Carbohydrate Polymers, 54, 527-530.
https://doi.org/10.1016/j.carbpol.2003.07.009

[3] Chamnanmanoontham, N., Pongprayoon, W., Pichayangkura, R., Roytrakul, S. and Chadchawan, S. (2015) Chitosan Enhances Rice Seedling Growth via Gene Expression Network between Nucleus and Chloroplast. Plant Growth Regulation, 75, 101-114.
https://doi.org/10.1007/s10725-014-9935-7

[4] Dzung, N.A., Khanh, V.T.P. and Dzung, T.T. (2011) Research on Impact of Chitosan Oligomers on Biophysical Characteristics, Growth, Development and Drought Resistance of Coffee. Carbohydrate Polymers, 84, 751-755.
https://doi.org/10.1016/j.carbpol.2010.07.066

[5] Luan, L.Q., Nagasawa, N., Tamada, M. and Nakanishi, T.M. (2006) Enhancement of Plant Growth Activity of Irradiated Chitosan by Molecular Weight Fractionation. Radioisotopes, 55, 21-27.
https://doi.org/10.3769/radioisotopes.55.21

[6] Aziz, A., Trotel-Aziz, P., Dhuicq, L., Jeandet, P., Couderchet, M., et al. (2006) Chitosan Oligomers and Copper Sulfate Induce Grapevine Defense Reactions and Resistance to Gray Mold and Downy Mildew. Phytopathology, 96, 1188-1194.
https://doi.org/10.1094/PHYTO-96-1188

[7] Vasyukova, N.I., Zinov’eva, S.V., Il’inskaya, L.I., Perekhod, E.A., Chalenko, G.I., et al. (2001) Modulation of Plant Resistance to Diseases by Water-Soluble Chitosan. Applied Biochemistry and Microbiology, 37, 103-109.
https://doi.org/10.1023/A:1002865029994

[8] Yin, H., Zhao, X. and Du, Y. (2010) Oligochitosan: A Plant Disease Vaccine: A Review. Carbohydrate Polymers, 82, 1-8.
https://doi.org/10.1016/j.carbpol.2010.03.066

[9] Garg, A. and Balodi, R. (2014) Recent Trends in Agriculture: Vertical Farming and Organic Farming. Advances in Plants and Agriculture Research, 1, Article ID: 00023.
https://doi.org/10.15406/apar.2014.01.00023

[10] Stockdale, E.A., Lampkin, N.H., Hovi, M., Keatinge, R., Lennartsson, E.K.M., et al. (2001) Agronomic and Environmental Implications of Organic Farming Systems. Advances in Agronomy, 70, 263-327.
https://doi.org/10.1016/S0065-2113(01)70007-7

[11] Huang, S., Wang, L., Liu, L., Hou, Y. and Li, L. (2015) Nanotechnology in Agriculture, Livestock, and Aquaculture in China. A Review. Agronomy for Sustainable Development, 35, 369-400.
https://doi.org/10.1007/s13593-014-0274-x

[12] Taha, R.A. (2016) Nanotechnology and Its Application in Agriculture. Advances in Plants and Agriculture Research, 3, Article ID: 00089.
https://doi.org/10.15406/apar.2016.03.00089

[13] Kiirika, L.M., Stahl, F. and Wydra, K. (2013) Phenotypic and Molecular Characterization of Resistance Induction by Single and Combined Application of Chitosan and Silicon in Tomato against Ralstonia solanacearum. Physiological and Molecular Plant Pathology, 81, 1-12.
https://doi.org/10.1016/j.pmpp.2012.11.002

[14] Tiwari, R.K. (n.d.) Post Harvest Profile of Chili.
http://agmarknet.gov.in/Others/preface-chhilli.pdf

[15] Huq, A.S.M.A. and Arshad, F.M. (2010) Technical Efficiency of Chili Production. American Journal of Applied Sciences, 7, 185-190.
https://doi.org/10.3844/ajassp.2010.185.190

[16] Mahasuk, P., Khumpeng, N., Wasee, S., Taylor, P.W.J. and Mongkolporn, O. (2009) Inheritance of Resistance to Anthracnose (Colletotricum casici) at Seedling and Fruiting Stages in Chili Pepper (Capsicum spp.). Plant Breeding, 128, 701-706.
https://doi.org/10.1111/j.1439-0523.2008.01615.x

[17] Duy, N.N., Phu, D.V., Anh, N.T. and Hien, N.Q. (2011) Synergistic Degradation to Prepare Oligochitosan by γ-Irradiation of Chitosan Solution in the Presence of Hydrogen Peroxide. Radiation Physics and Chemistry, 80, 848-853.
https://doi.org/10.1016/j.radphyschem.2011.03.012

[18] Dzung, P.D, Ngoc, L.S., Thuy, N.N., Truc, L.T.M., Le, B.V., et al. (2016) Effect of Nanosilica from Rice Husk on the Growth Enhancement of Chili Plant (Casicum frutescens L.). Journal of Science and Technology, 54, 607-613.

[19] Tiraferri A., Maroni, P., Rodríguez, D.C. and Borkovec, M. (2014) Mechanism of Chitosan Adsorption on Silica from Aqueous Solutions. Langmuir, 30, 4980-4988.
https://doi.org/10.1021/la500680g

[20] Somapala, K.S., Weerahewa, H.L.D. and Thrikawala, S. (2016) Enhanced Resistance against Anthracnose Disease in Chili Pepper (Capsicum annuum L.) by Soil Application of Potassium. International Journal of Agriculture, Forestry and Plantation, 2, 35-39.
http://digital.lib.ou.ac.lk/docs/bitstream/701300122/1227/1/KLIAFP2_39.pdf

[21] Ahmad, A. and Ashraf, Y. (2016) In Vitro and In Vivo Management of Alternaria Leaf Spot of Brassica campestris L. Journal of Plant Pathology & Microbiology, 7, Article ID: 1000365.
https://doi.org/10.4172/2157-7471.1000365

[22] Das, S.N., Madhuprakash, J., Sarma, P.V.S.R.N., Purushotham, P., Suma, K., et al. (2015) Biotechnological Approaches for Field Applications of Chitooligosaccharides (COS) to Induce Innate Immunity in Plants. Critical Reviews in Biotechnology, 35, 29-43.
https://doi.org/10.4172/2157-7471.1000365

[23] Phu, D.V., Du, B.D., Tuan, L.N.A., Tam, H.V. and Hien, N.Q. (2017) Preparation and Foliar Application of Oligochitosan-Nanosilica on the Enhancement of Soybean Seed Yield. International Journal of Environment, Agriculture and Biotechnology, 2, 421-428.
https://doi.org/10.22161/ijeab/2.1.53

[24] Sumarni, W., Iswari, R.S., Marwoto, P. and Rahayu, E.F. (2016) Physical Characteristics of Chitosan-Silica Composite of Rice Husk Ash. IOP Conference Series: Materials Science and Engineering, 107, Article ID: 012039.
https://doi.org/10.1088/1757-899X/107/1/012039

[25] Ghormade, V., Deshpande, M.V. and Paknikar, K.M. (2011) Perspective for Nano-Biotechnology Enabled Protection and Nutrition of Plants. Biotechnology Advances, 29, 792-803.
https://doi.org/10.1016/j.biotechadv.2011.06.007

[26] Fruijtier-Pölloth, C. (2012) The Toxicological Mode of Action and the Safety of Synthetic Amorphous Silica-A Nanostructured Material. Toxicology, 294, 61-79.
https://doi.org/10.1016/j.tox.2012.02.001