AJPS  Vol.8 No.11 , October 2017
Decline in a Tilletia indica Teliospore Population in a Naturally Infested Arizona (USA) Wheat Field
Abstract: Following a 2004-outbreak of Karnal bunt in an Arizona wheat field where infection had not been detected in recent years, the population and viability of Tilletia indica teliospores in surface and subsurface layers of soil were monitored over a four-year period. The field was seeded with two parallel 1.8 m × 190 m strips of wheat, 11 m apart and the remainder planted in non-host barley. Thirty-eight 1.22 m2 wheat plots were harvested and seed examined for infection. Several times throughout each season soil samples were taken from each of 25 points throughout the field. Teliospores extracted from subsamples of each soil sample were examined to estimate the number of teliospores per gram soil and germination percentage. The average (SD) infection observed for each of the four consecutive seasons was 7.05 (4.6), 2.89 (5.5), 0, and 0.004% (0). The average number of teliospores recovered per gram of soil during the first sampling was 36 and 39 for the 0.5 cm deep (surface) and 5.5 cm deep (subsurface), respectively, with an average teliospore germination of 29%. By the last season, the average numbers of teliospores recovered were 5 and 2.5 per gram of soil, respectively, with an average germination of 1.0%.
Cite this paper: Peterson, G. and Kosta, K. (2017) Decline in a Tilletia indica Teliospore Population in a Naturally Infested Arizona (USA) Wheat Field. American Journal of Plant Sciences, 8, 2622-2634. doi: 10.4236/ajps.2017.811177.

[1]   Bonde, M.R, Peterson, G.L., Schaad, N.W. and Smilanick, J.L. (1997) Karnal Bunt of Wheat. Plant Disease, 81, 1370-1377.

[2]   Ykema, R.E., Floyd, J.P., Palm, M.E. and Peterson, G.L. (1996) First report of Karnal bunt of wheat in the United States. Plant Disease, 80, 1207.

[3]   Fuentes-Davila, G. (1998) Karnal Bunt of Wheat. In: Proceedings of Bunts and Smuts of Wheat: An International Symposium, North American Plant Protection Organization, Ottawa, 69-81.

[4]   Da Luz, W.C., Mendes, M.A.S., Ferreira, M.A.S.V. and Urben, A.F. (1993) Tilletia indica on Wheat in the South of the State of Rio Grande do Sul, Brazil and Measures for Eradication. Fitopatologica Brasileira, 18, S329.

[5]   Crous, P.W., Van Jaarsveld, A.B., Castlebury, L.A., Carris, L.M., Frederick, R.D. and Pretorius, Z.A. (2001) Karnal Bunt of Wheat Newly Reported from the African Continent. Plant Disease, 85, 561.

[6]   Anonymous (2006) Karnal Bunt. Title 7 Code of Federal Regulations, Pt 301, ed., 123-139.

[7]   Brennan, J.P. and Warham, E.J. (1989) Economic Losses from Karnal Bunt of Wheat in Mexico. Rpt. Joint CIMMYT-INIFAP Study, CIMMYT, Mexico.

[8]   Brennan, J.P., Warham, E.J., Byerlee, D. and Hernandez-Estrada, J. (1992) Evaluating the Economic Impact of Quality-Reducing, Seed-Borne Diseases: Lessons from Karnal Bunt of Wheat. Agricultural Economics, 6, 345-352.

[9]   Sansford, C.E., Baker, R.H.A., Brennan, J.P., Ewert, F., Gioli, B., Inman, A., Kinsella, A., Magnus, H.A., Miglietta, F., Murray, G.M., Porta-Puglia, A., Porter, J.R., Rafoss, T., Riccioni, L. and Thorne, F. (2008) The New Pest Risk Analysis for Tilletia indica, the Cause of Karnal Bunt of Wheat, Continues to Support the Quarantine Status of the Pathogen in Europe. Plant Pathology, 57, 603-611.

[10]   Regulated Areas (2005) Federal Register, 7 CFR Part 301.89-3(f). Vol. 70, No. 58.

[11]   Bonde, M.R., Berner, D.K., Nester, S.E., Peterson, G.L., Olsen, M.W., Cunfer, B.M. and Sim, T. (2004) Survival of Tilletia indica Teliospores in Different Soils. Plant Disease, 88, 316-324.

[12]   Rattan, G.S. and Aujla, S.S. (1990) Survival of Karnal-Bunt (Neovossia indica) Teliospores in Different Types of Soil at Different Depths. Indian Journal of Agricultural Science, 60, 616-618.

[13]   Babadoost, M., Mathre, D.E., Johnson, R.H. and Bonde, M.R. (2004) Survival of Teliospores of Tilletia indica in Soil. Plant Disease, 88, 56-62.

[14]   Bonde, M.R., Nester, S.E., Olsen, M.W. and Berner, D.K. (2004) Survival of Teliospores of Tilletia indica in Arizona Field Soils. Plant Disease, 88, 804-810.

[15]   Inman, A., Magnus, H.A., Riccioni, L., Hughes, K., Coates, M., Barnes, A., Barton, V., Sansford, C., Valvassori, M., Di Giambattista, G., Porta-Puglia, A., Razzaghian, J. and Peterson, G. (2008) Survival of Tilletia indica Teliospores under European Soil Conditions. Plant Pathology, 57, 290-300.

[16]   Dowell, F.E., Boratynski, T.N., Ykema, R.E., Dowdy, A.K. and Staten, R.T. (2002) Use of Optical Sorting to Detect Wheat Kernels Infected with Tilletia indica. Plant Disease, 86, 1011-1013.

[17]   United States Department of Agriculture, Animal and Plant Health Inspection Service (2007) Karnal Bunt Manual. USDA APHIS, 164 p.

[18]   Jhorar, O.P., Mavi, H.S., Sharma, I., Mah, G.S., Mathauda, S.S. and Singh, G. (1992) A Biometeorological Model for Forecasting Karnal Bunt Disease of Wheat. Plant Disease Research, 7, 204-209.

[19]   Stansbury, C.D. and McKirdy, S.J. (2001) Forecasting Climate Suitability for Karnal Bunt of Wheat: A Comparison of Two Meteorological Methods. Australasian Plant Pathology, 30, 1-12.

[20]   Workneh, F., Allen, T.W., Nash, G.H., Narasimhan, B., Srinivasan, R. and Rush, C.M. (2008) Rainfall and Temperature Distinguish between Karnal Bunt Positive and Negative Years in Wheat Fields in Texas. Phytopathology, 98, 95-100.

[21]   Melching, J.S., Bromfield, K.R. and Kingsolver, C.H. (1983) The Plant Pathogen Containment Facility at Frederick, Maryland. Plant Disease, 67, 717-722.

[22]   Rattan, G.S., Aujla, S.S. and Sharma, I. (1989) Estimation of Teliospores of T. indica from Field Soil. Plant Disease, 73, 804.

[23]   Babadoost, M. and Mathre, D.E. (1998) A Method for Extraction and Enumeration of Teliospores of Tilletia indica, T. controversa and T. barclayana in Soil. Plant Disease, 82, 1357-1361.

[24]   Peterson, G.L., Bonde, M.R. and Phillips, J.G. (2000) Size-Selective Sieving for Detecting Teliospores of Tilletia indica in Wheat Seed Samples. Plant Disease, 84, 999-1007.

[25]   Goates, B.J. and Peterson, G.L. (1999) Relationship between Soilborne and Seedborne Inoculum Density and the Incidence of Dwarf Bunt of Wheat. Plant Disease, 83, 819-824.

[26]   Peterson, G.L., Kosta, K.L., Glenn, D.L. and Phillips, J.G. (2008) Utilization of Soil Solarization for Eliminating Viable Tilletia indica Teliospores from Arizona Wheat Fields. Plant Disease, 92, 1604-1610.

[27]   Zhang, Y.G., Nearing, M.A., Liu, B.Y., Van Pelt, R.S., Stone, J.J., Wei H. and Scott, R.L. (2011) Comparative Rates of Wind versus Water Erosion from a Small Semiarid Watershed in Southern Arizona, USA.

[28]   United States Department of Agriculture, Animal and Plant Health Inspection Service (2015) National Karnal Bunt Survey. USDA APHIS, 164 p.

[29]   APHIS Amends Karnal Bunt (Tilletia Indica (Mitra) Mundkur) Regulated Areas in La Paz, Maricopa, and Pinal Counties, Arizona, and Removes Silage from the List of Regulated Articles (2017) (CFR) 301.89-3(f).