Nitrogen Fertilization and Inoculation Effects on Dry Bean

Ryan  Buetow; Grant H.  Mehring; Hans  Kandel; Burton  Johnson; Juan M.  Osorno

doi:10.4236/as.2017.810077

Ryan Buetow^*, Grant H. Mehring, Hans Kandel, Burton Johnson, Juan M. Osorno

Abstract:

Dry bean (Phaseolus vulgaris L.) has the ability to form symbiotic relationships with N₂-fixing bacteria. The research objectives were to evaluate yield and growth differences between dry bean cultivars from black, navy, and pinto market classes, with different N management combinations utilizing urea fertilizer, and two rhizobacteria inoculants. Research was conducted near Park River and Prosper, ND, during 2010, 2012, 2013. The experiment was a RCBD 3 × 2 × 3 factorial with four replicates with three dry bean cultivars, two N fertility levels, and two rhizobacteria inoculum sources plus a non-inoculated treatment. The pinto cultivar was also evaluated in 2014. Results showed significant cultivar main effect differences for nodules per plant, vigor, height, and 1000 seed weight. The pinto cultivar had significantly more nodules per root (19) compared with the black (13) and navy cultivars (9). There were not significant differences between inoculant treatments. The fertilizer main effect indicated significant differences for vigor and 1000 seed weight. No significant interactions were detected among factors. Addition of 56 kg N ha^-1 resulted in more vigorous plants, but had no effect on seed yield, and reduced 1000 seed weight at harvest at Park River in 2013. Root nodule number was higher with the inoculant treatments with 16.2 and 15.8 nodules per plant compared to the non-inoculated treatment with 10.7 nodules per plant at Park River in 2013. The pinto variety Lariat with an application of 56 kg ha^-1 had visually greener plants in 2013 and 2014, except Park River in 2013, but SPAD readings were not following the same trend. Inoculation and application of N fertilizer on dry bean may not be necessary if rhizobacteria are present and soil N levels are sufficiently high but future research on rates, timing, and N source and appropriate strains of Rhizobia inoculant on other pinto varieties is suggested.

Keywords: Dry Bean, Rhizobacteria, Nitrogen, Legume

Cite this paper: Buetow, R. , Mehring, G. , Kandel, H. , Johnson, B. and Osorno, J. (2017) Nitrogen Fertilization and Inoculation Effects on Dry Bean. Agricultural Sciences, 8, 1065-1081. doi: 10.4236/as.2017.810077.

References

[1] United States Department of Agriculture (USDA) (2017) Quick Stats 2.0. U.S. Department of Agriculture, National Agricultural Statistics Service, Washington DC.
https://quickstats.nass.usda.gov/

[2] Franzen, D. (2006) Fertilizing Pinto, Navy, and Other Dry Edible Beans. NDSU Ext. Serv. SF 720. North Dakota Agric. Exp. Station, Fargo, ND.

[3] Franzen, D. (2013) Dry Bean Fertility. In: Dry Bean Production Guide, Bull. A-1133, NDSU Ext. Serv., Fargo, 18-23.

[4] Knodel, J., Luecke, J., Beauzay, P., Franzen, D., Kandel, H., Markell, S., Osorno, J.M. and Zollinger, R. (2011) 2010 Dry Bean Grower Survey of Pest Problems and Pesticide Use in Minnesota and North Dakota. NDSU Ext. Serv. Bull. No. E-1522.

[5] Fageria, N.K. and Santos, A.B. (2008) Yield Physiology of Dry Bean. Journal of Plant Nutrition, 31, 983-1004.
https://doi.org/10.1080/01904160802096815

[6] Stevens, B. and Belden, K. (2005) Nutrient Management Guidelines for Dry Beans. Reg. Bull. B-1016R. Coop. Ext. Serv. and Coll. of Ag. Uni. of Wyoming, Laramie.

[7] Wetzel, R.G. and Likens, G.E. (1991) Inorganic Nutrients: Nitrogen, Phosphorus, and Other Nutrients. In: Wetzel, R.G. and Likens, G.E., Eds., Limnological Analyses, Springer-Verlag, New York.
https://doi.org/10.1007/978-1-4757-4098-1_7

[8] Havlin, J.L., Beaton, J.D., Tisdale, S.L. and Nelson, W.L. (2005) Nitrogen. In: Yarnell, D., Yehle, K., Kiefer, J. and Carnis, M., Eds., Soil Fertility and Fertilizers, Pearson Education Inc., Upper Saddle River, 97-112.

[9] Schoenau, J. (2016) Fertility Requirements and Contributions of Pulse Crops.
http://proof.saskpulse.com/files/general/160205_Schoenau_2016_Sask_Pulse_Tour_fertility_
presentation.pdf

[10] Edje, O., Mughogho, L. and Ayonoadu, U. (1975) Responses of Dry Beans to Varying Nitrogen Levels. Agronomy Journal, 67, 251-255.
https://doi.org/10.2134/agronj1975.00021962006700020020x

[11] O’Leary, M., Rehm, G. and Schmitt, M. (2002) Understanding Nitrogen in Soils. Univ. of Minnesota, Coop Ext. Serv.
http://www.extension.umn.edu/agriculture/nutrient-management/nitrogen/understanding-nitrogen-in-soils/

[12] Brady, N. and Weil, R. (2010) Nutrient Cycles and Soil Fertility. In: Anthony, V.R., Ed., Elements of the Nature and Properties of Soils, 3rd Edition, Pearson Education Inc, Upper Saddle River, NJ, 396-420.

[13] Graham, P.H. (1978) Some Problems and Potentials of Field Beans Phaseolus vulgaris in Latin America. Field Crops Research, 1, 295-317.

[14] Farid, M. and Navabi, A. (2015) N2 Fixation Ability of Different Dry Bean Genotypes. Canadian Journal of Plant Science, 95, 1243-1257.
https://doi.org/10.4141/CJPS-2015-084

[15] Hungria, M. and Vargas, M.A.T. (2000) Environmental Factors Affecting N2 Fixation in Grain Legumes in the Tropics, with an Emphasis on Brazil. Field Crops Research, 5, 151-164.

[16] Graham, P.H. (1981) Some Problems of Nodulation and Symbiotic Nitrogen Fixation in Phaseolus vulgaris L.: A Review. Field Crops Research, 4, 93-112.

[17] Kipe-Nolt, J.A., Vargas, H. and Giller, K.E. (1993) Nitrogen Fixation in Breeding Lines of Phaseolus vulgaris L. Plant Soil, 152, 103-106.
https://doi.org/10.1007/BF00016338

[18] Eckert, F.R., Kandel, H.J., Johnson, B.L., Rojas-Cifuentes, G.A., Deplazes, C., Vander Wal, A.J. and Osorno, J.M. (2011) Row Spacing and Nitrogen Effects on Upright Pinto Bean Cultivars under Direct Harvest Conditions. Agronomy Journal, 103, 1314-1320.
https://doi.org/10.2134/agronj2010.0438

[19] Kellman, A.W., Hill, G.D. and McKenzie, B.A. (2005) Variability in Nodulation of Phaseolusvulgaris L. with Different Rhizobial Strains. Agron. New Zealand, 35, 57-65.

[20] Fageria, N.K., Melo, L.C., Ferreira, E.P.B., Oliveira, J.P. and Knupp, A.M. (2014) Dry matter, Grain Yield, and Yield Components of Dry Bean as Influenced by Nitrogen Fertilization and Rhizobia. Communications in Soil Science and Plant Analysis, 45, 111-125.
https://doi.org/10.1080/00103624.2013.848877

[21] Weiser, G.C., Grafton, K.F. and Berryhill, D.L. (1985) Nodulation of Dry Beans by Commercial and Indigenous Strains of Rhizobium phaseoli. Agronomy Journal, 77, 856-859.
https://doi.org/10.2134/agronj1985.00021962007700060007x

[22] Henson, B. and Halvorson, M. (2004) Dry Bean Response to Nitrogen Fertilizer.
https://www.ag.ndsu.edu/archive/carringt/agronomy/Research/Fertility/04%20DEB%20Response%
20to%20N.pdf

[23] Nanjareddy, K., Blanco, L., Arthikala, M.K., Affantrange, X.A., Sanchez, F. and Lara, M. (2014) Nitrate Regulates Rhizobial and Mycorrhizal Symbiosis in Common Bean (Phaseolus vulgaris L.). Journal of Integrative Plant Biology, 56, 281-298.
https://doi.org/10.1111/jipb.12156

[24] Cassman, K.G., Dobermann, A. and Walters, D.T. (2002) Agroecosystems, Nitrogen-Use Efficiency, and Nitrogen Management. AMBIO, 31, 132-140.
https://doi.org/10.1579/0044-7447-31.2.132

[25] Davis, J.G. and Brick, M.A. (2009) Fertilizing Dry Beans. Colorado State Univ. Ext. Fact Sheet No. 0539.

[26] Bullock, D.G. and Anderson, D.S. (2008) Evaluation of the Minolta SPAD-502 Chlorophyll Meter for Nitrogen Management in Corn. Journal of Plant Nutrition, 21, 741-755.
https://doi.org/10.1080/01904169809365439

[27] Fritschi, F.B. and Ray, J.D. (2007) Soybean Leaf Nitrogen, Chlorophyll Content, and Chlorophyll a/b Ratio. Photosynthetica, 45, 92-98.
https://doi.org/10.1007/s11099-007-0014-4

[28] Osorno, J.M., Grafton, K.F., Rasmussen, J.B., Rojas-Cifuentes, G.A., Gelin, R. and Vander-Wal, A.J. (2009) Release of “Eclipse” Black Bean. Bean Improvement Cooperative. Annual Report, Vol. 52, 160-161.

[29] Osorno, J.M., Grafton, K.F., Rojas-Cifuentes, G.A., Gelin, R. and Vander Wal, A. (2010) Registration of “Lariat” and “Stampede” Pinto Beans. Journal of Plant Registrations, 4, 5-11.
https://doi.org/10.3198/jpr2009.03.0143crc

[30] Kandel, H. (2013) Drybean Production Guide. Bull. No. A-1133 (Revised). NDSU Ext. Serv., Fargo.

[31] United States Department of Agriculture (USDA) (2017).
http://websoilsurvey.nrcs.usda.gov/app/WebSoilSurvey.aspx

[32] LeBaron, M.J. (1974) Developmental Stages of the Common Bean Plant; A Description. Univ. of Idaho, Coop Ext. Serv. No. 228.

[33] Penn State College of Agricultural Sciences (2012) Common Bean Shovelomics.
http://plantscience.psu.edu/research/labs/roots/methods/field/shovelomics/intensive-bean-crown-phenotyping

[34] NDAWN. North Dakota Agricultural Weather Network (2017) North Dakota State Univ., Fargo.
http://ndawn.ndsu.nodak.edu