AS  Vol.8 No.9 , September 2017
Effects of Feeding Combinations of Soybean and Linseed Oils on Productive Performance and Milk Fatty Acid Profile in Grazing Dairy Cows
Abstract: Thirty-six grazing dairy cows were used to determine the effect of combinations of soybean (SO), and linseed (LO) oils on milk production, composition and milk fatty acid (FA) profile. Treatments were a basal control diet (56% pasture, 44% concentrate) or the control diet supplemented with oils at 4% of estimated total dry matter (DM) intake. Oils were manually mixed to the concentrate in pure forms (SO100 or LO100) or in blends (%w/w) at SO75 - LO25, SO50 - LO50 and SO25 - LO75. Concentrate and oils were thoroughly consumed. Pasture intake (kg DM/cow·day) was 9.27 in control and decreased (p < 0.05) in SO25 - LO75 (8.09) and LO100 (8.98). Total DM intake (kg/cow·day) in control (16.47) increased (p < 0.05) to 17.04 in SO100 and 17.20 in SO75. Yield of fat corrected milk (4% FCM) averaged 20.73 kg in control resulting higher in SO75 (23.73 kg). Milk fat content (g/100g) in control averaged 3.40 and decreased to 2.79 in SO50-LO50 and to 3.06 in SO25 - LO75 treatments. Milk protein content was not affected and milk protein yield increased in SO100 (11%) and SO75 - LO25 (21%) over Control (0.729 kg/cow·day). Milk basal (Control) content (g/100g FA) of C12:0 (2.58), C14:0 (10.21) and C16:0 (25.69) was reduced (p < 0.05) to 1.64, 6.82 and 19.70 respectively in oil supplemented cows. Basal content of C12:0 to C16:0 averaged 38.48 g/100g FA and decreased (27.4%) after oil intake. Basal trans-10 C18:1 (0.46 g/100g FA) increased (p < 0.01) in SO100 (1.48) and SO50-LO50 (1.80). Basal level (g/100g FA) of vaccenic acid (trans-11 C18:1, VA) averaged 3.49 and increased (135%) after oil intake with maximum values observed in LO100 (8.17) and SO50 - LO50 (9.20). Rumenic acid (cis-9, trans-11 C18:2, RA) level (g/100g FA) in milk from Control cows (1.56) increased (p < 0.05) to 3.03 (SO100), 3.21 (SO75 - LO25), 3.24 (SO50 - LO50), 2.33 (SO25 - LO75) and 2.96 (LO100). Results obtained confirmed a great milk fat plasticity in response to PUFA feeding in grazing dairy cows which constitutes a very effective and easy tool in order to improve the healthy value of milk with a potential benefit to the consumer’s health. A net or conclusive response pattern over parameters that improve the healthy value of milk to soybean and linseed oils and their blends was not clearly detected. Taken together, the results suggest some advantage for the SO75:LO25 blend considering the relative costs of both oils, the positive effects on milk, fat and protein yields, the lower hypercholesterolemic FA content of milk and the increase in VA and RA content while maintaining a healthy n - 6/n - 3 ratio and very low levels of the detrimental trans-9 C18:1 and trans-10 C18:1 FA.
Cite this paper: Antonacci, L. , Gagliostro, G. , Virginia Cano, A. and Adrián Bernal, C. (2017) Effects of Feeding Combinations of Soybean and Linseed Oils on Productive Performance and Milk Fatty Acid Profile in Grazing Dairy Cows. Agricultural Sciences, 8, 984-1002. doi: 10.4236/as.2017.89072.

[1]   Ulbritch, T.L. and Southgate, D.A.T. (1991) Coronary Heart Disease: Seven Dietary Factors. Lancet, 338, 985-992.

[2]   Turpeinen, A.M., Mutanen, M., Aro, A., Salminen, I., Basu, S., Palmquist, D.L. and Griinari, J.M. (2002) Bioconversion of Vaccenic Acid to Conjugated Linoleic Acid in Humans. American Journal of Clininical Nutrition, 76, 504-510.

[3]   Chilliard Y., Ferlay A., Mansbridge R.M. and Doreau M. (2000) Ruminant Milk Fat Plasticity: Nutritional Control of Saturated, Polyunsaturated, Trans and Conjugated Fatty Acids. Annales de Zootechnie, 49, 181-205.

[4]   Gagliostro, G.A. (2004) Nutritional Control of Conjugated Linoleic Acid (CLA) Content in Milk and Its Presence in Functional Natural Foods. 2. Production of High Cow’s Milk CLA. Revista Argentina de Producción Animal, 24, 137-163.

[5]   Harvatine, K. and Bauman, D.E. (2006) SREBP1 and Thyroid Hormone Responsive Spot 14 (S14) Are Involved in the Regulation of Bovine Mammary Lipid Synthesis during Diet-Induced Fat Depression and Treatment with CLA. Journal of Nutrition, 136, 2468-2474.

[6]   Chilliard Y. and Ferlay, A. (2004) Dietary Lipids and Forages Interactions on Cow and Goat Milk Fatty Acid Composition and Sensory Properties. Reproduction Nutrition Developement, 44, 467-492.

[7]   Rego, O.A., Alves, S.P., Antunes, L.M.S., Rosa, H.J.D., Alfaia, C.F.M., Prates, J.A.M., Cabrita, A.R.J., Fonseca, A.J.M. and Bessa, R.J.B. (2009) Rumen Biohydrogenation-Derived Fatty Acids in Milk Fat from Grazingdairy Cows Supplemented with Rapeseed, Sunflower, or Linseed Oils. Journal of Dairy Science, 92, 4530-4540.

[8]   Castillo Vargas, J.A. (2012) Kinetics of in Vitro Biohydrogenation of Polyunsaturated Fatty Acids in Ruminal Fluid. Master Science Thesis in Animal Production, Universidad Nacional de Colombia. Facultad de Medicina Veterinaria y de Zootecnia, Departamento de Producción Animal. Bogotá, Colombia.

[9]   Hartoof, C.G., Noble, R.C. and Moore, J.H. (1973) Factors Influencing the Extent of Biohydrogenation of Linoleic Acid by Rumen Micro-Organisms in Vitro. Journal of Science Food Agriculture, 24, 961-970.

[10]   Agazzi, A., Bayourthe, C., Nicot, M.C., Troegeler-Meynadier, A., Moncoulon, R. and Enjanbert, F. (2004) In Situ Ruminal Biohydrogenation of Fatty Acids from Extruded Soybeans: Effects of Dietary Adaptation and of Mixing with Lecithin or Wheat Straw. Animal Feed Science and Technology, 117, 165-175.

[11]   Gómez-Cortés, P., Frutos, P., Mantecón, A.R., Juárez, M., De la Fuente, M.A. and Hervás, G. (2008) Milk Production, Conjugated Linoleic Acid Content, and in Vitro Ruminal Fermentation in Response to High Levels of Soybean Oil in Dairy Ewe Diet. Journal of Dairy Science, 91,1560-1569.

[12]   Roy, A., Chardigny, J.M., Bauchart, D., Ferlay, A., Lorenz, S., Durand, D., Duffart, D., Faulconnier, Y., Sébédio, J.L. and Chilliard, Y. (2007) Butters Rich Either in Trans-10-C18:1 or in Trans-11-C18:1 plus cis-9-trans11 CLA Differentially Affect Plasma Lipids and Aortic Fatty Streak in Experimental Atherosclerosis in Rabbits. Animal, 1, 467-476.

[13]   Gagliostro, G.A. (2004) Nutritional Control of Conjugated Linoleic Acid (CLA) Content in Milk and Its Presence in Functional Natural Foods. 3. Production of High Milk CLA through Strategic Supplementation of the Goat. Revista Argentina de Producción Animal, 24, 165-185.

[14]   Gómez-Cortés, P. (2010) Effect of Supplementation of the Ovine Diet with Different Lipid Sources on the Fatty Acid Profile of Milk. Universidad Complutense de Madrid. Facultad de Ciencias Químicas. Departamento de Química Física I.

[15]   Gagliostro, G.A., Patino, E.M., Sanchez Negrette, M., Sager, G., Castelli, L., Antonacci, L.E., Raco, F., Gallello, L., Rodríguez, M.A., Canameras, C., Zampatti, M.L. and Bernal, C. (2015) Milk Fatty Acid Profile from Grazing Buffaloes Fed a Blend of Soybean and Linseed Oils. Arquivo Brasileiro de Medicina Veteterinaria e Zootecnia, 67, 927-934.

[16]   Masson, L., Alfaro, T., Camilo, C., Carvalho, A., Illesca, P., Torres, R., Tavares do Carmo, M., Mancini-Filho, J. and Bernal, C. (2015) Fatty Acid Composition of Soybean/Sunflower Mix Oil, Fish Oil and Butterfat Applying the AOCS Ce 1j-07 Method with a Modified Temperature Program. Grasas y Aceites, 66, e064.

[17]   Komareck, A.R., Robertson, J.B. and Van Soest, P.J. (1994) Comparison of the Filter Bag Technique to Conventional Filtration in the Van Soest NDF Analysis of 21 Feeds. In: Fahey, G.C., Ed., Proceedings of National Conference on Forage Quality, Evaluation and Utilization, Nebraska University, Lincoln, 2.

[18]   Komareck, A.R. (1993) An Improved Filtering Technique for the Analysis of Neutraldetergent Fiber and Acid Detergent Fiber Utilizing the Filter Bag Technique. Journal of Animal Science, 71, 824-829.

[19]   Horneck, D.A. and Miller, R.O. (1998) Determination of Total Nitrogen in Plant Tissue. In: Kalra, Y.P., Eds., Handbook of Reference Methods for Plant Analysis, Soil and Plant Analysis Council, Inc. CRC Press, Boca Raton, 75-83.

[20]   Morris, L.D. (1948) Quantitative Determination of Carbohydrates with Dreywood’ Santhrone Reagent. Science, 107, 254-255.

[21]   AOAC (2006) Official Methods of Analysis of the Association of Official Agricultural Chemists. 18th Edition, AOAC International, Gaithersburg.

[22]   McRae, J.C. and Armstrong, D.G. (1968) Enzyme Method for Determination of Alpha-Linked Glucose Polymers in Biological Materials. Journal of the Science of Food and Agriculture, 19, 578-581.

[23]   Meijs, J.A.C., Walters, R.J.K. and Keen, A. (1982) Sward Methods. In: Herbage Intake Handbook, British Grassland Society, 11-36.

[24]   SAS Institute Inc. (2002-2010) SAS/STAT User’s Guide. Cary.

[25]   Minson, D.J. (1990) Forage in Ruminant Nutrition. Academic Press Inc., San Diego, 482 p.

[26]   Leaver, J.D. (1985) Herbage Intake Handbook. British Grassland Society, Hurley, 143 p.

[27]   Verité, R. and Journet, M. (1970) Influence de la teneur en eau et de la deshydratation de l’herbe sur sa valeur alimentaire pour les vaches laitières. [Influence of Water Content and Dehydration of the Forage on Its Dietary Value for Dairy Cows]. Annales de Zootechnie, 10, 269-277.

[28]   Glasser, F., Ferlay, A. and Chilliard, Y. (2008) Oilseed Lipid Supplements and Fatty Acid Composition of Cow Milk: A Meta-Analysis. Journal of Dairy Science, 91, 4687-4703.

[29]   Martínez, M.G. (2010) Modulation of the Fatty Acid Composition of Bovine and Caprine Milk through Supplementation with Soybean and Fish Oil. Master Science Thesis, Faculty of Agrarian Sciences, National University of Mar de Plata, Argentina, 130 p.

[30]   Flowers, G., Ibrahim, S.A. and AbuGhazaleh, A.A. (2008) Milk Fatty Acid Composition of Grazing Dairy Cows When Supplemented with Linseed Oil. Journal of Dairy Science, 90, 3786-3801.

[31]   Martin, C., Rouel, J., Jouany, J.P., Doreau, M. and Chilliard, Y. (2008) Methane Output and Diet Digestibility in Response to Feeding Dairy Cows Crude Linseed, Extruded Linseed, or Linseed Oil. Journal of Animal Science, 86, 2642-2650.

[32]   Pires, J.A.A., Pescara, J.B., Brickner, A.E., Silva del Rio, N., Cunha, A.P. and Grummer, R.R. (2008) Effects of Abomasal Infusion of Linseed Oil on Responses to Glucose and Insulin in Holstein Cows. Dairy Science, 91, 1378-1390.

[33]   Loor, J.J., Ferlay, A., Ollier, A., Doreau, M. and Chilliard, Y. (2005) Relationship among Trans and Conjugated Fatty Acids and Bovine Milk Fat Yield Due to Dietary Concentrate and Lindseed Oil. Journal of Dairy Science, 88, 726-740.

[34]   Bu, D.P., Wang, J.G., Dhiman, T.R. and Liu, S.J. (2007) Effectiveness of Oils Rich in Linoleic and Linolenic Acids to Enhance Conjugated Linoleic Acid in Milk from Dairy Cows. Journal of Dairy Science, 90, 998-1007.

[35]   Morand-Fehr, P., Chilliard, Y. and Bas, P. (1986) Repercusions de l’Apport de Matieres Grasses dans la Ration sur la Production et la Composition du Lait de Ruminant. [Impact of Including Fats in the Ration on Yield and Composition of Ruminant Milk]. Institut National de la Recherche Agronomique, 64, 59-72.

[36]   Dhiman, T.R., Satter, L.D., Pariza, M.W., Galli, M.P., Albright, K. and Tolosa, M.X. (2000) Conjugated Linoleic Acid (CLA) Content of Milk from Cows Offered Diets Rich in Linoleic and Linolenic Acid. Journal of Dairy Science, 83, 1016-1027.

[37]   Alzahal, O., Odongo, N.E., Mutsvanqwa, T., Or-Rashid, M.M., Duffield, T.F., Baqq, R., Dick, P., Vessie, G. and McBride, B.W. (2008) Effects of Monensin and Dietary Soybean Oil on Milk Fat Percentage and Milk Fatty Acid Profile in Lactating Dairy Cows. Journal of Dairy Science, 91, 1166-1174.

[38]   Huang, Y., Schoonmaker, J.P., Bradford, B.J. and Beitz, D.C. (2008) Response of Milk Fatty Acid Composition to Dietary Supplementation of Soy Oil, Conjugated Linoleic Acid, or Both. Journal of Dairy Science, 91, 260-270.

[39]   Gagliostro, G.A. and Chilliard, Y. (1992) Use of Protected Lipids in Dairy Cow Nutrition. I. Effects on the Production and Composition of Milk, and on Intake of Dry Matter and Energy. Revista Argentina de Producción Animal, 12, 1-15.

[40]   Schroeder, G.F., Gagliostro, G.A., Bargo, F., Delahoy, J.E. and Muller, L.D. (2004) Effects of Fat Supplementation on Milk Production and Composition by Dairy Cows on Pasture: A Review. Livestock Production Science, 86, 1-18.

[41]   Piperova, L.L., Teter, B.B., Bruckental, I., Sampugna, J., Mills, S.E., Yurawecz, M.P., Fritsche, J., Ju, K. and Erdman, R.A. (2000) Mammary Lipogenic Enzyme Activity, Trans Fatty Acids and Conjugated Fatty Acids Are Altered in Lactating Dairy Cows Fed a Milk-Fat Depressing Diet. Journal of Nutrition, 130, 2568-2574.

[42]   Palmquist, D.L., Beaulieu, A.D. and Barbano, D.M. (1993) Feed and Animal Factors Influencing Milk Fat Composition. Journal of Dairy Science, 76,1753-1771.

[43]   Bargo, F., Muller. L.D., Kolver, E.S. and Delahoy, J.E. (2003) Invited Review: Production and Digestion of Supplemented Dairy Cows on Pasture. Journal of Dairy Science, 86, 1-42.

[44]   AbuGhazaleh, A.A., Schingoethe, D.J., Hippen, A.R., Kalscheur, K.F. and Whitlock, A. (2002) Fatty Acid Profiles of Milk and Rumen Digesta from Cows Fed Fish Oil, Extruded Soybeans or Their Blend. Journal of Dairy Science, 85, 2266-2276.

[45]   AbuGhazaleh, A.A., Schingoethe, D.J., Hippen, A.R.K.F. and Whitlock, A. (2002) Feeding Fish Meal and Extruded Soybeans Enhances the Conjugated Linoleic Acid (CLA) Content of Milk. Journal of Dairy Science, 85, 624-631.

[46]   Angulo, J. (2012) Effects of Polyunsaturated Fatty Acids from Plant Oils and Algae on Milk Fat Yield and Composition Are Associated with Mammary Lipogenic and SREBF1 Gene Expression. Tesis, Universidad de Antioquia, Facultad de Ciencias Agrarias, Colombia.

[47]   Gagliostro, G.A. and Chilliard, Y. (1992) Bibliographic Review. Use of Lipids Protected in Nutrition of Dairy Cows. II. Effects on Plasma Concentration of Metabolites and Hormones, Mobilization of Body Lipids and Metabolic Activity of Adipose Tissue. Revista Argentina de Producción Animal, 12, 17-32.

[48]   Sutton, J.D., Knight, R., Mcallan, A.B. and Smith, R.H. (1983) Digestion and Synthesis in the Rumen of Sheep Given Diets Supplemented with Free and Protected Oils. British Journal of Nutrition, 49, 419-432.

[49]   Gagliostro, G.A., Garciarena, D.A., Rodriguez, M.A. and Antonacci, L.E. (2017) Feeding Polyunsaturated Supplements to Grazing Dairy Cows Improve the Healthy Value of Milk Fatty Acids. Agricultural Sciences, 8, 759-782.

[50]   Doreau, M. and Chilliard, Y. (1997) Effects of Ruminal or Postruminal Fish Oil Supplementation on Intake and Digestion in Dairy Cows. Reproduction Nutrition Development, 37, 113-124.

[51]   Ueda, K., Ferlay, A., Chabrot, J., Loor, J.J., Chilliard, Y. and Doreau, M. (2003) Effect of Linseed Oil Supplementation on Ruminal Digestion in Dairy Cows Fed Diets with Different Forage: Concentrate Ratios. Journal of Dairy Science, 86, 3999-4007.

[52]   Gagliostro, G.A., Chilliard, Y. and Davicco, M.J. (1991) Duodenal Rapeseed Infusion in Early and Midlactation Cows. 3. Plasma Hormones and Mammary Uptake of Metabolites. Journal of Dairy Science, 74, 1893-1903.

[53]   Christie, W.W. (1981) The Effects of Diet and Other Factors on the Lipid Composition of Ruminant Tissues and Milk. In: Christie, W.W., Ed., Lipid Metabolism of Ruminant Animals, Pergamon Press, Oxford, 193-226.

[54]   Storry, J.E. (1981) The Effect of Dietary Fat on Milk Composition. In: Haresing, W., Ed., Recent Advances in Animal Nutrition, Butterworths, London, 3-33.

[55]   Antonacci, L.E., Rodríguez, A., Castelli, L., Zampatti, M., Castaneda, R., Ceaglio, J. and Gagliostro, G.A. (2013) Supplementation with a Blend of Vegetable Oils and the Fatty Acid Profile of Bovine Milk. Revista Argentina de Producción Animal, 33.

[56]   Griinari, J.M. and Bauman, D.E. (2006) Milk Fat Depression: Concepts, Mechanisms and Management Applications. In: Sjersen, K., Hvelplund, T. and Nielsen, M.O., Eds., Ruminant Physiology: Digestion, Metabolism and Impact of Nutrition on Gene Expression, Immunology and Stress, Wageningen Academic Publishers, Holand, 389-417.

[57]   Ward, A.T., Wittenberg, K.M. and Przybylski, R. (2002) Bovine Milk Fatty Acid Profiles Produced by Feeding Diets Containing Solin, Flax and Canola. Journal of Dairy Science, 85, 1191-1196.

[58]   Cruz-Hernandez, C., Kramer, J.K.G., Kennelly, J.J., Glimm, D.R., Sorensen, B.M., Okine, E.K., Goonewardene, L.A. and Weselake, R.J. (2007) Evaluating the Conjugated Linoleic Acid and Trans 18:1 Isomers in Milk Fat of Dairy Cows Fed Increasing Amounts of Sunflower Oil and a Constant Level of Fish Oil. Journal of Dairy Science, 90, 3786-3801.

[59]   Chilliard, Y., Martin, C., Rouel, J. and Doreau, M. (2009) Milk Fatty Acids in Dairy Cows Fed Whole Crude Linseed, Extruded Linseed, or Linseed Oil, and Their Relationship with Methane Output. Journal of Dairy Science, 92, 5199-5211.

[60]   Hurtaud, C., Faucon, F., Couvreur, S. and Peyraud, J.L. (2010) Linear Relationship between Increasing Amounts of Extruded Linseed in Dairy Cow Diet and Milk Fatty Acid Composition and Butter Properties. Journal of Dairy Science, 93, 1429-1443.

[61]   Rego, O.A., Rosa, H.J.D., Portugal, P., Cordeiro, R., Borba, A.E.S., Vouzela, C.M. and Bessa, R.J.B. (2005) The Effects of Supplementation with Sunflower and Soybeans Oils on the Fatty Acid Profile of Milk Fat from Grazing Dairy Cows. Animal Research, 54, 17-24.

[62]   Schroeder, G.F. and Gagliostro, G.A. (2007) Partial Replacement of Corn Grain with Calcium Salts of Fatty Acid in the Concentrate Fed to Grazing Primiparous and Multiparous Dairy Cows. New Zealand Journal of Agricultural Research, 50, 437-449.