AiM  Vol.7 No.8 , August 2017
Soil Urease Activity of Sundarban Mangrove Ecosystem, India
Abstract: Vertical occurrence of soil urease activity along with ammonia content from three distinct regions viz. Deep forest region (No tidal action and wave attack occurs as it is furthest from river shore and it contains maximum content of organic carbon and minimum soil salinity and silicate concentration. In this zone plenty of pneumatophores, below ground root and dense vegetation are found), Rooted region (It is situated in between Deep forest region and Un-rooted region. This region contains only pneumatophores but it is devoid of long roots and vegetations. It faces wave attack and tidal action less than that of Un-rooted region) and Un-rooted region (It is closest to river shore and faces maximum wave attack and tidal action; it contains minimum organic carbon but maximum soil salinity and silicate concentration. This zone is totally devoid of any roots, pneumatophores and vegetations) of Sundarban mangrove forest ecosystem, India revealed an interesting explanation. Soil urease activity showed a decreasing pattern with increase in depth from the deep forest region of the Sundarban forest ecosystem. Soil urease activity was found to be more sensitive to soil temperature and pH rather than soil salinity. This ensured that soil urease along with the microbes present in the Sundarban forest ecosystem are more tolerant to fluctuation in salinity than that of temperature. Soil ammonia concentration was found to be directly governed by the soil urease activity [The regression equation is Ammonia in soil = -1.64 + 0.0402 Urease Activity (R-Sq = 62.9%, P < 0.001, n = 41)].
Cite this paper: Das, S. , Ganguly, D. , Mukherjee, A. , Chakraborty, S. and De, T. (2017) Soil Urease Activity of Sundarban Mangrove Ecosystem, India. Advances in Microbiology, 7, 617-632. doi: 10.4236/aim.2017.78048.

[1]   Twilley, R.R., Chem, R.H. and Hargis, T. (1992) Carbon Sinks in Mangroves and Their Implications to Carbon Budget to Tropical Coastal Ecosystem. Water, Air & Soil Pollution, 54, 265-288.

[2]   Gattuso, P., Prakignoulle, M. and Wollast, R. (1998) Carbon and Carbonate Metabolism in Coastal Aquatic Ecosystems. Annual Review of Ecology, Evolution, and Systematics, 29, 405-434.

[3]   Alongi, D.M. (2009) The Energetics of Mangrove Forest. Springer, Dordrecht, 11-24.

[4]   FAO (2004) Status and Trends in Mamgrove Area Extent Worldwide. Forest Resource Assessment Working Paper, 063, 287.

[5]   Lovelock, C.E., Feller, I.C., Mc Kee, K.L. and Thompson, R. (2005) Variation in Mangrove Forest Structure and Sediment Characteristics in Bolas del Toro, Panama. Caribbean Journal of Science, 41, 456-464.

[6]   Sanders, C.J., Smoak, J.M., Sathy, N.A., Sanders, L.M. and Patchineelam, S.R. (2010) Organic Carbon Burial in a Mangrove Forest, Margin and Intertidal Mud Flat. Estuarine, Coastal and Shelf Science, 90, 168-172.

[7]   Sanders, C.J., Smoak, J.M., Waters, M.N., Sanders, L.M., Brandini, N. and Patchineelam, S.R. (2012) Organic Matter Content and Particle Size Modifications in Mangrove Sediments as Responses to Sea Level Rise. Marine Environmental Research, 77, 150-155.

[8]   Pinck, L.A. and Allison, F.E. (1961) Adsorption and Release of Urease by and from Clay Minerals. Soil Science, 91, 183-188.

[9]   McLaren, A.D. (1963) Enzyme Activity in Soils Sterilized by Ionising Radiation and Some Comments on Microenvironments in Nature. In: Gibbons, N.E., Ed., Recent Progress in Microbiology, University of Toronto Press, Toronto, Vol. 8, 221-229.

[10]   Dinesh, R., Chaudhuri, S.G., Ganeshamurthy, A.N. and Pramanik, S.C. (2004) Biochemical Properties of Soils of Undisturbed and Disturbed Mangrove Forests of South Andaman (India). Wetlands Ecology and Management, 12, 309-320.

[11]   Yang, Q., Tam, N.F.Y., Wong, Y.S., Luan, T.G., Su, W.S., Lan, C.Y., Shin, P.K.S. and Cheung, S.G. (2008) Potential Use of Mangroves as Constructed Wetland for Municipal Sewage Treatment in Futian, Shenzhen, China. Marine Pollution Bulletin, 57, 735-743.

[12]   Pettit, N.M., Smith, A.R.J., Freedman, R.B. and Burns, R.G. (1976) Soil Urease: Activity, Stability and Kinetic Properties. Soil Biology and Biochemistry, 8, 479-484.

[13]   Klose, S. and Tabatabai, M.A. (1999) Urease Activity of Microbial Biomass in Soils. Soil Biology and Biochemistry, 31, 205-211.

[14]   Sinsabaugh, R.L. (1994) Enzymatic Analysis of Microbial Pattern and Process. Biology and Fertility of Soils, 17, 69-74.

[15]   McCarty, G.W., Shogren, D.R. and Bremner, J.M. (1992) Regulation of Urease Production in Soil by Microbial Assimilation of Nitrogen. Biology and Fertility of Soils, 12, 261-264.

[16]   Taylor, J.P., Wilson, B., Mills, M.S. and Burns, R.G. (2002) Comparison of Microbial Numbers and Enzymatic Activities in Surface Soils and Subsoils Using Various Techniques. Soil Biology and Biochemistry, 34, 387-401.

[17]   Borghetti, C., Gioacchini, P., Marzadori, C. and Gessa, C. (2003) Activity and Stability of Urease-Hydroxyapatite and Urease-Hydroxyapatitehumic Acid Complexes. Biology and Fertility of Soils, 38, 96-101.

[18]   Andrews, R.K., Blakeley, R.L. and Zerner, B. (1989) Urease: A Ni (II) Metalloenzyme. In: Lancaster, J.R., Ed., The Bioinorganic Chemistry of Nickel, VCH, New York, 141-166.

[19]   Byrnes, B.H. and Amberger, A. (1989) Fate of Broadcast Urea in a Flooded Soil When Treated with N-(nbutyl) Thiophospheric Triamide, a Urease Inhibitor. Fertility Research, 18, 221-231.

[20]   Simpson, J.R. and Freney, J.R. (1988) Interacting Processes in Gaseous Nitrogen Loss from Urea Applied to Flooded Rice Fields. In: Pushparajah, E., Husin, A., Bachik, A.T., Eds., Proceedings of International Symposium on Urea Technology and Utilization, Malaysian Society of Soil Science, Kuala Lumpur, 281-290.

[21]   Simpson, J.R., Freney, J.R., Wetselaar, R., Muirhead, W.A., Leuning, R. and Denmead, O.T. (1984) Transformations and Losses of Urea Nitrogen after Application to Flooded Rice. Australian Journal of Agricultural Research, 35, 189-200.

[22]   Rotini, O.T. (1935) La trasformazione enzimatica dell’urea nel terreno. Ann Labor Ric Ferm Spallanrani, 3, 143-154.

[23]   Polacco, J.C. (1977) Is Nickel a Universal Component of Plant Ureases? Plant Science Letters Journal, 10, 249-255.

[24]   Burns, R.G. (1986) Interaction of Enzymes with Soil Mineral and Organic Colloids. In: Huang, P.M. and Schnitzer, M., Eds., Interactions of Soil Minerals with Natural Organics and Microbes, Soil Science Society of America, Madison, 429-452.

[25]   Mobley, H.L.T. and Hausinger, R.P. (1989) Microbial Urease: Significance, Regulation and Molecular Characterization. Microbiological Reviews, 53, 85-108.

[26]   Zantua, M.I. and Bremner, J.M. (1977) Stability of Urease in Soils. Soil Biology and Biochemistry, 9, 135-140.

[27]   Tabatabai, M.A. (1977) Effect of Trace Elements on Urease Activity in Soils. Soil Biology and Biochemistry, 9, 9-13.

[28]   Yang, Z., Liu, S., Zheng, D. and Feng, S. (2006) Effects of Cadmium, Zinc and Lead on Soil Enzyme Activities. Journal of Environmental Sciences, 18, 1135-1141.

[29]   Trasar-Cepeda, C., Gil-Sotres, F. and Leiros, M.C. (2007) Thermodynamic Parameters of Enzymes in Grassland Soils from Galicia, NW Spain. Soil Biology and Biochemistry, 39, 311-319.

[30]   Grasshoff, E. and Kremling (1983) Standard Method for Sea Water Analysis. 2nd Edition.

[31]   Walkey, J.A. and Black, J.A. (1934) Estimation of Organic Carbon by the Chromic Acid Titration Method. Soil Science, 37, 29-31.

[32]   Richards, L.A. (1968) Diagnosis and Improvement of Saline and Alkali Soils. USDA Agriculture Hand Book No. 60. Oxford and IBH Publishing Co., New Delhi.

[33]   Subhajit, D., Minati, D., Raghab, R., Chumki, C., Tapan, K.J. and Tarun, K.D. (2011) Microbial Ecosystem in Sunderban Mangrove Forest Sediment, North-East Coast of Bay of Bengal, India. Geomicrobiology Journal, 29, 656-666.

[34]   Tiwari, S.C., Tiwari, B.K. and Mishra, R.R. (1989) Microbial Community, Enzyme Activity and CO2 Evolution in Pineapple Orchard Soil. Journal of Tropical Ecology, 30, 265-273.

[35]   Ellen, K. and Gerber, H. (1988) Short-Term Assay of Soil Urease Activity Using Colorimetric Determination of Ammonium. Biology and Fertility of Soils, 6, 68-72.

[36]   Paolini, J.E. and Sánchez-Arias, L.E. (2008) Comparative Biochemical Study of the Rhizosphere of Rhizophoramangle and Its Associated Species Cyperus sp. in the Ciénaga deSoledad (Colombia) In: Lieth, H., et al., Eds., Mangroves and Halophytes: Restoration and Utilisation, Springer Science + Business Media B.V., Berlin, 79-84.

[37]   Zhou, X., Zhang, Y. and Downing, A. (2011) Non-Linear Response of Microbial Activity across a Gradient of Nitrogen Addition to a Soil from the Gurbantunggut Desert, Northwestern China. Soil Biology and Biochemistry, 30, 1-11.

[38]   Pett-Ridge, J., Petersen, D.G., Nuccio, E. and Firestone, M.K. (2013) Influence of Oxic/Anoxic Fluctuations on Ammonia Oxidizers and Nitrification Potential in a Wet Tropical Soil. FEMS Microbiology Ecology, 85, 179-194.

[39]   Das, S., Sarkar, T.K., De, M., Ganguly, D., Maiti, T.K., Mukherjee, A., Jana, T.K. and De, T.K. (2011) Depth Profile Exploration of Enzyme Activity and Culturable Microbial Community from the Oxygen-Starved Soil of Sundarban Mangrove Forest, India. Open Journal of Ecology, 1, 65-72.

[40]   Das, S., Ganguly, D., Maiti, T.K., Mukherjee, A., Jana, T.K. and De, T.K. (2013) A Depth Wise Diversity of Free Living N2 Fixing and Nitrifying Bacteria and Its Seasonal Variation with Nitrogen Containing Nutrients in the Mangrove Sediments of Sundarban, WB, India. Open Journal of Marine Science, 3, 112-120.

[41]   Agehara, S. and Warncke, D.D. (2005) Soil Moisture and Temperature Effects on Nitrogen Release from Organic Nitrogen Sources. Soil Science Society of America Journal, 69, 1844-1855.