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 AJPS  Vol.8 No.7 , June 2017
In Vitro Bioassay of Allelopathy in Four Bamboo Species; Bambusa multiplex, Phyllostachys bambusoides, P. nigra, Sasa kurilensis, Using Sandwich Method and Protoplast Co-Culture Method with Digital Image Analysis
Abstract: Moderately strong allelopathic activities were found in four bamboo species, Bambusa multiplex cv. Houraichiku; Phyllostachys bambusoides cv. Madake; P. nigra cv. Hachiku; Sasa kurilensis cv. Chishimazasa, which are of different classification or of different ecological distributions, using the “Sandwich Method”, which assays the dried leaves on growth of lettuce seedlings. Only small difference of activity was found among the four bamboo species. In addition, Protoplast Co-culture Method” for assay of allelopathy in a 50 μL liquid medium using a 96 well culture plate, was applied to the suspension cultures of the four bamboo species. Protoplasts were isolated from two-week cultured suspension cells of four bamboo species using Cellulase RS and Pectolyase Y-23 in 0.6 M mannitol. At low protoplast densities of bamboo, B. multiplex and P. bambusoides stimulated the recipient lettuce growth, i.e., non-spherically cell enlargement and cell divisions observed under an inverted microscope, while protoplasts of P. nigra and S. kurilensis were less stimulatory or inhibitory. Inhibitory effect of S. kurilensis was the strongest among four bamboo species. Furthermore, highly inhibitory effects of S. kurilensis protoplasts on yellow color accumulation of lettuce protoplasts were clearly observed by analysis of a scanned digital image of a 96-well culture plate. Differences and causes of the allelopathic activities were discussed comparing with other plant species studied using the same assay methods.
Cite this paper: Ogita, S. , Sasamoto, H. , (2017) In Vitro Bioassay of Allelopathy in Four Bamboo Species; Bambusa multiplex, Phyllostachys bambusoides, P. nigra, Sasa kurilensis, Using Sandwich Method and Protoplast Co-Culture Method with Digital Image Analysis. American Journal of Plant Sciences, 8, 1699-1710. doi: 10.4236/ajps.2017.87117.
References

[1]   Fujii, Y. and Kobayashi, Y. (1994) Allelopathic Activities of Leaf Leachates of Bamboo and Sasa; Sandwich Method of 80 Species. Weed Biology and Management, 39, 94-95.

[2]   Fujii, Y. (2000) Allelopathy. Nobunkyo, Tokyo.

[3]   Fujii, Y., Pavez, S.S., Pavez, M.M., Ohmae, Y. and Iida, O. (2003) Screening of 239 Medicinal Plant Species for Allelopathic Activity using the Sandwich Method. Weed Biology and Management, 3, 233-241.
https://doi.org/10.1046/j.1444-6162.2003.00111.x

[4]   Fujii, Y., Shibuya, T., Nakatani, K., Itani, T., Hiradate, S. and Pavez, M.M. (2004) Assessment Method for Allelopathic Effect from Leaf Litter Leachates. Weed Biology and Management, 4, 19-23.
https://doi.org/10.1111/j.1445-6664.2003.00113.x

[5]   Sasamoto, H., Murashige-Baba, T., Inoue, A., Sato, T., Hayashi, S. and Hasegawa, A. (2013) Development of a New Method for Bioassay of Allelopathy Using Protoplasts of a Leguminous Plant Mucuna pruriens with a High Content of the Allelochemical L-DOPA. Journal of Plant Studies, 2, 71-80.

[6]   Hasegawa, A., oyanagi, T., Minagawa, R., Fujii, Y. and Sasamoto, H. (2014) An Inverse Relationship beween Allelopathic Activity and Salt Tolerance in Suspension Cultures of Three Mangrove Species, Sonneratia alba, S. caseolaris and S. ovata: Development of a Bioassay Method for Allelopathy, the Protoplast Co-Culture Method. Journal of Plant Research, 127, 755-761.
https://doi.org/10.1007/s10265-014-0651-1

[7]   Mori, D., Ogita, S., Fujise, K., Inoue, A. and Sasamoto, H. (2015) Protoplast Co-Culture Bioassay for Allelopathy in Leguminous Plants, Leucaena leucocephala and Mucuna gigantea, Containing Allelochemical Amino Acids, Mimosine and L-DOPA. Journal of Plant Studies, 4, 1-11.

[8]   Sasamoto, H. and Ashihara, H. (2014) Effect of Nicotinic Acid, Nicotinamide and Trigonelline on the Proliferation of Lettuce Cells Derived from Protoplasts. Phytochemistry Letters, 7, 38-41.
https://doi.org/10.1016/j.phytol.2013.09.008

[9]   Inoue, A., Mori, D., Minagawa, R., Fujii, Y. and Sasamoto, H. (2015) Allelopathy in a Leguminous Mangrove Plant, Derris indica: Protoplast Co-Culture Bioassay and Rotenone Effect. Natural Product Communications, 5, 747-750.

[10]   Sasamoto, H., Fujii, Y. and Ashihara, H. (2015) Effect of Purine Alkaloids on the Proliferation of Lettuce Cells Derived from Protoplasts. Natural Product Communications, 5, 751-754.

[11]   Sasamoto, H., Murashige-Baba, T., Sato, T., Hasegawa, A., Wasano, N. and Fujii, Y. (2015) Development of a Simple High-Throughput Bioassay Method for Allelopathy: Protoplast Co-Culture with Image Analysis. Abstracts of the 2015 in Vitro Biology Meeting. In Vitro Cellular & Developmental Biology-Plant, 51, 12.

[12]   Sasamoto, H., Azumi, Y. and Suzuki, S. (2017) Development of a High-Throughput Bioassay Method of Allelopathy-Protoplast Co-Culture and Digital Image Analysis. Science Journal of Kanagawa University, 28, 65-72.

[13]   Ogita, S. (2005) Callus and Cell Suspension Culture of Bamboo Plant, Phyllostachys nigra. Plant Biotechnology, 22, 119-125.
https://doi.org/10.5511/plantbiotechnology.22.119

[14]   Ogita, S., Ohki, S., Nomura, T. and Kato, Y. (2012) A β-Glucosidase Activity Potentially Involved in Cell Division and Wall Development of Phyllostachys Bamboo Suspension Cells. American Journal of Plant Sciences, 3, 1066-1072.
https://doi.org/10.4236/ajps.2012.38127

[15]   Ogita, S., Kishimoto, T., Nomura, T. and Kato, T. (2016) Chapter 7: Plant Cell, Tissue, and Organ Culture Approaches to Explore the Functional Cell Differentiation in Phyllostachys and Bambusa Bamboo Plants. In: Ramawat, K.G. and Ahuja, M.R., Eds., Fiber Plants: Biology, Biotechnology and Applications, Springer, Berlin, 111-126.
https://doi.org/10.1007/978-3-319-44570-0_7

[16]   Ogita, S., Ohki, S. and Kato, Y. (2008) Uptake of Carbohydrate by Suspension Cultured Cells of Bamboo Plants. In: FOPB Phyllostachys 1-5, Global Science Books 24.

[17]   Ogita, S., Kikuchi, N., Nomura, T. and Kato, Y. (2011) A Practical Protocol for Particle Bombardment-mediated Transformation of Phyllostachys Bamboo Suspension Cells. Plant Biotechnology, 28, 43-50.
https://doi.org/10.5511/plantbiotechnology.10.1101a

[18]   Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Growth and Bioassay with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497.
https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

[19]   Larkin, P.J. (1976) Purification and Viability Determination of Plant Protoplasts. Planta, 128, 213-216.
https://doi.org/10.1007/BF00393231

[20]   Rasband, W.S. (1997-2016). Image J.U.S. National Institute of Health, Bethesda, Maryland, USA.
http://imagej.nih.gov/ij

[21]   Chou, A.-H. and Yang, C.-M. (1982) Allelopathic Research of Subtropical Vegetation in Taiwan II. Comparative Exclusion of Understory by Phyllostachys edulis and Cryptomeria japonica. Journal of Chemical Ecology, 8, 1489-1507.
https://doi.org/10.1007/BF00989105

[22]   Mu, J., Uehara, T. and Furuno, T. (2006) Regulation Effect of Phyllostachys pubescens Methanol Extractives on Growth of Seed Plants. Journal of Wood Science, 52, 367-371.
https://doi.org/10.1007/s10086-005-0768-x

[23]   Ogita, S. (2015) Plant Cell, Tissue and Organ Culture: the Most Flexible Foundations for Plant Metabolic Engineering Applications. Natural Product Communications, 10, 815-820.

 
 
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