Back
 AS  Vol.8 No.5 , May 2017
A Study on the Aseptic Germination Method for Rosa rugose Seeds
Abstract: In order to find a method to break dormancy of Rosa rugose seeds and interspecific hybrid seeds between Rosa rugose and Rosa hybrid quickly, and accelerate the breeding process of interspecific hybridization between Rosa rugose and Rosa hybrid, the influence of concentrated acid, seed maturity, GA3 (gibberellin) and low temperature (4°C) on seed germination of Rosa rugose from Muping was researched under aseptic condition. The results showed that aseptic germination can significantly shorten the germination time of Rosa rugose seeds and raise its germination ratio. Before inoculation, concentrated acid treatment greatly increased the germination rate and reduced the contamination rate of the seeds. The higher the degree of maturity of seeds is, the lower the germination rate would be, and the best time for seed to aseptic germination is 60 d after pollination. The addition of GA3 in 1/2MS medium could promote seeds germination better, and when the concentration of GA3 was 0.15 mg/L, the seed germination ratio was the highest; the germination time decreased and the seed germination ratio increased gradually as the treatment time at 4°C lasted longer.
Cite this paper: Fu, C. , Xing, S. , Zhao, D. , Zang, D. , Wei, Y. , Zhao, L. and Yu, X. (2017) A Study on the Aseptic Germination Method for Rosa rugose Seeds. Agricultural Sciences, 8, 426-434. doi: 10.4236/as.2017.85032.
References

[1]   Feng, L.G., Sheng, L.X., Zhao, L.Y., et al. (2008) Changes of Aromatic Components and Contents in the Process of Rosa rugosa Development. Chinese Agricultural Science, 41, 4341-4351.

[2]   Wen, J.L. and Xue, Z.D. (2000) Cold Resistance and Plasma Membrane Permeability of Rosa rugosa. Journal of Northwest Forestry College, 15, 16-20.

[3]   Liu, J. (2013) Study on the Cross Breeding and Hybrid Compatibility between Rosa rugosa and Modern Rosa hybrid. Beijing Forestry University, Beijing.

[4]   Wang, Q. (2010) Preliminary Study on Cross of Rosa cvs. and Rosa rugosa and Resistance to Black Spot of Rose. Beijing Forestry University, Beijing.

[5]   Zhang, Y., Liu, T., Zhang, H.X., et al. (2010) Advances in Breeding and Tissue Culture of Rosa hybrid. Chinese Agricultural Bulletin, 26, 250-254.

[6]   Yu, X.Y. (2010) Study on Embryology and Pollination Compatibility of Rosa rugosa. Shandong Agricultural University, Taian.

[7]   Gibson, M. (1984) Growing Roses. Groomhelm Ltd., 201-204.

[8]   Ackson, G.A.O. and Blundell, J.B. (1963) Germination in Rosa. Journal of Horticultural Science, 38, 310-320.
https://doi.org/10.1080/00221589.1963.11514082

[9]   Zhou, Z.X. and Bao, W.K. (2009) Dormancy and Methods to Relieve of Rose Seed. Journal of Tropical and Subtropical Botany, 6, 621-628.

[10]   Li, Z.W., Zhong, L. and Zhao, L.J. (2011) Study on Dormancy and Germination Uniformity of Wild Rosa rugosa Seed. Seed, 10, 42-44.

[11]   Yu, X.Y., Xing, S.T. and Zhao, L.Y. (2014) Analysis of the Causes of Interspecific Hybridization Barrier Between Rosa rugosa and Rosa hybrid. China Agriculture Science, 15, 3112-3120.

[12]   Xue, T.T., Shen, Y.B., Liu, J., et al. (2016) Research Progress on Seed Dormancy. Acta Botanica Sinica, 6, 863-871.

[13]   Zhang, N.N. (2009) Study on Seed Characteristics of Rosa rugosa (Rosa rugosa Thunb.). Shandong Agricultural University, Shang Dong.

[14]   Gama-Arachchige, NS., Baskin, J.M., Geneve, R.L. and Baskin, C.C. (2011) Acquisition of Physical Dormancy and Ontogeny of the Micropyle-Water-Gap Complex in Developing Seeds of Geranium carolinianum (Geraniaceae). Annals of Botany, 108, 51-64.
https://doi.org/10.1093/aob/mcr103

[15]   Yang, Q.H. (2003) The Cause of Dormancy and Dormancy Polymorphism of Plant Seeds. Acta Botanica Boreali-Occidentalia Sinica, 5, 837-845.

[16]   Baskin, C. and Baskin, J.M. (2014) Seeds: Ecology, Biogeography, and Evolution of Dormancy and Germination. Academic Press, San Diego, 150-162.

[17]   Han, Y. and Zhao, L. (2006) Study on Seed Rapid Germination of Rosa multiflora in Sterile Conditions. Chinese Agricultural Science Bulletin, 6, 285-288.

[18]   Guo, M.Y., Quan, B.W. and Liu, H.F. (2006) Study on the Seed Germination of Rosa multiflora. Gansu Agriculture, 8, 157-158.

[19]   Zhou, Z.Q. and Bao, W.K. (2011) Levels of Physiological Dormancy and Methods for Improving Seed Germination of Four Rosa multiflora Species. Scientia Horticulturae, 129, 818-824.

[20]   Bi, J.C. (2015) The Dormancy Mechanism and Removing Method Hard Seed. Agricultural Development and Equipment, 3, 83.

[21]   Yu, M., Xu, H., Zhang, H., et al. (2016) The Regulation Mechanism of Plant Hormones on Seed Dormancy and Germination. Journal of Plant Physiology, 5, 599-606.

 
 
Top