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 OJST  Vol.1 No.3 , September 2011
Qualitative analysis of re mineralized carious lesions subjected to fluoride supplement through confocal laser scanning microscope
Abstract: Aim: 1] Comparative evaluation of the linear depth of induced remineralized lesions after subjecting to fluoride supplements and 2] To assess the average fluorescence at both the demineralized and the remi-neralized zones in all the three study groups under confocal laser scanning microscope. Method: Forty five sound human premolars extracted for orthodon-tic reasons were decoronated 1 mm below the ce-mento-enamel junction and coated with nail varnish except for a 3 × 3 mm window on the buccal surface. The samples were placed in 50 ml of de mineralizing solution at pH 4.6 for 96 hours. Following deminera-lization, the lower half of the 3 × 3 mm window in all the samples were covered with nail varnish to serve as control. The samples were randomly divided into three groups of fifteen teeth each (n = 15) and speci-mens in group A[Nfd] were remineralized using non-fluoridated dentifrice [control], those in groups B [Fd5] and group C [Fd10] using 500 ppm and 1000 ppm of fluoride containing dentifrice, respectively. The specimens were subjected to a 20 day reminera-lization treatment regimen and were sectioned into 100 µm thick sections and two images were captured on the buccal surface from either side of the midpoint of occluso-cervical length using confocal laser scan-ning microscope [CLSM]. Results: were tabulated and statistically analyzed by Anova. Study concluded that 1000 ppm fluoridated dentifrice showed a greater degree of remineralization than other groups and confocal laser scanning microscopes gives promising results in the diagnosis of early enamel lesions over the conventional methods.
Cite this paper: nullShashikala, K. and Sheela, N. (2011) Qualitative analysis of re mineralized carious lesions subjected to fluoride supplement through confocal laser scanning microscope. Open Journal of Stomatology, 1, 55-60. doi: 10.4236/ojst.2011.13010.
References

[1]   Sturdevant, C.M., Roberson, T.M. and Heymann, H.O. (2006) The art and science of operative dentistry. 5th Edition, Mosby Publishers.

[2]   Queiroz, C.S. and Hara, A.T. (2008) PH-cycling models to evaluate the effect of low fluoride dentifrice on enamel de- and remineralization. Brazilian Dental Journal, 19, 21-27. doi:10.1590/S0103-64402008000100004

[3]   Kishen, A., Shrestha, A. and Rafique, A. (2008) Fiber optic backscatter spectroscopic sensor to monitor enamel demineralization and remineralization in vitro. Journal of Conservative Dentistry, 11, 63-70. doi:10.4103/0972-0707.44053

[4]   Ferriera-Zandona, A.G., Analoui, M. and Beiswanger, B.B. (1998) An in vitro comparison between laser fluorescence and visual examination for detection of demineralization in occlusal pits and fissures. Caries Research, 32, 210-218. doi:10.1159/000016455

[5]   Hicks, J., Garcia-Godoy, F. and Flaitz, C. (2004) Biolog-ical factors in dental caries: role of saliva and dental plaque in the dynamic process of demineralization and re-mineralisation (Part II). Journal of Clinical Pediatric Dentistry, 28, 119-124.

[6]   Kumar, V.L.N., Itthagarun, A. and King, N.M. (2008) The effect of casein phosphopeptide-amorphous calcium phosphate on the remineralization of artificial caries-like lesions: An in vitro study. Australian Dental Journal, 53, 34-40. doi:10.1111/j.1834-7819.2007.00006.x

[7]   Zaura, E., ten Cate, J.M. (2004) Dental plaque as a biofilm: A pilot study of the effects of nutrients on plaque pH and dentin demineralization. Caries Research, 38, 9-15. doi:10.1159/000074357

[8]   Fontana, M., Li, Y. and Dunipace, A.J. (1996) Measure-ment of enamel demineralization using microradiography and confocal microscopy. A correlation study. Caries Research, 30, 317-325. doi:10.1159/000262337

[9]   Watson, T.F. (1991) Applications of confocal scanning optical microscopy to dentistry. British Dental Journal, 171, 287-291. doi:10.1038/sj.bdj.4807695

[10]   Gonzalez-Cabezas, C. and Fontana, M. (1998) Mea-surement of enamel remineralization using microradio-graphy and confocal microscopy. Caries Research, 32, 385-392. doi:10.1159/000016475

[11]   Hall, A.F., DeSchepper, E. and Ando, M. (1997) In vitro studies of laser fluorescence for detection and quantifica-tion of mineral loss from dental caries. Advance Dental Research, 11, 507-514. doi:10.1177/08959374970110041901

[12]   Hafstrom-Bjorkman, U. (1996) Comparison of laser fluo-rescence and longitudinal microradiography for quantita-tive assessment of in vitro enamel caries. Caries Research, 26, 241-247. doi:10.1159/000261446

[13]   Wefel, J.S. and Harless, J.D. (1984) Comparison of ar-tificial white spots by microradiography and polarized light microscopy. Journal of Dental Research, 63, 1271-1275. doi:10.1177/00220345840630110301

 
 
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