AiM  Vol.7 No.5 , May 2017
Microorganisms Associated with Vegetable Oil Polluted Soil
Abstract: Vegetable oil Spills are becoming frequent and are potentially more challenging than petroleum hydrocarbon spills. Microbial lipases occupy a place of prominence among biocatalysts are often used for remediation of vegetable oil-polluted sites. This work was carried out to isolate microorganisms from oil-polluted sites and screen them for their lipolytic activity. Microorganisms were isolated from eight experimental soil samples contaminated with different types of vegetable oil, soil from an oil mill in Ibadan, and normal uncontaminated soil as a control. The isolates were characterized, identified and those common to at least one of the experimental sites and oil mill sites were screened for their lipolytic activity. Data obtained were analysed using Duncan Multiple Range Test. Seventy three microorganisms were isolated from the polluted soil and identified as species of Bacillus (16), Pseudomonas (12), Flavobacterium (6), Alcaligenes (2), Proteus (3), Micrococcus (1), Aspergillus (9), Penicillium (6), Saccharomyces (4), Geotrichum (1), Kluveromyces (1). Bacillus subtilis, Bacillus licheniformic, Pseudomonas cepacia, Pseudomonas fluorescens, Flavobacterium sp., Alcaligenes sp. and Candida parapsilosis which were common to at least one of the experimental site and oil mill site were preliminarily screened for lipolytic activity and all nine confirmed by presence of halos around the colonies. These screened organisms have potential for the degradation of fatty waste. They could therefore be employed in environmental clean-up of vegetable oil spill site.
Cite this paper: Popoola, B. and Onilude, A. (2017) Microorganisms Associated with Vegetable Oil Polluted Soil. Advances in Microbiology, 7, 377-386. doi: 10.4236/aim.2017.75031.


[2]   Singh, A.K. and Mukhopadhyay, M. (2012) Overview of Fungal Lipase: A Review. Applied Biochemistry and Biotechnology, 166, 186-520.

[3]   Nagarajan, S. (2012) New Tools for Exploring “Old Friends—Microbial Lipases”. Applied Biochemistry and Biotechnology, 168, 1163-1196.

[4]   Abrunhosa, L., Oliveira, F., Dantas, D., Goncalves, C. and Belo, I. (2013) Lipase Production by Aspergillus ibericus Using Olive Mill Waste Water. Bioprocess and Biosystem Engineering, 36, 285-291.

[5]   Choo, D., Kurihara, T., Suzuku, T., Soda, K. and Esaki, N. (1998) A Cold-Adapted Lipase of an Alaskan Psychrotroph, Pseudomonas sp. Strain B11-1: Gene Cloning and Enzyme Purification and Characterization. Applied and Environmental Microbiology, 64, 486-491.

[6]   Sztajer, H., Maliszewska, I. and Weiczorek, J. (1988) Production of Exogenous Lipases by Bacteria, Fungi, and Actinomycetes. Enzyme and Microbial Technology, 10, 492-497.

[7]   Kulkarni, N. and Gadre, R.V. (2002) Production and Properties of an Alkaline, Thermophilic Lipase from Pseudomonas fluorescens NS2W. Journal of Industrial Microbiology and Biotechnology, 28, 344-348.


[9]   Harrigan, W.F. and McCance, M.E. (1976) Laboratory Methods in Food and Dairy Microbiology. Academic Press Inc. Limited, London.

[10]   Sneath, P.H.A. (1986) Bergey’s Manual of Systematic Bacteriology. Vol. 2, The Williams and Wilkins Co., Baltimore.

[11]   Gogoi, B.K., Dutta, N.N., Goswani, P. and Krishna, M.T.R. (2003) A Case Study of Bioremediation of Petroleum-Hydrocarbon Contaminated Soil at Crude Oil Spill Site. Advances in Environmental Research, 7, 767-782.

[12]   Haliru, M. and Bukola, C.A. (2012) Screening of Microorganisms Isolated from Different Environmental Samples for Extracellular Lipase Production. AU J.T., 15, 179-186.

[13]   Gupta, R., Gupta, N.J. and Rathi, P. (2004) Bacterial Lipase: An Overview of Production, Purification and Biochemical Properties. Applied Microbiology and Biotechnology, 64, 763-781.

[14]   D’Annibale, A., Sermanni, G.G. and Federici, F. (2006) Olive-Mill Wastewaters a Promising Substrate for Microbial Lipase Production. Bioresource Technology, 97, 1823-1833.

[15]   Riaz, M., Shah, A.A., Hammed, A. and Hasan, F. (2010) Characterization of Lipase Produced by Bacillus sp. FHS in Immobilized and Free State. Annals of Microbiology, 60, 169-175.

[16]   Nwuche, C.O. and Ogbonna, J.C. (2011) Isolation of Lipase Producing Fungi from Palm Oil Mill Effluent (POME) Dump Sites at Nsukka. Brazilian Archives of Biology and Technology, 54, 113-116.

[17]   Thota, P., Bhogavalli, P.K., Vallem, P.R. and Screerangam, V. (2012) Biochemical Characterization of an Extracellular Lipase from New Strain of Rhizopus sp. Isolated from Oil Contaminated Soil. International Journal of Plant Animal and Environmental Sciences, 2, 41-45.

[18]   Gopinath, S.C.B., Anubu, P., Lakshmipnya, T. and Hilda, A. (2013) Strategies to Characterize Fungal Lipases for Applications in Medicine and Dairy Industry. BioMed Research International, 2013, Article ID: 154549.

[19]   Kanchana, R., Muraleedharan, U.D. and Raghukumar, S. (2011) AAlkaline Lipase Activity from the Marine Protists, Thraustochytrids. World Journal of Microbiology and Biotechnology, 27, 2125-2131.

[20]   Sharma Chander, K. and Kanwar Shamsher, S. (2001) Purification of a Novel Thermophilic Lipase from Bacillus licheniformis MTCC-10498. ISCA Journal of Biological Sciences, 1, 43-48.

[21]   Saxena, R.K., Sheoran, A., Giri, B. and Davidson, W.S. (2002) Purification Strategies for Microbial Lipases. Journal of Microbiological Methods, 52, 1-18.

[22]   Chakraborty, K. and Raj, K.R. (2008) An Extra-Cellular Alkaline Metallolipase from Bacillus licheniformis MTCC 6824: Purification and Biochemical Characterization. Food Chemistry, 109, 727-736.

[23]   Sharma, R.K., Jethani, B., Raipuria, M. and Jain, H. (2011) Antipyretic Activity of Aqueous and Alcoholic Extracts of Nonion Yeast Induced Pyrexia in Rats. International Journal of Pharmaceutical Sciences and Research, 2, 1850-1854.

[24]   Rohit, S., Yusuf, C. and Uttam, C.B. (2001) Production, Purification, Characterization and Applications of Lipases. Biotechnology Advances, 19, 627-662.

[25]   Shabtai, Y. (1991) Isolation and Characterization of a Lipolytic Bacterium Capable of Growing in a Low-Water Content Oil Water-Emulsion. Applied and Environmental Microbiology, 57, 1740-1745.

[26]   Shabtai, Y.A. and Daya-Mishire, N. (1992) Production, Purification and Properties of Lipase from a Bacterium Pseudomonas aeruginosa Y57. Capaolie of Growing in Water Restricted Environment. Applied and Environmental Microbiology, 58, 174-180.

[27]   Sigurgisledottir, S., Sonraodottir, M., Jonsson, A., Kristjarsson, J.K. and Mattheasson, E. (1993) Lipase Activity of Thermophilic Bacteria from Icelandic Hot Springs. Biotechnology Letters, 15, 361-366.

[28]   Loo, J.L., Lai, O.M., Long, K. and Ghazali, H.M. (2006) Identification and Characterization of a Locally Isolated Lipolytic Microfungus Geotrichum candidium. Malays Journal of Microbiology, 2, 22-29.