AJPS  Vol.2 No.3 , September 2011
Analysis of Genetic Diversity in Poeciloneuron pauciflorum Bedd.―An Endemic Tree Species from the Western Ghats of India
Abstract: Poeciloneuron pauciflorum is a narrow endemic having highly restricted distribution in the southern-western Ghats region of India. 18 accessions of P. pauciflorum collected from four different locations were analyzed for genetic variation using 20 random primers. Out of 141 amplicons generated, 130 of them were polymorphic (92.20% polymorphism). Contrary to the general concept of low genetic variation associated with endemic plant species, P. pauciflorum exhibits high genetic diversity as the similarity index value based on Nei & Li’s similarity coefficient ranges from 0.36 to 0.95 with mean GS = 0.72 and Shannon’s information measure (0.43). Cluster analysis showed grouping of all accessions from the State of Tamil Nadu into two major clusters with few outliers while those from the State of Kerala also clustered with them. Accessions from Kalliankadu forest segment harbors maximum diversity as indicated by various genetic diversity indices like h, I, Ht, Hs, Gst, PL and hence this site is recommended for in situ conservation of this narrow endemic. The main factors responsible for the high level of genetic diversity among accessions are probably related to the reproductive isolation and ecological breadth. The strong genetic variability among accessions indicates that the management for the conservation of the genetic diversity in P. pauciflorum should aim to preserve every accession. The present study assumes significance as it negotiates endemism and genetic variation in tree species, a global phenomenon having wide implications in species diversity and conservation.
Cite this paper: nullPillai, P. , Sajan, J. , Menon, K. , Hemanthakumar, A. , Pandurangan, A. , Krishnan, P. and Seeni, S. (2011) Analysis of Genetic Diversity in Poeciloneuron pauciflorum Bedd.―An Endemic Tree Species from the Western Ghats of India. American Journal of Plant Sciences, 2, 416-424. doi: 10.4236/ajps.2011.23047.

[1]   R. Gopalan and A. N. Henry, “Endemic Plants of India- Endemics of Agasthiyamalai Hills,” Bishen Singh Ma- hendrapal Singh publications, Dehradun, 2000.

[2]   D. Narasimhan and Sheeba J. Irwin, “Population Status of Poeciloneuron pauciflorum Bedd. (Clusiaceae): An En- demic and Critically Endangered Tree Species from Southern Western Ghats, India,” Indian Journal of Forestry, Vol. 33, No. 3, 2010, pp. 419-424.

[3]   N. Mohanan, T. Shaju, M. S. Raj Kiran and N. Ravi, “Rediscovery of Poeciloneuron pauciflorum Bedd. (Bonnetiaceae): An Endemic and Little Known Species of Western Ghats, Presumed to Be Extinct,” Annals of Forestry, Vol. 7, 1999, pp. 87-89.

[4]   R. H. Beddome, “On a New Genus of Ternstroemiaceae: Poeciloneuron from Nilgiris,” Journal of Linnean society, Vol. 8, 1865, p. 267.

[5]   S. Rajkumar and M. K. Janarthanam, “Agasthiyamalaia (Clusiaceae): A New Genus for Poeciloneuron pauciflorum, an Endemic and Endangered Tree of Western Ghats, India,” Journal of the Botanical Research Institute of Texas, Vol. 1, No. 1, 2007, pp. 129-133.

[6]   M. U. S. Sultanbawa, “Xanthonoids of Tropical Plants,” Tetrahedron, Vol. 36, 1980, pp. 1465-1506.

[7]   G. J. Bennett and H. K. Lee, “Xanthones from Guttiferae,” Phytochemistry, Vol. 28, 1989, pp. 967-998.

[8]   T. Hideki, I. Munekazu, I. M. Koh, I. Tetsuro, T. Toshiyuki, C. Veliah and R. Soedarsono, “Three Xanthones from Poeciloneruon pauciflorum and Mammea acuminate,” Phytochemistry, Vol. 45, No. 1, 1997, pp. 133-136.

[9]   M. V. Ignatushchenko, R. W. Winter and M. Riscoe, “Xanthones as Antimalarial Agents, Stage Specificity,” American Journal of Tropical Medicine and Hygiene, Vol. 62, 2000, pp. 77-81.

[10]   V. Rukachaisirikul, M. Kamkaew, D. Sukavisit, S. Phon- gpaichit, P. Sawangchote and W. Taylor, “Antibacterial Xanthones from the Leaves of Garcinia nigrolineata,” Journal of Natural Products, Vol. 66, No. 12, 2003, pp. 1531-1535. doi:10.1021/np0303254

[11]   V. Rukachaisirikul, K. Tadpetch, A. Watthanaphanit, N. Saengsanae and S. Phongpaichit, “Benzopyran, Biphenyl, and Tetraoxygenated Xanthone Derivatives from the Twigs of Garcinia nigrolineata,” Journal of Natural Products, Vol. 68, No. 8, 2005, pp. 1218-1221. doi:10.1021/np058050a

[12]   Y. Sukpondma, V. Rukachaisirikul and S. Phongpaichit, “Xanthone and Sesquiterpene Derivatives from the Fruits of Garcinia scortechinii,” Journal of Natural Products, Vol. 68, No. 7, 2005, pp. 1010-1017. doi:10.1021/np058050a

[13]   C. Ito, M. Itoigawa, T. Takakura, N. Ruangrungsi, F. Enjo, H. Tokuda, H. Nishino and H. Furukawa, “Chemical Constituents of Garcinia fusca: Structure Elucidation of Eight New Xanthone and Their Cancer Chemopreventive Activity,” Journal of Natural Products, Vol. 66, No. 2, 2003, pp. 200-205. doi:10.1021/np020290s

[14]   J. Zou, D. Jin, W. Chen, J. Wang, Q. Liu, X. Zhu and W. Zhao, “Selective Cyclooxygenase-2 Inhibitors from Calophyllum membranaceum,” Journal of Natural Products, Vol. 68, No. 10, 2005, pp. 1514-1518. doi:10.1021/np0502342

[15]   H. Oku, Y. Ueda, M. Iinuma and K. Ishiguro, “Inhibitory Effects of Xanthones from Guttiferae Plants on PAF-Induced Hypotension in Mice,” Planta Medica, Vol. 71, No. 1, 2005, pp. 90-92. doi:10.1055/s-2005-837760

[16]   K. S. Ghanthi and V. S. Manickam, “Ethnobotanical Utilization of Poeciloneuron pauciflorum Bedd. by the Kani Tribes of Agasthiamalai, Western Ghats, Tamil Nadu, India,” Ethnobotanical Leaflets, Vol. 12, 2008, pp. 719-722.

[17]   S. Nayak, G. R. Rout and P. Das, “Evaluation of the Genetic Variability in Bamboo Using RAPD Markers,” Plant, Soil and Environment, Vol. 49, No. 1, 2003, pp. 24-28.

[18]   M. Das, S. Bhattacharya, J. Basak and A. Pal, “Phylogenetic Relationships among the Bamboo Species as Revealed by Morphological Characters and Polymorphism Analysis,” Biologica Plantarum, Vol. 51, No. 4, 2007, pp. 667-672. doi:10.1007/s10535-007-0140-7

[19]   S. M. S. D. Ramanayake, V. N. Meemaduma and T. E. Weerawardene, “Genetic Diversity and Relationships between Nine Species of Bamboo in Sri Lanka, Using Random Amplified Polymorphic DNA,” Plant Systematics and Evolution, Vol. 269, No. 1-2, 2007, pp. 55-61. doi:10.1007/s00606-007-0587-1

[20]   S. Narasimhan, P. Padmesh and G. M. Nair, “Assessment of Genetic Diversity in Coscinium fenestratum,” Biologia Plantarum, Vol. 50, No. 1, 2006, pp. 111-113. doi:10.1007/s10535-005-0082-x

[21]   P. Padmesh, J. V. Reji, D. M. Jinish and S. Seeni, “Estimation of Genetic Diversity in Varities of Mucuna pruriens using RAPD,” Biologica Plantarum, Vol. 50, No. 3, 2006, pp. 367-372. doi:10.1007/s10535-006-0051-z

[22]   H. Q. Zhang and Y. H. Zghou, “Genetic Relationships among Hystrix patula, H.duthiei and H. longearistata According to Meiotic Studies and Genome-Specific RAPD Assay,” Biologica Plantarum, Vol. 53, No. 1, 2009, pp. 45-52. doi:10.1007/s10535-009-0007-1

[23]   P. Padmesh, J. V. Reji, P. C. Benadict, S. Mukunthakumar, G. Praveen and S. Seeni, “Analysis of Genetic Variability in Two Diploid Musa Cultivars Using RAPD,” Biologica Plantarum, Vol. 53, No. 4, 2009, pp. 711-714. doi:10.1007/s10535-009-0128-6

[24]   M. G. Murray and W. F. Thompson, “Rapid Isolation of High-Molecular Weight Plant DNA,” Nucleic Acids Research, Vol. 8, No. 19, 1980, pp. 4321-4325. doi:10.1093/nar/8.19.4321

[25]   E. V. Wulff, “An Introduction to Historical Plant Geography,” Chronica Botanica, Waltham, 1943.

[26]   I. V. Yap, R. J. Nelson, “In WinBoot: A programme for Performing Bootstrap Analysis for Binary Data to Determine the Confidence Limits of UPGMA Based Dendrograms,” International Rice Research Institute, Manila, 1996.

[27]   F. C. Yeh, T. B. J. Boyle, Z. H. Ye and J. X. Mao, “PO- PGENE: The User-Friendly Software for Population Ge- netic Analysis,” University of Alberta, Edmonton, 1999.

[28]   M. Nei, “Molecular Evolution Genetics,” Columbia University press, New York, 1987.

[29]   S. Wright, “The Genetic Structure of Populations,” Annals of Eugenetics, Vol. 16, 1931, pp. 97-159.

[30]   C. W. Michael and E. M. David, “Indirect Measures of Gene Flow and Migration: FST≠1/(4Nm+1),” Heredity, Vol. 82, 1999, pp. 117-125.

[31]   J. Antonovics and S. Via, “Genetic Influences on the Distribution and Abundance of Plants,” In: A. J. Devy, M. J. Hutchings and A. R. Watkinson, eds., Perspectives on Plant Population Biology, Sinauer Associates, Sunderland, 1987, pp. 185-203.

[32]   M. D. Loveless and J. L. Hamrick, “Ecological Determinants of Genetic Structure in Plant Populations,” Annual Review of Ecology and Evolutions, Vol. 15, 1984, pp. 65-95.

[33]   S. Wright, “The Genetic Structure of Populations,” Annals of Eugenetics, Vol. 16, 1931, pp. 97-159.

[34]   B. A. Schaal, D. A. Hayworth, K. M. Olsen, J. T. Rausher and W. A. Smith, “Phylogeographical Studies in Plant: Problems and Prospects,” Molecular Ecology, Vol. 7, No. 4, 1998, pp. 465-474. doi:10.1046/j.1365-294x.1998.00318.x

[35]   M. C. Whitlock and D. E. McCauley, “Some Population Genetic Consequences of Colony Formation and Extinction: Genetic Correlations within Founding Groups,” Evolution, Vol. 44, 1990, pp. 1717-1724.

[36]   J. L. Hamrick and M. J. W. Godt, “Conservation Genetics of Endemic Plant Species,” In: J. C. Avise and J. L. Hamrick, eds., Conservation Genetics, Case Histories from Nature, Chapman & Hall, New York, 1996a, pp. 281-304.

[37]   J. L. Hamrick and M. J. W. Godt, “Effects of the History Traits on Genetic Diversity in Plants,” Philosophical Transactions of the Royal Society of London Biological Sciences, Vol. 351, 1996b, pp. 129-129.

[38]   Savolainen and H. Kuittien, “Small Populations Processes,” In: A. Young, D. Boshier and T. Boyle, eds., Forest conservation genetics, CABI Publishing, Collingwood, 2000, pp. 91-100.

[39]   G. L. Stebbins, “Rarity of Plant Species―A Synthetic View Point,” Rhodora, Vol. 82, No. 829, 1980, pp. 77-86.

[40]   M. A. Gitzendanner and P. A. Solitis, “Patterns of Genetic Variation in Rare and Widespread Plant Congeners,” American Journal of Botany, Vol. 87, No. 6, 2000, pp. 783-792. doi:10.2307/2656886

[41]   S. C. H. Barrett, J. K. Kohn, “Genetic and Evolutionary Consequences of Small Population Size Inplants: Implications for Conservation,” In: D. A. Falk and K. E. Holsinger, eds., Genetics and Conservation of Rare Plants, Oxford University Press, Oxford, 1991, pp. 3-30.

[42]   N. C. Ellstrand and D. R. Elam, “Population Genetic Consequences of Small Population Size: Implications for Plant Conservation,” Annual Review of Ecology and Systematics, Vol. 24, 1993, pp. 217-242. doi:10.1146/

[43]   A. H. D. Brown, “Enzyme Polymorphism in Plant Populations,” Theoretical Population Biology, Vol. 15, No. 1, 1979, pp. 1-42. doi:10.1016/0040-5809(79)90025-X

[44]   T. S. Richter, P. S. Soltis, D. E. Soltis, “Genetic Variation within and Among Populations of the Narrow Endemic, Delpbinuim viridescens (Ranunculaceae),” American Journal of Botany, Vol. 81, No. 8, 1994, pp. 1070-1076. doi:10.2307/2445302

[45]   A. U. Deshpande, G. S. Apte, et al., “Genetic Diversity across Natural Populations of Three Montane Plant Species from the Western Ghats, India Revealed by Intersimple Sequence Repeats,” Molecular Ecology, Vol. 10, No. 10, 2001, pp. 2361-2367. doi:10.1046/j.0962-1083.2001.01379.x

[46]   Da-Wei Xue, Xue-Jun Ge, Gang Hao and Chang-Qin Zhang, “High Genetic Diversity in a Rare, Narrowly Endemic Primrose Species: Primula interjacens by ISSR Analysis,” Acta Botanica Sinica, Vol. 46, No. 10, 2004, pp. 1163-1169.

[47]   U. Kang, C. S. Chang and Y. S. Kim, “Genetic Structure and Conservation Considerations of Rare Endemic Abeliophyllum Distichum Nakai (Oleaceae) in Korea,” Journal of Plant Resources,Vol. 113, 2000, pp. 127-138.

[48]   J. Gonzalez-Astorga and G. Castillo-Campos, “Genetic Variability of the Narrow Endemic Tree Antirhea aromatica Castillo-Campos and Lorence (Rubiaceae, Guettardeae) in a Tropical Forest of Mexico,” Annals of Botany, Vol. 93, No. 5, 2004, pp. 521-528. doi:10.1093/aob/mch070

[49]   R. K. Arthur and R. Deborah, “Biological Aspects of Endemism in Higher Plants,” Annual Review of Ecology and Systematics, Vol. 16, 1985, pp. 447-479. doi:10.1146/

[50]   T. A. Ranker, “Evolution of High Genetic Variability in the Rare Hawaiian Fern Adenophorus periens and Implications for Conservation Management,” Biological Conservation, Vol. 70, No. 1, 1994, pp. 19-24. doi:10.1016/0006-3207(94)90294-1

[51]   P. A. Martinez, L. E. Eguiarte and G. R. Furnier, “Genetic Diversity of the Endangered Endemic Agave victoriae- reginae (Agavaceae) in the Chihuahuan Desert,” American Journal of Botany, Vol. 86, No. 8, 1999, pp. 1093-1098. doi:10.2307/2656971

[52]   A. I. Mateu and M. G. Segura, “Population Subdivision and Genetic Diversity in Two Narrow Endemics of Antirrhinum L,” Molecular Ecology, Vol. 9, No. 12, 2000, pp. 2081-2087. doi:10.1046/j.1365-294X.2000.01119.x

[53]   A. Young, T. Boyle and T. Brown, “The Population Genetic Consequences of Habitat Fragmentation for Plants,” Trends in Ecology and Evolution, Vol. 11, No. 10, 1996, pp. 413-418. doi:10.1016/0169-5347(96)10045-8