Dolichos lablab Linn, newly renamed as Lablab purpureus (L) is herbaceous in nature, climbing type of growth habit, annual or perennial (for a short period of time) and possesses vigorous taproot. In the erect type, its thick stem can grow up to 3 feet, and the climbing type can grow up to 25 ft  . Botanically, it has trifoliate and long-stemmed leaves. Each egg-shaped leaflet widens in the middle and is 7.5 - 15 cm long. The flowers are positioned in plant in clusters on an un-branched inflorescence in the angle between the leaf and the main stem. The petal color may be white, blue, or purple flowers depending on variety. Seedpods are 4 - 5 cm to 10 cm long   , smooth, flat, pointed, and contain 2 to 4 seeds. Seeds can be white, cream, pale brown, dark brown, red, black, or mottled depending on variety.
Dolichos lablab L. is grown as both pulse and vegetable crop. As a pulse crop (crop harvested for dry seed), it is cultivated in Africa, Asia, and the Caribbean. The green pod is consumed for vegetable purpose. Dolichos lablab is also used as forage, hay, and silage purpose. This crop is also recognized as forage crop. The leaf is very palatable in nature but the stem is not. The seeds are moderately palatable. Overall, it is one of the most palatable legumes for animals  . The leaf protein content varies from 21% to 38% and the seed protein profile varies from 20% to 28% crude protein  . The leaves are excellent hay for cattle and goats. Dolichos lablab is also recognized as a nitrogen-fixing green manure crop to improve soil quality. It produces more dry matter than cowpea (Vigna unguiculata) during drought weather and can contribute around 1750 lb of leaf matter  or 2.5 tons of total biomass per acre  . Each ton of biomass again also produces 50 lb of nitrogen  . Its initial growth in some agroclimatic zone may be slow, but once established, it competes well with weeds. It has an extensive root system that again improves the physical condition as well as function of the soil. In the United States, Dolichos lablab is used as an ornamental plant especially in the cut flower industry  . It was reported that up to 55 cut stems per plant could be done depending on the weather condition  . Dolichos lablab is an old world food crop that is thought to have originated in Africa  or India  . Dolichos lablab can be grown in a variety of soils, from sandy to clay type of soils and in a pH range of 4.5 - 7.5  . It performs very poor in saline or poorly-drained soils, but it grows better than most legumes under acidic conditions  . It can grow in drought or shady conditions, and will grow in areas with an average annual rainfall is 25 - 120 in  . It is more drought resistant than other similar legumes like common beans (Phaseolus vulgaris) and cowpea  .
The main limiting problem in Dolichos lablab is that it is highly infested by aphid and consequently there is no fruitful harvest in favourable environmental condition. Aphid infestation is a major problem in many crops like Mustard, Bringal, Cotton, Cowpea etc. It was reported that a number of aphids (Hemiptera: Aphididae) are agriculturally important pests in a number of agricultural as well as horticultural crops. A number of disease causing viruses are also vectored by aphids in many crops like Cotton, Okra, Tomato and Chili etc. Aphid proliferates very quickly within very short period of time to achieve the population size above the economic threshold level. Aphid is exclusively phloem sap feeder. The mouth part of aphid is composed of the slender stylet and is very suitable for tapping the nutrient rich sieve element sap  . It is reported that the navigation of aphid stylet follows a predominantly intercellular route to minimize the cell damage that reduces the plant defense response on their way to reach the sieve element cell in the vascular tissue  .
Saliva is secreted during the aphid stylet navigation towards the sieve element and plays a vital role in successful penetration   . The first type of saliva is known as gel saliva and another type is also known as watery saliva  . The gelling saliva is proteinaceous in nature and encases the stylet after solidifying. This saliva is also known as sheath saliva and minimizes the physiological contact that reduces the induction of the plant defense response. After successful intercellular probing, the aphid secretes watery saliva into the host cell  . In this stage, switching from gel salivation to non-gelling (watery) salivation occurs into the penetrated cell or sieve element   . It is well documented that the watery saliva contains various enzymes like pectinases, cellulases, polyphenol oxidases, peroxidases, and lipases   . These enzymes have a role in establishing successful stylet penetration and subsequent prolong phloem sap feeding  .
High pressure is maintained in the vascular system in the higher plant. The vascular sap is rich in sugar and protein which makes it an attractive target by pathogens including aphid. Plant has effective mechanism to minimize the loss of sieve element sap. Stylet penetration causes the turgor shift and pressure loss  . The induced turgor shift accumulates intra sieve element calcium ion as well as changes the redox state. The higher accumulated Ca++ ion as well as turgor pressure shift are sensed by plant cell machinery which rapidly plugs the sieve pores to avoid the loss of sieve-tube sap  . The process is known as “Sieve-tube occlusion” which is regarded as instant phloem based plant defense in response to aphid attack  . The protein involved in sieve tube occlusion is well characterized. In Fabaceae family, the protein known as P-protein is responsible for sieve tube occlusion in a very fast way. This mechanism is universal in plants of Fabaceae family. Being a member of Fabaceae family, Dolichos lablab has sieve element occlusion mechanism. The phloem specific proteins (P-proteins) based occlusion occurs in some seconds in the Fabaceae family plants. The insoluble form of the P-proteins in sieve element present in the form like amorphous, granular, fibrillar, filamentous, tubular, and crystalline  , but the type of form is dependent on plant species  . It was well reported in V. faba that utilizes the “forisome dispersion” mechanisms (P-protein based occlusion) to avoid the phloem sap immediately. Therefore, plant evolved very fast mechanism with immediate effect that plays a universal safety design module  . In the present study, two popular varieties of Dolichos lablab were considered for aphid resistance study. Aphid colony establishment in vine was focused for better understanding the resistance mechanism between the two varieties. Both varieties are popular in West Bengal but one is more susceptible as compared to the other. In the present research, some plant specific parameters were considered for the differential response to aphid colony establishment especially in vine region.
2. Result and Discussion
2.1. Genotype Abbreviation and Description
The genotypes selected for aphid colony establishment had different characteristic features which are presented in Supplementary Figure 1. The flower petal colour of one genotype was pink and abbreviated as “susceptible” genotype in this manuscript and the white/light cream colour petal was the characteristic feature of “resistant” genotype (Supplementary Figure 1(a)). The flatten fruit which was considered as vegetable is higher in length as well as breath (Supplementary Figure 1(b)). The pale green color was the characteristic feature of susceptible variety as compared to resistance variety where the fruit color was more deep green (Supplementary Figure 1(b)). The adaxial side of leaflet was deep green in resistant variety as compared to susceptible variety where the adaxial leaflet color was pale green (Supplementary Figure 1(d)). The leaflet color in the abaxial side in both varieties was the same and it was pale green (Supplementary Figure 1(c)).
2.2. Plant Parts Which Were Prone to Aphid Population Establishment
Aphid colony establishment was not found in all the plant parts of the Dolichos lablab L. Some of plant parts were highly susceptible for aphid population establishment like vine (Supplementary Figure 2(a), Supplementary Figure 2(b)), leaf petiole (Supplementary Figure 2(d)), leaflet veins (Supplementary Figure 2(c)), immature fruit, flower etc. So it was better approach to monitor the differential aphid population establishment in those susceptible targets for better understanding of plant defense response.
2.3. Differential Response to Aphid Colony Establishment in Vines
The aphid infested vines from both the varieties were randomly selected at the early aphid infestation stage. This initiation stage was chosen because both “antibiosis” and “antixenosis” were effective and both the mechanisms gave lower number of aphid counts. The “antibiosis” mechanism is functional when the aphid metabolic activity is modulated by the plant defense response and ultimately affects on aphid fecundity. Another mechanism is “antixenosis” that strongly gives non-preference and plant does not act as an excellent host plant. During the early infestation, aphid will search alternative host if aphid faces extreme “antixenosis” from the present plant. This aphid population difference is significantly prominent in the early period of aphid infestation time (Figure 1). The 30 cm vine length from the tip of the vine is considered for aphid counting. It is observed that total number of aphids were significantly different in both the genotypes (p = 0.00). Based on aphid number (Figure 1(a)), the genotypes were classified as “resistant” as well as “susceptible” variety. The result shows that total aphid count was 215.5 ± 16.9 in susceptible variety, where as the aphid number in resistant variety at the same time was 48.2 ± 2.2. The mean number of aphids in both the genotypes were highly significant (p = 0.00). The same data is further analyzed in such a way that the number of aphids is considered below the mean number (215 ± 16.9) in the susceptible variety and the number of aphids is considered above the mean number (48 ± 2.2) in the resistant variety. After analysis, a significant difference was found in mean number of aphids in both the genotypes (p = 0.00). The mean aphid number in susceptible variety was 134 ± 6.5 and in resistant variety: 58 ± 3.6 (Figure 1(b)).
2.4. Plant Specific Factors That Modulate Aphid Colony Proliferation
2.4.1. Vine Diameter
Characteristically, it was recorded that the average vine diameter of susceptible variety was significantly higher as compared to resistant variety (p value = 0.00, Figure 2(a)). The average diameter of susceptible vine was 2.29 ± 0.094 mm where as the average diameter of resistant vine was 1.22 ± 0.047 mm. Due to increase in vine diameter, the distance from epidermis to the phloem cell was also higher (data not shown). The stylet length of a particular aphid is the characteristic feature of that aphid species. The fitness of aphid stylet length to reach into the phloem may be optimum for sucking the sieve element sap from phloem tissue in the higher diameter vine. Another significant observation was that the intercellular space between mesophyll cells was also higher in higher diameter vine. Aphid can explore the intercellular space during stylet penetration to avoid
Figure 1. Differential Genotypic response to aphid colony establishment in both vines. The aphid colony establishment in vine was measured in term of aphid number. (a) The differential aphid colony establishment was found on both the vine (total length considered is 30 cm from the tip of vine). The result shows that total aphid numbers are 215.5 ± 16.9 where as the aphid numbers in resistant variety are 48.2 ± 2.2. The mean numbers of aphid in both genotypes are highly significant (p = 0.00). (b) The aphid numbers above the mean value, 48.2 ± 2.2 in resistant variety and below the mean aphid numbers, 215.5 ± 16.9 in the susceptible variety were considered for further calculation. The selective data again shows that significant difference in aphid numbers in both genotypes realized (mean value in susceptible variety-58 ± 3.6 and resistant variety-134 ± 6.5, p = 0.00).
the cell rupture because the stylet penetration of aphid is intercellular that bypasses the plant defense response to sucking insect  . It was also observed that the phloem area as well as sieve element cell volume were also increased due to more vine diameter (data not shown). This higher diameter specifically facilitates and have promoting role in sieve element sap ingestion, which gives more proliferation in aphid population.
2.4.2. Wet/Dry Weight Ratio of Vine
Characteristically it was found that the ratio of wet weight by dry weight was higher in susceptible vines as compared to resistant vines (p value = 0.00, Figure 2(b)). This parameter indicated that susceptible vines had more water portion as compared to resistant vine. Anatomically, the plant vine is composed of various types of cell including epidermis and endodermis. The vascular system like xylem and phloem is the characteristic feature of the higher plants in both monocots and dicots. The phloem functions as a resource distribution and signaling conduit and the xylem functions as water as well as mineral translocator from root to shoot. More watery portion in susceptible vine indicates more sap in the vascular system and aphid are more assured to tap the phloem sap continuously. This higher feeding promotes aphid proliferation in the vine.
2.4.3 The Vine Growth Rate
Aphid population density was higher in susceptible vines as compared to resistant vines (Figure 1(a)), where as the growth rate was significantly higher in susceptible vine as compared to resistant vine (Figure 2(c)). The vine growth in 3 days was 13.74 ± 0.84 cm and growth rate was 4.6 ± 0.28 cm/day in resistant variety where as the vine growth in 3 days was 23.69 ± 1.45 cm and growth rate was 7.9 ± 0.48 cm/day in susceptible variety. The growth rate of vine in susceptible variety as compared to resistant variety was 1.725 times more in a day. So the actual mean number of aphids in susceptible variety was higher than the mean aphid numbers (215.5 ± 16.9) in the susceptible variety.
2.4.4. Phloem Sap Pressure in Both Varieties
Another interesting feature of phloem is that phloem sap is maintained with high pressure. One mode of tapping phloem sap is the passive mode and this mode is explored to suck the sap due to higher pressure maintained in phloem tissue. After establishing the stylet penetration with the action of both gel and watery saliva, aphid may enjoy the passive drinking. For measurement of the high pressure, the total ooze out sap from vine was collected and measured. The total volume of ooze out per mm2 was indicator for pressure existing in the vascular system. The more ooze out volume per mm2 would indicate more pressure being maintained in the vascular system. The data showed that the ooze out volume in susceptible variety was 0.68 ± 0.11 µl per mm2 where as the ooze out volume in resistant vine is 0.19 ± 0.10 (Figure 3(a)). The ooze out volume per mm2 in susceptible variety was 3.6 times the pressure maintained in the resistant variety vine. This data indicated that higher pressure was maintained in susceptible
Figure 2. Plant vine characteristics of both resistant as well as susceptible varieties. (a) Average diameter of vines of both genotypes. The average diameter of three positions (5, 15, 25 cm from the tip of the vine) of the 30 cm length vine were considered for calculation. (b) Wet/dry weight ratio of 30 cm vine. (c) Growth rate of both resistant and susceptible varieties in 3 days. The growth rate @ 4.6 ± 0.28 cm/day in resistant variety and @7.9 ± 0.48 cm/day in susceptible variety were recorded.
variety in the vascular system (Figure 3(a)) and the aphid explored this higher pressure for passive ingestion and maintained well feeding.
2.4.5. Vine Compactness Measurement
The average pressures to penetrate the metallic probe to 0.8 mm of three points (5, 15. 25 cm from the tip of vine) were considered for calculation. The maximum pressure required for penetrating 0.8 mm was 69.07 ± 2.29 g (Figure 4) in resistant vine where as the maximum pressure required for penetrating 0.8 mm was 63.43 ± 1.49 g (Figure 4). Aphid penetrates its slender stylet towards the sieve element. The toughness was higher in resistant variety vines as compared to susceptible vines (p = 0.0361, Figure 4). It is easier to penetrate the slender stylet in susceptible vines. The toughness was also significantly higher in different parts of intra-vines of both genotypes as well as inter-vines (Supplementary Figure 3(a) and Supplementary Figure 3(b)).
2.5. Impact of Some Characteristic Features on Overall Aphid Number in Both Genotypes
It was found that some plant specific features promoted the aphid number in susceptible genotype. Aphid did not colonize the entire plant, rather specific
Figure 3. Phloem sap pressure in both varieties. (a) Sap Ooze-out volume per mm2 (in 15s). The ooze out volume coming from vine in susceptible variety is 0.68 ± 0.11 µl per mm2 where as the ooze out volume coming from vine in resistant variety is 0.19 ± 0.10. The ooze out volume per mm2 in susceptible variety was 3.6 times more than the resistant variety vine. (b) Vine diameter in mm. The vines considered for sap ooze-out volume were also considered for diameter measurement. The data indicated that vines from both the genotypes were significantly different (p = 0.0001).
Figure 4. The maximum pressure required to penetrate 0.8 mm of the pointed probe under constant pressure (5 g). Average maximum pressure required to penetrate the pointed probe in both vines of susceptible as well as resistant varieties. The p value indicates the significant difference for the maximum pressure required.
parts like vine, leaf petiole, leaflet veins at the base portion, inflorescence, immature fruits etc. (Supplementary Figure 2). The same sized leaflet (Figure 5(b)) was fleshier as compared to resistant variety leaflet (Figure 5(a), p = 0.0255). This also ensured more aphid proliferation in this leaf and affected accumulative aphid number in the plant. From the leaflet wilting experiment, it was found that the turgor pressure was more in susceptible leaflet as compared to resistant leaflet (Figure 5(c)). In this experiment, leaflet of same size (Figure 5(b)) were collected and kept on 2 ml centrifuge tube for 10 minutes without water or any solution. Within the susceptible genotype, the variation of leaflet size also existed. On consideration of larger sized leaflet, it was found that the ratio of wet/dry weight was also significantly higher (Supplementary Figure 4(a) and p = 0.0001) and had the significant difference in diameter at the base of the respective leaflet (Supplementary Figure 4(b) and p = 0.00), that promoted aphid colony proliferation. Further fine tuning of the larger sized leaflet, it was
Figure 5. Some characteristic features which had an impact on aphid number variation in both the genotypes. (a) Ratio of Wet/Dry Weight in medium size leaflet. (b) Leaflet vein Length (cm). (c) Leaflet drooping experiment shows that susceptible leaflet was more to wilt as compared to resistant leaflet.
found that the relative distribution of aphid colony proliferation within larger size leaflet was also significantly different (Supplementary Figure 4(c) and p = 0.0001) and it was correlated with the diameter of the leaflet vein diameter (Supplementary Figure 4(d) and p = 0.0001).
2.6. Plant Specific Factors That Had an Impact on Aphid Specific Parameter
The argument may arise that significantly higher number of aphids in susceptible vine was due to initial inoculums, not from the true proliferation. Aphid generally does not move from one plant to another plant but the alate form moves from one plant to another but it would be produced in particular environment. To address the argument, initial infestation phase was chosen when there was no competition of space among aphids for getting enough phloem sap. The immature to mature stages was chosen for fecundity count. If the aphid was well fed, it showed high fecundity, otherwise it showed lower fecundity. In the resistant vine, the ratio of instars 1 - 4 to instars 5 i.e. immature aphid to adult aphid was significantly lower than the susceptible vine (Figure 6(a)). The mean number of progenies per adult was 0.89 ± 0.12 in resistant vine whereas the mean number of progenies per adult was 2.68 ± 0.35 in susceptible vine (Figure 6(a) and p = 0.00). Moreover, the wet/dry weight ratio of aphids collected from vine was also significantly higher (Figure 6(b), p = 0.0378) in susceptible vine (mean value = 2.646 ± 0.037) as compared to resistant vine (mean value =
Figure 6. Aphid characteristics grown in both Resistant as well as Susceptible variety. (a) The ratio of aphid stages belong to Instars 1 - 4 to Instars 5 (Immature aphid per adult aphid). The mean number of progenies per adult aphid is 0.89 ± 0.12 in resistant vine (30 cm length from the tip of the vine) where as the mean number of progenies per adult aphid is 2.68 ± 0.35 in susceptible vine. (b) The wet weight to dry weight ratio of aphid grown both in resistant and susceptible varieties. The wet/dry weight ratio of aphids collected from vine is also significantly higher (p = 0.0378) in susceptible vine grown aphid (mean value = 2.646 ± 0.037) as compared to resistant vine grown aphid (mean value = 2.5411 ± 0.026).
2.5411 ± 0.026). The higher watery portion in aphid was indicative of well feeding of sieve element sap from the susceptible vine as compared to resistant vine. The well feeding promotes more aphid proliferation in susceptible vine and results in higher fecundity.
The results discussed above indicated that aphid proliferation was significantly higher in susceptible vine. Higher aphid population may arise due to more initial harboring of aphid on susceptible vine. But the aphid (entomologically belonging to nymph stage), rarely moved to another plant when isolation distance is more than 4 meters. Moreover, aphids were well fed in susceptible vine as compared to resistant vine because the more water content in aphid was found in susceptible vine. The more phloem sap ingestion may promote more aphid proliferation in susceptible vine as compared to resistant vine and ultimately contribute more progenies per adult aphid in susceptible vine. Aphid facing lesser water content in resistant vine, definitely faces limitation to ingest sufficient volume of phloem sap and/or there was antibiotic effect of phloem sap that discourages aphid fecundity. Some botanic factors like higher diameter, more water content of vine and leaflet, higher turgor pressure in the vascular system, and less compactness of vine in the susceptible variety ensured positively assured well feeding by aphid and more water content ensured more aphid fecundity. The accumulative and/or synergistic effect in susceptible vine derived facilitations, aphid proliferated at a faster rate and ultimately gave more aphid number in the susceptible vine.
3. Material and Methods
3.1. Plant Material Related Information
Dolichos lablab genotype which was collected from Beldanga, Murshidabad, West Bengal, India was the “susceptible” variety. Another Dolichos lablab genotype collected from Pundibari, Cooch Behar, North West Bengal, India was the “resistant” one.
3.2. Plant Growth Management
Both the varieties were sown on October 15, 2015 in Terai zone, Pundibari, Cooch Behar, West Bengal, India. The plant growth was maintained in Terai zone (Pundibari, Cooch Behar) in the UBKV campus. The plant canopy was maintained in iron structure and the dimension of the iron structure was 2.4 m × 1.84 m × 0.8 m (Length × Breath × Height). Irrigation is given as required. No fertilizer was given.
3.3. Weather Information
The experimental plot belonged to the “Terai” zone that is positioned in south of the outer Himalayan foothills. The average rainfall in the month of October, November and December was 1.49 ± 1.02, 0.46 ± 0.39 and 0.17 ± 0.12 mm, respectively. The maximum and minimum temperature realized was 32.19˚C ± 0.28˚C and 20.45˚C ± 0.38˚C in October 2015, 29.13˚C ± 0.23˚C and 15.27˚C ± 0.40˚C in November 2015, and 25.03˚C ± 0.33˚C and 10.45˚C ± 0.62˚C in December 2015. The maximum and minimum relative humidity (RH) was 80.55˚C ± 1.02˚C and 74.39˚C ± 1.57˚C in October 2015, 82.43˚C ± 1.23˚C and 83.77˚C ± 0.90˚C in November 2015 and 89.84˚C ± 0.93˚C and 85.23˚C ± 1.02˚C in December 2015.
3.4. Soil Information
Soil of the Terai region is mostly acidic in reaction and contains high amounts of Fe and Al oxides and hydroxides. Soils of the Pundibari experimental plots were slightly acidic (pH 5.5 to 6.4) and sandy loam in texture with low status of the available N, P2O5, and K2O (211, 11.4, and 95 kg/ha, respectively  . The content of S (33.7 kg/ha) and Zn (1.25 kg/ha) was quite high in terms of the critical limit, while extractable B (0.28 kg/ha) was low in these experimental soils.
The plant canopy was maintained under natural environment in terai zone. No protection measure was followed for controlling aphid infestation. Aphid infestation occurred naturally and data was taken at the early infestation stage. When the aphid infestation occurred naturally, data was taken at the early infestation stage. The aphid data was taken at 52 days old plant.
3.6. Diameter Measurement
Digimatic Caliper (Mitutoyo Corporation) was used for measuring the diameter of the plant vine in absolute mode i.e. in mm.
3.6.1. Dry Weight and Wet Weight Measurement
A vine of length 30 cm was considered for taking the fresh weight after immediate collection. After taking the total weight data, the vine was incubated at 72˚C for 3 days for drying. After drying, the dry weight was measured. The wet weight was calculated by subtracting dry weight from fresh weight. The wet weight and dry weight ratio was considered for further scientific explanation.
3.6.2. Turgor Pressure Measurement
The turgor pressure was measured in terms of phloem sap volume per unit area. The vine was cut at 15 cm from the tip and allowed for 15 seconds to measure the ooze out volume which was accumulated due to high pressure maintained in phloem. The ooze out volume measurement was taken with the help of 10 µl pipette. The volume per unit area of vine was considered for this study. The data was taken at 2 pm.
3.7. Vine Growth Rate of Both Resistant and Susceptible Varieties in 3 Days
The vine WAS marked at 30 cm position from the tip of the respective varieties. After 3 days, the total vine length WAS again measured. The vine length increment per day was considered for scientific explanation.
3.8. Probe Penetration Experiment
The probe penetration experiment was performed by the TA.XT plus Texture Analyser (Stable Micro Systems). Aphid penetrated its slender stylet into vine to reach into phloem region to tap the nutrient enriched sap. During probe penetration, aphid has to overcome the tissue resistance from plant vine. The probe penetration experiment was performed to measure the resistance of both plant vines. Both vines were in different diameter and the penetration length was considered to be 0.8 mm. During probe penetration, the resistance faced by probe was measured with constant pressure of 5 g. The probe considered for penetration experiment was 2 mm pointed needle. The resistance was measured in different parts of vines at 5, 15, and 25 cm from the tip of the vine. The average pressures of three points of both vines were considered for calculation.
3.9. Statistical Analysis
All the statistical calculation was performed by using the Minitab 15 Statistical Software. Comparison between two sets of data was carried out by General Linear Model.
The authors are thankful to the Head, Department of Genetics and Plant Breeding, UBKV for providing all the infrastructural help. The authors are also thankful to Central Instrumentation Centre (CIC), UBKV for taking Probe penetration experiment and providing access to instrument for taking weight data. The authors are thankful to “Gramin Krishi Mousam Sewa (IAAS)” for providing the weather data. The technical assistance from technical person from CIC, UBKV is sincerely acknowledged.
Supplementary Figure (SF). 1. The characteristic features of flower and fruit in both varieties (R-Resistance, S-Susceptible).
Supplementary Figure 1.(a) The difference in petal color in the both varieties. The Left one is Resistant variety flower and the right one is Susceptible variety flower. (b) The fruit characteristic of both the varieties. The fruit size of susceptible variety was higher than resistant variety. (c) The abaxial side of leaf in both the varieties were pale green in color. (d) The adaxial side of leaf in both varieties but the leaf color in resistant variety was deep green.
Supplementary Figure 2.Plant parts prone to aphid population establishment. (All aphid infested plant parts are taken from susceptible genotype). (a) The canopy of susceptible genotype maintained in iron structure. (b) The vine of 30 cm is highly susceptible for aphid colony establishment. Within figure, the left vine is 10 cm from tip of the vine, middle is next 10 cm from the 1st vine, and right one is 10 cm vine from 2nd vine. On all parts of vine aphid population was established. (c) The leaf veins but at the base of the leaflets are prone to aphid population establishment. (d) The leaf petiole is another target for aphid population establishment.
Supplementary Figure 3.(a) Pressure required to penetrate 0.8 mm in both vines in different points (5, 15, 25 cm from the tip of the respective vines from both genotypes). R1, R2 and R3 indicate the point of penetration at 5, 15 and 25 cm from the tip of the resistant vine. S1, S2 and S3 R3 indicate the point of penetration at 5, 15 and 25 cm from the tip of the susceptible vine. (b) The table for p values.
Supplementary Figure 4.Some parameters that promote aphid number in the susceptible variety. (a) Characteristic feature of medium as well as larger sized leaflet. Ratio of Wet/Dry Weight of medium and larger sized leaflet within Susceptible Variety. (b) The vein diameter in mm at the base of leaflet in both medium and larger sized leaflets within susceptible variety. This signified that larger sized leaflet within same genotype gives more leaflet diameter at the base. (c) The relative distribution of aphid within different parts of the larger sized leaflet. (d) The diameter at the base as well as middle of the larger sized leaflet. Characteristic feature within the larger sized leaflet.
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