AiM  Vol.7 No.2 , February 2017
Inhibitory Activity of Burkholderia sp. Isolated from Soil in Gotsu City, Shimane, against Magnaporthe oryzae
Abstract: An isolate GT4028 was obtained from soil samples collected from a field in Gotsu city (Kawahira), Shimane. The use of a culture suspension and culture filtrate of this isolate significantly suppressed the spore germination in Magnaporthe oryzae. The inhibitory activity of the culture filtrate was heat-stable. The formation of rice blast lesions by M. oryzae was significantly suppressed in the presence of the culture suspension of isolate GT4028. Furthermore, mycelial growth of some plant pathogenic fungi was inhibited by the isolate in a dual culture assay. Sequence analysis of 16S rDNA region of the isolate indicated that it shared similarities with species of the genus Burkholderia. Also, isolate GT4028 could be grown even in the presence of fungicides (Blastin, Kasugamycin, and Amistar) that act against M. oryzae. These results suggest that isolate GT4028 might be a potential control agent for plant protection against diseases, such as rice blast disease.
Cite this paper: Lemtukei, D. , Tamura, T. , Nguyen, Q. and Ueno, M. (2017) Inhibitory Activity of Burkholderia sp. Isolated from Soil in Gotsu City, Shimane, against Magnaporthe oryzae. Advances in Microbiology, 7, 137-148. doi: 10.4236/aim.2017.72011.

[1]   Dean, R., Van Kan, J.A., Pretorius, Z.A., Hammond-Kosach, K.E., Di Pietro, A., Spanu, P.D., Rudd, J.J., Dickman, M., Kahmann, R., Ellis, J. and Foster, G.D. (2012) The Top 10 Fungal Pathogens in Molecular Plant Pathology. Molecular Plant Pathology, 13, 414-430.

[2]   Liu, J., Wang, X., Mitchell, T., Hu, Y., Liu, X., Dai, L. and Wang, G.L. (2010) Recent Progress and Understanding of the Molecular Mechanisms of the Rice-Magnaporthe oryzae Interaction. Molecular Plant Pathology, 11, 419-427.

[3]   Ishii, H. (2006) Impact of Fungicide Resistance in Plant Pathogens on Crop Disease Control and Agricultural Environment. Japan Agricultural Research Quarterly, 40, 205-211.

[4]   Elshafie, H.S., Camele, I., Racioppi, R., Scrano, L., Iacobellis, N.S. and Bufo, S.A. (2012) In Vitro Antifungal Activity of Burkholderia gladioli pv. agaricicola against Some Phytopathogenic Fungi. International Journal of Molecular Sciences, 13, 16291-16302.

[5]   Lemtukei, D., Tamura, T., Nguyen, T.Q., Kihara, J. and Ueno, M. (2016) Antagonistic Potential of Isolated Microorganisms from Soil in Shimane Prefecture against Rice Blast Disease Cause by Magnaporthe oryzae. Bulletin of the Faculty of Life and Environmental Science, Shimane University, 21, 9-12.

[6]   Huong, N.L., Itoh, K. and Suyama, K. (2007) Diversity of 2,4-Dichlorophenoxyacetic Acid (2,4-D) and 2,4,5-Trichlorophenoxyacetic Acid (2,4,5-T)-Degrading Bacteria in Vietnamese Soils. Microbes and Environments, 22, 243-256.

[7]   Matsui, T., Kato, K., Namihira, T., Shinzato, N. and Semba, H. (2009) Stereospecific Degradation of Phenylsuccinate by Actinomycetes. Chemosphere, 76, 1278-1282.

[8]   Suzuki, S., Taketani, H., Kusumoto, K. and Kashiwagi, Y. (2006) High-Throughput Genotyping of Filamentous Fungus Aspergillus oryzae Based on Colony Direct Polymerase Chain Reaction. Journal of Bioscience and Bioengineering, 102, 572-574.

[9]   Saitou, N. and Nei, M. (1987) The Neighbor-Joining Method: A New Method for Reconstructing Phylogenetic Trees. Molecular Biology and Evolution, 4, 406-425.

[10]   Nguyen, Q.T., Ueda, K., Kihara, J., Arase, S. and Ueno, M. (2012) Effect of Culture Filtrates of Trichoderma sp. Isolated from Wild Mushrooms on the Infectious Behavior of Plant Pathogenic Fungi. Bulletin of the Faculty of Life and Environmental Science, Shimane University, 17, 23-27.

[11]   Coenye, T. and Vandamme, P. (2003) Diversity and Significance of Burkholderia species Occupying Diverse Ecological Niches. Environmental Microbiology, 5, 719-729.

[12]   McLoughlin, T.J., Quinn, J.P., Bettermann, A. and Bookland, R. (1992) Pseudomonas cepacia Suppression of Sunflower Wilt Fungus and Role of Antifungal Compounds in Controlling the Disease. Applied and Environmental Microbiology, 58, 1760-1763.

[13]   Mao, S., Lee, S.J., Hwangbo, H., Kim, Y.W., Park, K.H., Cha, G.S., Park, R.D. and Kim, K.Y. (2006) Isolation and Characterization of Antifungal Substances from Burkholderia sp. Culture Broth. Current Microbiology, 53, 358-364.

[14]   Kang, J.G., Shin, S.Y., Kim, M.J., Bajpai, V., Maheshwari, D.K. and Kang, S.C. (2004) Isolation and Anti-fungal Activities of 2-Hydroxymethyl-Chroman-4-One Produced by Burkholderia sp. MSSP. The Journal of Antibiotics, 57, 726-731.

[15]   Tawfik, K.A., Jeffs, P., Bray, B., Dubay, G., Falkinham, J.O., Mostafa, M., Youssef, D., Khalifa, S. and Schmidt, E.W. (2010) Burkholdines 1097 and 1229, Potent Antifungal Peptides from Burkholderia ambifaria 2.2N. Organic Letters, 12, 664-666.

[16]   Lin, Z., Falkinham, J.O., Tawfik, K.A., Jeffs, P., Bray, B., Dubay, G., Cox, J.E. and Schmidt, E.W. (2012) Burkholdines from Burkholderia ambifaria: Antifungal agents and Possible Virulence Factors. Journal of Natural Products, 75, 1518-1523.

[17]   Lu, S., Novak, J., Austin, F.W., Gu, G., Ellis, D., Kirk, M., Wilson-Stanford, S., Tonelli, M. and Smith, L. (2009) Occidiofungin, a Unique Antifungal Glycopeptide Produced by a Strain of Burkholderia contaminans. Biochemistry, 48, 8312-8321.

[18]   Joo, G.J., Kang, S.M., Hamayun, M., Kim, S.K., Na, C.I., Shin, D.H. and Lee, I.J. (2009) Burkholderia sp. KCTC 11096BP as a Newly Isolated Gibberellin Producing Bacterium. The Journal of Microbiology, 47, 167-171.

[19]   Scuderi, G., Bonaccorsi, A., Panebianco, S., Vitale, A., Polizzi, G. and Cirvilleri, G. (2009) Some Strain of Burkholderia gladioli are Potential Candidates for Postharvest Biocontrol of Fungal Rots in Citrus and Apple Fruits. Journal of Plant Pathology, 91, 207-213.