JEP  Vol.2 No.7 , September 2011
Mineralization of Petroleum Contaminated Wastewater by Co-Culture of Petroleum-Degrading Bacterial Community and Biosurfactant-Producing Bacterium
Abstract: Activity of a crude biosurfactant extracted from the culture fluid of Serratia sp. that was isolated from riverbed soil was shown to increase in proportion to the cultivation time, and was higher at pH 8 than at pH 7. Serratia sp. grew in the mineral-based medium with soybean oil but was not with kerosene-diesel. The petroleum-degrading bacteria—Acinetobacter sp., Pseudomonas sp., Paracoccus sp., and Cupriavidus sp.—were isolated from a specially designed enrichment culture. The efficiency of mineralization of wastewater contaminated with kerosene and diesel (WKD) by the petroleum-degrading bacterial community (PDBC) was enhanced significantly by addition of the crude biosurfactant. The efficiency of mineralization of the WKD was also about 2 times boosted by co-culture of Serratia sp. and PDBC. Bacterial community of Serratia sp. and PDBC co-cultivated in the WKD was maintained for at least 8 days according to the TGGE pattern of 16S rDNA obtained from the bacterial culture. In conclusion, the co-culture of Serratia sp. and PDBC is an applicable technique for the mineralization of wastewater contaminated with petroleum, which may substitute for chemical or biological surfactant.
Cite this paper: nullB. Jeon, I. Jung and D. Park, "Mineralization of Petroleum Contaminated Wastewater by Co-Culture of Petroleum-Degrading Bacterial Community and Biosurfactant-Producing Bacterium," Journal of Environmental Protection, Vol. 2 No. 7, 2011, pp. 895-902. doi: 10.4236/jep.2011.27102.

[1]   J. P. Allen, E. A. Atekwana, J. W. Duris, D. D. Werkema and S. Rossbach, “The Microbial Community Structure in Petroleum-Contaminated Sediments Corresponds to Geophysical Signatures,” Applied Environmental Microbiology, Vol. 73, No. 9, 2007, pp. 2860-2870. doi:10.1128/AEM.01752-06

[2]   S. K. Haack and B. A. Bekins, “Microbial Populations in Contaminant Plumes,” Hydrogeology Journal, Vol. 8, No. 1, 2000, pp. 63-76. doi:10.1007/s100400050008

[3]   J. Y. Lee, J. Y. Cheon, K. K. Lee, S. Y. Lee and M. H. Lee, “Factors Affecting The Distribution Of Hydrocarbon Contaminants And Hydrogeochemical Parameters In A Shallow Sand Aquifer,” Journal of Contaminant Hydrology, Vol. 50, No. 1-2, 2001, pp. 139-158. doi:10.1016/S0169-7722(01)00101-2

[4]   W. A. Sauck, E. A. Atekwana and M. S. Hash, “High Conductivities Associated with an LNAPL Plume Imaged by Integrated Geophysical Techniques,” Journal of Environtal & Engineering Geophysics, Vol. 2, No. 3, 1998, pp. 203-212.

[5]   I. M. Banat, R. S. Makkar and S. S. Cameotra, “Potential Commercial Applications of Microbial Surfactants,” Applied Microbiology and Biotechnology, Vol. 53, No. 5, 2000, pp. 495-508. doi:10.1007/s002530051648

[6]   J. D. Desai and I. M. Banat, “Microbial Production of Surfactants and Their Commercial Potential,” Microbiology and Molecular Biology Review, Vol. 61, No. 1, 1997, pp. 47-64.

[7]   C. N. Mulligan, R. N. Yong and B. F. Gibbs, “On the Use of Biosurfactant for the Removal of Heavy Metals from Oil-Contaminated Soil,” Environmental Progress, Vol. 18, No. 1, 1999, pp. 50-54. doi:10.1002/ep.670180120

[8]   S. C. Lin, M. A. Minton, M. M. Sharma and G. Georgiou, “Structural and Immunological Characterization of a Biosurfactant Produced by Bacillus licheniformis JF-2,” Applied Environmental Microbiology, Vol. 60, No. 1, 1994, pp. 31-38.

[9]   M. J. McInerney, S. Maudgalya, D. P. Nagle and R. M. Knapp, “Properties of the Biosurfactant Produced by Bacillus licheniformis Strain JF-2,” Journal Industrial Microbiology, Vol. 5, No. 2-3, 1990, pp. 95-102. doi:10.1007/BF01573858

[10]   M. E. Davey, N. C. Caiazza and G. A. O’Toole, “Rhamnolipid Surfactant Production Affects Biofilm Architecture in Pseudomonas aeruginosa PAO1,” Journal of Bacteriology, Vol. 185, No. 3, 2003, pp. 1027-1036. doi:10.1128/JB.185.3.1027-1036.2003

[11]   S. S. Branda, J. E. Gonzalez-Pastor, S. Ben-Yehuda, R. Losick and R. Kolter, “Fruiting Body Formation by Bacillus subtilis,” Proceeding National Academy of Science USA, Vol. 98, No. 20, 2001, pp. 11621-11626. doi:10.1073/pnas.191384198

[12]   J. C. Fountain, A. Klimek, M. G. Beikirch and T. M. Middleton, “The Use of Surfactant for in Situ Extraction of Organic Pollutants from a Contaminated Aquifer,” Journal of Hazardous Materials, Vol. 28, No. 3, 1991, pp. 295-311. doi:10.1016/0304-3894(91)87081-C

[13]   J. W. Mercer and R. M. Cohen, “A Review of Immiscible Fluids in the Subsurface: Properties, Models, Characterization and Remediation,” Journal of Contaminant Hydrology, Vol. 6, No. 2, 1990, pp. 107-163. doi:10.1016/0169-7722(90)90043-G

[14]   S. A. Churchill, R. A. Griffin, P. P. Jones and P. F. Churchill, “Biodegradation Rate Enhancement of Hydrocarbons by an Oleophilic Fertilizer and a Rhamnolipid Biosurfactant,” Journal of Environmental Quality, Vol. 24, No. 1, 1995, pp. 19-28. doi:10.2134/jeq1995.00472425002400010003x

[15]   D. K. Jain, H. Lee and J. T. Trevors, “Effect of Addition of Pseudomonas aeruginosa UG2 Inocula or Biosurfactants on Biodegradation of Selected Hydrocarbons in Soil,” Journal of Industrial Microbiology, Vol. 10, No. 2, 1992, pp. 87-93. doi:10.1007/BF01583840

[16]   Y. Zhang and R. M. Miller, “Enhanced Octadecane Dispersion and Biodegradation by a Pseudomonas rhamnolipid Surfactant (Biosurfactant),” Applied Environmental Microbiology, Vol. 58, No. 10, 1992, pp. 3276-3282.

[17]   H. S. Kang, B. K. Na and D. H. Park, “Oxidation of Butane to Butanol Coupled to Electrochemical Redox Reaction of NAD+/NADH,” Biotechnology Letters, Vol. 29, No. 4, 2007, pp. 1277-1280. doi:10.1007/s10529-007-9385-7

[18]   W. J. Lee, J. K. Lee, J. Chung, Y. J. Cho and D. H. Park, “Effects of Electrochemical Reduction Reactions on the Biodegradation of Recalcitrant Organic Compounds (ROCs) and Bacterial Community Diversity,” Journal of Microbiology and Biotechnology, Vol. 20, No. 8, 2010, pp. 1230-1239. doi:10.4014/jmb.0910.10016

[19]   S. J. Lee, Y. W. Lee, J. Chung, J. K. Lee, J. Y. Lee, D. Jahng, Y. Cha and Y. Yu, “Reuse of Low Concentrated Electronic Wastewater Using Selected Microbe Immobilized Cell System,” Water Science and Technology, Vol. 57, No. 8, 2008, pp. 1191-1197. doi:10.2166/wst.2008.246

[20]   K. McClay, B. G. Fox and R. J. Steffan, “Toluene Monooxygenase-Catalyzed Expoxidation of Alkenes,” Applied Environmental Microbiology, Vol. 66, No. 5, 2000, pp.1877-1882. doi:10.1128/AEM.66.5.1877-1882.2000

[21]   C. E. Cerniglia, “Biodegradation of Polycyclic Aromatic Hydrocarbons,” Current Opinion in Biotechnology, Vol. 4, No. 3, 1993, pp. 331-338. doi:10.1016/0958-1669(93)90104-5

[22]   B. N. Aronstein and M. Alexander, “Effect of a Non-Ionic Surfactant Added to the Soil Surface on the Biodegradation of Aromatic Hydrocarbons within the Soil,” Applied Microbiology and Biotechnology, Vol. 39, No. 1, 1993, pp. 386-390.

[23]   E. Rosenberg, “Microbial Surfactants,” Critical Review in Biotechnology, Vol. 3, No. 2, 1986, pp. 109-132. doi:10.3109/07388558509150781

[24]   T. Barkay, S. Navon-Venezia, E. Z. Ron and E. Rosenberg, “Enhancement of Solubilization and Biodegradation of Polyaromatic Hydrocarbons by the Bioemulsifier Alasan,” Applied Environmental Microbiology, Vol. 65, No. 6, 1990, pp. 2697-2702.

[25]   S. Harvey, I. Elashvili, J. J. Valdes, D. Kamely and A. M. Chakrabarty, “Enhanced Removal of Exxon Valdez Spilled Oil from Alaskan Gravel by a Microbial Surfactant,” Biological Technology, Vol. 8, No. 3, 1990, pp. 228-230.

[26]   G. Bai, M. L. Brussenau and R. M. Miller, “Biosurfactant-Enhanced Removal of Residual Hydrocarbon from Soil,” Journal of Contaminant Hydrology, Vol. 25, No. 1-2, 1997, pp. 157-170. doi:10.1016/S0169-7722(96)00034-4

[27]   A. Oberbremer, R. Müller-Hurtig and F. Wagner, “Effect of the Addition of Microbial Surfactants on Hydrocarbon Degradation in a Soil Population in a Stirred Reactor,” Applied Microbiology and Biotechnology, Vol. 32, No. 4, 1990, pp.485-489. doi:10.1007/BF00903788

[28]   I. M. Banat, “Biosurfactants Production and Possible Uses in Microbial Enhanced Oil Recovery and Oil Pollution Remediation,” A review, Bioresource Technology, Vol. 51, No. 1, 1995, pp. 1-12. doi:10.1016/0960-8524(94)00101-6

[29]   Q. Li, C. Kang, H. Wang, C. Liu and C. Zhang, “Application of Microbial Enhanced Oil Recovery Technique to Daqing Oil Field,” Biochemical Engineering Journal, Vol. 11, No. 2-3, 2002, pp. 197-199. doi:10.1016/S1369-703X(02)00025-6