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 AiM  Vol.6 No.14 , December 2016
Comparative Analysis of Mycotoxigenic Fungi and Mycotoxins Contaminating Soya Bean Seeds and Processed Soya Bean from Nigerian Markets
Abstract:
Concern for food safety has continued to grow worldwide including the issue of mycotoxin contamination of food products from farm to fork. In this regard, soya bean seeds and processed soya bean powder bought from some Nigerian markets were screened for fungal and mycotoxin contamination. Fungal identification was done by both conventional and molecular methods after samples were cultured on potato dextrose agar (PDA), ohio agricultural experimental station agar (OAESA), malt extract agar (MEA) and czapek yeast agar (CYA). Mycotoxin analysis by thin layer chromatography and high performance liquid chromatography was done after extraction and clean-up by multi-mycotoxin extraction procedure and solid phase extraction (SPE) isolute strong ion exchange (SAX) columns. Results from the analysis showed that soya bean seeds had higher incidences of fungal species such as Alternaria (52.4%) and Aspergillus flavus (42.9%). Mycotoxins detected include aflatoxins, ochratoxin A and fumonisin B with highest concentration of 3.430 μg/g, 0.125 μg/g and 4.286 μg/g respectively, which were below regulatory limits. The study showed that there was co-occurrence of aflatoxins and fumonisin B1 in both sample types and though these values are low, should not be ignored as a result of health risks associated with exposure to these compounds.
Cite this paper: Egbuta, M. , Mwanza, M. , Phoku, J. , Chilaka, C. and Dutton, M. (2016) Comparative Analysis of Mycotoxigenic Fungi and Mycotoxins Contaminating Soya Bean Seeds and Processed Soya Bean from Nigerian Markets. Advances in Microbiology, 6, 1130-1139. doi: 10.4236/aim.2016.614102.
References

[1]   Hoffman, J.R. and Falvo, M.J. (2004) Protein—Which Is Best? Journal of Sports Science & Medicine, 3, 118-130.

[2]   Dugje, I.Y., Omoigui, L.O., Ekeleme, F., Bandyopadhyay, R., Kumar, L.P. and Kamara, A.Y. (2009) Farmers’ Guide to Soybean Production in Northern Nigeria. International Institute of Tropical Agriculture (IITA).

[3]   Shurtleff, W. and Aoyagi, A. (2007) History of Soy in Africa. Soyinfo Center, Lafayette.

[4]   Zain, M.E. (2011) Impact of Mycotoxins on Humans and Animals. Journal of Saudi Chemical Society, 15, 129-144.
https://doi.org/10.1016/j.jscs.2010.06.006

[5]   Wagacha, J.M. and Muthomi, J.W. (2008) Mycotoxin Problem in Africa: Current Status, Implications to Food Safety and Health and Possible Management Strategies. International Journal of Food Microbiology, 124, 1-12.
https://doi.org/10.1016/j.ijfoodmicro.2008.01.008

[6]   Ferrigo, D., Raiola, A. and Causin, R. (2016) Fusarium Toxins in Cereals: Occurrence, Legislation, Factors Promoting the Appearance and Their Management. Molecules, 21, 1-35.
https://doi.org/10.3390/molecules21050627

[7]   Atanda, S.A., Pessu, P.O., Agoda, S., Isong, I.U., Adekalu, O.A., Echendu, M.A. and Falade, T.C. (2011) Fungi and Mycotoxins in Stored Foods. African Journal of Microbiology Research, 5, 4373-4382.
https://doi.org/10.5897/ajmr11.487

[8]   Pereira, V.L., Fernandes, J.O. and Cunha, S.C. (2014) Mycotoxins in Cereals and Related Foodstuffs: A Review on Occurrence and Recent Methods of Analysis. Trends in Food Science & Technology, 36, 96-136.
https://doi.org/10.1016/j.tifs.2014.01.005

[9]   Adeyeye, S.A.O. and Yildiz, F. (2016) Fungal Mycotoxins in Foods: A Review. Cogent Food & Agriculture, 2, 1213127.
https://doi.org/10.1080/23311932.2016.1213127

[10]   Kononenko, G., Burkin, A., Gavrilova, O. and Gagkaeva, T. (2015) Fungal Species and Multiple Mycotoxin Contamination of Cultivated Forage Crops. Agricultural and Food Science, 24, 8.

[11]   Piotrowska, M., Slizewska, K. and Biernasiak, J. (2013) Mycotoxins in Cereal and Soybean-Based Food and Feed. In: El-Shemy, P.H., Ed., Soy Bean—Pest Resistance, InTech, Chapter 8.

[12]   Tabuc, C. and Stefan, G. (2005) Assessment of mycologic and mycotoxicologic contamination of Soybean, Sunflower and Rape Seeds and Meals during 2002-2004. Archiva Zootechnica, 8, 51-56.

[13]   Wild, C.P. and Gong, Y.Y. (2010) Mycotoxins and Human Disease: A Largely Ignored Global Health Issue. Carcinogenesis, 31, 71-82.
https://doi.org/10.1093/carcin/bgp264

[14]   IARC (2012) Aflatoxins. In: Chemical Agents and Related Occupations, International Agency for Research on Cancer, Lyon, 225-248.

[15]   IARC (2002) Traditional Herbal Medicines, Some Mycotoxins, Naphthalene and Styrene. In: Monographs on the Evaluation of Carcinogenic Risks to Humans, IARC, Lyon, 82-171.

[16]   IARC (1993) Ochratoxin A. In: Monographs on the Evaluation of Carcinogenic Risks to Humans, International Agency for Research on Cancer, Lyon, 489-521.

[17]   Ugwu, D.S. and Nwoke, U.M. (2011) Assessment of Soybean Products Acceptability and Consumption in Orumba South Local Government Area of Anambra State Nigeria. International Research Journal of Agricultural Science and Soil Science, 1, 314-325.

[18]   Egbuta, M.A., Mwanza, M. and Dutton, M.F. (2015) Evaluation of Five Major Mycotoxins Co-Contaminating Two Cereal Grains from Nigeria. International Journal of Biochemistry Research and Review, 6, 160-169.
https://doi.org/10.9734/IJBCRR/2015/15306

[19]   Pinotti, L., Ottoboni, M., Giromini, C., Dell’Orto, V. and Cheli, F. (2016) Mycotoxin Contamination in the EU Feed Supply Chain: A Focus on Cereal Byproducts. Toxins, 8, 45.
https://doi.org/10.3390/toxins8020045

[20]   Karlovsky, P., Suman, M., Berthiller, F., De Meester, J., Eisenbrand, G., Perrin, I., Oswald, I.P., Speijers, G., Chiodini, A., Recker, T. and Dussort, P. (2016) Impact of Food Processing and Detoxification Treatments on Mycotoxin Contamination. Mycotoxin Research, 32, 179-205.
https://doi.org/10.1007/s12550-016-0257-7

[21]   Krauss, R.M., Deckelbaum, R.J., Ernst, N., Fisher, E., Howard, B.V., Knopp, R.H., Kotchen, T., Lichtenstein, A.H., McGill, H.C., Pearson, T.A., Prewitt, T.E., Stone, N.J., Van Horn, L. and Weinberg, R. (1996) Dietary Guidelines for Healthy American Adults. Circulation, 94, 1795-1800.
https://doi.org/10.1161/01.CIR.94.7.1795

[22]   Egbuta, M.A., Mwanza, M., Njobeh, P.B., Phoku, J.Z., Chilaka, C. and Dutton, M. (2015) Isolation of Filamentous Fungi Species Contaminating Some Nigerian Food Commodities. Journal of Food Research, 4, 38-50.
https://doi.org/10.5539/jfr.v4n1p38

[23]   Phoku, J.Z., Dutton, M.F., Njobeh, P.B., Mwanza, M., Egbuta, M.A. and Chilaka, C.A. (2012) Fusarium Infection of Maize and Maize-Based Products and Exposure of a Rural Population to Fumonisin B1 in Limpopo Province, South Africa. Food Additives and Contaminants: Part A, 29, 1743-1751.
https://doi.org/10.1080/19440049.2012.708671

[24]   Zhou, G., Whong, W.Z., Ong, T. and Chen, B. (2000) Development of a Fungus-Specific PCR Assay for Detecting Low-Level Fungi in an Indoor Environment. Molecular and Cellular Probes, 14, 339-348.
https://doi.org/10.1006/mcpr.2000.0324

[25]   Njobeh, P.B., Dutton, M.F., Koch, S.H., Chuturgoon, A.A., Stoev, S.D. and Mosonik, J.S. (2010) Simultaneous Occurrence of Mycotoxins in Human Food Commodities from Cameroon. Mycotoxin Research, 26, 47-57.
https://doi.org/10.1007/s12550-009-0039-6

[26]   Egbuta, M.A., Chilaka, C.A., Phoku, J.Z., Mwanza, M. and Dutton, M.F. (2013) Co-Contamination of Nigerian Cocoa and Cocoa-Based Powder Beverages Destined for Human Consumption by Mycotoxins. Ethno Medicine, 7, 187-194.

[27]   Shepherd, G.S., Thiel, P.G., Sydenham, E.W., Vleggaar, R. and Alberts, J.F. (1994) Determination of the Mycotoxin Fumonisin B1 and Identification of Its Partially Hydrolysed Metabolites in the Faeces of Nonhuman Primates. Food Chemical Toxicology, 32, 23-29.
https://doi.org/10.1016/0278-6915(84)90032-2

[28]   Makun, H.A., Dutton, M.F., Njobeh, P.B., Mwanza, M. and Kabiru, A.Y. (2011) Natural Multi-Occurrence of Mycotoxins in Rice from Niger State, Nigeria. Mycotoxin Research, 27, 97-104.
https://doi.org/10.1007/s12550-010-0080-5

[29]   Embaby, E.M., Reda, M., Abdel-Wahhab, M.A., Omara, H. and Mokabel, A.M. (2013) Occurrence of Toxigenic Fungi and Mycotoxins in Some Legume Seeds. Journal of Agricultural Technology, 9, 151-164.

[30]   Bhattacharya, K. and Raha, S. (2002) Deteriorative Changes of Maize, Groundnut and Soybean Seeds by Fungi in Storage. Mycopathologia, 155, 135-141.
https://doi.org/10.1023/A:1020475411125

[31]   Milani, J.M. (2013) Ecological Conditions Affecting Mycotoxin Production in Cereals: A Review. Veterinarni Medicina, 58, 405-411.

[32]   Ismaiel, A. and Papenbrock, J. (2015) Mycotoxins: Producing Fungi and Mechanisms of Phytotoxicity. Agriculture, 5, 492-537.
https://doi.org/10.3390/agriculture5030492

[33]   Lawley, R. (2013) Aflatoxins. Food Safety Watch.

[34]   Wang, Y., Wang, L., Liu, F., Wang, Q., Selvaraj, J., Xing, F., Zhao, Y. and Liu, Y. (2016) Ochratoxin A Producing Fungi, Biosynthetic Pathway and Regulatory Mechanisms. Toxins, 8, 83.
https://doi.org/10.3390/toxins8030083

[35]   Abarca, M.L., Bragulat, M.R., Castellá, G. and Cabanes, F.J. (1994) Ochratoxin A Production by Strains of Aspergillus niger var. Niger. Applied and Environmental Microbiology, 60, 2650-2652.

[36]   Mazumder, P.M. and Sasmal, D. (2001) Mycotoxins—Limits and Regulations. Ancient Science of Life, 20, 1-19.

 
 
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