AiM  Vol.6 No.14 , December 2016
Benthic Bacteria Community Changes in Responses to Different Organic Resources from Macrophyte- and Algae-Dominated Areas of Lake Taihu, China
Carbon resources play an important role in affecting the benthic bacterial community in shallow lakes. In this study, pyrosequencing was applied to compare bacteria phylogenic profile in incubated sediments with normal and exchanged organic detritus in macrophyte-dominated East Lake Taihu and algal-dominated Meiliang Bay. We observed significant bacteria species variations in sediments from two bays, regardless of treatments. RDA (Redundancy Analysis) analysis showed that sediment characteristics, especially concentrations of total nitrogen might account for this differentiation. Besides, algal-dominated Meiliang Bay sediment with addition of Vallisneria detritus exhibited higher bacterial species variations than the sediment amended with Microcystis detritus. To the contrary, sediments from macrophyte-dominated East Lake Taihu shared similar bacteria profile at all taxonomic levels and grouped together in MDS (multidimensional scaling) plots over the treatments with Vallisneria or Microcystis detritus addition into the sediment. We speculated that the different degradability of macrophyte detritus and algal detritus led to varied bacterial responses to exchanged organic resources and ultimately, the amounts, nutrient availability and degradability of organic resources may be main reasons for benthic bacteria community structure differentiation between the two states in shallow lakes.
Cite this paper: Tang, Y. , Chen, D. , Yang, X. and Xu, R. (2016) Benthic Bacteria Community Changes in Responses to Different Organic Resources from Macrophyte- and Algae-Dominated Areas of Lake Taihu, China. Advances in Microbiology, 6, 1040-1052. doi: 10.4236/aim.2016.614097.

[1]   Scheffer, M., Hosper, S.H., Meijer, M.L., Moss, B. and Jeppesen, E. (1993) Alternative Equilibria in Shallow Lakes. Trends in Ecology & Evolution, 8, 275-279.

[2]   Scheffer, M., Carpenter, S., Foley, J.A., Folke, C. and Walker, B. (2001) Catastrophic Shifts in Ecosystems. Nature, 413, 591-596.

[3]   von Donk, E. and Gulati, R.D. (1995) Transition of a Lake to Turbid State Six Years after Biomanipulation: Mechanisms and Pathways. Water Science & Technology, 32, 197-206.

[4]   Chen, Y., Qin, B., Teubner, K. and Dokulil, M.T. (2003) Long-Term Dynamics of Phytoplankton Assemblages: Microcystis-Domination in Lake Taihu, a Large Shallow Lake in China. Journal of Plankton Research, 25, 445-453.

[5]   Llames, M.E., Paul, A.G., Zagarese, H., Ferraro, M. and Izaguirre, I. (2013) Alternative States Drive the Patterns in the Bacterioplankton Composition in Shallow Pampean Lakes (Argentina). Environmental Microbiology Reports, 5, 310-321.

[6]   Wu, Q., Zwart, G., Wu, J., Agterveld, M., Liu, S. and Hahn, M.W. (2007) Submersed Macrophytes Play a Key Role in Structuring Bacterioplankton Community Composition in the Large, Shallow, Subtropical Taihu Lake, China. Environmental Microbiology, 9, 2765-2774.

[7]   Fallon, R.D. and Brock, T.D. (1980) Planktonic Blue-Green Algae: Production, Sedimentation, and Decomposition in Lake Mendota, Wisconsin. Limnology and Oceanography, 25, 72-88.

[8]   Takamura, N. and Yasuno, M. (1988) Sedimentation of Phytoplankton Populations Dominated by Microcystis in a Shallow Lake. Journal of Plankton Research, 10, 283-299.

[9]   Mann, K.H. (1988) Production and Use of Detritus in Various Freshwater, Estuarine, and Coastal Marine Ecosystems. Limnology and Oceanography, 33, 910-930.

[10]   Howarth, R.W. (1993) Microbial Processes in Salt-Marsh Sediments. In: Ford, T.E., Ed., Aquatic Microbiology: An Ecological Application. Blackwell, Massachusetts, 239-259.

[11]   Shao, K., Gao, G., Qiang, B., et al. (2011) Comparing Sediment Bacterial Communities in the Macrophyte-Dominated and Algae-Dominated Areas of Eutrophic Lake Taihu, China. Canadian Journal of Microbiology, 57, 263-272.

[12]   Shao, K., Gao, G., Wang, Y., Tang, X. and Qin, B. (2013) Vertical Diversity of Sediment Bacterial Communities in Two Different Trophic States of the Eutrophic Lake Taihu, China. Journal of Environmental Sciences, 25, 1186-1194.

[13]   Ronaghi, M. (2001) Pyrosequencing Sheds Light on DNA Sequencing. Genome Research, 11, 3-11.

[14]   Hamady, M., Walker, J.J., Harris, J.K., Gold, N.J. and Knight, R. (2008) Error-Correcting Barcoded Primers for Pyrosequencing Hundreds of Samples in Multiplex. Nature Methods, 5, 235-237.

[15]   Jones, S.E. and Lennon, J.T. (2010) Dormancy Contributes to the Maintenance of Microbial Diversity. Proceedings of the National Academy of Sciences, 107, 5881-5886.

[16]   Lennon, J.T. and Jones, S.E. (2013) Microbial Seed Banks: The Ecological and Evolutionary Implications of Dormancy. Nature Reviews, 9, 119-130.

[17]   Qin, B.Q., Xu, P., Wu, Q., Luo, L. and Zhang, Y. (2007) Environmental Issues of Lake Taihu, China. Hydrobiologia, 581, 3-14.

[18]   Black, K.D., Calder, L.A., Nickell, T.D., et al. (2012) Chlorophyll, Lipid Profiles and Bioturbation in Sediments around a Fish Cage Farm in the Gulf of Eilat, Israel. Aquaculture, 356-357, 317-327.

[19]   Ritchie, R.J. (2006) Consistent Sets of Spectrophotometric Chlorophyll Equations for Acetone, Methanol and Ethanol Solvents. Photosynthesis Research, 89, 27-41.

[20]   Adersson, A.F., Lindberg, M., Jakobsson, H.,Bäckhed, F., Nyrén, P. and Engstrand, L. (2008) Comparative Analysis of Human Gut Microbiota by Barcoded Pyrosequencing. PLoS ONE, 3, e2836.

[21]   Quince, C., Lanzen, A., Davenport, R.J. and Turnbaugh, P.J. (2011) Removing Noise from Pyrosequenced Amplicons. BMC Bioinformatics, 12, 38.

[22]   Edgar, R., Haas, B.J., Clemente, J.C., Quince, C. and Knight, R. (2011) UCHIME Improves Sensitivity and Speed of Chimera Detection. Bioinformatics, 27, 2194-2200.

[23]   Schloss, P.D., Westcott, S.L., Ryabin, T., et al. (2009) Introducing Mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities. Applied and Environmental Microbiology, 75, 7537-7541.

[24]   Cole, J., Wang, Q., Cardenas, E., et al. (2009) The Ribosomal Database Project: Improved Alignments and New Tools for rRNA Analysis. Nucleic Acids Research, 37, D141-D145.

[25]   Caporaso, J.G., Kuczynski, J., Stombaugh, J., et al. (2010) QIIME Allows Analysis of High-Throughput Community Sequencing Data. Nature Methods, 7, 335-336.

[26]   Bray, J.R. and Curtis, J.T. (1957) An Ordination of the Upland Forest Communities of Southern Wisconsin. Ecological Monographs, 27, 325-349.

[27]   Spring, S., Schulze, R., Overmann, J. and Schleifer, K.-H. (2000) Identification and Characterization of Ecologically Significant Prokaryotes in the Sediment of Freshwater Lakes: Molecular and Cultivation Studies. FEMS Microbiology Reviews, 24, 573-590.

[28]   Bai, Y.H., Shi, Q., Wen, D.H., et al. (2012) Bacterial Communities in the Sediments of Dianchi Lake a Partitioned Eutrophic Waterbody in China. PLoS ONE, 7, e37796.

[29]   Yu, J., Li, Y., Liu, X., et al. (2013) The Fate of Cyanobacterial Detritus in Food Web of Lake Taihu: A Mesocosm Study Using 13C and 15N Labeling. Hydrobiologia, 710, 39-46.

[30]   Duarte, C.M. (1992) Nutrient Concentration of Aquatic Plants: Patterns Cross Species. Limnology and Oceanography, 37, 882-889.

[31]   Ye, L., Wu, X., Tan, X., et al. (2010) Cell Lysis of Cyanobacteria and Its Implications for Nutrient Dynamics. International Review of Hydrobiology, 95, 235-245.

[32]   Wicks, R.J., Moran, M.A., Pittman, L.J. and Hodson, R.E. (1991) Carbohydrate Signatures of Aquatic Macrophytes and Their Dissolved Degradation Products as Determined by a Sensitive High-Performance Ion Chromatography Method. Applied and Environmental Microbiology, 57, 3135-3143.

[33]   Cunha, A., Almeida, A., Coelho, F.J.R.C., Gomes, N.C.M., Oliveira, V. and Santos, A.L. (2010) Bacterial Extracellular Enzymatic Activity in Globally Changing Aquatic Ecosystems. In: Mendez-Vilas, A., Ed., Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, Formatex Research Center, Badajoz, 124-135.

[34]   Middelburg, J.J., Nieuwenhuize, J., Lubberts, R.K. and Plassche, O.V.D. (1997) Organic Carbon Isotope Systematics of Coastal Marshes. Estuarine, Coastal and Shelf Science, 45, 681-687.

[35]   Boschker, H.T.S., de Brouwer, J.F.C. and Cappenberg, T.E. (1999) The Contribution of Macrophyte-Derived Organic Matter to Microbial Biomass in Salt-Marsh Sediments: Stable Carbon Isotope Analysis of Microbial Biomarkers. Limnology and Oceanography, 44, 309-319.

[36]   Bouillon, S. and Boschker, H.T.S. (2006) Bacterial Carbon Sources in Coastal Sediments: A Cross-System Analysis Based on Stable Isotope Data of Biomarkers. Biogeosciences, 3, 175-185.