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 JBM  Vol.4 No.12 , December 2016
Point-of-Care Testing Using Three Dimensional Optical Biosensor Based on Microfluidic Technology
Abstract:
We have presented a three dimensional optical protein chip that fulfills the demanding for point-of-care diagnostics in terms of ease-of-use (one step assay), miniaturization (5 μl). The artful combination of magnetic nanoparticles on chip and total internal reflection imaging (TIRI) technology permits the sensitive and rapid detection of hs-CRP (high-sensitivity C-reactive protein). The whole test was complete within 10 min using “all in one step” assay with a limit of detection of 0.1 ng/mL hs-CRP. The measuring range for hs-CRP could be extended to 10 ng/mL. The chip can also be used to detect more parameters in blood samples.
Cite this paper: Liu, C. , Cai, H. , Jia, J. , Cao, T. , Li, T. , Ma, T. and Liu, C. (2016) Point-of-Care Testing Using Three Dimensional Optical Biosensor Based on Microfluidic Technology. Journal of Biosciences and Medicines, 4, 56-61. doi: 10.4236/jbm.2016.412009.
References

[1]   Natalia, L.B., Jorge, D.G. and Johann, F.O. (2016) The Future Point-of-Care Detection of Disease and Its Data Capture and Handling. Anal Bioanal Chem, 408, 2827-2837. http://dx.doi.org/10.1007/s00216-015-9249-2

[2]   Jung, W., Han, J., Choi, J.W. and Ahn, C.H. (2015) Point-of-Care Testing (POCT) Di-agnostic Systems Using Microfluidic Lab-on-a-Chip Technologies. Microelectronic Engineering, 132, 46-57. http://dx.doi.org/10.1016/j.mee.2014.09.024

[3]   Ouellette, A.L., Li, J.J., Cooper, D.E., Ricco, A. and Kovacs, G.T.A. (2009) Evolving Point- of-Care Diagnostics Using Up-Converting Phosphor Bioanalytical Systems. Analytical Chemistry, 81, 3216-3221. http://dx.doi.org/10.1021/ac900475u

[4]   Luppa, P.B., Müller, C., Schlichtiger, A. and Schlebusch, H. (2011) Point-of-Care Testing (POCT): Current Techniques and Future Perspectives. Trac Trends in Analytical Chemistry, 30, 887-898. http://dx.doi.org/10.1016/j.trac.2011.01.019

[5]   Yang, Z. and Zhou, D.M. (2006) Cardiac Markers and Their Point-of-Care Testing for Diag-nosis of Acute Myocardial Infarction. Clinical Biochemistry, 39, 771-780. http://dx.doi.org/10.1016/j.clinbiochem.2006.05.011

[6]   Pfäfflin, A. and Schleicher, E. (2009) Inflammation Markers in Point-of-Care Testing (POCT). Analytical & Bioanalytical Chemistry, 393, 1473-1480. http://dx.doi.org/10.1007/s00216-008-2561-3

[7]   Kiechle, L.F. (2015) Three POCT Molecular Applications: A View into Future Devel-opment, Point of Care. The Journal of Near-Patient Testing & Technology, 14, 95-98. http://dx.doi.org/10.1097/poc.0000000000000052

[8]   Baldini, F., Bolzoni, L., Giannetti, A., et al. (2009) A New Procalcitonin Optical Immunosensor for POCT Applications. Analytical and Bioanalytical Chemistry, 393, 1183-1190. http://dx.doi.org/10.1007/s00216-008-2547-1

[9]   Rascher, D., Geerlof, A., Kremmer, E., Krämer, P. and Schmid, M. (2014) Total Internal Reflection (TIRF)-Based Quantification of Procalcitonin for Sepsis Diagnosis—A Point- of-Care Testing Application. Biosensors & Bio-electronics, 59C, 251-258. http://dx.doi.org/10.1016/j.bios.2014.03.052

[10]   Mejri, M.B., Baccar, H., Ktari, T., Aouni, M. and Abdelghani, A. (2011) Detection of E. coli Bacteria Using Impedance Spectroscopy and Surface Plasmon Resonance Imaging Based Biosensor. Sensor Letters, 9, 1-3. http://dx.doi.org/10.1166/sl.2011.1747

[11]   Michael, J.O., Victor, H.P., Brueck, S.R.J. and Gabriel, P.L. (2001) A Surface Plasmon Reson-ance Array Biosensor Based on Spectroscopic Imaging. Biosensors & Bioelectronics, 16, 97-108. http://dx.doi.org/10.1016/S0956-5663(00)00137-8

[12]   Tan, H.M., Pechprasarn, S., Zhang, J., Pitter, M.C. and Somekh, M.G. (2016) High Resolution Quantitative Angle-Scanning Widefield Surface Plasmon Microscopy. Scientific Reports, 6, Article Number: 20195. http://dx.doi.org/10.1038/srep20195

 
 
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