JBM  Vol.4 No.12 , December 2016
Construction of Overexpression Vector of ACBP Gene in Zygosaccharomyces rouxii
The Zygosaccharomyces rouxii is a kind of fermentation yeast which yield flavoring substance in the production of soy sauce. In order to the overexpression of the target protein in wild type strains, we choose PYEs2.0 as the original carrier, the acyl-coA binding protein (ACBP) and GFP gene have been cloned in the multiple cloning site. The screening of labeled URA3 gene was replaced by KanMX gene which anti G418. The vector was obtained through the screening of G418 at the concentration of 25 ug/ml.
Cite this paper: Chen, F. , Han, J. , Liu, L. , Hu, J. , Zhang, X. and Zeng, B. (2016) Construction of Overexpression Vector of ACBP Gene in Zygosaccharomyces rouxii. Journal of Biosciences and Medicines, 4, 23-30. doi: 10.4236/jbm.2016.412004.

[1]   Golden, D.A., Beuchat, L.R. and Hitchcock, H.L. (1994) Changes in Fatty Acid Composition of Various Lipid Components of Zygosaccha-romyces rouxii as Influenced by Solutes, Potassium Sorbate and Incubation Temperature. International Journal of Food Microbiology, 21, 293-303.

[2]   Færgeman, N.J., Sigurskjold, B.W., Kragelund, B.B., Andersen, K.V. and Knudsen, J. (1996) Thermodynamics of Ligand Binding to Acylcoenzyme A Binding Protein Studied by Titration Calorimetry. Biochemistry, 35, 14118-14126.

[3]   Rasmussen, J.T., Faergeman, N.J., Kristiansen, K. and Knudsen, J. (1994) Acyl-CoA Binding Protein (ACBP) Can Mediate Intermembrane Acyl-CoA Transport and Donate Acyl-CoA for Beta-Oxidation and Glyce-rolipid Synthesis. Biochem J., 299, 165-170.

[4]   Rasmussen, J.T., Rosendal, J. and Knudsen, J. (1993) Interaction of Acyl-CoA-Binding Protein (ACBP) on Processes for Which Acyl-CoA Is a Substrate, Product or Inhibitor. Biochem J., 292, 907-913.

[5]   Cohen Simonsen, A., Bernchou Jensen, U., Faergeman, N.J., Knudsen, J. and Mouritsen, O.G. (2003) Acyl-Coenzyme A Organizes Laterally Inmembranes and Is Recognized Specifically by Acyl-Coenzyme A Binding Protein. FEBS Lett., 552, 253-258.

[6]   Wach, A., Brachat, A. and Pohlmann, R. (1994) New Heterologous Modules for Clas-sical or PCR-Based Gene Disruptions in Saccharomyces Cerevisiae. Yeast, 10, 1793-1808.

[7]   Kragelund, B.B., Knudsen, J. and Poulsen, F.M. (1999) Acyl-Coenzyme A Binding Protein (ACBP). Biochimica et Biophysica Acta, 1441, 150-161.

[8]   Kataoka, S. (2005) Functional Effects of Japanese Style Fermented Soy Sauce (Shoyu) and Its Components. J Biosci Bioeng., 100, 227-234.

[9]   Hou, L.H., Song, Q. and Cao, X.H. (2009) Research Progress of Soy-Sauce Flavour. China Brewing, 7, 1-3.