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 JBM  Vol.4 No.11 , November 2016
Synthesis and Characterization of Crosslinked Hydrogel of Konkoli (Maesopsis eminii) Grafted Polymethylacrylamide for a Preliminary Study as an Insulin Delivery System
Abstract: Polysaccharide has lately received a significant attention in the formulation of drug delivery system based on the abundant availability, non-toxicity and the various ways its nature, structure and functionality can be modified. In this preliminary work on Konkoli (Maesopsis eminii) galactomannan (KG), it was modified by grafting with methacrylamide (MAAm) using ammonium persulphate (ASP) as initiator. The grafted galactomannan was then crosslinked using N, N-methylenebisacrylamide (N, N-MBAAm) to produce the hydrogel called konkoli grafted polymethylacrylamide (KG-g-poly (MAAm)). FTIR analyses confirm crosslinking and other changes in the functionality of KG-g-poly (MAAm) compared to KG. Swelling properties which are fundamental to the potential properties of any hydrogel as a drug delivery system were studied for KG-g-poly (MAAm) with varied amount of monomer (MAAm), crosslinker and pH with respect to time and temperature. There was a rapid rise followed by a dramatic fall in the swelling capacity with increase in both monomer and crosslinker concentration. The swelling capacity of KG-g-poly (MAAm) also improves as the pH of the medium was changed from acidic to alkaline. Generally, the swelling capacity of KG-g-poly (MAAm) increases with time and temperature of immersion. This result therefore encourages further studies as it presents KG-g-poly (MAAm) potentials in such application as insulin delivery system.
Cite this paper: Ugwu, O. , Barminas, J. , Nkafamiya, I. and Akinterinwa, A. (2016) Synthesis and Characterization of Crosslinked Hydrogel of Konkoli (Maesopsis eminii) Grafted Polymethylacrylamide for a Preliminary Study as an Insulin Delivery System. Journal of Biosciences and Medicines, 4, 36-47. doi: 10.4236/jbm.2016.411005.
References

[1]   Deshmukh, C.D. and Jain, A. (2015) Diabetes Mellitus: A Review. International Journal of Pure & Applied Bioscience, 3, 224-230.

[2]   Rother, K.I. (2007) Diabetes Treatment—Bridging the Divide. New England Journal of Medicine, 356, 1499-1501.
https://doi.org/10.1056/NEJMp078030

[3]   Tierney, L.M, McPhee, S.J. and Papadakis, M.A. (2002) Current Medical Diagnosis & Treatment. International Edition. Lange Medical Books/McGraw-Hill, New York, 1203-1215.

[4]   Ganguly, K., Chaturvedi, K., More, U.A., Nadagouda, M.N. and Minabhavi, T.M. (2014) Polysaccharide-Based Micro/Nanohydrogels for Delivering Macromolecular Therapeutics. Journal of Controlled Release, 193, 162-173.
https://doi.org/10.1016/j.jconrel.2014.05.014

[5]   Owens, D.R. (2002) New Horizons-Alternative Routes for Insulin Therapy. Nature Review: Drug Discovery, 1, 529-540.
https://doi.org/10.1038/nrd836

[6]   Cefalu, W.T. (2004) Concept, Strategies and Feasibility of Non-Invasive Insulin Delivery. Diabetes Care, 27, 239-246.
https://doi.org/10.2337/diacare.27.1.239

[7]   Singh, B., Chauhan, G.S., Kumar, S. and Chauhan, N. (2007) Synthesis, Characterization and Swelling Responses of pH Sensitive Psyllium and Polyacrylamide Basedhydrogels for the Use in Drug Delivery (I). Carbohydrate Polymer, 67, 190-200.
https://doi.org/10.1016/j.carbpol.2006.05.006

[8]   Son, K.H. and Lee, J.W. (2016) Synthesis and Characterization of Poly(Ethylene Glycol) Based Thermo-Responsive Hydrogels for Cell Sheet Engineering. Materials, 9, 854.
https://doi.org/10.3390/ma9100854

[9]   Zhao, W., Jin, X., Cong, X., Liu, Y. and Fu, J. (2013) Degradable Natural Polymer Hydrogels for Articular Cartilage Tissue Engineering. Journal of Chemical Technology & Biotechnology, 88, 327-339.
https://doi.org/10.1002/jctb.3970

[10]   Gökçeören, A.T., Senkal, B.F. and Erbil, C. (2014) Effect of Crosslinker Structure and Crosslinker/Monomer Ratio on Network Parameters and Thermodynamic Properties of Poly (N-isopropylacrylamide) Hydrogels. Journal of Polymer Research, 21, 1-12.
https://doi.org/10.1007/s10965-014-0370-2

[11]   Brannon-Peppas, L. (1997) Biomaterials: Polymers in Controlled Drug Delivery, Medical Plastics and Biomaterials Magazine. www.m.mddionline.com

[12]   Giammanco, G.E., Carrion, B., Coleman, R.M. and Ostrowski, A.D. (2016) Photoresponsive Polysaccharide-Based Hydrogels with Tunable Mechanical Properties for Cartilage Tissue Engineering. ACS Applied Materials & Interfaces, 8, 14423-14429.
https://doi.org/10.1021/acsami.6b03834

[13]   Mao S., Sun, W. and Kissel, T. (2010) Chitosan-Based Formulations for Delivery of DNA and siRNA. Advanced Drug Delivery Reviews, 62, 12-27.
https://doi.org/10.1016/j.addr.2009.08.004

[14]   Kost, J., Horbett, T.A. and Ratner, B.D. (1985) Glucose-Sensitive Membranes Containing Glucose Oxidase: Activity, Swelling, and Permeability Studies. Journal of Biomedical Material Research, 19, 1117-1133.
https://doi.org/10.1002/jbm.820190920

[15]   Ishihara, K., Kobayashi, M., and Shinohara, I. (1983) Control of Insulin Permeation through a Polymer Membrane with Responsive Function for Glucose. Makromolecules and Chemical Rapid Communication, 4, 327-338.
https://doi.org/10.1002/marc.1983.030040511

[16]   Heller, J., Pangburn, S.H. and Penhale, D.W.H. (1987) Use of Bioerodible Polymers in Self-Regulated Drug Delivery Systems, In: Lee, P.I. and Good, W.R., Eds., Controlled-Release Technology, Pharmaceutical Applications, Washington DC, ACS Symposium Series, 172-187.
https://doi.org/10.1021/bk-1987-0348.ch013

[17]   Kim, S.W. (1996) Temperature Sensitive Polymers for Delivery of Macromolecular Drugs, In: Ogata, N., Kim, S.W. and Feijen, J., Eds., Advanced Biomaterials in Biomedical Engineering and Drug Delivery Systems, Springer, Tokyo, 126-133.
https://doi.org/10.1007/978-4-431-65883-2_25

[18]   Dorski, C.M., Doyle, F.J. and Peppas, N.A. (1997) Preparation and Characterization of Glucose-Sensitive P(MAA-g-EG) Hydrogels. Polymer Materials Science Engineering Proceedings, 76, 281-282.

[19]   Pan, Y., Li, Y., Zhao, H., Zheng, J., Xu, H., Wei, G., Hao, J. and Cui, F. (2002) Bioadhesive Polysaccharide in Protein Delivery System: Chitosan Nanoparticles Improve the Intestinal Absorption of Insulin in Vivo. International Journal Pharmaceutics, 249, 139-147.
https://doi.org/10.1016/S0378-5173(02)00486-6

[20]   Barminas, J.T. (2004) Some Studies on Solution Behaviour of Konkoli (Maesopsis eminii) Seed Gum. Ph.D. Thesis, Federal University of Technology Yola, Nigeria.

[21]   Osemeahon, S.A., Barminas, J.T., Aliyu, B.A. and Nkafamiya, I.I. (2008) Development of Sodium Alginate and Konkoli Gum-Graft-Polyacrylamide Blend Membrane Optimization of Grafting Conditions. African Journal of Biotechnology, 7, 1309-1313.

[22]   Singh, B. and Sharma, N. (2009) Modification of Psyllium Polysaccharides for Use in Oral Insulin Delivery. Food Hydrocolloids, 23, 928-935.
https://doi.org/10.1016/j.foodhyd.2008.06.004

[23]   Pourjavadi, A. and Mahdavinia, G.R. (2006) Superabsorbency, pH-Sensitivity and Swelling Kinetics of Partially Hydrolyzed Chitosan-g-poly(Acrylamide) Hydrogels. Turkish Journal of Chemistry, 30, 595-608.

[24]   Clara, I., Lavanya, R. and Natchimuthu, N. (2016) pH and Temperature Responsive Hydrogels of Poly(2-acrylamido-2-methyl-1-propanesulfonic acid-co-methacrylic acid): Synthesis and Swelling Characteristics. Journal of Macromolecular Science, Part A; Pure and Applied Chemistry, 53, 492-499.
https://doi.org/10.1080/10601325.2016.1189282

[25]   Kim, S.J., Lee, K.J. and Kim, S.I. (2004) Swelling Behavior of Polyelectrolyte Complex Hydrogels Composed of Chitosan and Hyaluronic Acid. Journal of Applied Polymer Science, 93, 1097-1101.
https://doi.org/10.1002/app.20560

 
 
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