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 AiM  Vol.6 No.13 , November 2016
Detection of Antibodies against Mycoplasma mycoides subsp. capri in Goats with the Complement Fixation Test
Abstract:
Mycoplasma mycoides subsp. capri is the causative agent of severe and acute respiratory problems in goats, which spreads rapidly and represents high mortality. The serological profile of the goat population, from nine regions in seven states of Mexico, was screened by the Complement Fixation test (CF) in sera from asymptomatic goats and animals with mild respiratory symptoms. Sera and nasal swabs of 827 goats were collected for the isolation of the organism. An antiserum was prepared against a previously isolated field strain of Mycoplasma mycoides subsp. capri. CF Antibody titers were associated with the results of the isolates to determine the cutoff point. The CF was considered as positive if its result was ≥1/16. The CF registered 251 positive goats (30.35%) and 576 (69.65%) negative; the test showed high sensitivity (93.33%) and specificity (72.27%). In the specific case of diagnosis for mycoplasmosis associated with respiratory problems in goats, the CF proved to be a good diagnosis test, this study determined that 30% of the goat population showed antibody titers against Mycoplasma mycoides subsp. capri and revealed those animals who have had contact with this microorganism during their lives regardless of the presence or absence of respiratory symptoms.
Cite this paper: Corona-Vargas, J. , Vicencio-Mallén, M. , Salmerón-Sosa, F. , Carrillo-Casas, E. , Trigo-Tavera, F. and Miranda-Morales, R. (2016) Detection of Antibodies against Mycoplasma mycoides subsp. capri in Goats with the Complement Fixation Test. Advances in Microbiology, 6, 959-964. doi: 10.4236/aim.2016.613090.
References

[1]   Cottew, G.S., Breard, A., DaMassa, A.J., Erno, H., Leach, R.H., LeFevre, P.C., Rodwell, A.W. and Smith, G.R. (1987) Taxomomy of the Mycoplasma mycoides Cluster. Israel Journal of Medical Science, 23, 632-635.

[2]   BÖlske, G., Mattsson, J.G., Bascuñana, C.R., Bergstrom, K., Wesonga, H. and Johansson, K.E. (1996) Diagnosis of Contagious Caprine Pleuropneumonia by Detection and Identification of Mycoplasma capricolum subsp. capripneumoniae by PCR and Restriction Enzyme Analysis. Journal of Clinical Microbiology, 34, 785-791.

[3]   Rurangirwa, F.R. and McGuire, T.C. (2004) Contagious Caprine Pleuropneumonia: Diagnosis and Control. Department of Veterinary Microbiology and Pathology Washington State University.

http://www.fao.org/wairdocs/ilri/x5473b/x5473b11.htm

[4]   Bergonier, D., Berthelot, X. and Poumarat, F. (1997) Contagious Agalactia of Small Ruminants: Current Knowledge Concerning Epidemiology, Diagnosis and Control. Revue Scientifique et Technique Office International of Epizootics, 16, 848-873.

[5]   Nicholas, R.A.J. (2002) Improvements in the Diagnosis and Control of Diseases of Small Ruminants Caused by Mycoplasmas. Small Ruminant Research, 45, 145-149.

http://dx.doi.org/10.1016/S0921-4488(02)00095-0

[6]   Jaramillo, M.L., Cruz, S.T., Pijoan, A. C. and Ciprian, C.A. (1983) Caracterización de micoplasmas aislados de cabras en México. Memorias Reunión Anual de Investigación Pecuaria en México. SARH-UNAM, México City, 414-417.

[7]   Hernández, L., López, J., St-Jacques, M., Ontiveros, L., Acosta, J. and Andel, K. (2006) Mycoplasma mycoides subsp. capri Associated with Goat Respiratory Disease and High Flock Mortality. Canadian Veterinary Journal, 47, 366-369.

[8]   Tully, J.G. and Clyde, S. (1983) Mycoplasma Techniques Course: Preparation of Mycoplasma antisera. International Organization for Mycoplasmology (IOM), Bordeaux.

[9]   Cunningham, C.H. (1986) Virología práctica. 6th Edition, España, Acribia.

[10]   Paling, R.W., Macowan, K.J. and Karstad, L. (1978) The Prevalence of Antibody to Contagious Caprine Pleuropneumonia (Mycoplasma Strain F38) in Some Wild Herbivores and Camels in Kenya. Journal of Wildlife Diseases, 14, 305-308.

http://dx.doi.org/10.7589/0090-3558-14.3.305

[11]   Muthomi, E.K. and Rurangirwa, F.R. (1983) Passive Haemagglutination and Complement Fixation as Diagnostic Tests for Contagious Caprine Pleuropneumonia Caused by the F-38 Strain of Mycoplasma. Research in Veterinary Science, 35, 1-4.

[12]   Jones, G.E. and Wood, A.R. (1988) Microbiological and Serological Studies on Caprine Pneumonias in Oman. Research in Veterinary Science, 44, 125-131.

[13]   March, J.B., Gammack, C. and Nicholas, R. (2000) Rapid Detection of Contagious Caprine Pleuropneumonia Using a Mycoplasma capricolum subsp capripneumoniae Capsular Polysaccharide-Specific Antigen Detection Latex Agglutination Test. Journal of Clinical Microbiology, 38, 4152-4159.

[14]   Cho, H.J., Ruhnke, H.L. and Langford, E.V. (1975) The Indirect Hemagglutination Test for the Detection of Antibodies in Cattle Naturally Infected with Mycoplasmas. Canadian Journal of Comparative Medicine, 40, 20-29.

[15]   Solana, M.P. and Udave, L.M. (1965) Estudios epizootiológicos de la pleuroneumonía contagiosa de las cabras. Revista Mexicana De Ciencias Pecuarias, 0(6), 16-24.

[16]   Solana, P. and Rivera, E. (1967) Infection of Goats in Mexico by Mycoplasma mycoides var capri. Annals of the New York Academy of Sciences, 143, 357-363.

http://dx.doi.org/10.1111/j.1749-6632.1967.tb27676.x

 
 
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