Mastication, the first step of digestion, is defined as a process by which the food placed in the mouth is mechanically modified to make it suitable for swallowing  . The teeth are essential for grinding food and to prepare the food bolus for digestion, the tooth loss leads to impair mastication function  and sufficient antagonistic tooth contact is required to chew food  .
In early days, there was a tendency to replace all missing teeth  ; however, it’s generally accepted that it is not required to replace all teeth to restore oral function. The shortened denture arch concept is well documented since its introduction by Kayser A.F. et al., Gotfredsen K. et al., Kanno T. et al., twelve anterior teeth and eight posterior teeth are necessary and sufficient to ensure the mandibular stability required for a good mastication provided that the teeth are effective    . A study of Gerritsen, Witter et al. indicates that a shortened dental arch is a sustainable solution for partially edentulous patients, as they report that 87% of the patients with shortness dental arcade (SDA) were still in the same condition after more time than 40 years of function  .
All oral diseases, which affect the number, structure and position of the teeth are supposed to have an impact on the quality of life, on the mastication and consequently on the nutrition   . Replacing lost teeth by means of a removable denture is expected to improve mastication and thereby nutrition. The other authors indeed found an improved ability to reduce the bolus particle size when using partial removable denture, although the masticatory function and the teeth’s sensory function  could not be re-established completely. Also applies to other studies, however, indicate that oral rehabilitation by means of partial denture does not lead to improved energy and nutrient intake   . These results attenuate the association of mastication with food and/or nutritional intake and indicate that mastication alone cannot explain food and/or nutrient intake. If food choice was exclusively influenced by impaired mastication, the provision of dentures to partially edentulous persons would improve their diet. However, intervention studies have not shown any significant effects even after the provision of new prosthesis. It is assumed that oral health, particularly masticatory function, may contribute to the food selection of elderly people.
One of the main objectives of the oral rehabilitation with acrylic partial removable denture is to improve the masticatory function by replacing the missing teeth  . Besides the uncertainty about its effect on oral function, partial denture treatment also holds risks for the remaining dentition. The recent study of Jepson N.J et al. showed that, removable partial dentures (RPDs) have the potential to negatively impact different aspects of oral health  . Furthermore, there are very few studies  that have investigated whether the RPDs have any impact on general health. From the limited literature available, it appears that RPDs can possibly improve quality of life, and this is relevant in the era of patient-centered care.
Studies have examined the effects of the fixed prosthesis and implants on masticatory function  , but the physiological impact of the rehabilitation by partial removable prosthesis has poorly been studied and the outcomes are not straightforward  . And no study found has been assessed the objective OHIP except the preliminary study of Sekele that has been assessed the subjective OHIP in two-year function of RDP  . The aim of this study was therefore to evaluate the effect of partial removable denture treatment on oral health related quality of life and masticatory function after 5 years of function.
2. Materials and Methods
It’s an interventional prospective study of all partially edentulous patients (except patients with class 4 of Kennedy) presenting for removable denture treatment at the Prosthodontics Service in the Hospital Affiliated to Kinshasa University, Democratic Republic of Congo between May 2008 to August 2008, were assessed in this study. Criteria for exclusion were joint diseases (n = 22), hematological diseases (n = 3), allergies (n = 19), current or history of chemotherapy and radiotherapy (n = 1), on a special diet prescribed by a physician (n = 17), a DPSI equal to or above 2 after oral hygiene instructions (n = 20), carious or infection of endodontic teeth (n = 25), and unwilling to participate in the study due to refusal to be without prosthesis when selected to be in the non-denture group. After this applying exclusion criteria and after receiving oral hygiene instructions and providing informed consent, 379 patients (between 19 to 89 years old) were included in the study. They were randomly assigned into 2 groups. One group of patients received a removable acrylic denture (denture group, n = 189) than the other group did not (non-denture group, n = 189). After five years of function, 138 patients were still in the study (60 for the denture group and 78 for the non-denture group). 241 participants lost to follow-up: dropped out because they moved to another town (n = 24), because they did not respond to the recall invitation (n = 181), because of a changed oral status (n = 24), and due to death (n = 12). It was after informed consent of patients and obtained an ethical committee from the School of health of medicine faculty of Kinshasa University number ESP/CE/018 bis/2009 du 09 Jun 2009 that the study started  .
2.1. Oral Health Related Quality of Life
Oral health related quality of life was evaluated using the OHIP-23   and adapted on the African context. Each question had to be answered by yes or no, depending on whether the participants was satisfied (outcome = 1) or dissatisfied (outcome = 0) about a specific aspect of his or her oral health related quality of life. The overall oral health related quality of life per participant was represented by the sum of the outcomes of the individual questions. The questions are similar for both groups, although the formulation of the questions was according to the participants’ oral condition (wearing a denture or not).
2.2. Masticatory Function
Mastication kinetics was evaluated by measuring the mastication time (MaT), number of chewing cycles (MaC), and mastication frequency (MaF = MaC/MaT) required to chew 5 grams of roasted peanuts until it was considered appropriate for swallowing. The test was performed twice per participant and Video recording was used for the evaluation of kinetic parameters of the mastication, also the time of each sequence of chewing. A stopwatch and the number of cycles was counted by two independent investigators. Instead of swallowing, the participants were asked to spit the chewed peanuts in a cup and the participants were asked to rinse with water several times to clear the mouth. Also the rinsing water was collected in the cup. The sizes of the peanut fragments (FraS) were measured in mm and used to evaluate mastication efficacy. Particle size analyses were performed at the central laboratory of nuclear research of Kinshasa University. The crushed food or homogenates was separated from the supernatant and was left in the open air for dissecation in 48 hours before the sifting. The homogenates were passed through a decreasing sieve mesh column 5.6 mm, 4 mm, 2 mm, 1 mm, 0.5 mm, 0.25 mm and 0.125 mm (Retsh AS 200, Retsh GmbH, Haan, Germany) (Figure 1). The homogenates were sifted in the laboratory under vibrator that was adjusted so standardized to its maximum power for a period of one minute.
After each sifting, the content of each sieve was weighed with an analytical balance (Sartorius model CPA225D, Sartorius GmbH, Goettingen, Germany), with a sensitivity of 0.00001 g.
The outcome variables of OHIP, MaT, MaC, MaF, and FraS in the denture and non- denture group were compared using ANOVA (SPSS 20.0, Inc, and Chicago, IL, U.S.A). Statistical significance was set at p < 0.05.
The average age in this study in denture group and no-denture-group was 53.15 ± 22.05 and 31.59 ± 11.98 years. Women have been more participated than the men in both groups with respectively 36 (60%) and 50 (64%) (Table 1). In the denture and the non-denture group, 24 and 21 patients had a Kennedy Class 1 edentulism, 12 and 18 patients had a Kennedy Class 2 edentulism, and 24 and 39 patients had a Kennedy Class III edentulism respectively. The result from OHIP-23 variable showed that the participants enrolled in the non-denture-group were most satisfied on the oral health related quality of life than the denture-group by affected appearance, respiration, the bad taste, taking food, having pain in jaw, eating pain, dental pain and anxiety. However, there are some non-significant differences between these two groups for the appearance (p =
Figure 1. Sieves image.
Table 1. Masticatory parameters according to gender in both groups.
Legend: MaC = number of mastication cycles, MaT = mastication time, MaF = mastication frequency, and FraS = peanut fragment size in mm.
0.3), bad taste (p = 0.3), pain during the dinner (p = 0.4), anxiety or unrest (p = 0.1), physical disability with diminished taste (p = 0.3), dental brushing (p = 0.3), avoid to eat (p = 0.2), diet (p = 0.3), not easy at repast (p = 0.3) and p = 0.2 for avoid to laugh (Table 2).
The masticatory cycle of denture-group was higher compared by non-denture-group, the same to gender variable with 137 ± 47.47 and 107 ± 56.17, against 80.85 ± 20.11 and 76.30 ± 26.38 for the non-denture-group respectively. But in the non-denture group, the mastication time for women was longer than men (Table 1).
Table 3 showed that, the distribution of MaC, MaT, MaF, and FraS were increased in denture than non-denture. The increasing distribution of MaC in both group was depending to the number of occluding pairs, but FraS was not depending on the number of occluding pairs in denture than non-denture group. The average FraS was significantly lower for the denture 3.3 (±SD 3.25) compared to the non-denture group 1.5 (±SD 0.70) mm, (p = 0.02). These changes were independent of gender, age, occluding pairs. On Kennedy classification; only the class 3 of Kennedy showed a masticatory efficiency by giving small fragments size with 0.75 (±SD 0.35 mm) (Table 4).
The aim of this study was to evaluate whether partial removable denture use indeed leads to improved oral health related quality of life and masticatory function. The present study found that the oral health related quality of life was best in the non-denture- group than the denture-group (p = 0.00).
The parameters of functional limitation, pain, and physical disability showed a significant difference between both groups of participants, but the non-denture-group were more satisfied with their partial edentulous condition not paired than the denture- group (Table 2). Our result was similar with other author who showed that the wearing partial removable denture acrylic resin decreased the quality of life of beneficiary  .
Table 2. Oral health impact profile (OHIP).
According to  , the number of pair occluding or functional dental unit plays a very important role in the conservation of the masticatory performance of patients with partial removable denture.
This statement is in agreement with the results of the present study, which showed
Table 3. Masticatory parameters according to pairs occluding in both groups.
Table 4. Distribution of masticatory parameters in different class of Kennedy.
that the participants in the denture-group having at least two functional units had a good masticatory performance than the non-denture-group (Table 3). These results are also likewise with the studies of others authors. However, Tsakos G. et al. have reported that the number of occluding pairs is more related to the quality of oral health as the number of natural teeth  . In contrary, it was found by Sheiham A. et al. that it was difficult to establish a relationship between intake of nutrients and the number and distribution of teeth in people over 20 natural teeth  . They concluded that, the intake of dietary fiber was higher in people with more teeth and significantly associated with the number of occluding pairs. But, it’s also been demonstrated by other authors that, the number of occluding pairs units controls the masticatory efficacy    , which is similar to our results. With gender, a significant difference was found between two groups (p = 0.02 and p < 0.00), and men had much higher mastication performance than women (Table 1). These results are similar to those observed in previous studies   , who reported that the men have higher index efficacy than women.
The number of masticatory cycles in the denture-group was higher than that in the non-denture-group. These results are valid for the gender; age, occluding pairs units and type of classification of Kennedy (Table 1, Table 4). For the same food, the number of cycles before swallowing is characterized by a high inters individual variability within the same group of participants. This study shows that the patients in the denture-group work an average of 119.05 ± 53.70 cycles before swallowing (Table 3). This variability is also observed in the non-denture-group. The increase in the number of cycles thus appears to be a compensatory mechanism of the mastication disability. These results are similar to those     . The variations of the number of cycles can be explained by intrinsic and extrinsic factors. So, depending on the personality, environment and circumstance.
The frequency of mastication in the denture-group was conducted an average of 1.38 (±SD 0.25) Hertz and with 1.29 (±SD 0.15) Hertz for the non-denture-group (Table 1). The incidence of mastication was higher in the denture-group. Those results are similar to the studies of Bessadet M et al. and study of Sanchez-Ayala Aetal   .
Hope to have a larger size of sample and from different regions than used in this study and the changes addresses and telephone numbers of patients, and a few deaths cases were among the limitations of our work.
There are significant differences in oral health related quality of life between the denture and the non-denture group. The overall oral health related quality of life was satisfied in the non-denture than denture. Denture wearing participant displayed significantly more mastication cycles and needed more mastication time, which resulted in significantly smaller peanut fragment sizes compared to the non-denture wearing participants.
The authors would like to thank Pr. Dr. Fons Verdonck, Frans Vinckier, Mvumvi Le lo and Dr. Phillippe Vuylesteke from KU Leuven University and from Kinshasa University for their relevant scientific contribution and financial.
Conflict of Interest
The authors confirm that this article content has no conflict of interest.
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