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 JBM  Vol.4 No.10 , October 2016
The Modulatory Effect of Dietary Apostichopus japonicus on Mice with Ulcerative Colitis Induced by Trinitrobenzene Sulfonic Acid
Abstract: Sea cucumber is a food with nutritional benefits distributing mainly in Asia, and Apostichopus japonicus (A. japonicus) is a kind of sea cucumber whose quality is better than any others. However, different processing methods make various effect on its quality. In this study, we evaluated the protection effect of A. japonicus with different processing methods on mice with ulcerative colitis induced by trinitrobenzene sulfonic acid (TNBS), especially on the intestinal microflora. The expression of IFN-γ/IL-4 and IL-1β in gut, and intestinal microbiota were discussed. The results revealed that three different processing methods of A. japonicus could decrease the expression of inflammatory cytokines, except for the expression of IFN-γ/IL-4 treated with enzymatic, and dried A. japonicus was the most efficient. A. japonicus could change the microbiotic imblance relatively back to normal in terms of bacterial diversity and composition, meanwhile increase the abundance of Bifidobacteria, Lactobacillus and Clostridium leptum. The elements of protein, polysaccharide in dried, instant, enzymatic A. japonicus are 73.09%, 65.06%, 57.42% and 6.72%, 5.46%, 5.45% respectively. This study indicated that A. japonicus have a good improving effect on ulcerative colitis, especially on the microbiome, and processing methods had an effect on alleviation of ulcerative colitis, which might be associated with content of protein and polysaccharide.
Cite this paper: Shi, H. , Sun, H. , Zheng, R. , Lu, S. , Liu, F. , Zhang, N. , Xue, C. and Tang, Q. (2016) The Modulatory Effect of Dietary Apostichopus japonicus on Mice with Ulcerative Colitis Induced by Trinitrobenzene Sulfonic Acid. Journal of Biosciences and Medicines, 4, 15-27. doi: 10.4236/jbm.2016.410003.
References

[1]   Ordás, I., Eckmann, L., Talamini, M., et al. (2011) Ulcerative Colitis. Lancet, 380, 1606-1619.
http://dx.doi.org/10.1016/S0140-6736(12)60150-0

[2]   Qiu, W., Wu, B., Wang, X., et al. (2011) PUM, A-Mediated Intestinal Epithelial Apoptosis Contributes to Ulcerative Colitis in Humans and Mice. Journal of Clinical Investigation, 121, 1722-1732.
http://dx.doi.org/10.1172/JCI42917

[3]   Singh, U.P., Singh, N.P., Murphy, E.A., et al. (2015) Chemokine and Cytokine Levels in Inflammatory Bowel Disease Patients. Cytokine, 77, 44-49.
http://dx.doi.org/10.1016/j.cyto.2015.10.008

[4]   Zhang, M., et al. (2006) Curcumin Regulated Shift from Th1 to Th2 in Trinitrobenzene Sulphonic-Induced Chronic Colitis. Acta Pharmacologica Sinica, 27, 1071-1077.
http://dx.doi.org/10.1111/j.1745-7254.2006.00322.x

[5]   Lv, Q., Qiao, S.M., Xia, Y., et al. (2015) Norisoboldine Ameliorates DSS-Induced Ulcerative Colitis in Mice through Induction of Regulatory T Cells in Colons. International Immunopharmacology, 29, 787-797.
http://dx.doi.org/10.1016/j.intimp.2015.08.040

[6]   Bai, A.P., Lu, N.H., Zeng, H., et al. (2010) All-Trans Retinoic Acid Ameliorates Trinitrobenzene Sulfonic Acid-Induced Colitis by Shifting Th1 to Th2 Profile. Journal of Interferon & Cytokine Research, 30, 399-406.
http://dx.doi.org/10.1089/jir.2009.0028

[7]   Uronis, J.M., Arthur, J.C., Temitope, K., et al. (2011) Gut microbial Diversity Is Reduced by the Probiotic VSL#3 and Correlates with Decreased TNBS-Induced Colitis. Inflammatory Bowel Diseases, 17, 289-297.
http://dx.doi.org/10.1002/ibd.21366

[8]   Rosa, K.B., Zehra, E.I., et al. (2012) Effects of Gut Microbes on Nutrient Absorption and Energy Regulation. Nutrition in Clinical Practice, 27, 201-214.
http://dx.doi.org/10.1177/0884533611436116

[9]   Morgan, X.C., Tickle, T.L., Sokol, H., et al. (2012) Dysfunction of the Intestinal Microbiome in Inflammatory Bowel Disease and Treatment. Genome Biology, 13, 1-18.
http://dx.doi.org/10.1186/gb-2012-13-9-r79

[10]   Wang, W., Chen, L.P., Zhou, R., et al. (2014) Increased Proportions of Bifidobacterium and the Lactobacillus Group and Loss of Butyrate-Producing Bacteria in Inflammatory Bowel Disease. Journal of Clinical Microbiology, 52, 398-406.
http://dx.doi.org/10.1128/JCM.01500-13

[11]   Kabeerdoss, J., Sankaran, V., et al. (2013) Clostridium leptum Group Bacteria Abundance and Diversity in the Fecal Microbiota of Patients with Inflammatory Bowel Disease: A Case-Control Study in India. BMC Gastroenterology, 13, 1-8.
http://dx.doi.org/10.1186/1471-230X-13-20

[12]   Zang, K.H., Rao, Z., et al. (2015) Anticolitis Activity of Chinese Herbal Formula Yupingfeng Powder via Regulating Colonic Enterochromaffin Cells and Serotonin. Indian Journal of Pharmacology, 47, 632-637.
http://dx.doi.org/10.4103/0253-7613.169584

[13]   Zheng, R., Li, X.M., Cao, B.B., et al. (2014) Dietary Apostichopus Japonicus Enhances the Respiratory and Intestinal Mucosal Immunity in Immunosuppressive Mice. Bioscience Biotechnology & Biochemistry, 79, 253-259.
http://dx.doi.org/10.1080/09168451.2014.955454

[14]   Xue, C.H., Xue, Y., Wang, J.F., et al. (2007) Sea Cucumber Nutrient and Its Preparing Process. 2007 CN 101016506 A.

[15]   Otsuka, M., Kang, Y.J., Ren, J.L., et al. (2010) Distinct Effects of P38α Deletion in Myeloid Lineage and Gut Epithelia in Mouse Models of Inflammatory Bowel Disease. Gastroenterology, 138, 1255-1265.
http://dx.doi.org/10.1053/j.gastro.2010.01.005

[16]   Zhou, W., Cao, Q., Peng, Y., et al. (2009) FoxO4 Inhibits NF-κB and Protects Mice against Colonic Injury and Inflammation-Gastroenterology. Gastroenterology, 137, 1403-1414.
http://dx.doi.org/10.1053/j.gastro.2009.06.049

[17]   Aline, W., Luchini, A.C., Hiruma-Lima, C.A., et al. (2012) Suppression of TNBS-Induced Colitis in Rats by 4-Methylesculetin, a Natural Coumarin: Comparison with Prednisolone and Sulphasalazine. Chemico-Biological Interactions, 195, 76-85.
http://dx.doi.org/10.1016/j.cbi.2011.11.004

[18]   Brian, C., Violeta, Z., et al. (2015) Effect of Genetic Deletion or Pharmacological Antagonism of Tumor Necrosis Factor Alpha on Colitis-Associated Carcinogenesis in Mice. Inflammatory Bowel Diseases, 21, 485-495.
http://dx.doi.org/10.1097/MIB.0000000000000303

[19]   Inagaki-Ohara, K., Sasaki, A., Matsuzaki, G., et al. (2006) Suppressor of Cytokine Signalling 1 in Lymphocytes Regulates the Development of Intestinal Inflammation in Mice. Gut, 55, 212-219.
http://dx.doi.org/10.1136/gut.2004.062653

[20]   Pender, S.L., Chance, V., Whiting, C.V., et al. (2005) Systemic Administration of the Chemokine Macrophage Inflammatory Protein 1α Exacerbates Inflammatory Bowel Disease in a Mouse Model. Gut, 54, 1114-1120.
http://dx.doi.org/10.1136/gut.2004.052779

[21]   Doré, J. and Blottière, H. (2015) The Influence of Diet on the Gut Microbiota and Its Consequences for Health. Current Opinion in Biotechnology, 32, 195-199.
http://dx.doi.org/10.1016/j.copbio.2015.01.002

[22]   Tuohy, K.M., Hinton, D.J.S., Davies, S.J., et al. (2006) Metabolism of Maillard Reaction Products by the Human Gut Microbiota Implications for Health. Molecular Nutrition & Food Research, 50, 847-857.
http://dx.doi.org/10.1002/mnfr.200500126

[23]   Tojo, R., Suárez, A., Clemente, M.G., et al. (2014) Intestinal Microbiota in Health and Disease: Role of Bifidobacteria in Gut Homeostasis. World Journal of Gastroenterology, 20, 15163-15176.
http://dx.doi.org/10.3748/wjg.v20.i41.15163

[24]   Eckburg, P.B., Bik, E.M., Bernstein, C.N., et al. (2005) Diversity of the Human Intestinal Microbial Flora. Science, 308, 1635-1638.
http://dx.doi.org/10.1126/science.1110591

[25]   Wichmann, A., Allahyar, A., Greiner, T.U., et al. (2013) Microbial Modulation of enerGy Availability in the Colon Regulates Intestinal Transit. Cell Host & Microbe, 14, 582-590.
http://dx.doi.org/10.1016/j.chom.2013.09.012

[26]   Hamer, H.M., Jonkers, D., et al. (2008) The Role of Butyrate on Colonic Function. Alimentary Pharmacology & Therapeutics, 27, 104-119.
http://dx.doi.org/10.1111/j.1365-2036.2007.03562.x

[27]   Tremaroli, V. and Backhed, F. (2012) Functional Interactions between the Gut Microbiota and Host metabolism. Nature, 489, 242-249.
http://dx.doi.org/10.1038/nature11552

[28]   Mariadason, J.M., Catto-Smith, A. and Gibson, P.R. (1999) Modulation of Distal Colonic Epithelial Barrier Function by Dietary Fibre in Normal Rats. Gut, 44, 394-399.
http://dx.doi.org/10.1136/gut.44.3.394

[29]   Josefowicz, S.Z., Niec, R.E., Hye Young, K., et al. (2012) Extrathymically Generated Regulatory T Cells Control Mucosal Th2 Inflammation. Nature, 482, 395-399.
http://dx.doi.org/10.1038/nature10772

[30]   Nicholas, A., Clarissa, C., Xiying, F., et al. (2013) Metabolites Produced by Commensal Bacteria Promote Peripheral Regulatory T Cell Generation. Nature, 504, 451-455.
http://dx.doi.org/10.1038/nature12726

[31]   Honda, K. and Littman, D.R. (2012) The Microbiome in Infectious Disease and Inflammation. Annual Review of Immunology, 30, 759-795.
http://dx.doi.org/10.1146/annurev-immunol-020711-074937

 
 
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