AJPS  Vol.7 No.14 , October 2016
Identification of Leaf Based Physiological Markers for Drought Susceptibility during Early Seedling Development of Mungbean
Abstract: Drought is a recurrent phenomenon in many of the countries. Moisture stress during seedling stage is very critical in determining the establishment of the crop and its further development and yield. Identifying drought tolerance mechanism and physiological markers of drought susceptibility is this crop during seedling stress would be useful tool in future genetic manipulation programme to establish drought tolerance in this crop. Thus the present study aimed for quickly identifying reliable physiological markers for drought susceptibility through evaluation of physiological and biochemical changes in leaves of two contrasting mungbean (Vigna radiata L. Wilczek) cultivars i.e. K 851 (drought tolerant) and PDM 84-139 (drought susceptible) during seedling development. A range of four external water potentials (i.e. -1.0, -2.0, -3.0 and -4.0 bars), besides glass distilled water as control (0.0 bar), was used. Parameters like leaf area, relative leaf water content, chlorophyll content, chlorophyll stability indices in both the cultivars were decreased with the increasing magnitude of stress. By and large phenols and ascorbic acid content were increased with the stress level but the trend was not consistent. A steady rise in proline, hydrogen peroxide content and lipid peroxidation was found with water stress. Out of two cultivars tested, drought tolerant cultivar K 851 was better in leaf water balance and higher accumulation of phenols, proline and ascorbic acid than PDM 84-139. The correlation study indicated lipid peroxidation and H2O2 content as valuable physiological markers for screening of drought susceptibility.
Cite this paper: Dutta, P. , Bandopadhyay, P. and Bera, A. (2016) Identification of Leaf Based Physiological Markers for Drought Susceptibility during Early Seedling Development of Mungbean. American Journal of Plant Sciences, 7, 1921-1936. doi: 10.4236/ajps.2016.714176.

[1]   Bohnert, H.J. and Sheveleva, E. (1998) Plant Stress Adaptations—Making Metabolism Move. Current Opinion in Plant Biology, 1, 267-274.

[2]   Mahajan, S. and Tuteja, N. (2005) Cold, Salinity and Drought Stresses: An Overview. Biochem Biophys, 444, 139-158.

[3]   Allahmoradi, P., Ghobadi, M. and Taherabadi, S. (2011) Physiological Aspects of Mungbean in Response to Drought Stress. International Conference on Food Engineering and Biotechnology, 9, 272-275.

[4]   Patade, V.Y., Maya, K. and Zakwan, A. (2011) Seed Priming Mediated Germination Improvement and Tolerance to Subsequent Exposure to Cold and Salt Stress in Capsicum. Research Journal of Seed Science, 4, 125-136.

[5]   Postel, S.L. (2000) Entering an Era of Water Scarcity. The Challenges Ahead. Ecological Application, 10, 941-948.[0941:EAEOWS]2.0.CO;2

[6]   Kramer, P.J. and Boyer, J.S. (1997) Water Relation in Plants and Soils. Academic Press, San Diego, Calif.

[7]   Chaves, M.M., Flexas, J. and Pinheiro, C. (2009) Photosynthesis under Drought and Salt Stress: Regulation Mechanisms from Whole Plant to Cell. Annals of Botany, 103, 551-560.

[8]   Nahar, K., Hasanuzzaman, M., Alam, Md.M. and Fujita, M. (2015) Glutathion-Induced Drought Stress Tolerance in Mungbean: Coordinated Roles of the Antioxidant Defence and Methyglyoxal Detoxification Systems. AoB Plants, 7, plv069.

[9]   Kaur, V., Singh, S. and Behl, R.K. (2016) Heat and Drought Tolerance in Wheat: Integration of Physiological and Genetic Platforms for Better Performance under Stress. Journal of Crop Breeding and Genetics, 2, 1-14.

[10]   Bogale, A., Tesfaye, K. and Geleto, T. (2011) Morphological and Physiological Attributes Associated to Drought Tolerance of Ethiopian Durum Wheat Genotypes under Water Deficit Condition. Journal of Biodiversity and Environmental Sciences, 1, 22-36.

[11]   Jallel, C.A., Manivannan, P., Wahid, A., Farook, M., Somasundaram, R. and Panneerselvam, R. (2009) Drought Stress in Plants: A Review on Morphological Characteristics and Pigments Compositions. International Journal of Agriculture and Biology, 11, 100-105.

[12]   Razmjoo, K., Heidarizahed, P. and Sabzalian, M.R. (2008) Effect and Salinity and Drought Stresses on Growth Parameters and Essential Oil Content of Matricaria Chamomile. International Journal of Agriculture and Biology, 10, 451-454.

[13]   Giancarla, V., Madosa, E., Sumalan, R., Adriana, C. and Cerasela, P. (2012) Evaluation of Some Indirect Indices to Identify Drought Tolerance in Barley. Journal of Horticulture, Forestry and Biotechnology, 16, 239-241.

[14]   Dutta, P. and Bera, A.K. (2008) Screening of Mungbean Genotypes for Drought Tolerance. Legume Research, 31, 145-148.

[15]   Michel, B.E. and Kaufmann, M.R. (1973) The Osmotic Potential of Polyethylene Glycol 6000. Plant Physiology, 51, 914-916.

[16]   Nandi, S. and Bera, A.K. (1995) Effect of Mercury and Manganese on Seed Germination and Seedling Growth in Black Gram. Seed Research, 23, 125-128.

[17]   Maiti, R.K., de la Rosa-Ibarra, M. and Sandoval, N.D. (1994) Genotypic Variability in Glossy Sorghum Lines for Resistance to Drought, Salinity and Temperature Stress at the Seedling Stage. Plant Physiology, 143, 241-244.

[18]   Barrs, H.D. and Weatherley, P.E. (1962) A Re-Examination of the Relative Turgidity Techniques for Estimating Water Deficits in Leaves. Australian Journal of Biological Sciences, 15, 413-428.

[19]   Hiscox, J.D. and Israelstam, G.F. (1978) A Method for the Extraction of Chlorophyll from Leaf Tissue without Maceration. Canadian Journal of Botany, 57, 1332-1334.

[20]   Arnon, D.I. (1949) Copper Enzymes in Isolated Chloroplasts. Polyphenol Oxidase in Beta Vulgaris. Plant Physiology, 24, 1-15.

[21]   Sairam, R.K. (1994) Effect of Moisture Stress on Physiological Activities of Two Contrasting Wheat Genotypes. Indian Journal of Experimental Biology, 32, 594-597.

[22]   Hodges, D.M., DeLong, J.M., Forney, C.F. and Prange, R.K. (1999) Improving the Thiobarbituric Acid-Reactive Substances Assay for Estimating Lipid Peroxidation in Plant Tissues Containing Anthocyanine and other Interfering Compounds. Planta, 207, 604-611.

[23]   Bates, L.S., Waldren, R.P. and Teare, I.D. (1973) Rapid Determination of Free Proline for Water Stress Studies. Plant and Soil, 39, 205-207.

[24]   Swain, J. and Hillis, W.E. (1959) The Phenolic Constitutents of Prunus domestica. I. The Quantitative Analysis of Phenolic Constituents. Journal of the Science of Food and Agriculture, 10, 63-68.

[25]   Mukherjee, S.P. and Choudhuri, M.A. (1983) Implications of Water Stress Induced Changes in the Levels of Endogenous Ascorbic Acid and Hydrogen Peroxide in Vigna Seedlings. Physiologia Plantarum, 58, 166-170.

[26]   Teranishi, Y., Tanaka, A., Osumi, M. and Fukui, S. (1974) Catalase Activity of hyDrocarbon Utilizing Candida Yeast. Agricultural Biological Chemistry, 38, 1213-1216.

[27]   Panse, V.G. and Sukhatme, P.V. (1989) Statistical Methods for Agricultural Workers. ICAR, New Delhi.

[28]   Thakur, P.S. and Rai, V.K. (1984) Water Stress Effects on Maize: Growth Responses of Two Differentially Drought Sensitive Maize Cultivars during Early Stage of Growth. Indian Journal of Ecology, 11, 92-98.

[29]   Baalbaki, R.Z., Zurayk, R.A., Blaik, M.M. and Talhouk, S.N. (1999) Germination and Seedling Development of Drought Tolerant and Susceptible Wheat under Moisture Stress. Seed Science and Technology, 27, 291-302.

[30]   De, R., Sinhababu, A., Banerjee, A. and Kar, R.K. (2005) Effect of Water Stress on Seed Germination and Seedling Growth in Mungbean and Blackgram. Crop Research, 29, 148-155.

[31]   Boyer, J.C. (1983) Sub-Cellular Mechanism of Plant Response to Low Water Potential. Agricultural Water Management, 7, 239-248.

[32]   Naidu, T.C.M., Raju, N. and Narayanan, A. (2001) Screening of Drought Tolerance in Green Gram (Vigna radiata L. Wilczek) Genotypes under Receding Soil Moisture. Indian Journal of Plant Physiology, 6, 197-201.

[33]   Deshmukh, D.V., Mhase, L.B. and Jamadagni, B.M. (2004) Evaluation of Chickpea Genotypes for Drought Tolerance. Indian Journal of Pulses Research, 17, 47-49.

[34]   Virk, S.S. and Singh, O.S. (1990) Osmotic Properties of Drought Stressed Periwinkle (Chatharanthus roseus) Genotypes. Annals of Botany, 66, 23-30.

[35]   Reddy, M.P. and Vora, A.B. (1986) Salinity Induced Changes in Pigment Composition and Chlorophyllase Activity of Wheat. Indian Journal of Plant Physiology, 29, 331-334.

[36]   Reddy, A.M., Shankhdhar, D. and Shankhdhar, S.C. (2007) Physiological Characterization of Rice Genotypes under Periodic Water Stress. Indian Journal of Plant Physiology, 12, 89-93.

[37]   Hayatu, M. and Mukhtar, F.B. (2010) Physiological Responses of Some Drought Resistant Cowpea Genotypes (Vigna unguiculata L Walp) to Water Stress. Bayero Journal of Pure and Applied Sciences, 3, 69-75.

[38]   Kumari, M., Dass, S., Vimala, Y. and Arora, P. (2004) Physiological Parameters Governing Drought Tolerance in Maize. Indian Journal of Plant Physiology, 9, 203-207.

[39]   Dutta, P. and Bera, A.K. (2014) Effect of NaCl Salinity on Seed Germination and Seedling Growth of Mungbean Cultivars. Legume Research, 37, 161-164.

[40]   Anaytullah, Bose, B. and Yadav, R.S. (2007) PEG Induced Moisture Stress: Screening for Drought Tolerance in Rice. Indian Journal of Plant Physiology, 12, 189-192.

[41]   Sivakumar, V., Ramchandran, K., Ravichandran, V. and Vangamud, M. (1998) Effect of Drought Hardening on Proline Content of the Tree Seedlings. Annals of Plant Physiology, 12, 82-84.

[42]   Dutta, P. and Bera, A.K. (2007) Oxidative Stress and Changes in the Activity of Active Oxygen Scavenging Enzymes of Mungbean Seedling Subjected to Water Stress. Indian Journal of Plant Physiology, 12, 199-210.

[43]   Sairam, R.K. and Srivastava, G.C. (2001) Water Stress Tolerance of Wheat (Triticum aestivum L.): Variations in Hydrogen Peroxide Accumulation and Antioxidant Activity in Tolerant and Susceptible Genotypes. Journal of Agronomy and Crop Science, 186, 63-70.

[44]   Ranieri, A., Lencioni, L., Schenone, G. and Franco, G. (1993) Glutathione-Ascorbic Acid Cycle Pump in Plants Grown under Polluted Air in Open Top Chambers. Plant Physiology, 142, 286-290.

[45]   Sairam, R.K., Deshmukh, P.S. and Saxena, D.C. (1998) Role of Antioxidant System in Wheat Genotypes’ Tolerance to Water Stress. Biologia Plantarum, 41, 384-394.

[46]   Hahlbrock, K. and Scheel, D. (1989) Physiology and Molecular Biology of Phenylpropanoid Metabolism. Annual Review of Plant Physiology and Plant Molecular Biology, 40, 347-369.

[47]   Das, N., Misra, M. and Misra, A.N. (1990) Sodium Chloride Salt Stress Induced Metabolic Changes in Callus Cultures of Pearlmillet (Pennisetum americanum L. Leeke): Free Solute Accumulation. Plant Physiology, 137, 244-246.

[48]   Bor, M., Ozdemir, F. and Turkur, I. (2003) The Effect of Salt Stress on Lipid Peroxidation and Antioxidants in Leaves of Sugarbeet (Beta vulgaris L.) and Wildbeat (Beta meritima L.). Plant Science, 164, 77-84.

[49]   Ding, Z.S., Zhou, B.Y., Sun, X.F. and Zhao, M. (2012) High Light Tolerance Is Enhanced by Overexpressed PEPC in Rice under Drought Stress. Acta Agronomica Sinica, 38, 285-292.

[50]   Silva, E.N., Ribeiro, R.V., Ferreira-Silva, S.L., Vieira, S.A., Ponte, L.F.A. and Silveira, J.A.G. (2012) Coordinate Changes in Photosynthesis, Sugar Accumulation and Antioxidative Enzymes Improve the Performance of Jatropha curcas Plants under Drought Stress. Biomass and Bioenergy, 45, 270-279.