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 OJCE  Vol.6 No.4 , September 2016
Characterization of Mould in Masonry in Hospital Environment—Case Study
Abstract: The objective of this study is the characterization of mould inside and in the surface wall of a hospital. The present research was made on the wall of the Clinical Hospital of the Federal University of Paraná, Brazil. For the methodology the samples were extracted from the surface, mortar and brick. The samples were spread on Petri plates containing Sabouraud dextrose agar and incubated at 25 for seven days. The results of the 90 samples collected showed growth of 39% of colonies with the following distribution of microorganisms: Aspergillus (present in 27% of samples), Cladosporium, Absidia, Rhizopus, Rhodotorula, Fusarium, Penicillium and Aspergillus flavus. Within the investigated substrate, three species of different fungi were identified: Aspergillus flavus, Aspergillus fumigatus and Aspergillus niger.
Cite this paper: de Souza, W. and Matoski, A. (2016) Characterization of Mould in Masonry in Hospital Environment—Case Study. Open Journal of Civil Engineering, 6, 618-630. doi: 10.4236/ojce.2016.64050.
References

[1]   Zanon, U. and Neves, J. (1987) Infecções Hospitalares—Prevenção, Diagnóstico e Tratamento. MEDSI, Rio de Janeiro.

[2]   Del Palacio, A., Cuetara, M.S. and Ponton, J. (2003) Invasive Aspergillosis. Revista Iberoamericana de Micología, 20, 77-78.

[3]   Bhabhra, R. and Askew, D.S. (2005) Thermotolerance and Virulence of Aspergillus fumigatus: Role of the Fungal Nucleolus. Medical Mycology, 43, 87-93.
http://dx.doi.org/10.1080/13693780400029486

[4]   Curtis, L., Cali, S., Conroy, L., Baker, K., Ou, C.H., Hershow, R., Norlock-Cruz, F. and Scheff, P. (2005) Aspergillus Surveillance Project at a Large Tertiary-Care Hospital. Journal of Hospital Infection, 53, 188-196.
http://dx.doi.org/10.1016/j.jhin.2004.05.017

[5]   Ranjana, K.H., Priyokumar, K., Singh, T.J., Gupta, C.C., Sharmila, L., Singh, P.N. and Charkrabartl, A. (2002) Disseminated Penicillium marneffei Infection among HIV-Infected Patients in Manipur State, India. Journal of Infection, 45, 268-271.
http://dx.doi.org/10.1053/jinf.2002.1062

[6]   Richardson, M.D. and Warnock, D.W. (2003) Fungal Infection Diagnosis and Management. 3rd Edition, Blackwell Publishing Ltd., Oxford.

[7]   Singh, J., Yu, C.W.F. and Kim, J.T. (2010) Building Pathology, Investigation of Sick Buildings—Toxic Moulds. International Society of the Built Environment, Londres.

[8]   Shirakawa, M.A., Beech, I.B., Tapper, R., Cincotto, M.A. and Gambale, W. (2003) The Development of a Method to Evaluate Bioreceptivity of Indoor Mortar Plastering to Fungal Growth. International Biodeterioration & Biodegradation, 51, 83-92.
http://dx.doi.org/10.1016/S0964-8305(01)00129-9

[9]   ANVISA (Agência Nacional de Vigilância Sanitária Brasileira) (2003) Padrão Brasileiro de Microbiologia-Resolução 09/2003. Diário Oficial da União, Brasília.

[10]   NT-SCE-02. Nota Técnica Portuguesa (2006) Metodologia para auditorias periódicas de qualidade do ar interior em edifícios de serviços existentes no âmbito do RSECE, Portugal.

[11]   American Public Health Association (2012) Microbiological Examination of Water. In: Standard Methods for the Examination of Water and Wastewater, Method 9215-B, 22nd Edition, American Public Health Association, Washington DC.

[12]   American Society for Testing and Materials (2015) ASTM D7234-12: Standard Test Method for Pull-Off Adhesion Strength of Coatings on Concrete Using Portable Pull-Off Adhesion Testers.

[13]   Merck (2014) Air MAS-100: Microbiological Air Sample-Operator’s Manual. Darmstadt, Germany, p. 47.

[14]   (1991) NE 01-90, Guide du Bionettoyage. Official Journal French Republic, France.

 
 
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