Health  Vol.1 No.2 , September 2009
An ion-based chromogenic method of detecting for inorganic phosphate in serum and milk
Abstract: A rapid method for the determination of inor-ganic phosphate in serum and milk by an ion-based chromogenic is described. Serum samples were detected directly by our system, and milk was also detected after degreased through centrifugation. By this procedure the samples are not diluted. Mean serum inorganic phosphate concentration found in healthy indi-vidual is 1.14mmol/L. Values found in serum is in good agreement with those previously re-ported. Mean inorganic phosphate concentra-tion from foremilk and commercial milk are 2.5mmol/L and 12.5mmol/L respectively.
Cite this paper: nullYin, C. , Su, J. , Huo, F. and Yang, P. (2009) An ion-based chromogenic method of detecting for inorganic phosphate in serum and milk. Health, 1, 76-82. doi: 10.4236/health.2009.12013.

[1]   Grases, F. and March, J. G. (1990) Determination of phosphate based on inhibition of crystalline growth of calcite. Analytica Chimica Acta., 229, 249-254.

[2]   Ender, D. B. and Rude, R. K., (1999) Mineral and bone metabolism, In Tietz Textbook of Clinical Chemistry (C. A. Burtis, E. R. Ashwood, Eds.), W. B. Saunders, com-pany: P. A. Philadelphia, 1406-1408,1439-1440.

[3]   Kestenbaum, B., Sampson, J. N., Rudser, K. D., et al. (2005) Characterization of phosphate transport in rat vascular smooth muscle cells. J Am Soc Nephrol., 16, 520-528.

[4]   Levi, M. and Knochel, J. P. (1990) The management of disorders of phosphate metabolism. In Therapy of Renal Diseases and Related Disorders (S. G. Massry, W. N. Suki,), Boston, Martinus Nijhoff.

[5]   Levi, M., Cronin, R. E., and Knochel, J. P. (1992) Disor-ders of phosphate and magnesium metabolism. In Disor-ders of Bone and Mineral Metabolism (F. L. Coe, M. J Favus,), New York: Raven Press.

[6]   Biochrom Ltd Certificate No: 890333 Determination of phosphate in clinical samples.

[7]   Popovtzer, M., Knochel, J. P., and Kumar, R. (1997) Disorders of calcium, phosphorus,vitamin D, and para-thyroid hormone activity. In Renal Electrolyte Disorders, edn 5 (R. W. Schrier,) Philadelphia: Lippincott-Raven.

[8]   Hruska, K. and Gupta, A. (1998) Disorders of phosphate homeostasis. In Metabolic Bone Disease, edn 3 (L. V. Avioli, S. M. Krane,) New York: Academic Press.

[9]   Greer, F. R., Steichen, J. J., and Tsang, R. C., (1982) Calcium and. phosphate supplements in breast milk- re-lated. Rickets. Am J Dis Child., 136, 581-583.

[10]   Fiske, C. H. and Subbarow, Y. J. (1925) The colorimetric determination of phosphorus. Biol. Chem., 66, 375-400.

[11]   Lowry, O. H. and Lopez, J. A. (1946) The determination of inorganic phosphate in the presence of labile phos-phate ester. J. Biol. Chem., 182, 421.

[12]   Bartles, P. C. and Roijers, A. F. M. (1975) A kinetic study on the influence ofparameters in the determination of in-organic phosphate by the molybdenum blue reaction. Clin. Chim. Acta., 61, 153-4.

[13]   Daly, J. A. and Ertingshausen, G. (1972) Direct method for determining inorganic phosphorus in serum. with the Centrifichem. Clin. Chem., 18, 263-265.

[14]   Urban, A. E. (1972) Simplified. serum phosphorus. analyses. by continuous-flow. ultraviolet. Spectropho-tometry. J. Clin. Chem., 18, 601-4.

[15]   Fogg, A. G., Soleymanloo, S., and Burns, D. T. (1977) The spectrophotometric determination of inorganic phosphate in biological system with crystal violet. Anal. Chim. Acta., 88, 197-200.

[16]   Endres, B. D. and Rude, R. K. (1994) Mineral and bone metabolism. Tietz textbook of clinical chemistry (C. A. Burtis, E. R. Ashwood, eds.), Philadelphia: W. B. Saun-ders, Co., 1887-973.

[17]   Yin, C. X., Huo F. J., and Yang, P. (2005) An ion-based chromogenic detecting method for phosphate-containing derivatives in physiological condition. Sensors and Ac-tuators B Chemical., 109, 291–299.

[18]   Yin, C. X., Huo, F. J., Yang, P. (2006) UV-Vis spectro-scopic study directly detecting inorganic phosphorus in urine and our reagent kit. Anal Bioanal Chem., 384, 774-779.