ABC  Vol.6 No.4 , August 2016
A Model of Hormonal and Environmental Involvement in Growth and Sex Differentiation in European Eel (Anguilla Anguilla)
Author(s) Gad Degani
ABSTRACT
Growth in vertebrates is a complex interaction involving the development of cells, tissues and organs. Hormones regulating growth during maturation are involved mainly in the interaction between the somatic axis and the reproduction of brain-pituitary axes. Based on the results of hormone and gene transcriptions, the secretion and treatment controlling both the somatic axis and the gonadotropic axis were affected, directly or indirectly, by the environment parameter through hormones that were studied in my laboratory. A model was proposed for sex differentiation and gonadal development correlating to the growth of European eel (Anguilla anguilla). A high growth variation is affected by the environment. At a low density of eels, the gonadotropin-releasing hormone (GnRH) affected the secretion follicle stimulation hormone (FSH) in the pituitary, steroidogenesis and aromatase (CYP19) synthesis, and the 17β-estradiol (E2) from 11-ketotestosterone (Kt-11) causing ovary development. The ovary secretion E2 affecting the ade-nylate cyclase-activating polypeptide (PACAP), growth hormone (GH) and the insulin-like growth factor (IGF) stimulated rapid growth in females. On the other hand, a high density of eels caused the pituitary gland to secrete FSH at a lower level, and CYP19 was not synthesized in the gonads. The secretion of Kt-11 affected differentiation to testis, which inhibits the somatic axis in reducing growth rate.

Received 1 August 2016; accepted 23 August 2016; published 26 August 2016

1. Introduction

European eel (Anguilla anguilla) is a teleost species having a complex lifecycle both in sea and freshwater environments [1] . High variation in growth was discovered in European eel grown under aquacultural conditions [2] , [3] . Under natural conditions, many biological aspects of European eel were studied, including lifecycle, spawning behavior, migration, growth and metamorphosis from larvae to mature. The sex ratio in silver eels varies considerably between localities, ranging between 0% to 100% for females [1] , adults [4] - [8] . The lifecycle, evolution and reproduction of European eel were reviewed in detail [9] . Many aspects of eel growth and nutrition were described under artificial conditions, which have been reviewed and summarized [10] (Figure 1).

Significant differences between growth rate and size have been observed between the two sexes, with females growing faster and larger than males [11] . The environment has been found to affect growth and sex determination in eels; the females continue to grow, while the males cease to do so. Some males weigh between 15 - 200 g. Many theoretical and applied aspects of European eel, including hormone involvement in growth and reproduction, have been investigated [10] . The administration of various hormones in eel diets influences growth [12] - [14] . In European eel, molecular cloning of the PACAP precursor has been previously shown in fish, PACAP and GH-releasing hormone (GHRH) originating from the same precursor. The effects of PACAP and GHRH on GH secretion from eel pituitary cells in primary culture have been described [15] . The growth hormone sequence analysis and transcription of European eel were studied [11] , as well as gonadotropins, follicle-stimulating hormone (FSH) and luteinizing hormone (LH) [11] . Japanese eel (Anguilla japonica) (Kazeto et al. 2008 [16] ), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) produce biologically active recombinant FSH and LH by Drosophila S2 cells and have differential actions on reproductive biology (Aroua et al. 2012 [17] ). Plasma progesterone and estradiol increase after heterologous gonadotropin (hCG) treatment (Colombo et al. 1987 [18] ).

The effect of hormones on sex determination and growth by steroids [14] , [19] - [21] was studied in detail.

In this mini-review, a model is presented suggesting that the relationship between the environment, hormones and sex determination affects growth. The relevance of such a model, not only in providing basic information, but also with respect to aquaculture, is described.

1.1. Environmental Effect on Sex Differentiation and Growth of European Eel

The European eel is a catadromous and carnivorous species having a complex lifecycle in sea and freshwater [10] . The European eel spawn in the Sargasso Sea in late winter and spring [1] , [4] . The leaf-shaped larvae of European eel (leptocephali) [5] are brought to the continental shelf of Europe by the Gulf Stream.

Before the eel enter into the freshwater of rivers in Europe and Mediterranean countries, the larvae metamorphose to glass eels. During periods in freshwater, they undergo growth and maturation, and change their pigments to yellow eels and silvery eels. Sex differentiation in eels occurs during the yellow eel stage [18] , [22] [23] . The eels become sexually mature at the silver eel stage. Young eels live in freshwater, where they stay for a period of 6 - 12 years for males and 9 - 18 years for females [1] . As silver eels become sexually mature, they swim back to the sea and spawning grounds in the Sargasso Sea [1] . Eel is an antic animal that lives in solitary in the ground [1] . Based on experimental growing of eel under artificial and aquacultural conditions, many data support the density effect on sex differentiation (Figure 1). European eels maintain high densities, with the percentage of males being significantly higher compared to females.

Under artificial conditions during growth, a very high variation in growth was found [2] , and the important environmental factor affecting growth was density [10] . The rapid-growth eels become females and the slow- growth eels become males [24] . The high density of growth significantly reduces growth, and estradiol increases

Figure 1. European eel grown under aquacultural conditions.

growth and the percentage of females in the population [24] . The relationship between environment and sex differentiation among males and females is presented in Figure 2.

The proposed model described in Figure 1 might be explained by a study on hormone control reproduction and growth. In other words, the effect of the environment is on the hormonal axis.

1.2. Hormonal Involvement in Sex Differentiation in Eel

European eels belong to teleosts, as in other vertebrates, and growth and reproduction are tightly regulated, mainly via the hypothalamus-pituitary axis [25] . The hypothalamic decapeptide, gonadotropin-releasing hormone (GnRH), controls the release of pituitary gonadotropins, follicle-stimulating hormone (FSH) and luteinizing hormone (LH), which, in turn, control gametogenesis [26] . It is becoming increasingly evident that growth hormone (GH) plays a role in reproduction [25] , [27] - [29] . A complex regulatory system involving multiple stimulatory and inhibitory factors may control the GH axis. In addition to its role in development and somatic growth, GH is involved in spermatogenesis regulation [11] .

Previous studies support the hypothesis that sex hormones affect growth in European eel. Moreover, the administration of 17β-estradiol (E2) in the diets affects growth more than 17a-methyltestosterone (MT) [30] . A high percentage (70%) of females was found among the European eels administered E2 (60 mg/Kg diet) [20] . This result is supported by other studies [21] , [31] . The hormone controlling the E2 is FSH. Cloned [17] cDNA of European eel and the RNA level of FSH were significantly higher than in males [32] . Moreover, the expression of aromatase (EeCYP19) gene during the process of sex determination in adult male gonads was lower when compared to the adult female group [21] , [31] .

On the other hand, the transcription of GH is significantly higher in female eels than in males [11] . In the model for the environmental effect on sex differentiation and growth (Figure 2), it is proposed that low density in the hypothalamus-pituitary-gonad axis is affected by high FSH [32] and gonad synthesis E2 by EeCYP19, and E2 affects gonad to ovary differentiation [21] , [31] .

Figure 2. Proposed model of the effect of environment and density on sex differentiation [2] , [10] , [11] , [14] , [19] - [21] .

Figure 3. The relationship between environment and hormones involved in the differentiation of gonads in European eel [11] , [13] , [14] , [17] - [21] , [24] .

E2 affects the increase in growth compared to testosterone [29] , and GH transcription in females was significantly higher than in males [11] . These results show the effect of E2 on GH in the model (Figure 2). Growth hormone is affected both directly by growth and the insulin-like growth factor (IGF) system in the liver. The administration if insulin in the European eel diet significant increases growth rate [33] . In Japanese eel (Anguilla japonica), insulin-like growth factor-II (IGF-II) cDNAs were cloned and their mRNA expression was examined in several tissues. Two eel IGF-II cDNAs, eIGF-II-1 and eIGF-II-2, were cloned from the liver [34] [35] .

Under high density conditions where European eel grow, the mRNA level of FSH was significantly lower in males [32] , and the inhibition to EeCYP19 significantly reduced the level of E2. Moreover, the expression of aromatase (EeCYP19) gene during the process of sex determination in adult male gonads was lower when compared to the adult female group [21] , [31] . Gonads that do not undergo differentiation do not change into ovaries, secrete 11-KT and very low E2 levels have a lesser effect on growth than ovaries [29] Figure 3.

Cite this paper
Degani, G. (2016) A Model of Hormonal and Environmental Involvement in Growth and Sex Differentiation in European Eel (Anguilla Anguilla). Advances in Biological Chemistry, 6, 141-145. doi: 10.4236/abc.2016.64012.
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