Received 15 January 2015; accepted 20 August 2016; published 23 August 2016
African star apple (Chrysophyllum albidum) fruit is a forest tree that belongs to the family Sapotaceae. Its natural occurrence has been reported in diverse ecozones in Nigeria, Uganda, Niger Republic, Cameroon and Cote d’Ivorie. The plant often grows to a height of 36.5 m though it may be smaller  . Its leaves are used in ethno-medicine  . The plant has in recent times become a crop of commercial value in Nigeria. The fleshy pulp of the fruits is eaten especially as snacks and relished by both young and old  . The fruit has been found to have the highest content of ascorbic acid with 1000 to 3330 µg of ascorbic acid per 100 g of edible fruit or about 100 times that of oranges and 10 times that of guava or cashew  . Atherosclerotic disease causes over 19 million deaths annually, yet our understanding of the fundamental aspects of the genesis of the disease remains incomplete   . Several studies have shown that an increased dietary intake of cholesterol results in hypercholesterolemia, which is known to eventually generate atherosclerosis and enhance the risk of coronary heart disease (CHD), fatty liver disease and cancer associated with hydroxyl radical formation  . The etiology of atherosclerosis appears to be a multi-factorial series of events, but the oxidation of lipoprotein is believed to be a primary event in the pathogenesis of atherosclerosis  . Thus, recent interest has been focused on strategies to enhance the removal of reactive oxygen species (ROS) by using antioxidants to enhance endogenous antioxidant responses. Natural antioxidants protect dietary lipids from oxidation, but may also provide health benefits associated with preventing damage due to biological degeneration. Antioxidative substances are believed to suppress the onset and development of atherosclerosis and reduce its progression. In addition, flavonoids and phenolic compounds have also been proved to have antioxidative effects. Thus, substances with both antioxidative and hypocholesterolemic properties are expected to be effective in preventing the formation and/or progression of atherosclerosis  . However, relatively less has been studied about the preventive effect of African star apple juice on atherosclerosis. This present study is therefore to evaluate the potentials of orally administered African star apple juice on the development of atherosclerosis by the evaluation of antioxidant enzyme response and lipid profiles in high-cholesterol-fed rats. However, little information about the detailed molecular mechanisms of cardio-protection of African star apple is available.
Based on available information, the detailed molecular mechanism on lipid-lowering effects of African star apple seems quite lacking and warrants further investigation. In addition, a high-fat dietary habit easily promotes production of malondialdehyde (MDA) and reactive oxygen species (ROS) in the body, thus enhancing the occurrence of atherosclerosis. African star apple contains polyphenols which are characterized as having antioxidative and anti-inflammatory capacities  . Hence, serum antioxidant capacity of African star apple juice on a high-fat/cholesterol diet also warrants a further scientific investigation.
The specific objectives of this study were to investigate changes of serum lipid profile in high-fat/cholesterol fed rats supplemented with African star apple juice, and determine oxidative status in high-fat/cholesterol fed rats supplemented with African star apple juice and assess the health implications of a high cholesterol diet.
2. Materials and Methods
2.1. Sample Collection and Preparation
African Star Apple was bought from a local market in Akure, Ondo state, Nigeria. The fruits were thoroughly cleaned by removing all foreign particles and the good ones were sorted out. The fruits were sliced and the seeds discarded while the pulp was scraped and milled using an attrition mill. The African star apple juice was then separated from the milled pulp via a muslin cloth. The juice extracted was then filled into sterilized glass bottles and thereafter, kept at refrigerated temperature for further use.
2.2. Experimental Animals
Twenty-five male Wistar albino rats weighing between 120 g and 150 g were obtained from a private rats weaning outfit near the University of Ibadan, Oyo State, Nigeria. The rats were maintained in accordance with internationally accepted ethical guidelines for the care of laboratory animals. Experimental rats were acclimatized by access to food and water ad libitum for 1 week. They were then randomly assigned to five groups and housed in netted wooden cages at room temperature 25˚C ± 2˚C for an experimental period of twenty-eight days and fed with high cholesterol diet prepared using 1% w/v cholesterol except the control group.
2.3. Experimental Design
Age-matched Albino rats weighing 130 ± 20 grams were divided into five groups (A-E) of five animals per group. The animals were housed into the cage cells.
Group A: Animals in this group received basal diet (normal diet) i.e. Low Fat/Cholesterol Diet throughout the duration of the experiment and served as untreated control.
Group B: Animals consumed High Fat/Cholesterol Diet and water ad libitum throughout the duration of the experiment and served as treated control.
Groups C: Animals consumed High Fat/Cholesterol Diet and water ad libitum and recieved oral admin- stration of 3 ml of African Star Apple Juice.
Group D: Animals in this group consumed High Fat/Cholesterol Diet and water ad libitum and recieved oral adminstration of 6 ml of African Star Apple Juice.
Group E: Animals in this group consumed High Fat/Cholesterol Diet and water ad libitum and recieved oral adminstration of 9 ml of African Star Apple Juice.
2.4. Diet Formulation
The experimental diets were prepared by modifying the standard methods described by  . The compositions of the diets were as shown in Table 1. After a thorough homogenization of the ingredients, the mixture was baked in an oven at a temperature of about 110˚C for 60 min.
2.5. Organ Separation
All animals in the present study were sacrificed using anesthesia. The target organs (heart, liver and kidney) were excised using scissors and forceps.
2.6. Preparation of Serum
Rats were dissected and blood collected through cardiac puncture into sterilizedvials and ejected into clean centrifuge tubes and allowed to stand for one hour. Serum was prepared by centrifugation at 3000× g for 15 minutes at 25˚C. The clear supernatant was used for the serum lipid profile analysis.
Table 1. Composition of experimental diets.
A = Low Fat Cholesterol Diet (LFCD), B = High Fat/Cholesterol Diet (HFCD), C = High Fat/Cholesterol Diet+ 3 ml African Star Apple Juice (HFCD/3), D = High Fat/Cholesterol Diet+ 6 ml African Star Apple Juice (HFCD/6), E = High Fat/Cholesterol Diet+ 9 ml African Star Apple Juice (HFCD/9).
2.7. Preparation of Post-Mitochondrial Fraction (PMF)
The liver tissues were rinsed in ice cold 1.15% potassium chloride solution, blotted with filter paper, and weighed. They were chopped into bits and homogenized in ten volumes of homogenizing buffer (pH 7.4) using a potter-elvegin homogenizer. The resulting homogenate was centrifuged at 10,000× g, 4˚C for 10 minutes to obtain the post mitochondrial fraction. The supernatant was collected, stored under 4˚C and then used for biochemical analysis.
The clear supernatant was used to evaluate the levels of lipid peroxidation (LPO), and reduced glutathione (GSH), and activities of superoxide dismutase (SOD) and catalase (CAT).
2.8. Determination of Phytochemicals in African Star Apple Juice
2.8.1. Test for Flavonoid
Five milliliters (5 ml) of dilute ammonia solution was added to 2 ml of the extract. This was followed by the addition of 2 ml concentrated H2SO4. A yellow coloration was observed and the yellow colouration disappeared on standing  .
2.8.2. Test for Saponin
The ability of saponins to produce frothing in aqueous solution was used as screening test for the compound. Exactly1ml of the extract was shaken with 2 ml distilled water in a test tube, and subsequent sign/manifestation of frothing was carefully observed  .
2.8.3. Test for Tannin
Five milliliters of the extract was stirred with 5 ml distilled water, and the mixture was filtered. The filtrate was treated with ferric chloride, and the prescence of a black-green precipitate showed the presense of tannins  .
2.8.4. Test for Phlobatannin
Five milliliters of the extract was boiled with 5% aqueous HCl. The absence of a red precipitate indicated the absence of phlobatannins  .
2.8.5. Test for Alkaloid
Five grammes of the powdered sample were weighed into 250 ml beaker. Then 100 ml of 10% acetic acid in ethanol was added. The mixture was covered and allowed to stand for 4 h. This was then filtered and the extract concentrated on a water bath to 1/4 of the original volume. Thereafter, concentrated ammonium hydroxide added drop wise until precipitation was completed. The solution was then allowed to settle and the precipitate collected, washed with diluted ammonium hydroxide and filtered. The residue that was dried and weighed was alkaloid  .
2.8.6. Test for Steroid
Two milliliters of acetic acid was added to 0.5 ml of the extract. Two milliliters of concentrated H2SO4 was thereafter added. The prescence of a violet to blue colour indicated the prescence of steroids  .
2.8.7. Test for Terpenoid
Five milliliters of the extract was mixed with 2 ml chloroform. Three milliliters of concentrated H2SO4 was carefully added to the solution to form a thin layer. The absence of a reddish brown coloration at the interface gave a negative result for terpenoids  .
2.8.8. Test for Cardiac Glycoside-Salkowski Test
A small amount of the extract was hydrolysed in 2 ml of HCL solution and neutralized with equal amount of sodiun hydroxide solution. Few drops of fehling’s solution was added. Red precipitates indicated the presence of glycosides  .
2.8.9. Test for Volatile Oil
The extract was dissolved in 90% alcohol and drops of ferric chloride were added. Green colouration, indicate the presence of volatile oils  .
2.9. Determination of Antioxidant Composition
2.9.1. Determination of Vitamin C Content
Vitamin C content of the African star apple juice was determined using the method of Benderitta et al.  . Briefly, 75 µl DNPH (2 g dinitro-phenyl hydrazine, 230 mg thiourea and 270 mg CuSO4∙5H2O in 100 ml of 5 mol∙1−1 H2SO4) was added to 200 µl reaction mixture (300 µl of an appropriate dilution of the polar extract with 100 µl 13.3% (TCA) and water). The reaction mixtures were subsequently incubated for 3 hours at 37˚C, then 0.5 ml of 65% H2SO4 (v/v) was added to the medium, their absorbance was measured at 520 nm and the vitamin C content of the juice was subsequently calculated.
2.9.2. Determination of Total Phenol Content
The total phenol content of the African star apple juice was determined using the method reported by  . Appropriate dilutions of the juice was oxidized with 2.5 ml of 10% Folin-Ciocalteau’s reagent (v/v) and neutralized by 2.0 ml of 7.5% sodium carbonate. The reaction mixture was incubated for 40 minutes at 45˚C and the absorbance was measured at 765 nm in the spectrophotometer. The total phenol content was subsequently calculated using gallic acid as standard.
2.9.3. Determination of Total Flavonoid Content
The total flavonoid content of African star apple juice was determined using a slightly modified method reported by Meda et al.  . Briefly, 0.5 ml of appropriately diluted sample was mixed with 0.5 ml methanol, 50 µl of 10% AICI3, 50 µl of 1 mol∙1−1 potassium acetate and 1.4 ml water and allowed to incubate at room temperature for 30 minutes. Thereafter, the absorbance of the reaction mixture was subsequently measured at 415 nm. The total flavonoid was calculated using quercetin as standard.
2.9.4. DPPH Free Radical Scavenging Ability
The free radical scavenging ability of the African star apple juice against DPPH (1,1-diphenyl-2 picrylhydrazyl) free radical was evaluated as described by Gyamfi et al.  . Briefly, an appropriate dilution of the juice (1 ml) was mixed with 1 ml of 0.4 mmol∙1−1 methanolic solution containing DPPH radicals. The mixture was left in the dark for 30 minutes and the absorbance was measured at 516 nm. The DPPH free radical scavenging ability was subsequently calculated with respect to the reference (which contains all the reagents without the test sample).
2.9.5. Determination of Reducing Property
The reducing property of the African star apple juice was determined by assessing the ability of the extract to reduce ferric chloridesolution as described by Oyaizu  . A 2.5 ml aliquot was mixed with 2.5 ml of 200 mmol∙1−1 sodium phosphate buffer (pH 6.6) and 2.5 ml of 1% potassium ferricyanide. The mixture was incubated at 50˚C for 20 minutes and then 2.5 ml of 10% trichloroacetic acid was added. This mixture was centrifuged at 650× g for 10 minutes. 5 ml of the supernatant was mixed with an equal volume of water and 1 ml of 0.1% ferric chloride. The absorbance was measured at 700 nm. The ferric reducing antioxidant property was subsequently calculated using ascorbic acid as standard.
2.9.6. Fe2+ Chelation Assay
The Fe2+ chelating ability of the African star apple juice was determined using a modified method of Minotti and Aust  with slight modification by  . Freshly prepared 500 µmol∙1−1 FeSO4 (150 µl) was added to a reaction mixture containing 168 µl of 0.1 mol∙1−1 Tris-HCL (pH 7.4), 218 µl saline and the juice (0 - 25 µl). The reaction mixture was incubated for 5 minutes, before the addition of 13 µl of 0.25% 1, 10-phenanthroline (w/v). The absorbance was subsequently measured at 510 nm in a spectrophotometer. The Fe2+ chelating ability was subsequently calculated with respect to the reference (which contains all the reagents without the test sample).
2.10. Serum Lipid Profile Determination
Chemicals: Kit reagents for total cholesterol, triglycerides and high density lipoprotein cholesterol were obtained from a chemical store in Idumota, Lagos State, Nigeria. Dissecting tools and anaesthesia (trichloromethane) were obtained from the Biochemistry Laboratory, Science Technology Department, Federal Polytechnic, Ado-Ekiti, Nigeria.
Sample Collection: The animals were starved overnight for 12 hours before the blood was collected. Rats were anaesthetized with cotton wool treated with trichloromethane (CHCl3) and venous blood samples were collected by direct heart puncture in sterilized vials and ejected into EDTA bottles. The blood samples were centrifuged at 4000 rpm for 10 minutes at 25˚C and serum was recovered.
Serum Lipid Assessment: The levels of serum Total Cholesterol (TC), High Density Lipoprotein-Cholesterol (HDL-C) and Triglycerides (TG) measurement were performed at the Food Chemistry Laboratory, Food Technology Department, Federal Polytechnic, Ado-Ekiti according to GPO-PAD method, CHOD-PAP and Enzymatic end point methods respectively i.e. the manufacturer’s procedures in the kit reagents. Low density Lipoprotein (LDL-C) was calculated according to the methods of  . The atherogenic index (AI) was calculated as.
2.10.1. Determination of Total Cholesterol
Reagent blank and standard were prepared by mixing 10 μl distilled water and 10 μl standard respectively each with 1000 μl of reagent. The three aliquots were separately mixed, incubated for 10 minutes at 25˚C. The absorbance of the samples (Asample) against the reagent blank was measured within 60 minutes at 500 nm-wave- length.
2.10.2. Determination of HDL-Cholesterol
Precipitation: About 500 μl of sample and standard each was separately mixed with precipitant. The mixtures were allowed to sit for 10 minutes at room temperature and then centrifuged for 10 minutes at 4000 rpm. The clear supernatant was separated off within two hours and the cholesterol content was determined by the CHOD- PAP methods i.e. 100 μl each of reagent blank, standard and sample were separately mixed with 1000 μl of reagent. The three aliquots were incubated for 10 minutes at 25˚C. The absorbance of the sample (Asample) and standard (Astandard) were measured against the reagent blank within 60 minutes at 500 nm wavelength.
2.10.3. Determination of Triglycerides
Ten microliter (10 μl) each of standard and sample were separately mixed with 1000 μl of reagent. The mixtures were incubated for 10 minutes at 25˚C. The absorbance of the sample (Asample) and standard (Astandard) were measured against the reagent blank within 60 minutes at 500 nm wavelength
2.10.4. Determination of LDL-Cholesterol
The Low Density Lipoprotein-Cholesterol was calculated according to the method of Friedworld et al.  .
2.10.5. Determination of Atherogenic Index (AI)
The atherogenic index (AI), which is calculated using following formula, is a marker of plasma atherogenicity because it is increased in people with higher risk of cardiovascular diseases. The atherogenic index (AI) was calculated as.
2.11. Biochemical Analysis
2.11.1. Determination of Catalase Activity of Samples
This experiment was carried out using the method described by Sinha  .
One mililiter of sample was mixed with 9 ml distilled H2O to give 1 in 10 dilution of the sample. The assay mixture contained 4 ml of H2O2 solution (800 µmoles) and 5 ml of phosphate buffer in a 10ml flat bottom flask. Properly diluted enzyme preparation (1 ml) was rapidly mixed with the reaction mixture by a gentle swirling motion. The reaction was run at room temperature. A 1 ml portion of the reaction mixture was withdrawn and blown into 2 ml dichromate/acetic acid reagent at 60 seconds intervals. The hydrogen peroxide contents of the withdrawn sample were determined by the method described above.
Catalase activity, K was calculated using the relation below:
where, H2O2 consumed = 800 − H2O2 concentration remaining.
H2O2 concentration remaining is extrapolated from the standard curve for catalase activity.
2.11.2. Determination of Superoxide Dismutase (SOD) Activity
The level of SOD activity was determined by the method of Misra and Fridovich  .
One milliliter of sample was diluted in 9 ml of distilled water to make a 1 in 10 dilution. An aliquot of the diluted sample was added to 2.5 ml of 0.05 M carbonate buffer (pH 10.2) to equilibrate in the spectrophotometer and the reaction started by the addition of 0.3 ml of freshly prepared 0.3 mM adrenaline to the mixture which was quickly mixed by inversion. The reference cuvette contained 2.5 ml buffer, 0.3ml of substrate (adrenaline) and 0.2 ml of water. The increase in absorbance at 480 nm was monitored every 30 seconds for 150 seconds.
where A0 = absorbance after seconds; A3 = absorbance 150 seconds
One unit of SOD activity was given as the amount of SOD necessary to cause 50% inhibition of the oxidation of adrenaline to adrenochrome during 1 minute.
2.11.3. Estimation of Reduced Glutathione (GSH) Level
The method of Bentler  was followed in estimating the level of reduced glutathione (GSH).
The sample, of which 0.2 ml was added to 1.8 ml of distilled water and 3 ml of the precipitating solution was mixed with sample. The rate of addition was not critical. The mixture was then allowed to stand for approximately 5 minutes and then filtered. At the end of the fifth minute, 1 ml of filtrate was added of 4 ml of 0.1 M phosphate buffer. Finally 0.5 ml of the Ellman reagent was added.
A blank was prepared with 4 ml of the 0.1 M phosphate buffer, 1ml of the diluted precipitating solution (3 parts to 2 parts of distilled water) and 0.5 ml of the Ellman reagent. The optical density was measured at 412 nm. GSH was proportional to the absorbance at that wavelength and the estimate was obtained from the GSH standard.
2.11.4. Assessment of Lipid Peroxidation
Lipid peroxidation was determined by measuring the formation of thiobarbituric acid reactive substances (TBARS) according to the method of Vershrey and Kale  .
An aliquot of 0.4 ml of the liver PMF was mixed with 1.6 ml of Tris-KCl buffer to which 0.5 ml of 30% TCA was added. Then 0.5 ml of 0.75% TBA was added and placed in a water bath for 45 minutes at 80˚C. This was then cooled in ice and centrifuged at 3000× g. The clear supernatant was collected and absorbance measured against a reference blank of distilled water at 532 nm. The MDA level was calculated according to the method of Adam and Seregi  . Lipid peroxidation in units/mg protein or gram tissue was computed with a molar extinction coefficient of 1.56 × 105 M−1∙Cm−1.
2.12. Histological Examination of Experimental Animals’ Organs
Histopathological assay of the organs (heart and liver) excised from the rats in the different diet groups (A, B, C, D and E), was determined by reading the photomicrographs obtained from the slides prepared from the organs by a Pathologist at the Veterinary Anatomy Department, University of Ibadan, Nigeria using the standard methods described by Bancroft and Steven  .
Procedure: The tissues pieces were fixed in a suitable fixator, typically formalin and embedded in melted paraffin wax. The wax block was then cut on a microtome to yield a thin slice of paraffin containing the tissue. The specimen slice was then applied to a microscope slide, air dried and heated to cause the specimen to adhere to the glass slide. Residual paraffin was then dissolved and rinsed with an acid-alcohol followed by rinsing with water to remove the acid alcohol. The hematoxylin was blued by the bluing solution. The bluing solution was removed by rinsing with water. Other cytoplasmic elements were stained with an alcoholic solution of eosin Y, a red stain and a light green. The excess stain was removed by water in series of sequential washes in a dehydrating reagent. The slide was contacted with a chemical clearing agent (toluene, xylene or t-butanol) to remove residual dehydrating reagent remaining from the washing step. A cover slip mountant was applied to the slide after removing the slide from a chemical clearing agent. The clearing agent evaporates and the mountant hardens leaving a stained and mounted slide. Histopathological assessment and photomicrography of the prepared slides was done by a pathologist using an Olympus light microscope with attached Kodak digital camera.
2.13. Statistical Analysis
All analyses were carried out in triplicate and data were subjected to Analysis of Variance (ANOVA) using Statistical Package for Social Sciences (SPSS) version 15.0 (for windows). Statistical differences between mean values were determined by Duncan’s Multiple Range Tests and accepted at P < 0.05.
3. Results and Discussion
3.1. Phytochemical Screening
Table 1 shows the results of the phytochemical screening of Chrysophyllum albidum juice. The phytochemical screening of Chrysophyllum albidum juice revealed the presence of steroids, tannins, alkaloids, flavonoids, cardiac glucoside, saponins and volatile oil while there was absence of phlobatannins and terpenoids.
3.2. Antioxidant Indices
The results for the evaluation of antioxidant properties of African Star Apple juice as assessed by sixin vitro methods (Vitamin C, Flavonoid, Phenol, DPPH, ferric reducing antioxidant property and Iron (II) chelating ability)are shown in Table 2.
Table 2. Antioxidant indices of Chrysophyllum albidum.
Values in the table are expressed as mean ± SEM (n = 5).
3.3. Serum Lipid Profile of Albino Rats after 4-Week Consumption of Experimental C. albidum Diets
The results of the serum lipid profile (in vivo) are shown in Table 3. The values obtained for each of the parameters did not exceed the safe limits of US guidelines for serum lipid profile. Highest blood cholesterol level was recorded for the animals that fed on High Fat/Cholesterol diets. Since the elevation of serum cholesterol, triglycerides and β-lipoprotein are of health significance, HFCD/9 would be better for consumption.
3.4. Biochemical Analysis
The results of the biochemical analysis are shown in Figures 1-4. The liver was assayed for Catalase, Superoxide Dismutase, Reduced Glutathione and Lipid Peroxidation. The result obtained showed that ASAJ inhibited
Table 3. Serum lipid profile of albino rats after 4 week consumption of experimental C. albidum diets (mg/dl).
Values in the table are expressed as mean ± SD for five Animals. Means in the same row with different superscripts are significantly different (P < 0.05).
Figure 1. Effect of Chrysophyllum albidum on liver catalase level of rats fed High fat/Cholesterol diet.
Figure 2. Effect of Chrysophyllum albidum on liver superoxide dismutase level of rats fed high fat/Cholesterol diet.
Figure 3. Effect of Chrysophyllum albidum on liver glu- tathione level of rats fed High fat/Cholesterol diet.
Figure 4. Quantification of extent of lipid peroxidation in winstar rat livers.
HFCD decrease in catalase, superoxide Dismutase and Reduced Gluthatione activities and HFCD increase in MDA level.
3.5. Organs Histology
The results of histological examination of the organs excised from the albino rats that fed on experimental C. albidum diets over a four-week period are shown in Figure 5 and Figure 6. The summary of the conclusions drawn from the photomicrographs are shown in Table 4. The photomicrographs were magnified (×400) to show the conditions of the cell vacuoles and hepatocytes. Low Fat/Cholesterol diets gave the safest results.
The results in Table 5 show the phytochemical analysis of the C. albidum juice was found to contain saponins, alkaloids, cardiac glycosides ,tannins, flavonoids, steroids and volatile oil, while it showed absence of phlobatannins and terpenoids.
African star apple juice showed the presence of steroids, tannins, alkaloids, flavonoids, cardiac glycoside,
(a) (b)(c) (d)(e)
Figure 5. Histological Examination of Heart (photomicrographs) (×400 mag.) (a) = LFCD, (b) = HFCD, (c) = HFCD/3, (d) = HFCD/6, (e) = HFCD/9.
saponins and volatile oil. The presence of these phytochemicals in African star apple juice is suggestive of its potent bioactivity. Specifically, saponin is known to elicit serum cholesterol lowering activity by causing resin- like action, thereby reducing the enterohepatic circulation of bile acids  . Presence of tannins suggests the ability of this plant to play a major role as anti-diarrhoeic and anti-haemorrhagic agent  . The cardiac glycosides have been used for over two centuries as stimulants in the case of cardiac failure  .
Plants are enriched with bioactive phytochemicals that might be effective therapy, safe and cheap. The phytochemical analysis of Chrysophylum albidum juice showed the presence of secondary metabolite such as
(a) (b)(c) (d)(e)
Figure 6. Histological Examination of Liver (photomicrographs) (×400 mag.) (a) = LFCD, (b) = HFCD, (c) = HFCD/3, (d) = HFCD/6, (e) = HFCD/9.
alkaloids, saponins, steroids, cardiac glycosides, tannins, flavonoids and volatile oil, while phlobatannins and terpenoids were not found. Studies have shown that the polyphenolic content in the plants are associated with their antioxidant activities and good potentials as anti-inflammatory, anti-diarrheal and anti-hemorrhoidal compound  . Hence, the presence of cardiac glycoside in Chrysophylum albidum makes it to be effective in congestive heart failure as reported by Aboaba et al.  . The presences of these metabolites confirm the usefulness of the plant in the treatment of various diseases  . The plant exhibited potent antioxidant activity. The presence of phenolic compound like tannins could be responsible for the free radical scavenging effect.
3.7. Anitoxidant Activity of African Star Apple Juice
The evaluation of phenol content and flavonoid content showed that African star apple juice is considerably rich
Table 4. Summary of histopathology assay of organs from albino rats after 4 week consumption of African star apple juice experimental diets.
LFCD = Low fat/cholesterol diet; HFCD = High fat/cholesterol diet; HFCD/3 = High fat/cholesterol diet + 3 ml African star apple juice; HFCD/6 = High fat/cholesterol diet + 6 ml African star apple juice; HFCD/9 = High fat/cholesterol diet + 9 ml African star apple juice; L = Liver; H = Heart muscle.
Table 5. Phytochemical screening of the Juice of the fruit of Chrysophyllum albidum.
(+) Present; (−) absent.
in phenol and flavonoids. Studies have shown phenol rich diets reduce oxidative DNA damage, Oxidative stress  . Hao et al.  found that Phenolic compounds are good electron donors and could terminate the radical chain reaction by converting free radical to more stable products.
The scavenging effect of phenolics (flavonoids) such as Quercetin has also been observed to significantly decrease water induced Reactive Oxygen Species elevation and has been recorded to inhibit oxidation modification of Low Density Lipoprotein and cytotoxicity of low density lipoprotein  . Flavonoids also inhibit cyclooxygenase, leading to lower platelet aggregation and reduced thrombotic tendencies  .
African star apple juice showed a considerable content of total flavonoids which may possibly be responsible for the antioxidant activity coupled with the fact that phenolic and flavonoid compounds have been reported by Carew et al.  to be effective antioxidant constituents in plant foods including fruits, vegetables and grains  . These Cardio-protective actions of flavonoids, especially quercetin can be hypothesized to be manifested by African star apple juice.
The hydroxyl radical is an extremely reactive free radical formed in the biological systems and has been implicated as a highly damaging species in free radical pathology, capable of damaging almost every molecule found in living cells  . Scavenging of hydroxyl radicals is likewise an important antioxidant activity because of very high reactivity of the DPPH and OH- radicals which reduces its ability to react with a wide range of molecules found in living cells, such as amino acids, sugars, lipids and nucleotides  .
The DPPH assay of African star apple juice validated its free radical scavenging activity. The scavenging ability was found to be concentration dependent.
3.8. Serum Lipid Profile of Blood Obtained from Albino Rats after 4-Week Consumption of Experimental African Star Apple Juice Supplemented Diets
The total blood cholesterol (TC), Triglycerides (TG), High Density lipoprotein Cholesterol (HDL-C) and Low Density Lipoprotein Cholesterol (LDL-C) values ranged from 109.81 - 215.67, 91.45 - 187.48, 20.10 - 46.40, and 45.12 - 158.07 mg/dl respectively. The values conform and are within the <240 mg/dl (TC), <200 mg/dl (TG), ≥10 mg/dl (HDL-C) and < 160 mg/dl (LDL-C) recommended for healthy individuals by the US guideline on health (mnuqol.com/blood-lipid-profile.htm); as such, none of the experimental animals have the tendency to develop hypercholesteromia and other serum-lipid induced/related health issues.
The oral administration of 1% cholesterol in High fat/cholesterol diet increased plasma total cholesterol, triglycerides, LDL, and VLDL to 215.67, 187.48, 158.07, and 37.65 mg/dl respectively, as compared to the normal control. These significant rises were accompanied by a significant decrease in plasma HDL cholesterol to 20.10 mg/dl, as compared to the normal control (Table 3). The atherogenic index compared to the normal control was also increased significantly to 7.61 (Table 3). The oral supplementation of the extract of African star apple juice (3 ml, 6 ml, and 9 ml) to high cholesterol rats resulted in significant declines in plasma total cholesterol level to 182.77, 165.90 and 169.74 mg/dl respectively as compared to the high cholesterol fed rats (215.67 mg/dl). LDL, VLDL, and triglycerides also showed a significant decrease as compared to the high cholesterol fed rats to 117.21, 29.72, and 148.32 for 3 ml African star apple juice extract, 113.87, 25.76, and 128.15 for 6 ml African star apple juice extract and 105.85, 24.27 and 122.47 for 9 ml African star apple juice (Table 3). Similarly atherogenic index was also reduced to about 4.09, 5.28 and 3.31 for African star apple juice extract (3 ml, 6 ml, 9 ml) respectively (Table 3). It also elevated plasma HDL cholesterol to 35.9, 26.40 and 39.4 respectively.
In the human body, high levels of triglycerides in the bloodstream have been linked to atherosclerosis, and, by extension, the risk of heart disease and stroke. TG level was significantly decreased in the experimental groups (HFCD/3, HFCD/6, HFCD/9) compared to the treated control group (HFCD) (P > 0.05). High cholesterol levels are strong indicators of those individuals that are prone to coronary heart disease. Elevated total cholesterol is a risk factor for coronary heart disease. The build-up of plaque in the artery may lead to narrowing (high blood pressure) or complete blockage (heart attack) of the vessel  . It is widely accepted that reduction in plasma HDL-C is a risk factor for developing atherosclerosis. HDL-C facilitates the translocation of cholesterol from the peripheral tissue, such as arterial walls to liver for catabolism. The increase in HDL-C may slow down the atherosclerotic process  . The results showed that the untreated control (LFCD) and African star apple juice supplemented-diets (HFCD/3, HFCD/6, HFCD/9) increased the concentrations of serum HDL-C when is compared with the cholesterol-rich diet (HFCD), and the concentrations of serum LDL-C too, were decreased.
Yeo and Su  reported that a statistically significant association occurs between elevated serum Total cholesterol and Low Density Lipoprotein Cholesterol (LDL-C) and the severity of Retinal hard exudation in patients with diabetic retinopathy. In the same study,  the researchers reported a literature support that oedema and hemorrhage may result from the incorporation of triglycerides into the cell membrane leading to changes in membrane fluidity and leakage of plasma constituents into the retina. Elevated levels of Triglyceride, Cholesterol and LDL-C are documented as risk factors for atherogenesis. LDL-C in its oxidized or acetylated form has been identified as a major atherogenic particle; as it not only load macrophages with cholesterol for the formation of foam cells but also because it is chemotactic for circulating monocytes, is cytotoxic and can adversely alter coagulation pathways  . The blood level of HDL-C in contrast bears an inverse relationship of the risk of atherosclerosis and coronary heart disease that is, higher the level, smaller the risk   . Literature shows that correlations exist between Serum lipid profiles and sperm parameters. Vigrion et al.  found that increased triglyceride has deleterious effects on spermatogenesis. In like findings, Ergun et al.  investigated the correlations of semen parameters with lipid concentrations among 18 infertile men. Their results showed that increase of very Low Density Lipoprotein (LDL-C) and Triglyceride were significantly correlated with decreased sperm motion characteristics. Mohammad et al.  found out that triglyceride above normal level was related with abnormal sperm morphology and motility.
3.9. Biochemical Analysis of Organs from Albino Rats after 4-Week Consumption of African Star Apple Juice Supplimented Diets
The best cellular antioxidants are the enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH). Antioxidant enzymes such as SOD, reduced glutathione (GSH), catalase (CAT), play a vital role in protecting the cells from oxidative damage and are usually depleted during oxidative stress condition  . The scavenging activities observed against DPPH and hydroxyl radicals, as well as protective activities against lipid peroxidation, lead to proposing African star apple juice extract as a promising natural source of antioxidant suitable for application in nutritional/pharmacological fields, and in the prevention of free radical-mediated diseases.
Superoxide dismutase, (SOD) is a specific antioxidant enzyme which dismutates and forms H2O2 that is eventually scavenged by catalase or glutathione peroxidase   . These enzymes prevent generation of hydroxyl radical and protect the cellular constituents from oxidative damage  . The significant decrease (P ˂ 0.05) in activities of SOD in High Fat/Cholesterol Diet (HFCD) treated rats might be due to increase production of the superoxide anion radical and the channeling of the antioxidant enzymes towards its removal  . Hence, the observed inhibition of HFCD effect in African star apple juice (3, 6, and 9 ml) treated rats Figure 2 suggested that African star apple juice at those dosage were capable of either attenuating HFCD-induced superoxide anion production or boosting cardiac cells SOD production capacity.
Catalase, the perixosomal antioxidant enzyme, is frequently used by cells to rapidly catalyze the decomposition of hydrogen peroxide into less reactive gaseous oxygen and water molecules  . Hydrogen peroxide is a harmful by-product of many normal metabolic processes, and to prevent damage, it must be quickly converted into other, less dangerous substances. The significant decrease in catalase activity of HFCD-treated rats lends support to the claim that H2O2 is one of the products of HFCD metabolism  . African star apple juice (3, 6, and 9 ml) treatment therefore proved protective by reversing the HFCD decrease in the antioxidant enzyme level.
The endogenous antioxidant molecule, glutathione (GSH), can interact directly with certain ROS (like hydroxyl radical) to detoxify them, as well as performing other critical activities in the cell  . The level of GSH, which comprises in most instances the bulk of cellular non-protein sulfhydryl groups, is a measure of cellular redox status  , and is usually depleted during oxidative stress conditions  .
GSH is also an essential component of the glutathione peroxidase system where the selenium-containing microsomal enzyme, GPx, catalyses the degradation of hydrogen peroxide to water and reduces organic peroxides to alcohols, providing another route for eliminating toxic oxidants.
The observed depletion in GSH levels in HFCD-treated rat is in line with previous reports  , further confirming the lipid peroxidation caused by HFCD. Hence the observed inhibition of HFCD depletion in African star apple juice lends support to the protective antioxidant benefit of the intervention.
Malonaldehyde (MDA) is one of the degradative products of free radical-mediated oxidation of polyunsaturated fatty acids (PUFA) in biological membranes  . The production of this aldehyde is used as a biomarker to measure the level of oxidative stress in an organism since lipid peroxidation is directly related to oxidative damage  . The H2O2 resulting from African star apple juice metabolism can be converted to highly toxic hydroxyl radicals (OH−) in the presence of transition metal, most often Fe2+  . These ROS are toxic and react with lipids, proteins and nucleic acids, resulting in lipid peroxidation, depletion of sulfhydryl-containing peptides (glutathione inclusive), and damage to DNA. Hence, the observed inhibition of HFCD MDA elevation in ASAJ supported the protective antioxidant benefit of ASAJ.
3.10. Histological Assay of Organs from Albino Rats after 4-Week Consumption of African Star Apple Juice Treated Experimental High Fat/Cholesterol Diets
The histological assays of hearts and livers from albino rats after 28 days consumption of experimental High fat/cholesterol diet are shown in Figure 5. The figure showed that there were no inflammatory or degenerative conditions seen in the histological structure of organs (heart and liver) excised from the animals that fed on low fat/cholesterol diet. This may be due to the fact that the diet did not contain toxic polycyclic aromatic hydrocarbons (PAHs) and dioxin (Polychlorinated dibenzo-p-dioxin and polychlorodibenzo furans) a family of polychlorinated tricyclic aromatic compounds which are documented for their mutagenic or carcinogenic potential  . The result obtained during the heart histology also showed that the fat in the diet are not thermal stressed and so cytotoxic aldehyde product which could promote the induction, development and progression of CVD are not generated  .
From the summary of the histopathology of the heart and liver obtained from the animals that fed on High fat/cholesterol diet, the heart maintained normal cardiac muscle bundle and normal cardiac myocytes but with thickened wall vascular channel which is an evidence of severe degeneration of vascular wall. In the liver, there is a very severe degeneration of hepatocytes and hepatic necrosis (cell death). The occurrence of hepatocytes necrosis as induced by the consumption of High Fat/Cholesterol Diet is evidence of hepatitis  . This evidence showed that the High Fat/Cholesterol Diet possessed some chemical and pharmacological properties similar to the classes of drugs that are capable of inducing liver damage and this explains their capability to effect the histological changes observed.
Figure 5 and Figure 6 show the histology of the heart and liver obtained from 3 ml ASAJ treated animals that fed on High Fat/Cholesterol Diet. This histology showed the heart does not have any visible lesion. There were no inflammatory or degenerative conditions seen in the heart. This may be attributed to lipid lowering effects of ASAJ. Also ASAJ contains polyphenols which are characterized as having antioxidative and anti-inflammatory capacities. The liver showed normal portal triad but slightly distended sinusoid. This showed that the location for the oxygen rich blood from the hepatic artery and the nutrient rich blood from the portal vein has been affected  . The distended sinusoid seen in the liver of 3 ml ASAJ treated animals fed on High Fat/Cholesterol Diet may be due to dosage dependence.
The histopathology of the heart and liver of the 6 ml ASAJ treated animals fed on High Fat/Cholesterol Diet are clearly designated. The histology showed that the heart does not have any visible lesion. ASAJ treatment therefore had a protective effect against myocardial infarction. The liver showed degeneration of hepatocytes with mild periportal inflammation of the portal triad. The protective capacity of the ASAJ against liver degeneration could be attributed to dosage dependence.
Figure 5 and Figure 6 also show the histology of the heart and liver of 9 ml ASAJ treated animals fed with High Fat/Cholesterol Diet. Both the heart and the liver obtained did not show any visible lesion and there were no inflammatory or degenerative conditions seen in these organs. This may be attributed to serum antioxidant capacity of African Star Apple juice on a High Fat/Cholesterol Diet. This study has aligned with previous reports   that the solution to most human nutritional and health problems lies in the exploitation of underutilized and lesser-known wild forest seeds.
The present study demonstrated that diet-induced hypocholesterolemic atherosclerosis was associated with an increase in the oxidative stress and that African star apple juice reduced the extent of atherosclerosis by reducing oxidative stress and serum TC, TG, LDL-C and raising serum HDL-C. Also, treatment with the African star apple juice offered protection against high fat/cholesterol diet-induced damage to the cardiac tissues possibly through positive modulation of the cardiac antioxidant system. African star apple juice is a promising supplement for improving defence mechanisms in the body system against oxidative stress. The findings therefore support the ethno medicinal uses of the juice in the management of cardiovascular complications like atherosclerosis and hypertension.
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