AiM  Vol.6 No.9 , August 2016
Characterization of a Novel MDH1 Bacterium from a Virgin Hot Spring Applicable for Gold Nanoparticle (GNPs) Synthesis
Abstract: Background: Use of novel microorganisms for beneficial purposes is still remaining a challenging job. This study was designed to isolate, characterize and use of a novel hot spring bacterial strain from a virgin hot spring of Metaldanga, Birbhum, West Bengal, India. Methods: A pure bacterial strain (MDH1) was identified by growing the enrichment culture isolated from Metaldanga hot spring through serial dilution process in a semi-synthetic medium at pH 8.0 and 42°C temperature. The novelty of the strain was characterized by 16S-rRNA gene sequence analysis. The bacterium acted as template to synthesize spherical gold nanoparticles (GNPs). GNPs were characterized by using UV-Vis spectroscopy, X-ray diffraction (XRD), dynamic light scattering (DLS) and Fourier transform infra-red spectroscopy (FTIR). Results: The phylogenetic analysis suggested that MDH1 strain (GenBank accession number: KT600031) was affiliated to the family “Pseudomonadaceae” with 99% homologous to Pseudomonas putida H8234. The coccoid shaped bacterium was gram-negative and facultative-anaerobic which acted as a template to synthesize spherical GNPs with an average size of 12 ± 3 nm when examined under transmission electron microscopy (TEM). FT-IR studies revealed the presence of bioactive functional groups which acted as capping and stabilizing agents of the GNPs. XRD pattern confirmed the amorphous nature of GNPs. The Zeta potential (ζ) concluded the adequate stability of GNPs in an aqueous environment. Conclusions: The present investigation explores the microbial diversity of a virgin hot spring of Metaldanga for its beneficial applications in industry, particularly in the synthesis of the gold nanoparticles.
Cite this paper: Alam, N. , Sarkar, M. , Chowdhury, T. , Ghosh, D. and Chattopadhyay, B. (2016) Characterization of a Novel MDH1 Bacterium from a Virgin Hot Spring Applicable for Gold Nanoparticle (GNPs) Synthesis. Advances in Microbiology, 6, 724-732. doi: 10.4236/aim.2016.69071.

[1]   Tang, K.H., Tang, Y.J. and Blankenship, R.E. (2011) Carbon Metabolic Pathways in Phototrophic Bacteria and Their Broader Evolutionary Implications. Frontier in Microbiology, 2, 165.

[2]   Ilyas, S., Anwar, M.A., Niazi, S.B. and Ghauri, M.A. (2007) Bioleaching of Metals from Electronic Scrap by Moderately Thermophilic Acidophilic Bacteria. Hydrometallurgy, 88, 180-188.

[3]   Ghosh, S.K. and Pal, T. (2007) Interparticle Coupling Effect on the Surface Plasmon Resonance of Gold Nanoparticles: From Theory to Applications. Chemical Review, 107, 4797-4862.

[4]   Patra, J.K. and Baek, K.H. (2015) Novel Green Synthesis of Gold Nanoparticles Using Citrullus lanatus Rind and Investigation of Proteasome Inhibitory Activity, Antibacterial, and Antioxidant Potential. International Journal of Nanomedicine, 10, 7253-7264.

[5]   Narayanan, K.B. and Sakthivel, N. (2010) Phytosynthesis of Gold Nanoparticles Using Leaf Extract of Coleus amboinicus Lour. Material Characterization, 61, 1232-1238.

[6]   Thakkar, K.N., Mhatre, S.S. and Parikh, R.Y. (2010) Biological Synthesis of Metallic Nanoparticles. Nanomedicine Nanotechnology Biology and Medicine, 6, 257-262.

[7]   Xu, C., Wang, X. and Zhu, J. (2008) Graphene-Metal Particle Nanocomposites. The Journal of Physical Chemistry C, 112, 19841-19845.

[8]   Goncalves, G., Cruz, S.M., Ramalho, A. and Gracio, J. (2012) Graphene Oxide versus Functionalized Carbon Nanotubes as a Reinforcing Agent in a PMMA/HA Bone Cement. Nanoscale, 4, 2937-2945.

[9]   Huang, D., Niu, C., Ruan, M., Wang, X., Zeng, G. and Deng, C. (2013) Highly Sensitive Strategy for Hg2+ Detection in Environmental Water Samples Using Long Lifetime Fluorescence Quantum Dots and Gold Nanoparticles. Environmental Science and Technology, 47, 4392-4398.

[10]   Sarkar, M., Adak, D., Tamang, A., Chattopadhyay, B.D. and Mandal, S. (2015) Genetically-Enriched Microbe-Facilitated Self-Healing Concrete—A Sustainable Material for a New Generation of Construction Technology. RSC Advances, 5, 105363-105371.

[11]   Sarkar, M., Alam, N., Chaudhuri, B., Chattopadhyay, B.D. and Mandal, S. (2015) Development of an Improved E. coli Bacterial Strain for Green and Sustainable Concrete Technology. RSC Advances, 5, 32175-32182.

[12]   Chattopadhyay, B.D., Thakur, A.R., et al. (1994) Inhibitory Role of Aluminium in Methane Emission in Rice Field. Indian Journal of Experimental Biology, 32, 495-500.

[13]   Wilson, K. (1987) Preparation of Genomic DNA from Bacteria. In: Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. and Struhl, K., Eds., Current Protocols in Molecular Biology, Wiley & Sons, New York, 2.4.1-2.4.5.

[14]   Tamura, K., Peterson, D., Stecher, N. and Nei, G. (2011) MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731-2739.

[15]   Alfaro, M.E., Zoller, S. and Lutzoni, F. (2003) Bayes or Bootstrap? A Simulation Study Comparing the Performance of Bayesian Markov Chain Monte Carlo Sampling and Bootstrapping in Assessing Phylogenetic Confidence. Molecular Biology and Evolution, 20, 255-266.

[16]   Chattopadhyay, B.D., Alam, N., Sarkar, M. and Chowdhury, T. (2016) Pseudomonas Putida Strain MDH1 16S Ribosomal RNA Gene, Partial Sequence. GenBank.

[17]   Chauhan, A., Zubair, S., Tufail, S., Sherwani, A., Sajid, M., Raman, S.C, Azam, A. and Owais, M. (2011) Fungus-Mediated Biological Synthesis of Gold Nanoparticles: Potential in Detection of Liver Cancer. International Journal of Nanomedicine, 6, 2305-2319.

[18]   Chaudhuri, B., Alam, N., Sarkar, M., Chowdhury, T. and Chattopadhyay, B.D. (2016) Phylogenetic Characterization of BKH3 Bacterium Isolated from a Hot Spring Consortium of Bakreshwar (India) and Its Application. Advances in Microbiology, 6, 453-461.

[19]   Biswas, M., Majumdar, S., Chowdhury, T., Chattopadhyay, B.D., Mandal, S., Halder, U. and Yamashaki, S. (2010) Bioremediase a Unique Protein from a Novel Bacterium BKH1, Ushering a New Hope in Concrete Technology. Enzyme and Microbial Technology, 46, 581-587.

[20]   Chowdhury, T., Sarkar, M., Chaudhuri, B., Chattopadhyay, B.D. and Halder, U. (2015) Participatory Role of Zinc in Structural and Functional Characterization of Bioremediase: A Unique Thermostable Microbial Silica Leaching Protein. Journal of Biological Inorganic Chemistry, 20, 791-803.