OALibJ  Vol.2 No.12 , December 2015
Effects of the Dietary Supplement MAK4 on Oxidative Stress Parameters: A “Three-Cases” Report
Abstract: Background: Maharishi Amrit Kalash-4 (MAK-4) is an herbal formulation comprised of several herbs. This preparation is part of a natural health care system known as Maharishi Ayur-Veda and belongs to a series of herbal mixtures collectively known as Rasayanas, which are recognized for their beneficial effect on human health. This investigation evaluated in blood samples of 3 human volunteers, the effects of MAK-4 on parameters of oxidative stress and antioxidant defenses. Methods: The day of the experiment: 3 subjects were put on the following diets: Diet 1: 300 gr boiled vegetables (zucchine and potatoes) and 100 gr of wheat bread (control); Diet 2: 300 gr raw vegetables (lattuge, carrots, cavoliflowers) and 100 gr of wheat bread; Diet 3: diet 1 plus 500 gr of oranges; Diet 4: diet 1 plus MAK-4 (30 gr/day). Short term treatments: The same subjects have been on the four different diets on alternative days. Meals were at 12 am and 6:30 pm. MAK-4 (10 gr each intake) was assumed three times a day at 9 am, 12 am and 6:30 pm (total 30 gr). Blood was withdrawn three times a day, at 9 am (T = 0), just before the first intake of MAK-4 (first), at 7 pm (second) and at 9 am, the following day (third). Long term treatments: subjects were on a free personal diet for 60 days (control). For the following 60 days the subjects were on the same diet plus 20 gr of MAK-4/day. MAK-4 (10 gr) was assumed twice a day, at 9 am and at 6 pm for 60 days. Blood was withdrawn at the beginning of the study (T = 0) and after 60 days, at the same day time (9 am). Results: In the short term study MAK-4 increased two endogenous parameters of antioxidant defenses: Oxygen radical absorbance capacity (ORAC) and total thiols. Peak induction was observed at 9 hours after the assumption of the first intake. Up-regulation of ORAC was similar to that obtained with 500 grs of oranges although with MAK-4 the ORCA value remained sensibly higher than the control at 24 hours. Thiols maximally increased with MAK-4 at 9 hours, but returned to basal levels at 24 hours. Exogenous intake of ascorbic acid contained in MAK-4 showed a maximal level at 9 hours, although generally lower than that assumed with 500 gr of oranges. At 24 the ascorbic acid from MAK-4 was at the same level of that assumed with oranges. The same parameters measured in blood of the subject on Diet 2 showed a slight increase at 9 hours, although it was significant only for ascorbic acid. In the long term study the MAK-4-supplemented diet caused a decrease in a parameter of oxidative load (hydroperoxides) and an upregulation of ORAC, thiols and ascorbic acid. The same subjects did not show the same variations when they were on the basal diet alone. Conclusions: Our results show that a MAK-supplemented diet decreases oxidative stress parameters and increases antioxidant defenses in both short and long term treatments.
Cite this paper: Zanella, I. , Lorenzo, R. and Lorenzo, D. (2015) Effects of the Dietary Supplement MAK4 on Oxidative Stress Parameters: A “Three-Cases” Report. Open Access Library Journal, 2, 1-8. doi: 10.4236/oalib.1102150.

[1]   Sharma, H. and Clark, C. (1998) Contemporary Ayurveda: Medicine and Research in Maharishi Ayur-Veda. Churchill Livingstone Press, London.

[2]   Sharma, H. (1993) Freedom from Disease: How to Control Free Radicals, a Major Cause of Aging and Disease. Veda Publishing, Toronto.

[3]   Penza, M., Montani, C., Jeremic, M., Mazzoleni, G., Hsiao, W.L., Marra, M., Sharma, H. and Di Lorenzo, D. (2007) MAK-4 and -5 Supplemented Diet Inhibits Liver Carcinogenesis in Mice. BMC Complementary and Alternative Medicine, 8, 7-19.

[4]   Sharma, H.M. and Alexander, C.N. (1996) Maharishi Ayurveda: Research Review. Part 2: Maharishi Ayurveda Herbal Food Supplements and Additional Strategies. Complementary Medicine International, 3, 17-28.

[5]   Gaddipati, J.P., Rajeshkumar, N.V., Thangapazham, R.L., Sharma, A., Warren, J., Mog, S.R., Singh, A.K. and Maheshwari, R.K. (2004) Protective Effect of a Polyherbal Preparation, Brahma Rasayana against Tumor Growth and Lung Metastasis in Rat Prostate Model System. Journal of Experimental Therapeutics and Oncology, 4, 203-212.

[6]   Inaba, R., Sugiura, H. and Iwata, H. (1995) Immunomodulatory Effects of Maharishi Amrit Kalash 4 and 5 in Mice. Japan Journal of Hygiene, 50, 901-905.

[7]   Inaba, R., Sugiura, H., Iwata, H., Mori, H. and Tanaka, T. (1996) Immunomodulation by Maharishi Amrit Kalash 4 in Mice. Journal of Applyed Nutrition, 48, 10-21.

[8]   Sharma, H.M., Dwivedi, C., Satter, B.C., Gudehithlu, K.P., Abou-Issa, H. and Malarkey, W. (1990) Antineoplastic Properties of Maharishi-4 against DMBA-Induced Mammary Tumors in Rats. Pharmacology, Biochemistry and Behavior, 35, 767-773.

[9]   Sharma, H.M., Dwivedi, C., Satter, B.C. and Abou-Issa, H. (1991) Antineoplastic Properties of Maharishi Amrit Kalash, an Ayurvedic Food Supplement, against 7,12-dimethylbenz(a)anthracene-Induced Mammary Tumors in Rats. Journal of Research and Education in Indian Medicine, 10, 1-8.

[10]   Patel, V.K., Wang, J., Shen, R.N., Sharma, H.M. and Brahmi, Z. (1992) Reduction of metastases of Lewis Lung Carcinoma by an Ayurvedic Food Supplement in Mice. Nutrition Research, 12, 51-61.

[11]   Prasad, K.N., Edwards-Prasad, J., Kentroti, S., Brodie, C. and Vernadakis, A. (1992) Ayurvedic (Science of Life) Agents Induce Differentiation in Murine Neuroblastoma Cells in Culture. Neuropharmacology, 31, 599-607.

[12]   Rodella, L., Borsani, E., Rezzani, R., Lanzi, R., Lonati, C. and Bianchi, R. (2004) MAK-5 Treatment Enhances the Nerve Growth Factor-Mediated Neurite Outgrowth in PC12 Cells. Journal of Ethnopharmacology, 93, 161-166.

[13]   Arnold, J.T., Wilkinson, B.P., Korytynski, E.A. and Steel, V.E. (1991) Chemopreventive Activity of Maharishi Amrit Kalash and Related Agents in Rat Tracheal Epithelial and Human Tumor Cells. Proceedings of the American Association for Cancer Research, 32, 128-131.

[14]   Yamasaki, H. and Naus, C. (1995) Role of Connexin Genes in Growth Control. Carcinogenesis, 17, 1199-1213.

[15]   Wells, P.G., McCallum, G.P., Chen, C.S., Henderson, J.T., Lee, C.J., Perstin, J., Preston, T.J., Wiley, M.J. and Wong, A.W. (2009) Oxidative Stress in Developmental Origins of Disease: Teratogenesis, Neurodevelopmental Deficits and Cancer. Toxicological Sciences, 108, 4-18.

[16]   Stathopoulos, G.P., Malonos, N.A., Dontas, I., Deliconstantinos, G., Perrea-Kotsareli, D. and Karayannacos, P.E. (1998) Inhibition of Adriamycin Cardiotoxicology by 5-Fluorouracil: A Potential Free Oxygen Radical Scavenger. Anticancer Research, 18, 4387-4392.

[17]   Maynard, S., Schurman, S., Harboe, C., de Souza-Pinto, N.C. and Bohr, V.A. (2009) Base Excision Repair of Oxidative DNA Damage and Association with Cancer and Aging. Carcinogenesis, 30, 2-10.

[18]   Badjatia, N., Satyam, A., Singh, P., Seth, A. and Sharma, A. (2010) Altered Antioxidant Status and Lipid Peroxidation in Indian Patients with Urothelial Bladder Carcinoma. Urologic Oncology: Seminars and Original Investigations, 28, 360-367.

[19]   Khansari, N., Shakiba, Y. and Mahmoudi, M. (2009) Chronic Inflammation and Oxidative Stress as a Major Cause of Age-Related Diseases and Cancer. Recent Patents on Inflammation & Allergy Drug Discovery, 3, 73-80.

[20]   Sharma, H., Zhang, X. and Dwivedi, C. (2010) The Effect of Ghee (Clarified Butter) on Serum Lipid Levels and Microsomal Lipid Peroxidation. Ayu, 31, 134-140.

[21]   Hanna, A.N., Sundaram, V., Falko, J.M., Stephens, R.E. and Sharma, H.M. (1996) Effect of Herbal Mixtures MAK-4 and MAK-5 on Susceptibility of Human LDL to Oxidation. Complementary Medicine International, 3, 28-36.

[22]   Tomlinson, P.F. and Wallace, R.K (1996) Superoxide Scavenging of Two Natural Products, Maharishi-4 (M-4) and Maharishi-5 (M-5). The FASEB Journal, 5, A1284.

[23]   Sharma, H.M., Hanna, A.N., Kauffman, E.M. and Newman, H.A.I. (1992) Inhibition of Human LDL Oxidation in Vitro by Maharishi Ayur-Veda Herbal Mixtures. Pharmacology Biochemistry and Behavior, 43, 1175-1182.

[24]   Lee, J.Y., Hanna, A.N., Lott, J.A. and Sharma, H.M. (1996) The Antioxidant and Antiatherogenic Effects of MAK-4 in WHHL Rabbits. Journal of Alternative and Complementary Medicine, 2, 463-478.

[25]   Sundaram, V., Hanna, A.N., Lubow, G.P., Koneru, L., Falko, J.M. and Sharma, H.M. (1997) Inhibition of LDL Oxidation by Oral Herbal Mixtures Maharishi Amrit Kalash-4 and -5 in Hyperlipidemic Patients. American Journal of the Medical Sciences, 314, 303-310.

[26]   Rock, C.L., Natarajan, L., Pu, M., Thomson, C.A., Flatt, S.W., Caan, B.J., Gold, E.B., Al-Delaimy, W.K., Newman, V.A., Hajek, R.A., Stefanick, M.L. and Pierce, J.P., for the Women’s Healthy Eating and Living Study Group (2009) Longitudinal Biological Exposure to Carotenoids Is Associated with Breast Cancer-Free Survival in the Women’s Healthy Eating and Living Study. Cancer Epidemiology, Biomarkers & Prevention, 18, 486-494.

[27]   Collins, A.R. (2005) Antioxidant Intervention as a Route to Cancer Prevention. European Journal of Cancer, 41, 1923-1930.

[28]   Dileepan, K.N., Patel, V., Sharma, H.M. and Stechschulte, D.J. (1990) Priming of Splenic Lymphocytes after Ingestion of an Ayurvedic Herbal Food Supplement: Evidence for an Immunomodulatory Effect. Biochemical Archives, 6, 267-274.