OALibJ  Vol.2 No.11 , November 2015
The Distribution and Expression of BAMBI in Breast Cancer Cell Lines
Abstract: Bmp and activin membrane-bound inhibitor (BAMBI) was initially described as a pseudo receptor antagonizing TGF-β receptor activation, thus impairing signaling. This study tries to find the expression and its significance of BAMBI in breast cancer and explore the relation between BAMBI and clinical and pathological factors of breast cancer. The expression of BAMBI was studied using three cell lines model and Immunofluorescence staining. The impact of BAMBI expression on breast cell was estimated by Western blot and Semi-quantitative RT-PCR. It was demonstrated that BAMBI protein expressed mainly in the membrane and the cytoplasm close to the membrane in breast cancer cell lines, and the relationship between the expression of BAMBI and the proliferation and migration of breast cancer for the first time. The order of BAMBI mRNA expression in the three breast cancer lines is MDA-MB-435s > MDA-MB-231 > MCF-7. Respectively the difference was significant (P < 0.05). It agrees with the proliferation and migration of the three cell lines. The relative amount of BAMBI protein in MDA-MB-435s, MDA-MB-231 and MCF-7 was 0.963 ± 0.061, 0.957 ± 0.048, 0.769 ± 0.103, and the difference was significant (P < 0.05). The abnormally up-regulation of BAMBI is related to breast carcinogenesis.
Cite this paper: Wang, H. and Cui, Z. (2015) The Distribution and Expression of BAMBI in Breast Cancer Cell Lines. Open Access Library Journal, 2, 1-7. doi: 10.4236/oalib.1102147.

[1]   Santos, R.A., Teixeira, A.C., Mayorano, M.B., Carrara, H.H., Andrade, J.M. and Takahashi, C.S. (2010) Basal Levels of DNA Damage Detected by Micronuclei and Comet Assays in Untreated Breast Cancer Patients and Healthy Women. Clinical and Experimental Medicine, 10, 87-92.

[2]   Teixeira, A.C., Dos Santos, R.A., Poersch, A., Carrara, H.H., de Andrade, J.M. and Takahashi, C.S. (2009) DNA Repair in Etoposide-Induced DNA Damage in Lymphocytes of Breast Cancer Patients and Healthy Women. International Journal of Clinical and Experimental Medicine, 2, 280-288.

[3]   Reigosa, A., Delgado, V., López, T., Avilés, Y., Balza, M., Molina, K., et al. (2006) Detección de mamoglobina y MAGE-A3 en ganglios linfáticos axilares histológicamente negativos, en cáncer de mama. Revista Venezolana de Oncologia, 18, 7.

[4]   Hanahan, D. and Weinberg, R.A. (2000) The Hallmarks of Cancer. Cell, 100, 57-70.

[5]   Thiery, J.P., Sastre-Garau, X., Vincent-Salomon, B., Sigal-Zafrani, X., Pierga, J.Y., Decraene, C., Meyniel, J.P., Gravier, E., Asselain, B., De Rycke, Y., Hupe, P., Barillot, E., Ajaz, S., Faraldo, M., Deugnier, M.A., Glukhova, M. and Medina, D. (2006) Breast Cancer Group, Challenges in the Stratification of Breast Tumors for Tailored Therapies. Bull. Cancer, 93, E81-E89.

[6]   Onichtchouk, D., Chen, Y.G., Dosch, R., et al. (1999) Silencing of TGF-Beta Signalling by the Pseudoreceptor BAMBI. Nature, 401, 480-485.

[7]   Sekiya, T., Adachi, S., Kohu, K., et al. (2004) Identification of BMP and Activin Membrane-Bound Inhibitor (BAMBI), an Inhibitor of Transforming Growth Factor-Beta Signaling, as a Target of the Beta-Catenin Pathway in Colorectal Tumor Cells. The Journal of Biological Chemistry, 279, 6840-6846.

[8]   Bai, L., Chu, G.Y. and Mai, Y. (2014) Identification and Expression Analyses of BAMBI Mediated by FSH in Swine Luteinizing Granulosa Cells. Theriogenology, 82, 1094-1101.

[9]   Pils, D. and Wittinger, M. (2010) BAMBI Is Overexpressed in Ovariancancer and Co-Translocates with Smads into the Nucleus upon TGF-β Treatment. Gynecologic Oncology, 1177, 189-197.

[10]   Balemans, W. and Van Hul, W. (2002) Extracellular Regulation of BMP Signaling in Vertebrates: A Cocktail of Modulators. Developmental Biology, 250, 231-250.

[11]   Tsang, M., Kim, R., de Caestecker, M.P., et al. (2000) Zebrafish nma Is Involved in TGF-Beta Family Signaling. Genesis, 28, 47-57.<47::AID-GENE20>3.0.CO;2-S

[12]   Karaulanov, E., Knochel, W., Niehrs, C., et al. (2004) Transcription Regulation of BMP-4 Synexpression in Transgenic Xenopus. The EMBO Journal, 23, 844-856.

[13]   Knight, C., Simmons, D., Gu, T.T., et al. (2001) Cloning, Characterization, and Tissue Expression Pattern of Mouse Nma/BAMBI during Odontogenesis. Journal of Dental Research, 80, 1895-1902.

[14]   Knight, C., Papagerakis, P., Simmons, D., et al. (2002) Genomic Organization and Localization of Mouse Nma/ BAMBI: Possible Implications Related to Ameloblastoma Formation. Connective Tissue Research, 43, 359-364.

[15]   Sasaki, T., Sasahira, T., Shimura, H., et al. (2004) Effect of Nma on Growth Inhibition by TGF-Beta in Human Gastric Carcinoma Cell Lines. Oncology Reports, 11, 1219-1223.