ABSTRACT The effect of oral L-ornithine hydrochloride (0.1 g/kg BW) on energy expenditure during a rest period from 120 to 180 min after resistance exercise was evaluated by indirect calorimetry. Healthy male subjects who have no habit of resistance training underwent resistance exercise (chest press, lat pulldown, leg press, shoulder press, leg extension, and leg curl), with 3 sets of each exercise and 10 repetitions in each set at 90-s intervals, 30 min after ingestion of ornithine or placebo. Plasma ornithine levels immediately after, and 120 and 180 min after, resistance exercise were significantly greater after ingestion of ornithine than of placebo (Treatment: F = 347.1, P < 0.001, ηp2 = 0.98; Time: F = 175.7, P < 0.001, ηp2 = 0.95; Interaction: F = 160.7, P < 0.001, ηp2 = 0.95), but no significant difference in serum growth hormone levels was observed between the two treatments (Treatment: F = 0.1, P = 0.751, ηp2 = 0.01; Time: F = 1.7, P = 0.229, ηp2 = 0.16; Interaction: F = 2.4, P = 0.155, ηp2 = 0.21). Although there was no between-treatment difference in energy expenditure during the rest period 120 to 180 min after resistance exercise (Treatment: F = 0.1, P = 0.718, ηp2 = 0.02; Time: F = 0.1, P = 0.767, ηp2 = 0.01; Interaction: F = 0.1, P = 0.112, ηp2 = 0.26), with ornithine ingestion carbohydrate oxidation was significantly greater than with placebo from 170 to 180 min after exercise (Treatment: F = 0.8, P = 0.383, ηp2 = 0.09; Time: F = 9.7, P = 0.013, ηp2 = 0.52; Interaction: F = 5.8, P = 0.039, ηp2 = 0.39). Moreover, 180 min after exercise, serum free fatty acid levels after ornithine ingestion were lower than after placebo (Treatment: F = 0.3, P = 0.602, ηp2 = 0.03; Time: F = 34.6, P < 0.001, ηp2 = 0.79; Interaction: F = 5.6, P = 0.042, ηp2 = 0.38). A similar trend in 3-hydroxybutylate was observed. In conclusion, ornithine ingestion before resistance exercise may enhance post-exercise carbohydrate oxidation without changing total energy expenditure.
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nullMorishita, K. , Yamada, T. , Yamaji, S. , Aoki, M. , Kitabayashi, T. and Uchiyama, M. (2011) The effect of ornithine ingestion on carbohydrate metabolism during rest after acute resistance exercise in healthy young males. Advances in Bioscience and Biotechnology, 2, 287-292. doi: 10.4236/abb.2011.24041.
 Chatzinikolaou, A., Fatouros, I., Petridou, A., et al., (2008) Adipose tissue lipolysis is upregulated in lean and obese men during acute resistance exercise. Diabetes Care, 31, 1397–1399.
 Yarasheski, K.E., (2008) Review Article: Exercise, Aging, and Muscle Protein Metabolism. The Journals of Gerontology, Series A: Biological Sciences, 58, M918-M922.
 Pollock, M., Gaesser, G., Butcher, J., et al., (1998) American College of Sports Medicine Position Stand. The recommended quantity and quality of exercise for developing and maintaining cardiorespiratory and muscular fitness, and flexibility in healthy adults. Medicine & Science in Sports & Exercise, 30, 975–991.
 Binzen, C., Swan, P. and Manore, M. (2001) Postexercise oxygen consumption and substrate use after resistance exercise in women. Medicine & Science in Sports & Exercise, 33, 932–938.
 Melby, C., Scholl, C., Edwards, G.., et al., (1993) Effect of acute resistance exercise on postexercise energy expenditure and resting metabolic rate. Journal of Applied Physiology, 75: 1847–1853.
 Ormsbee, M., Thyfault, J., Johnson, E., et al., (2007) Fat metabolism and acute resistance exercise in trained men. Journal of Applied Physiology, 102, 1767–1772.
 Melanson, E., Sharp, T., Seagle, H., et al., (2002) Resistance and aerobic exercise have similar effects on 24-h nutrient oxidation. Medicine & Science in Sports & Exercise, 34, 1793–1800.
 Kraemer, W.J., Marchitelli, L., Gordon, S.E., et al., (1990) Hormonal and growth factor responses to heavy resistance exercise protocols. Journal of Applied Physiology, 69, 1442–1450.
 M?ller, N., J?rgensen, J.O., Alberti, K.G,. et al., (1990) Short-term effects of growth hormone on fuel oxidation and regional substrate metabolism in normal man. The Journal of Clinical Endocrinology & Metabolism, 70, 1179–1186.
 Quisth, V., Enoksson, S., Blaak, E., et al., (2005) Major differences in noradrenaline action on lipolysis and blood flow rates in skeletal muscle and adipose tissue in vivo. Diabetologia, 48, 946–953.
 Bucci, L., Hickson, J.F., Pivarnik, J.M., et al., (1990) Ornithine ingestion and growth hormone release in bodybuilders. Nutrition Research, 10, 239–245.
 Welbourne, T.C. (1995) Increased plasma bicarbonate and growth hormone after an oral glutamine load. American Journal of Clinical Nutrition, 61: 1058–1061
 Besset, A., Bonardet, A., Rondouin, G,. et al., (1982) Increase in sleep related GH and Prl secretion after chronic arginine aspartate administration in man. Acta Endocrinologica, 99, 18–23
 Demura, S., Yamada, T., Yamaji, S., et al., (2010) The effect of L-ornithine hydrochloride ingestion on human growth hormone secretion after strength training. Advances in Bioscience and Biotechnology, 1, 7–11.
 Thyfault, J.P., Carper, M.J., Richmond, S.R., et al., (2004) Effects of liquid carbohydrate ingestion on markers of anabolism following high-intensity resistance exercise. The Journal of Strength & Conditioning Research, 18, 174–179.
 Manetta, J., Brun, J.F., Perez-Martin, A., et al., (2002) Fuel oxidation during exercise in middle-aged men: role of training and glucose disposal. Medicine & Science in Sports & Exercise, 34, 423–429.
 Beidleman, B.A., Rock, P.B., Muza, S.R., et al., (2002) Substrate oxidation is altered in women during exercise upon acute altitude exposure. Medicine & Science in Sports & Exercise, 34, 430–437.
 Gravh?lt, C.H., Schmitz, O., Simonsen, L., et al., (1999) Effects of a physiological GH pulse on interstitial glycerol in abdominal and femoral adipose tissue. American Journal of Physiology, 277, E848–E854.
 Ferrannini, E. (1899) The theoretical bases of indirect calorimetry: A review. Metabolism, 37, 287–301.
 Jéquier, E. and Felber, J. (1987) 7 Indirect calorimetry. Baillière’s Clinical Endocrinology and Metabolism, 1, 911–935