OALibJ  Vol.2 No.9 , September 2015
Bacteria Isolated from the Oropharynxs of Apparently Healthy Chickens in Jos, Nigeria
Abstract: There are concerns that apparently healthy chickens may transmit potentially pathogenic pathogens that could threaten not only poultry industry, but also public health. This investigative study was carried out in Jos, Nigeria to determine the prevalent bacterial agents in the oropharynxs of apparently healthy chickens. Four hundred (400) oropharyngeal swabs were collected from 400 apparently healthy chickens for bacteriological analysis. Swab from each sample was cultured on 7% defibrinated sheep blood, Casein Sucrose Yeast (CSY) agar and MacConkey. Oropharyngeal swabs were cultured indirectly by inoculating into 5 ml of brain heart infusion broth (BHI), incubated at 37℃ for 24 h and then streaked unto Casein Sucrose Yeast (CSY) agar, MacConkey agar and Blood agar. Presumptive colonies of bacterial agents were subjected to conventional biochemical characterization. The result of biochemical test identified the following bacteria: Staphylococcus aureus 82 (20.5%), Escherichia coli 53 (13.3%), Klebsiella pneumonia 35 (8.8%) and Proteus species 26 (6.6%), Pasteurella multocida 5 (1.3%) among others. Staphylococcus aureus (20.5%) was the highest followed by Escherichia coli (13.3%), Klebsiella pneumoniae (8.8%) and Pasteurella multocida (1.3%) was the least. It was concluded that the oropharynxs of apparently healthy chickens harbored a lot of bacterial agents which could cause infections when the immune system was compromised.
Cite this paper: Dashe, Y. , Raji, M. , Abdu, P. , Oladele, B. and Oluwadare, L. (2015) Bacteria Isolated from the Oropharynxs of Apparently Healthy Chickens in Jos, Nigeria. Open Access Library Journal, 2, 1-5. doi: 10.4236/oalib.1101808.

[1]   Glisson, J.R., Hofacre, C.L. and Christensen, J.P. (2003) Avian Pasteurellosis. In: Saif, Y.M., Barnes, H.J., Glison, J.R., Fadly, A.M., McDougald, L.R. and Swayne, D.E., Eds., Disease of Poultry, 11th Edition, , Iowa State University Press, Ames, 658-676.

[2]   Alexander, D. J. (2000) A Review of Avian Influenza in Different Bird Species. Veterinary Microbiology, 74, 3-13.

[3]   Orajaka, L.J.E. and Mohan, K. (1985) Aerobic Bacterial Flora from Dead-in-Shell Chicken Embryo from Nigeria. Avian Diseases, 29, 583-589.

[4]   Glunder, G. and Siegmann, O. (1989) Occurence of Aeromonas hydrophila in the Wild Birds. Avian Pathology, 18, 685-695.

[5]   Baruah, K.K., Sharma, P.K. and Bora, N.N. (2001) Fertility, Hatchability and Embryonic Mortality in Ducks. India Veterinary Journal, 78, 529-530.

[6]   Ogbonna, A.C. and Harris, D.J. (2008) Thermal Comfort in Sub-Sahara Africa: Field Study Report in Jos-Nigeria. Applied Energy, 85, 1-11.

[7]   IIoeje, N.P. (2006) A New Geography of Nigeria. New Revised Edition, Longman Nigeria PLC, Lagos, 200 p.

[8]   National Population Commission (NPC) (2006) Nigeria National Census: Population Distribution by Sex, State, LGAs and Senatorial District: 2006 Census Priority Tables (Vol. 3).

[9]   Clinical and Laboratory Standard Institute (CLSI) (2009) Procedure Manual for Laboratory Practice. 3rd Edition, CLSI-Science and Education Publishing, Wayne.

[10]   Barrow, G.I. and Felthan, R.K.A. (2004) Cowan and Steels Identification of Medical Bacteria. 4th Edition, Cambridge University Press, Cambridge, London, 50-145.

[11]   Statistical Package for Social Science (SPSS) Version 12.01. Chicago Incorporated, Illinois, United States of America.

[12]   Adele, S. B. and Raji, M. A. (1997) Retrospective Studies of Fungal and Bacterial Flora of Chicken in Zaria, Nigeria. Bulletin of Animal Health and Production in Africa, 45, 79-81.

[13]   Anonymous (2006) Food as Possible Source of Infection with Highly Pathogenic Avianinfluenza Viruses for Human and Other Mammals. Efsa Journal, 74, 29.

[14]   Andra, O., Jesus, J.H. and Duarte, C. (1998) Potential Pathogens Recovered from the Upper Respiratory Tract of Psittacine Birds. IVCVM, 1-4.

[15]   Adele, S.B., Raji, M.T. and Raji, M.A. (1999) Prevalence of Bacterial and Fungal Microflora Isolated from Some Wild and Domesticated Birds in Zaria, Nigeria. Bulletin of Animal Health and Production in Africa, 47, 127-132.

[16]   Hunter, B. and Wobeser, G. (1980) Pathology of Experimental Avian Cholera in Mallard Ducks. Avian Diseases, 24, 403-414.

[17]   Muhairwa, A.P., Christensen, J.P. and Bisgaard, M. (2000) Relationship among Pasteurellaceae Isolated from Free Ranging Chickens and Their Animal Contacts as Determined by Quantitative Phenotyping, Ribotyping and REA-Typing. Veterinary Microbiology, 78, 119-137.

[18]   Christensen, J.P. and Bisgaard, M. (2000) Fowl Cholera. Revue Scientifique et technique, 19, 626-637.

[19]   Matsumoto, M. and Strain, J.G. (1993) Pathogenicity of Pasteurella multocida: Its Variable Nature Demonstrated by in Vivo Passages. Avian Diseases, 37, 781-785.