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 AiM  Vol.6 No.7 , June 2016
Culture Filtrates of Trichoderma Isolate H921 Inhibit Magnaporthe oryzae Spore Germination and Blast Lesion Formation in Rice
Abstract: Spore germination and appressorium formation of Magnaporthe oryzae spores was completely suppressed by an ethyl acetate extract of the culture filtrate from the H921 isolate (H921-EAE-CF). Production of antifungal substance(s) in the H921-EAE-CF began to increase up to 3 days after isolate H921 incubation. Furthermore, heat treatment (105°C or 121°C) of H921-EAE-CF did not alter its inhibitory effect on M. oryzae spore germination compared to non-heat-treated H921-EAE-CF. Blast lesion formation inhibition by H921-EAE-CF was dose-dependent. Internal transcribed spacer (ITS) region sequence analysis indicated that this isolate shared similarities with species of the genera Trichoderma. This study suggests that H921-EAE-CF contains some antifungal substances that could be promising candidates for control of rice blast disease.
Cite this paper: Nguyen, Q. , Ueda, K. , Kihara, J. and Ueno, M. (2016) Culture Filtrates of Trichoderma Isolate H921 Inhibit Magnaporthe oryzae Spore Germination and Blast Lesion Formation in Rice. Advances in Microbiology, 6, 521-527. doi: 10.4236/aim.2016.67052.
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