Back
 JBM  Vol.4 No.6 , June 2016
Antimutagenic and DNA Damage Protective Activities of a Grape Extract from Vitis vinifera
Abstract:
Antimutagenic and DNA protective effect of an extract VinOserae from Vitis vinifera grapes on oxidative DNA damage was investigated. The extract’s ability to inhibit mutagenicity induced by tert-butyl hydroperoxide (t-BHP) and hydrogen peroxide (H2O2) was determined with Ames test using Salmonella typhimurium His? TA102 strain. Inhibition values of 44.2% and 67.0% were detected for t-BHP and H2O2, respectively. A protective ability of the extract against DNA strand scission induced by hydroxyl radicals was studied with plasmid pBluescript II SK(-). The analysis of DNA strand breaks in plasmid DNA showed a significant inhibition of DNA damage.
Cite this paper: Rybková, Z. , Malachová, K. , Červeň, J. , Sezimová, H. and Pečinka, P. (2016) Antimutagenic and DNA Damage Protective Activities of a Grape Extract from Vitis vinifera. Journal of Biosciences and Medicines, 4, 1-8. doi: 10.4236/jbm.2016.46001.
References

[1]   Boubaker, J., Skandrani, I., Bouhlel, I., Ben Sghaier, M., Neffati, A., Ghedira, K. and Chekir-Ghedira, L. (2010) Mutagenic, Antimutagenic and Antioxidant Potency of Leaf Extracts from Nitraria retusa. Food and Chemical Toxicology, 48, 2283-2290. http://dx.doi.org/10.1016/j.fct.2010.05.061

[2]   Carino-Cortés, R., Hernández-Ceruelos, A., Torres-Valencia, J.M., Gonzáles-Avila, M., Arriaga-Alba, M. and Madrigal-Bujaidar, E. (2007) Antimutagenicity of Stevia pilosa and Stevia eupatoria Evaluated with the Ames Test. Toxicology in Vitro, 21, 691-697. http://dx.doi.org/10.1016/j.tiv.2006.12.001

[3]   Ho, K.Y., Tsai, C.C., Chen, C.P., Huang, J.S. and Lin, C.C. (2001) Antimicrobial Activity of Honokiol and Magnolol Isolated from Magnolia officinalis. Phytotherapy Research, 15, 139-141. http://dx.doi.org/10.1002/ptr.736

[4]   Ramasamy, K. and Agarwal, R. (2008) Multitargeted Therapy of Cancer by Silymarin. Cancer Letters, 269, 352-362. http://dx.doi.org/10.1016/j.canlet.2008.03.053

[5]   Zhang, Y.M. and Rock, C.O. (2004) Evaluation of Epigallocatechin Gallate and Related Plant Polyphenols as Inhibitors of the FabG and FabI Reductases of Bacterial Type II Fatty-Acid Synthase. The Journal of Biological Chemistry, 279, 30994-31001. http://dx.doi.org/10.1074/jbc.M403697200

[6]   Kris-Etherton, P.M., Hecker, K.D., Bonanome, A., Coval, S.M., Bin-koski, A.E., Hilpert, K.F., Griel, A.E. and Etherton, T.D. (2002) Bioactive Compounds in Foods: Their Role in the Prevention of Cardiovascular Disease and Cancer. The American Journal of Medicine, 113, 71S-88S. http://dx.doi.org/10.1016/S0002-9343(01)00995-0

[7]   Manach, C., Scalbert, A., Morand, C., Rémésy, C. and Jiménez, L. (2004) Polyphenols: Food Sources and Bioavailability. The American Journal of Clinical Nutrition, 79, 727-747.

[8]   Scalbert, A. and Williamson, G. (2000) Dietary Intake and Bioavailability of Polyphenols. Journal of Nutrition, 2073S-2085S.

[9]   Verhagen, H., Rompelberg, C.J.M., Strube, M., Van Poppel, G. and Van Bladeren, P.J. (1997) Cancer Prevention by Dietary Constituents in Toxicological Perspective. Journal of Environmental Pathology, Toxicology and Oncology, 16, 343-360.

[10]   Bors, W., Michel, C. and Stettmaier, K. (1997) Antioxidant Effects of Flavonoids. Biofactors, 6, 399-402. http://dx.doi.org/10.1002/biof.5520060405

[11]   Edenharder, R. and Grünhage, D. (2003) Free Radical Scavenging Abilities of Flavonoids as Mechanism of Protection against Mutagenicity Induced by Tert-Butyl Hydroperoxide or Cumene Hydroperoxide in Salmonella typhimurium TA102. Mutation Research, 540, 1-18. http://dx.doi.org/10.1016/S1383-5718(03)00114-1

[12]   Othman, A., Ismail, A., Ghani, N.A. and Adenan, I. (2007) Antioxidant Capacity and Phenolic Content of Cocoa Beans. Food Chemistry, 100, 1523-1530. http://dx.doi.org/10.1016/j.foodchem.2005.12.021

[13]   Seyoum, A., Asres, K. and El-Fiky, F.K. (2006) Structure-Radical Scavenging Activity Relationships of Flavonoids. Phytochemistry, 67, 2058-2070.

[14]   Van Acker, S.A.B.E., Van Balen, G.P., Van den Berg, D.J., Bast, A. and Van der Vijgh, W.J.F. (1998) Influence of Iron Chelation on the Antioxidant Activity of Flavonoids. Biochemical Pharmacology, 56, 935-943. http://dx.doi.org/10.1016/S0006-2952(98)00102-6

[15]   Fernandes, F., Ramalhosa, E., Pires, P., Verdial, J., Valentao, P., Andrade, P., Bento, A. and Pereira, J.A. (2013) Vitis vinifera Leaves towards Bioactivity. Industrial Crops and Products, 43, 434-440. http://dx.doi.org/10.1016/j.indcrop.2012.07.031

[16]   Orhan, N., Aslan, M., Deliorman Orhan, D., Ergun, F. and Yesi-lada, E. (2006) In-Vivo Assessment of Antidiabetic and Antioxidant Activities of Grapevine Leaves (Vitis vinifera) in Diabetic Rats. Journal of Ethnopharmacology, 108, 280-286. http://dx.doi.org/10.1016/j.jep.2006.05.010

[17]   Bozan, B., Tosun, G. and Özcan, D. (2008) Study of Polyphenol Content in the Seeds of Red Grape (Vitis vinifera L.) Varieties Cultivated in Turkey and Their Antiradical Activity. Food Chemistry, 109, 426-430. http://dx.doi.org/10.1016/j.foodchem.2007.12.056

[18]   Dopico-Garcia, M.S., Fique, A., Guerra, L., Afonso, J.M., Pereira, O., Valentao, P., Andrade, P.B. and Seabra, R.M. (2008) Principal Components of Phenolics to Characterize Red Vinho Verde Grapes: Anthocyanins or Non-Coloured Compounds? Talanta, 75, 1190-1202. http://dx.doi.org/10.1016/j.talanta.2008.01.012

[19]   Liang, Z., Owens, C.L., Zhong, G.-Y. and Cheng, L. (2011) Polyphenolic Profiles Detected in the Ripe Berries of Vitis vinifera Germplasm. Food Chemistry, 129, 940-950. http://dx.doi.org/10.1016/j.foodchem.2011.05.050

[20]   Novaka, I., Janeiroa, P., Serugab, M. and Oliveira-Brett, A.M. (2008) Ultrasound Extracted Flavonoids from Four Varieties of Portuguese Red Grape Skins Determined by Re-verse-Phase High-Performance Liquid Chromatography with Electrochemical Detection. Analytica Chimica Acta, 630, 107-115. http://dx.doi.org/10.1016/j.aca.2008.10.002

[21]   Perestrelo, R., Lu, Y., Santos, S.A.O., Silvestre, A.J.D., Neto, C.P., Camara, J.S. and Rocha, S.M. (2012) Phenolic Profile of Sercial and Tinta Negra Vitis vinifera L. Grape Skins by HPLC-DAD-ESI-MSn. Novel Phenolic Compounds in Vitis vinifera L. Grape. Food Chemistry, 135, 94-104. http://dx.doi.org/10.1016/j.foodchem.2012.04.102

[22]   Spacil, Z., Novakova, L. and Solich, P. (2008) Analysis of Phenolic Compounds by High Performance Liquid Chromatography and Ultra Performance Liquid Chromatography. Talanta, 76, 189-199. http://dx.doi.org/10.1016/j.talanta.2008.02.021

[23]   Xia, E.-Q., Deng, G.-F., Guo, Y.-J. and Li, H.-B. (2010) Biological Activities of Polyphenols from Grapes. International Journal of Molecular Sciences, 11, 622-646. http://dx.doi.org/10.3390/ijms11020622

[24]   Iacopini, P., Baldi, M., Storchi, P. and Sebastiani, L. (2008) Catechin, Epicatechin, Quercetin, Rutin and Resveratrol in Red Grape: Content, in Vitro Antioxidant Activity and Interactions. Journal of Food Composition and Analysis, 21, 589-598. http://dx.doi.org/10.1016/j.jfca.2008.03.011

[25]   Shrikhande, A.J. (2000) Wine By-Products with Health Benefits. Food Research International, 33, 469-474. http://dx.doi.org/10.1016/S0963-9969(00)00071-5

[26]   Silva, R.C., Rigaud, J., Cheynier, V. and Chemina, A. (1991) Procyanidin Dimers and Trimers from Grape Seeds. Phytochemistry, 30, 1259-1264. http://dx.doi.org/10.1016/S0031-9422(00)95213-0

[27]   Thiruchenduran, M., Vijayan, N.A., Sawaminathan, J.K. and Devaraj, S.N. (2011) Protective Effect of Grape Seed Proanthocyanidins against Cholesterol Cholic Acid Diet-Induced Hypercholesterolemia in Rats. Cardiovasc. Pathology, 20, 361-368. http://dx.doi.org/10.1016/j.carpath.2010.09.002

[28]   Wada, M., Kido, H., Ohyama, K., Ichibangas, T., Kishikaw, N., Ohba, Y., Nakashima, M.N., Kurod, N. and Nakashima, K. (2007) Chemiluminescent Screening of Quenching Effects of Natural Colorants against Reactive Oxygen Species: Evaluation of Grape Seed, Monascus, Gardenia and Red Radish Extracts as Multi-Functional Food Additives. Food Chemistry, 101, 980-986. http://dx.doi.org/10.1016/j.foodchem.2006.02.050

[29]   Cao, W., Chen, W.J., Suo, Z.R. and Yao, Y.P. (2008) Protective Effects of Ethanolic Extracts of Buckwheat Groats on DNA Damage Caused by Hydroxyl Radicals. Food Research International, 41, 924-929. http://dx.doi.org/10.1016/j.foodres.2007.10.014

[30]   Russo, A., Acquaviva, R., Campisi, A., Sorrenti, V., Di Giacomo, C., Virgata, G., Barcellona, M.L and Vanella, A. (2000) Bioflavonoids as Antiradicals, Antioxidants and DNA Cleavage Protectors. Cell Biology and Toxicology, 16, 91-98. http://dx.doi.org/10.1023/A:1007685909018

[31]   Rybkova, Z. and Malachova K. (2011) The Use of Plasmid pBluescript for Detection of Antioxidative Activity of Plant Phenolics. Chemicke Listy, 105, 129-132.

[32]   Levin, D.E., Hollstein, M., Christman, M.F., Schwiers, E.A. and Ames, B.N. (1982) A New Salmonella Tester Strain (TA102) with A-T Base Pairs at the Site of Mutation Detects Oxidative Mutagens. Proceedings of the National Academy of Sciences of the United States of America, 79, 7445-7449. http://dx.doi.org/10.1073/pnas.79.23.7445

[33]   Levin, D.E., Hollstein, M., Christman, M.F. and Ames, B.N. (1984) Detection of Oxidative Mutagens with a New Salmonella Tester strain (TA102). Methods in Enzymology, 105, 249-254. http://dx.doi.org/10.1016/S0076-6879(84)05032-1

[34]   Mortelmans, K. and Zeiger, E. (2000) The Ames Salmonel-la/Microsome Mutagenicity Assay. Mutation Research, 455, 29-60. http://dx.doi.org/10.1016/S0027-5107(00)00064-6

[35]   Stagos, D., Kazantzoglou, G., Theofanidou, D., Kakalopoulou, G., Magiatis, P., Mitaku, S. and Kouretas, D. (2006) Activity of Grape Extracts from Greek Varieties of Vitis vinifera against Mutagenicity Induced by Bleomycin and Hydrogen Peroxide in Salmonella typhimurium Strain TA102. Mutation Research, 609, 165-175. http://dx.doi.org/10.1016/j.mrgentox.2006.06.032

[36]   Ames, B.J., McCann, J. and Yamasaki, E. (1975) Methods for Detecting Carcinogens and Mutagens with the Salmonella/Mammalian-Microsome Mutagenicity Test. Mutation Research, 31, 347-364. http://dx.doi.org/10.1016/0165-1161(75)90046-1

[37]   Malachová, K., Pavlícková, Z., Novotny, C., Svobodová, K., Lednická, D. and Musílková, E. (2006) Reduction in the Mutagenicity of Synthetic Dyes by Successive Treatment with Activated Sludge and the Ligninolytic Fungus, Irpex lacteus. Environmental and Molecular Mutagenesis, 47, 533-540. http://dx.doi.org/10.1002/em.20224

[38]   Broekhoven, L.H. and Nestmann, E.R. (1991) Statistical Analysis of the Salmonella Mutagenicity Assay. In: Krewski, D. and Franklin, C, Eds., Statistics in Toxicology, Gordon & Breach, Amsterdam, 28-34.

[39]   Margolin, B.H., Kim, B.S. and Risko, K.J. (1989) The Ames Salmonella/Microsome Mutagenicity Assay: Issues of Inference and Validation. Journal of the American Statistical Association, 84, 651-661. http://dx.doi.org/10.2307/2289645

[40]   Abràmoff, M.D., Magalhães, P.J. and Ram, S.J. (2004) Image Processing with ImageJ. Biophotonics International, 11, 36-42.

[41]   Brindzová, L., Mikulásová, M., Takácsová, M., Mosovská, S. and Opattová, A. (2009) Evaluation of the Mutagenicity and Antimutagenicity of Extracts from Oat, Buckwheat and Wheat Bran in the Salmonella/Microsome Assay. Journal of Food Composition and Analysis, 22, 87-90. http://dx.doi.org/10.1016/j.jfca.2008.07.009

[42]   Horn, R.C. and Vargas, V.M.F. (2008) Mutagenicity and Antimutagenicity of Teas Used in Popular Medicine in the Salmonella/Microsome Assay. Toxicology in Vitro, 22, 1043-1049. http://dx.doi.org/10.1016/j.tiv.2007.12.014

[43]   Makena, P.S. and Chung, K.T. (2007) Effects of Various Plant Polyphenols on Bladder Carcinogen Benzidine-Induced Mutagenicity. Food and Chemical Toxicology, 45, 1899-1909. http://dx.doi.org/10.1016/j.fct.2007.04.007

[44]   Dillon, D., Combes, R. and Zeiger, E. (1998) The Effectiveness of Salmonella Strains TA100, TA102 and TA104 for Detecting Mutagenicity of Some Aldehydes and Peroxides. Mutagenesis, 13, 19-26. http://dx.doi.org/10.1093/mutage/13.1.19

[45]   Ferrer, M., Sánchez-Lamar, A., Fuentes, J.L., Barbé, J. and Llagostera, M. (2002) Antimutagenic Mechanisms of Phyllanthus orbicularis When Hydrogen Peroxide Is Tested Using Salmonella Assay. Mutation Research, 517, 251-254. http://dx.doi.org/10.1016/S1383-5718(02)00064-5

[46]   Grey, C.E. and Adlercreutz, P. (2003) Ability of Antioxidants to Prevent Oxidative Mutations in Salmonella typhimurium TA102. Mutation Research, 527, 27-36. http://dx.doi.org/10.1016/S0027-5107(03)00054-X

[47]   Lee, E.J. and Jang, H.D. (2004) Antioxidant activity and protective effect on DNA strand scission of Rooibos tea (Aspalathus linearis). Biofactors, 21, 285-292. http://dx.doi.org/10.1002/biof.552210156

[48]   Rahman, A., Fazal, F., Greensill, J., Ainley, K., Parish, J.H. and Hadi, S.M. (1992) Strand Scission in DNA Induced by Dietary Flavonoids: Role of Cu(I) and Oxygen Free Radicals and Biological Consequences of Scission. Molecular and Cellular Biochemistry, 111, 3-9. http://dx.doi.org/10.1007/BF00229567

[49]   Yoshino, M., Haneda, M., Naruse, M. and Murakami, K. (1999) Prooxidant Activity of Flavonoids: Copper-Dependent Strand Breaks and the Formation of 8-Hydroxy-2’-Deoxyguanosine in DNA. Molecular Genetics and Metabolism, 68, 468-472. http://dx.doi.org/10.1006/mgme.1999.2901

[50]   Kong, D.M., Wang, J., Zhu, L.N., Jin, Y.W., Li, X.Z., Shen, H.X. and Mi, H.F. (2008) Oxidative DNA Cleavage by Schiff Base Tetraazamacrocyclic Oxamido Nickel(II) Complexes. Journal of Inorganic Biochemistry, 102, 824-832. http://dx.doi.org/10.1016/j.jinorgbio.2007.12.002

[51]   Liu, X., Lu, J. and Liu, S. (1999) Synergistic Induction of Hydroxyl Radical-Induced DNA Single-Strand Breaks by Chromium(VI) Compound and Cigarette Smoke Solution. Mutation Research, 440, 109-117. http://dx.doi.org/10.1016/S1383-5718(99)00015-7

[52]   Khan, N.S., Ahmad, A. and Hadi, S.M. (2000) Anti-Oxidant, Pro-Oxidant Properties of Tannic Acid and Its Binding to DNA. Chemico-Biological Interactions, 125, 177-189. http://dx.doi.org/10.1016/S0009-2797(00)00143-5

[53]   Ueda, J., Saito, N., Shimazu, Y. and Ozawa, T. (1996) A Comparison of Scavenging Abilities of Antioxidants against Hydroxyl Radicals. Archives of Biochemistry and Biophysics, 333, 377-384. http://dx.doi.org/10.1006/abbi.1996.0404

 
 
Top