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 AJPS  Vol.7 No.7 , May 2016
In Vitro Propagation Potential via Somatic Embryogenesis of the Two Maturing Early Cultivars of European Chestnut (Castanea sativa Mill.)
Abstract: Turkey ranks the third in the production of chestnuts in the world having an important place both in domestic and global markets. However, the chestnut production and the number of trees have been diminishing in recent years. Therefore, in vitro propagation of the chestnut, in addition to the classical propagation techniques, should be applied. Especially the propogation of the early maturing cultivars and production of the quality chestnuts will provide a better income to the producer. Here, somatic embryo production and regeneration from the immature cotyledons of the early maturing cultivars of the European chestnut (Castanea sativa Mill), Haciibis and Karamehmet, were studied using the somatic embryogenesis, one of the in vitro propagation techniques. To induce the somatic embryogenesis, 168 different combinations were applied to both cultivars. The somatic embryogenesis rate in Haciibis cultivar, in which the interactions were observed among the applications, was found to be 9.9% while it was 11.1% for the Karamehmet cultivar. Dessication, cold treatment, gibberellic acid (GA3) and benzyladenine (BA) + naphthaleneacetic acid (NAA) applications were performed on the regeneration of the somatic embryos, and 40% conversion to plant was obtained with desiccation together with BA + NAA supplementation to the medium.


Cite this paper: Sezgin, M. and Dumanoğlu, H. (2016) In Vitro Propagation Potential via Somatic Embryogenesis of the Two Maturing Early Cultivars of European Chestnut (Castanea sativa Mill.). American Journal of Plant Sciences, 7, 1001-1012. doi: 10.4236/ajps.2016.77095.
References

[1]   Miller, G., Miller, D.D. and Jaynes, R.A. (1996) Chestnuts. In: Janick, J. and Moore, J.N., Eds., Fruit Breeding, Nuts,Vol. 3, John Wiley and Sons, Inc., New York, 99-123.

[2]   Soylu, A. (2004) Kestane Yetistiriciligi ve Ozellikleri. Hasad Yayinlari, Istanbul, 1-57.

[3]   Vieitez, F.J. and Merkle, S.A. (2005) Castanea Spp. Chestnut. In: Litz, R.E., Ed., Biotechnology of Fruit and Nut Crops, CABI Publishing, Trowbridge, 263-265.

[4]   Corredoira, E., Ballaster, A., Vieitez, F.J. and Vieitez, A.M. (2006) Somatic Embryogenesis in Chestnut. In: Mujib, A. and Samaj, J., Eds., Somatic Embryogenesis, Springer, Berlin, 177-200. http://dx.doi.org/10.1007/7089_036

[5]   FAO (2015) Agricultural Statistics. Accession date: 4 September 2015.

[6]   Soylu, A. and Mert, C. (2009) The Future of Chestnut Breeding Work in Turkey. Acta Horticulturae, 815, 27-31.
http://dx.doi.org/10.17660/actahortic.2009.815.2

[7]   OGM (2014) Turkiye Orman Varligi. Accession date: 1 September 2015
http://www.ogm.gov.tr/Sayfalar/Ormanlarimiz/TurkiyeOrmanVarligi.aspx

[8]   Ansin, R. and Ozkan, Z.C. (1997) Tohumlu Bitkiler (Spermatophyta) Odunsu Taksonlar. Karadeniz Teknik Universitesi Orman Fakultesi Yayinlari, Trabzon.

[9]   Eroglu, Z.O., Fidanci, A. and Misirli, A. (2012) Erkenci Seftali Embriyolarinin Farkli Kultur ve Ortamlarinda Gelisimi. Tarim Bilimleri Dergisi, 18, 93-99. http://dx.doi.org/10.1501/Tarimbil_0000001196

[10]   Soylu, A., Eris, A., Ozgur, M. and Dalkilic, Z. (1999) Research on Rootstock Potentially of Chestnut Types (Castanea sativa Mill) Grown in Marmara Region. Acta Horticulturae, 494, 213-221.
http://dx.doi.org/10.17660/ActaHortic.1999.494.32

[11]   Ozcan, S., Babaoglu, M. and Sancak, C. (2002) Somatik Embriyogenesis. In: Babaoglu, M., Gurel, E. and Ozcan, S., Eds., Bitki Biyoteknolojisi I, Selcuk Universitesi Vakfi Yayinlari, Konya, 71-88.

[12]   San, B., Sezgin, M., Dumanoglu, H. and Koksal, A.I. (2007) Somatic Embryogenesis from Immature Cotyledons of Some European Chestnut (Castanea sativa Mill) Cultivars. Turkish Journal of Agriculture and Forestry, 31, 175-179.

[13]   Murashige, T. and Skoog, F. (1962) A Revised Media for Rapid Growth and Bioassay with Tobacco Tissue Cultures. Physiologia Planta, 15, 473-497. http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x

[14]   Tulecke, W. and McGranahan, G. (1985) Somatic Embryogenesis and Plant Regeneration from Cotyledons of Walnut (Juglans regia L.). Plant Science, 40, 57-63. http://dx.doi.org/10.1016/0168-9452(85)90163-3

[15]   Lloyd, G. and McCown, B.H. (1981) Commercially-Feasible Micropropagation of Mountain Laurel (Kalmia latifolia), by Shoot Tip Culture. Proceeding of the International Plant Propagation Society, 30, 421-427.

[16]   Corredoira, E., Ballester, A. and Vieitez, A.M. (2003) Proliferation, Maturation and Germination of Castanea sativa Mill. Somatic Embryos Originated from Leaf Explants. Annals of Botany, 92, 129-136.
http://dx.doi.org/10.1093/aob/mcg107

[17]   Corredoira, E., Valladares, S. and Vieitez, A.M. (2008) Improved Germination of Somatic Embryos and Plant Recovery of European Chestnut. In Vitro Cellular and Developmental Biology—Plant, 44, 307-315.
http://dx.doi.org/10.1007/s11627-008-9105-6

[18]   Vieitez, A.M., Gonzales, M.L. and Vieitez, E. (1978) In Vitro Culture of Cotyledon Tissue of Castanea sativa Mill. Scientia Horticulturae, 8, 243-247. http://dx.doi.org/10.1016/0304-4238(78)90031-6

[19]   Gonzales, M.L., Vieitez, A.M. and Vieitez, E. (1985) Somatic Embryogenesis from Chestnut Cotyledon Tissue Cultured in Vitro. Scientia Horticulture, 27, 97-103. http://dx.doi.org/10.1016/0304-4238(85)90059-7

[20]   Vieitez, F.J., San Jose, M.C., Ballester, A. and Vieitez, A.M. (1990) Somatic Embriyogenesis in Cultured Immature Zygotic Embryos in Chestnut. Journal of Plant Physiology, 136, 253-256.
http://dx.doi.org/10.1016/S0176-1617(11)81675-5

[21]   Merkle, S.A., Wiecko, A.T. and Watson-Pauley, B.A. (1991) Somatic Embryogenesis in American Chestnut. Canadian Journal of Forest Research, 21, 1698-1701. http://dx.doi.org/10.1139/x91-235

[22]   Carraway, D.T. and Merkle, S.A. (1997) Plantlet Regeneration from Somatic Embryos of American Chestnut. Canadian Journal of Forest Research, 27, 1805-1812. http://dx.doi.org/10.1139/x97-123

[23]   Xing, Z., Powell, W.A. and Maynard, C.A. (1999) Development and Germination of American Chestnut Somatic Embryos. Plant Cell, Tissue and Organ Culture, 57, 47-55. http://dx.doi.org/10.1023/A:1006360730898

[24]   Robichaud, R.L., Lessard, V.C. and Merkle, S. (2004) A Treatments Effecting Maturation and Germination of American Chestnut Somatic Embryos. Journal of Plant Physiology, 161, 957-969.
http://dx.doi.org/10.1016/j.jplph.2004.03.003

[25]   Andrade, G.M. and Merkle, S.A. (2005) Enhancement of American Chestnut Somatic Seedling Production. Plant Cell Reports, 24, 326-334. http://dx.doi.org/10.1007/s00299-005-0941-0

[26]   Sauer, U. and Wilhelm, E. (2005) Somatic Embryogenesis from Ovaries, Developing Ovules and Immature Zigotic Embryos and Improved Embryo Development of Castanea sativa. Biologia Plantarum, 49, 1-6.
http://dx.doi.org/10.1007/s10535-005-1006-5

[27]   Sezgin, M. and Dumanoglu, H. (2014) Somatic Embryogenesis and Plant Regeneration from Immature Cotyledons of European Chestnut (Castanea sativa Mill.). In Vitro Cellular and Developmental Biology—Plant, 50, 58-68.
http://dx.doi.org/10.1007/s11627-013-9548-2

[28]   Winkelmann, T., Meyer, L. and Serek, M. (2004) Desiccation of Somatic Embryos of Cyclamen persicum Mill. The Journal of Horticultural Science and Biotechnology, 79, 479-483. http://dx.doi.org/10.1080/14620316.2004.11511793

[29]   Gray, D.J. (1989) Affects of Dehydration and Exogenous Growth Regulators on Dormancy Quiescence and Germination of Grape Somatic Embryos. In Vitro Cellular and Developmental Biology—Plant, 25, 1173-1178.
http://dx.doi.org/10.1007/BF02621270

[30]   Kermode, A.R. and Bewley, D.J. (1985) The Role of Maturation Drying in the Transition from Seed Development to Germination. Journal of Experimental Botany, 36, 1916-1927. http://dx.doi.org/10.1093/jxb/36.12.1916

[31]   George, F.E., Hall, M.A. and De Klerk, G.-J. (2008) Somatic Embryogenesis. In: George, F.E., Hall, M.A. and De Klerk, G.-J., Eds., Plant Propagation by Tissue Culture, Springer, Dordrecht, 335-354.

[32]   Kepczynska, E. and Zielinska, S. (2006) Regulation of Medicago sativa L. Somatic Embryos Regeneration by Gibberellins GA3 and Abscisic Acid in Relation to Starch Content and Alpha-Amylase Activity. Plant Growth Regulation, 29, 209-217. http://dx.doi.org/10.1007/s10725-006-9106-6

 
 
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