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 AiM  Vol.6 No.4 , April 2016
Detection of Listeria monocytogenes in Foods and Characterization by PFGE
Abstract: The aims of the present study were to investigate the prevalence of Listeria monocytogenes in 1042 foods collected from different market to characterize the isolates by phenotypical and molecular methods. In particular, L. monocytogenes obtained from different types of foods such as RTE (kimbap), fish (smoked salmon and seasoned-dried slice fish) and meat (cut raw beef and pork) from 2009 to 2011, were used. Twelve samples (2.1%) were positive for L. monocytogenes. Detection rate of L. monocytogenes varied significantly by food type and ranged from 1.1% to 5.2%. Meat is the highest prevalence for L. monocytogenes (5.2%) followed by RTE (1.8%) and Fish (1.1%). Twelve isolates were also serotyped by the agglutination method. The most common serotypes detected in the 12 strains tested were 1/4b (75.0%), followed by 1/2a (16.7%), and 1/2b (8.3%). For this study, we used serotyping and detected 6 different virulence-associated genes (inlA, inlB, plcA, plcB, hlyA, and actA) and 16s rRNA using multiplex-PCR. PFGE was performed to determine genetic characterization of L. monocytogenes strains to define the genetic diversity.
Cite this paper: Park, S. , Jung, H. , Lee, M. , Choi, H. , Kim, J. , Jung, J. , Park, S. , Kim, M. , Kim, K. , Oh, Y. , Chung, A. and Jung, K. (2016) Detection of Listeria monocytogenes in Foods and Characterization by PFGE. Advances in Microbiology, 6, 343-349. doi: 10.4236/aim.2016.64033.
References

[1]   Scallan, E., Hoekstra, R.M., Angulo, F.J., Tauxe, R.V., Widdowson, M.A., Roy, S.L., Jones, J.L. and Griffin, P.M. (2011) Foodborne Illness Acquired in the United States Major Pathogens. Emerging Infectious Diseases, 17, 7-15.
http://dx.doi.org/10.3201/eid1701.P11101

[2]   Todd, E.C.D. and Notermans, S. (2011) Surveillance of listeriosis and Its Causative Pathogen, Listeria monocytogenes. Food Control, 22, 1484-1490.
http://dx.doi.org/10.1016/j.foodcont.2010.07.021

[3]   Ferreira, V., Wiedmann, M., Teixeira, P. and Stasiewicz, M.J. (2014) Listeria monocytogenes Persistence in Food-Associated Environments: Epidemiology, Strain Characteristics, and Implications for Public Health. Journal of Food Protection, 77, 150-170.
http://dx.doi.org/10.4315/0362-028X.JFP-13-150

[4]   Lomonaco, S., Nucera, D. and Filipello, V. (2015) The Evolution and Epidemiology of Listeria monocytogenes in Europe and the United States. Infection, Genetics and Evolution, 35, 172-183.
http://dx.doi.org/10.1016/j.meegid.2015.08.008

[5]   Bonazzi, M., Lecuit, M. and Cossart, P. (2009) Listeria monocytogenes Internalin and Ecadherin: from Structure to Pathogenesis. Cellular Microbiology, 11, 693-702.
http://dx.doi.org/10.1111/j.1462-5822.2009.01293.x

[6]   Fenlon, D.R., Wilson, J. and Donachie, W. (1996) The Incidence and Level of Listeria monocytogenes Contamination of Food Sources at Primary Production and Initial Processing. Journal of Applied Bacteriology, 81, 641-650.
http://dx.doi.org/10.1111/j.1365-2672.1996.tb03559.x

[7]   Autio, T., Hielm, S., Miettinen, M., Sjoberg, A.M., Aarnisalo, K., Bjorkroth, J., Mattila, S.T. and Korkeala, H. (1999) Sources of Listeria monocytogenes Contamination in a Cold-Smoked Rainbow Trout Processing Plant Detected by Pulsed-Field Gel Electrophoresis Typing. Applied and Environmental Microbiology, 65, 150-155.

[8]   Johansson, T., Rantala, L., Palmu, L. and Honkanen-Buzalki, T. (1999) Occurrence and Typing of Listeria monocytogenes Strains in Retail Vacuum-Packed Fish and in a Production Plant. International Journal of Food Microbiology, 47, 111-119.
http://dx.doi.org/10.1016/S0168-1605(99)00019-7

[9]   Miettinen, M.K., Bjorkroth, K.J. and Korkeala, H.J. (1999) Characterization of Listeria monocytogenes from an Ice Cream Plant by Serotyping and Pulsed-Field Gel Electrophoresis. International Journal of Food Microbiology, 46, 187-192.
http://dx.doi.org/10.1016/S0168-1605(98)00185-8

[10]   Norton, D.M., McCamey, M.A., Gall, K.L., Scarlett, J.M., Boor, K.J. and Wiedmann, M. (2001) Molecular Studies on the Ecology of Listeria monocytogenes in the Smoked Fish Processing Industry. Applied and Environmental Microbiology, 67, 198-205.
http://dx.doi.org/10.1128/AEM.67.1.198-205.2001

[11]   Hoffman, A.D., Gall, K.L., Norton, D.M. and Wiedmann, M. (2003) Listeria monocytogenes Contamination Patterns for the Smoked Fish Processing Environment and for Raw Fish. Journal of Food Protection, 66, 52-60.

[12]   Heir, E., Lindstedt, B.A., Røtterud, O.J., Vardund, T., Kapperud, G. and Nesbakken, T. (2004) Molecular Epidemiology and Disinfectant Susceptibility of Listeria monocytogenes from Meat Processing Plants and Human Infections. International Journal of Food Microbiology, 96, 85-96.
http://dx.doi.org/10.1016/j.ijfoodmicro.2004.03.014

[13]   Rawool, D.B., Malik, S.V., Shakuntala, I., Sahare, A.M. and Barbuddhe, S.B. (2007) Detection of Multiple Virulence-Associated Genes in Listeria monocytogenes Isolated from Bovine Mastitis Cases. International Journal of Food Microbiology, 113, 201-207.

[14]   Riedo, F.X., Weaver, R.E., Plikaytis, B.D. and Broome, C.V. (1994) A Point-Source Food Borne Listeriosis Outbreak: Documented Incubation Period and Possible Mild Illness. The Journal of Infectious Diseases, 170, 693-696.
http://dx.doi.org/10.1093/infdis/170.3.693

[15]   Yong, S.J., Joseph, F., Frank, R., Brackett, E. and Jinru, C. (2003) Polymerase Chain Reaction Detection of Listeria monocytogenes on Frankfurters Using Oligonucleotide Primers Targeting the Genes Encoding Internalin AB. Journal of Food Protection, 66, 237-241.

[16]   Gianfranceschi, M., Fransiosa, G., Gattuso, A. and Aureli, P. (1998) Detection of Two Phospholipases C by Means of Plate Tests for the Rapid Identification of Pathogenic Listeria monocytogenes. Archiv fur Lebensmittelhygiene, 49, 54-57.

[17]   Winters, D.K., Maloney, T.P. and Johnson, M.G. (1999) Rapid Detection of Listeria monocytogenes by a PCR Assay Specific for an Aminopeptidase. Molecular and Cellular Probes, 13, 127-131.
http://dx.doi.org/10.1006/mcpr.1999.0224

[18]   Vázquez-Boland, J.A., Domínguez-Bernal, G., González-Zorn, B., Kreft, J. and Goebel, W. (2001) Pathogenicity Islands and Virulence Evolution in Listeria. Microbes and Infection, 3, 571-584.
http://dx.doi.org/10.1016/S1286-4579(01)01413-7

[19]   Graves, L.M., Hunter, S.B., Ong, A.R., Schoonmaker-Bopp, D., Hise, K., Kornstein, L., De Witt, W.E., Hayes, P.S., Dunne, E., Mead, P. and Swaminathan, B. (2005) Microbiological Aspects of the Investigation That Traced the 1998 Outbreak of Listeriosis in the United States to Contaminated Hot Dogs and Establishment of Molecular Subtyping-Based Surveillance for Listeria monocytogenes in the PulseNet Network. Journal of Clinical Microbiology, 43, 2350- 2355.
http://dx.doi.org/10.1128/JCM.43.5.2350-2355.2005

[20]   Graves, L.M. and Swaminathan, B. (2001) PulseNet Standardized Protocol for Subtyping Listeria monocytogenes by Macrorestriction and Pulsed-Field Gel Electrophoresis. International Journal of Food Microbiology, 65, 55-62.
http://dx.doi.org/10.1016/S0168-1605(00)00501-8

[21]   Wagner, M., Auer, B., Trittremmel, C., Hein, I. and Schoder, D. (2007) Survey of the Listeria Contamination of Ready-to-Eat Food Products and Household Environments in Vienna, Austria. Zoonoses and Public Health, 54, 16-22.
http://dx.doi.org/10.1111/j.1863-2378.2007.00982.x

[22]   Allerberger, F. and Wagner, M. (2010) listeriosis: A Resurgent Foodborne Infection. Clinical Microbiology and Infection, 16, 16-23.
http://dx.doi.org/10.1111/j.1469-0691.2009.03109.x

[23]   European Food Safety Authority (2009) The Community Summary Report on Trends and Sources of Zoonoses and Zoonotic Agents in the European Union in 2007. European Food Safety Authority Journal, 223, 118-141.

[24]   Tappero, J.W., Schuchat, A., Deaver, K.A., Mascola, L. and Wenger, J.D. (1995) Reduction in the Incidence of Human listeriosis in the United States: Effectiveness of Prevention Efforts? The Journal of the American Medical Association, 273, 1118-1122.
http://dx.doi.org/10.1001/jama.1995.03520380054035

[25]   Graves, L.M., Hunter, S.B., Ong, A.R., Schoonmaker-Bopp, D., Hise, K., Kornstein, L., DeWitt, W.E., Hayes, P.S., Dunne, E., Mead, P. and Swaminathan, B. (2005) Microbiological Aspects of the Investigation That Traced the 1998 Outbreak of listeriosis in the United States to Contaminated Hot Dogs and Establishment of Molecular Subtyping-Based Surveillance for Listeria monocytogenes in the PulseNet Network. Journal of Clinical Microbiology, 43, 2350-2355.
http://dx.doi.org/10.1128/JCM.43.5.2350-2355.2005

[26]   Bille, J. and Rocourt, J. (1996) WHO International Multicenter Listeria Monocytogenes Subtyping Study-Rationale and Set-Up of the Study. International Journal of Food Microbiology, 32, 251-262.
http://dx.doi.org/10.1016/S0168-1605(96)01140-3

[27]   Suihko, M.L., Salo, S., Niclasen, O., Gudbjörnsdóttir, B., Torkelsson, G., Bredholt, S., Sjöberg, A.M. and Gustavsson, P. (2002) Characterization of Listeria monocytogenes Isolates from the Meat, Poultry and Seafood Industries by Automated Ribotyping. International Journal of Food Microbiology, 72, 137-146.
http://dx.doi.org/10.1016/S0168-1605(01)00631-6

[28]   Martinez, I., Rørvik, L.M., Brox, V., Lassen, J., Seppola, M., Gram, L. and Fonnesbech-Vogel, B. (2003) Genetic Variability among Isolates of Listeria monocytogenes from Food Products, Clinical Samples and Processing Environments, Estimated by RAPD Typing. International Journal of Food Microbiology, 84, 285-297.
http://dx.doi.org/10.1016/S0168-1605(02)00423-3

[29]   Autio, T., Lundén, J., Fredriksson-Ahomaa, M., Björkroth, J., Sjöberg, A.M. and Korkeala, H. (2002) Similar Listeria monocytogenes Pulsotypes Detected in Several Foods Originating from Different Sources. International Journal of Food Microbiology, 77, 83-90.
http://dx.doi.org/10.1016/S0168-1605(02)00055-7

[30]   Margolles, A., Mayo, B. and de los Reyes-Gavilan, C.G. (1998) Polymorphism of Listeria monocytogenes and Listeria innocua Strains Isolated from Short-Ripened Cheeses. Journal of Applied Microbiology, 84, 255-262.
http://dx.doi.org/10.1046/j.1365-2672.1998.00339.x

[31]   Nadon, C.A., Woodward, D.L., Young, C., Rodgers, F.G. and Wiedmann, M. (2001) Correlations between Molecular Subtyping and Serotyping of Listeria monocytogenes. Journal of Clinical Microbiology, 39, 2704-2707.
http://dx.doi.org/10.1128/JCM.39.7.2704-2707.2001

[32]   Giovannacci, I., Ragimbeau, C., Queguiner, S., Salvat, G., Vendeuvre, J.L., Carlier, V. and Ermel, G. (1999) Listeria monocytogenes in Pork Slaughtering and Cutting Plants: Use of RAPD, PFGE and PCR-REA for Tracing and Molecular Epidemiology. International Journal of Food Microbiology, 53, 127-140.
http://dx.doi.org/10.1016/S0168-1605(99)00141-5

[33]   Wallace, F.M., Call, J.E., Porto, A.C., Cocoma, G.J. and Luchansky, J.B. (2003) Recovery Rate of Listeria monocytogenes from Commercially Prepared Frankfurters during Extended Refrigerated Storage. Journal of Food Protection, 66, 584-591.

[34]   Okwumabua, O., O’Connor, M., Shull, E., Strelow, K., Hamacher, M., Kurzynski, T. and Warshauer, D. (2005) Characterization of Listeria monocytogenes Isolates from Food Animal Clinical Cases: PFGE Pattern Similarity to Strains from Human listeriosis Cases. FEMS Microbiology Letters, 249, 275-281.
http://dx.doi.org/10.1016/j.femsle.2005.06.018

[35]   Chung, H.C. and Wang, C. (2006) Genetic Relatedness between Listeria monocytogenes Isolates from Seafood and Humans Using PFGE and REP-PCR. International Journal of Food Microbiology, 110, 135-148.
http://dx.doi.org/10.1016/j.ijfoodmicro.2006.02.003

 
 
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