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 AiM  Vol.6 No.4 , April 2016
Inhibition of Curcumin-Treated Herpes Simplex Virus 1 and 2 in Vero Cells
Abstract: The purpose of this study was to investigate the effect of curcumin-treated Herpes simplex virus-1 (HSV-1) and Herpes simplex virus-2 (HSV-2) virions in cultured Vero cells. Previous studies have indicated that curcumin, a polyphenol extracted from the plant Curcuma longa, has demonstrated antiviral properties against a variety of viruses. After establishing the maximum non-cytotoxic concentrations of curcumin on Vero cells, HSV-1 and HSV-2 virions were treated with varying concentrations of curcumin. The effect on infectivity was determined by antiviral assays, using WST-1, plaque assays, adsorption and penetration assays. Treating HSV-1 and HSV-2 viruses with curcumin, at a concentration of 30 μM, reduces the production of infectious HSV-1 and HSV-2 virions in cultured Vero cells by interfering with the adsorption process. These results support the potential of curcumin to be used as a therapeutic agent to reduce the transmission of HSV-1 and HSV-2.
Cite this paper: Flores, D. , Lee, L. and Adams, S. (2016) Inhibition of Curcumin-Treated Herpes Simplex Virus 1 and 2 in Vero Cells. Advances in Microbiology, 6, 276-287. doi: 10.4236/aim.2016.64027.
References

[1]   Nahmias, A.J., Lee, F.K. and Beckman-Nahmias, S. (1990) Sero-Epidemiological and -Sociological Patterns of Herpes Simplex Virus Infection in the World. Scandinavian Journal of Infectious Diseases Supplementum, 69, 19-36.

[2]   Lafferty, W.E., Coombs, R.W., Benedetti, J., Critchlow, C. and Corey, L. (1987) Recurrences after Oral and Genital Herpes Simplex Virus Infection. Influence of Site of Infection and Viral Type. New England Journal of Medicine, 316, 1444-1449.
http://dx.doi.org/10.1056/nejm198706043162304

[3]   Akhtar, J. and Shukla, D. (2009) Viral Entry Mechanisms: Cellular and Viral Mediators of Herpes Simplex Virus Entry. Federation of European Biochemical Societies Journal, 276, 7228-7236.
http://dx.doi.org/10.1111/j.1742-4658.2009.07402.x

[4]   Garner, J.A. (2003) Herpes Simplex Virion Entry into and Intracellular Transport within Mammalian Cells. Advanced Drug Delivery Reviews, 55, 1497-1513.
http://dx.doi.org/10.1016/j.addr.2003.07.006

[5]   O’Donnell, C.D., Kovacs, M., Akhtar, J., Valyi-Nagy, T. and Shukla, D. (2010) Expanding the Role of 3-O Sulfated Heparan Sulfate in Herpes Simplex Virus Type-1 Entry. Virology, 397, 389-398.
http://dx.doi.org/10.1016/j.virol.2009.11.011

[6]   Whitley, R.J., Kimberlin, D.W. and Roizman, B. (1998) Herpes Simplex Viruses. Clinical Infectious Diseases, 26, 541-555.
http://dx.doi.org/10.1086/514600

[7]   Hafezi, W., Lorentzen, E.U., Eing, B.R., Müller, M., King, N.J.C., Klupp, B., Metenleiter, T.C. and Kühn, J.E. (2012) Entry of Herpes Simplex Virus Type 1 (HSV-1) into the Distal Axons of Trigeminal Neurons Favors the Onset of Nonproductive, Silent Infection. PLoS Pathogens, 8, e1002679.
http://dx.doi.org/10.1371/journal.ppat.1002679

[8]   Lafferty, W.E., Downey, L., Celum, C. and Wald, A. (2000) Herpes Simplex Virus Type 1 as a Cause of Genital Herpes: Impact on Surveillance and Prevention. The Journal of Infectious Diseases, 181, 1454-1457.
http://dx.doi.org/10.1086/315395

[9]   Biskup, U.G., Ursic, T. and Petrovec, M. (2015) Laboratory Diagnosis and Epidemiology of Herpes Simplex 1 and 2 Genital Infections. Acta Dermatovenerologica, 24, 31-35.

[10]   Schiffer, J.T. and Corey, L. (2009) New Concepts in Understanding Genital Herpes. Current Infectious Disease Reports, 11, 457-464.
http://dx.doi.org/10.1007/s11908-009-0066-7

[11]   Xu, F., Schillinger, J.A., Sternberg, M.R., Johnson, R.E., Lee, F.K., Nahmias, A.J. and Markowitz, L.E. (2002) Seroprevalence and Coinfection with Herpes Simplex Virus Type 1 and Type 2 in the United States, 1988-1994. The Journal of Infectious Diseases, 185, 1019-1024.
http://dx.doi.org/10.1086/340041

[12]   Xu, F., Sternberg, M.R., Kottiri, B.J., McQuillan, G.M., Lee, F.K., Nahmias, A.J., Berman, S.M. and Markowitz, L.E. (2006) Trends in Herpes Simplex Virus Type 1 and Type 2 Seroprevalence in the United States. JAMA, 296, 964-973.
http://dx.doi.org/10.1001/jama.296.8.964

[13]   Looker, K.J., Garnett, G.P. and Schmid, G.P. (2008) An Estimate of the Global Prevalence and Incidence of Herpes Simplex Virus Type 2 Infection. Bulletin of the World Health Organization, 86, 805-812.
http://dx.doi.org/10.2471/BLT.07.046128

[14]   Weiss, H., Buvé, A., Robinson, N., Van Dyck, E., Kahindo, M., Anagonou, S., Musonda, R., Zekeng, L., Morison, L., Caraël, M., Laga, M. and Hayes, R.J. (2001) The Epidemiology of HSV-2 Infection and Its Association with HIV Infection in Four Urban African Populations. AIDS, 15, S97-S108.
http://dx.doi.org/10.1097/00002030-200108004-00011

[15]   Fujie, X., Lee, F.K., Morrow, R.A., Sternberg, M.R., Luther, K.E., Dubin, G. and Markowitz, L.E. (2007) Seroprevalence of Herpes Simplex Virus Type 1 in Children in the United States. Journal of Pediatrics, 151, 374-377.
http://dx.doi.org/10.1016/j.jpeds.2007.04.065

[16]   Uribe-Salas, F., Palma-Coca, O., Sánchez-Alemán, M.A., Olamendi, M., Juárez-Figueroa, L. and Conde-Glez, C.J. (2009) Population-Based Prevalence of Antibodies against Herpes Simplex Virus Type 2 and Socio-Demographic Characteristic of Mexico. Transactions of the Royal Society of Tropical Medicine and Hygiene, 103, 151-158.
http://dx.doi.org/10.1016/j.trstmh.2008.10.033

[17]   Waweru, C.W. (2008) PIN 46 Country Assessment to Determine Factors Influencing the Cost, Availability and Distribution of Acyclovir in Eight Sub-Saharan African Countries. Value in Health, 11, A106-A107.
http://dx.doi.org/10.1016/S1098-3015(10)70344-2

[18]   Cantatore, A.C., Randall, S.D., Traum, D. and Adams, S.D. (2013) Effect of Black Tea Extract on Herpes Simplex Virus-1 Infection of Cultured Cells. BMC Complementary and Alternative Medicine, 13, 139.
http://dx.doi.org/10.1186/1472-6882-13-139

[19]   de Oliveira, A., Adams, S.D., Lee, L.H., Murray, S.R., Hsu, S.D., Hammond, J.R., Dickinson, D., Chen, P. and Chu, T.-C. (2013) Inhibition of Herpes Simplex Virus Type 1 with the Modified Green Tea Polyphenol Palmitoyl-Epigal- locatechin Gallate. Food and Chemical Toxicology, 52, 207-215.
http://dx.doi.org/10.1016/j.fct.2012.11.006

[20]   Fortin, H., Vigor, C., Lohezic-Dévéhat, F., Robin, V., Le Bossé, B., Boustie, J.M. and Amoros, M. (2002) In Vitro Antiviral Activity of Thirty-Six Plants from La Réunion Island. Fitoterapia, 73, 346-350.
http://dx.doi.org/10.1016/S0367-326X(02)00080-1

[21]   Andrighetti-Fröhner, C.R., Sincero, T.C., da Silva, A.C., Savi, L.A., Gaido, C.M., Bettega, J.M., Mancini, M., de Almeida, M.T., Barbosa, R.A., Farias, M.R., Barardi, C.R. and Simöes, C.M. (2005) Antiviral Evaluation of Plants from Brazilian Atlantic Tropical Forest. Fitoterapia, 76, 374-378.
http://dx.doi.org/10.1016/j.fitote.2005.03.010

[22]   Yarmolinsky, L., Hulelhel, M., Zaccai, M. and Ben-Shabat, S. (2012) Potent Antiviral Flavone Glycosides from Ficus benjamina Leaves. Fitoterapia, 83, 362-367.
http://dx.doi.org/10.1016/j.fitote.2011.11.014

[23]   Fioravanti, R., Celestino, I., Costi, R., Crucitti, G.C., Pescatori, L., Mattiello, L., Novellino, E., Checconi, P., Palamara, A.T., Nencioni, L. and Di Santo, R. (2012) Effects of Polyphenol Compounds on Influenza A Virus Replication and Definition of Their Mechanism of Action. Biorganic & Medicinal Chemistry, 20, 5046-5052.
http://dx.doi.org/10.1016/j.bmc.2012.05.062

[24]   Chiang, L.C., Chaing, W., Liu, M.C. and Lin, C.C. (2003) In Vitro Antiviral Activities of Caesalpinia pulcherrima and Its Related Flavonoids. Journal of Antimicrobial Chemotherapy, 52, 194-198.
http://dx.doi.org/10.1093/jac/dkg291

[25]   Hosseinzadeh, L., Behraven, J., Mosaffa, F., Bahrami, G., Bahrami, A. and Karimi, G. (2011) Curcumin Potentiates Doxorubicin-Induced Apoptosis in H9c2 Cardiac Muscle Cells through Generation of Reactive Oxygen Species. Food and Chemical Toxicology, 49, 1102-1109.
http://dx.doi.org/10.1016/j.fct.2011.01.021

[26]   Liang, G., Yang, S., Zhou, H. and Shao, L. (2009) Synthesis, Crystal Structure and Anti-Inflammatory Properties of Curcumin Analogues. European Journal of Medicinal Chemistry, 44, 915-919.
http://dx.doi.org/10.1016/j.ejmech.2008.01.031

[27]   Debata, R., Castellanos, M.R., Fata, J.E., Baggett, S., Rajupet, S., Szerszen, A., Begum, S., Mata, A., Murty, V.V., Opitz, L.M. and Banerjee, P. (2013) A Novel Curcumin-Based Vaginal Cream Vacurin Selectively Eliminates Apposed Human Cervical Cancer Cells. Gynecologic Oncology, 129, 145-153.
http://dx.doi.org/10.1016/j.ygyno.2012.12.005

[28]   Duvoix, A., Blasius, R., Delhalle, S., Schnekenburger, M., Morceau, F., Henry, E., Dicato, M. and Diederich, M. (2005) Chemopreventive and Therapeutic Effects of Curcumin. Cancer Letters, 223, 181-190.
http://dx.doi.org/10.1016/j.canlet.2004.09.041

[29]   Rechtman, M.M., Bar-Yishay, I., Fishman, S., Adamovich, Y., Shaul, Y., Halpern, Z. and Shlomai, A. (2010) Curcumin Inhibits Hepatitis B Virus Down Regulation of the Metabolic Coactivator PGC-1α. FEBS Letters, 584, 2485-2490.
http://dx.doi.org/10.1016/j.febslet.2010.04.067

[30]   Kim, H.J., Yoo, H.S., Kim, J.C., Park, C.S., Choi, M.S., Kim, M., Choi, H., Min, J.S., Kim, Y.S., Yoon, S.W. and Ahn, J.K. (2009) Antiviral Effect of Curcuma longa Linn Extract against Hepatitis B Virus Replication. Journal of Ethnopharmacology, 124, 189-196.
http://dx.doi.org/10.1016/j.jep.2009.04.046

[31]   Kim, K., Kim, K.H., Kim, H.Y., Cho, H.K., Sakamoto, N. and Cheong, J. (2010) Curcumin Inhibits Hepatitis C Virus Replication via Suppressing Aktk-SREBP-1 Pathway. FEBS Letters, 584, 707-712.
http://dx.doi.org/10.1016/j.febslet.2009.12.019

[32]   Anggakusuma, Colpitts, C.C., Schang, L.M., Rachmawati, H., Frentzen, A., Pfaender, S., Behrendt, P., Brown, R.J., Bankwitz, D., Steinmann, J., Ott, M., Meuleman, P. and Rice, C.M. (2013) Turmeric Curcumin Inhibits Entry of All Hepatitis C Virus Genotypes into Human Liver Cells. Gut, 63, 1137-1149.

[33]   Chen, D.-Y., Shien, J.-H., Tiley, L., Chiou, S.-S., Wang, S.-Y., Chang, T.-J., Lee, Y.-J., Chan, K.-W. and Hsu, W.-L. (2010) Curcumin Inhibits Influenza Virus Infection and Haemagglutination Activity. Food Chemistry, 119, 1346-1351.
http://dx.doi.org/10.1016/j.foodchem.2009.09.011

[34]   Qin, Y., Lin, X., Chen, Y., Wu, S., Si, X., Wu, H., Zhai, X., Wang, Y., Tong, L., Pan, B., Zhong, X., Wang, T., Zhao, W. and Zhang, Z. (2014) Curcumin Inhibits the Replication of Enterovirus 71 in Vitro. Acta Pharmaceutica Sinica B, 4, 284-294.
http://dx.doi.org/10.1016/j.apsb.2014.06.006

[35]   Marbawati, D. and Umniyati, S.R. (2015) Effects of Curcumin and Pentagamavunon-0 against Dengue-2 Virus Infection in Vero Cells; an in Vitro Study. Procedia Environmental Sciences, 23, 215-221.
http://dx.doi.org/10.1016/j.proenv.2015.01.033

[36]   Bourne, K.Z., Bourne, N., Reising, S.F. and Stanberry, L.R. (1999) Plant Products as Topical Microbicide Candidates: Assessment of in Vitro and in Vivo Activity against Herpes Simplex Virus Type 2. Antiviral Research, 42, 219-226.
http://dx.doi.org/10.1016/S0166-3542(99)00020-0

[37]   Kutluay, S.B., Doroghazi, J., Roemwe, M.E. and Triezenberg, S.J. (2008)Curcumin Inhibits Herpes Simplex Virus Immediate-Early Gene Expression by a Mechanism Independent of p300/CBP Histone Acetyltransferase Activity. Virology, 373, 239-247.
http://dx.doi.org/10.1016/j.virol.2007.11.028

[38]   Chiang, L.C., Chiang, W., Liu, M.C. and Lin, C.C. (2003) In Vitro Antiviral Activities of Caesalpinia pulcherrima and Its Related Flavonoids. Journal of Antimicrobial Chemotherapy, 52, 194-198.
http://dx.doi.org/10.1093/jac/dkg291

[39]   Brady, R. and Bernstein, D. (2004) Treatment of Herpes Simplex Infections. Antiviral Research, 61, 73-81.
http://dx.doi.org/10.1016/j.antiviral.2003.09.006

[40]   Fatahzadeh, M. and Schwartz, R.A. (2007) Human Herpes Simplex Virus Infections: Epidemiology, Pathogenesis, Symptomatology, Diagnosis, and Management. Journal of the American Academy of Dermatology, 57, 737-763.
http://dx.doi.org/10.1016/j.jaad.2007.06.027

[41]   Chen, T.Z., Chen, D.-Y., Wen, H.-W., Chiou, S.-S., Chen, J.-M., Wong, M.-L. and Hsu, W.-L. (2013) Inhibition of Enveloped Viruses Infectivity by Curcumin. PLoS ONE, 8, e62482.
http://dx.doi.org/10.1371/journal.pone.0062482

[42]   Lu, Y., Lei, N., Wang, D., An, Z., Li, G., Han, F., Liu, H. and Liu, L. (2014) Protective Effect of Curcumin against Cytomegalovirus Infection in Balb/c Mice. Environmental Toxicology and Pharmacology, 37, 1140-1147.
http://dx.doi.org/10.1016/j.etap.2014.04.017

[43]   Debata, P.R., Castellanos, M.R., Fata, J.E., Baggett, S., Rajupet, S., Szerszen, A., Begum, S., Mata, A., Murty, V.V., Opitz, L.M. and Banerjee, P. (2012) A Novel Curcumin-Based Vaginal Cream Vacurin Selectively Eliminates Apposed Human Cervical Cancer Cells. Gynecologic Oncology, 129, 145-153.
http://dx.doi.org/10.1016/j.ygyno.2012.12.005

 
 
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