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 JBiSE  Vol.9 No.3 , March 2016
13C Protein Oxidation in Breath: Is It Relevant for the Whole Body Protein Status?
Abstract: Introduction: The kinetics of protein oxidation, monitored in breath, and its contribution to the whole body protein status is not well established. Objectives: To analyze protein oxidation in various metabolic conditions we developed/validated a 13C-protein oxidation breath test using low enriched milk proteins. Method/Design: 30 g of naturally labeled 13C-milk proteins were consumed by young healthy volunteers. Breath samples were taken every 10 min and 13CO2 was measured by Isotope Ratio Mass Spectrometry. To calculate the amount of oxidized substrate we used: substrate dose, molecular weight and 13C enrichment of the substrate, number of carbon atoms in a substrate molecule, and estimated CO2-production of the subject based on body surface area. Results: We demonstrated that in 255 min 20% ± 3% (mean ± SD) of the milk protein was oxidized compared to 18% ± 1% of 30 g glucose. Postprandial kinetics of oxidation of whey (rapidly digestible protein) and casein (slowly digestible protein) derived from our breath test were comparable to literature data regarding the kinetics of appearance of amino acids in blood. Oxidation of milk proteins was faster than that of milk lipids (peak oxidation 120 and 290 minutes, respectively). After a 3-day protein restricted diet (~10 g of protein/day) a decrease of 31% ± 18% in milk protein oxidation was observed compared to a normal diet. Conclusions: Protein oxidation, which can be easily monitored in breath, is a significant factor in protein metabolism. With our technique we are able to characterize changes in overall protein oxidation under various meta-bolic conditions such as a protein restricted diet, which could be relevant for defining optimal protein intake under various conditions. Measuring protein oxidation in new-born might be relevant to establish its contribution to the protein status and its age-dependent development.
Cite this paper: Reckman, G. , Koehorst, M. , Priebe, M. , Schierbeek, H. and Vonk, R. (2016) 13C Protein Oxidation in Breath: Is It Relevant for the Whole Body Protein Status?. Journal of Biomedical Science and Engineering, 9, 160-169. doi: 10.4236/jbise.2016.93012.
References

[1]   Jackson, A.A. (1999) Limits of Adaptation to High Dietary Protein Intakes. European Journal of Clinical Nutrition, 53, S44-S52.
http://dx.doi.org/10.1038/sj.ejcn.1600743

[2]   Santesso, N., Akl, E.A., Bianchi, M., Mente, A., Mustafa, R., Heels-Ansdell, D. and Schünemann, H.J. (2012) Effects of Higher-versus Lower-Protein Diets on Health Outcomes: A Systematic Review and Meta-Analysis. European Journal of Clinical Nutrition, 66, 780-788.
http://dx.doi.org/10.1038/ejcn.2012.37

[3]   Pedersen, A.N., Kondrup, J. and Borsheim, E. (2013) Health Effects of Protein Intake in Healthy Adults: A Systematic Literature Review. Food & Nutrition Research, 57, 21245.
http://dx.doi.org/10.3402/fnr.v57i0.21245

[4]   Cuenca-Sánchez, M., Navas-Carrillo, D. and Orenes-Pinero, E. (2015) Controversies Surrounding High-Protein Intake: Satiating Effect and Kidney and Bone Health. Advances in Nutrition, 6, 260-266.
http://dx.doi.org/10.3945/an.114.007716

[5]   Durnin, J.V.G.A., Garlick, P., Jackson, A.A., Schürch, B., Shetty, P.S. and Waterlow, J.C. (1999) Report of the IDECG Working Group on Lower Limites of Energy and Protein and Upper Limits of Protein Intakes. European Journal of Clinical Nutrition, 53, S174-S176.
http://dx.doi.org/10.1038/sj.ejcn.1600758

[6]   Metges, C.C. and Barth, C.A. (2000) Metabolic Consequences of a High Dietary-Protein Intake in Adulthood: Assessment of the Available Evidence. Journal of Nutrition, 130, 886-889.

[7]   FAO (2013) Dietary Protein Quality Evaluation in Human Nutrition. FAO Food and Nutrition Paper 92.

[8]   Brands, B., Demmelmair, H. and Koletzko, B. (2014) How Growth Due to Infant Nutrition Influences Obesity and Later Disease Risk. Acta Pediatrica, 103, 578-585.
http://dx.doi.org/10.1111/apa.12593

[9]   Weber, M., Grote, V., Closa-Monsaterolo, R., Escribano, J., Langhendries, J., Dain, E., Giovannini, M., Verduci, E., Gruszfeld, D., Socha, P. and Koletzko, B. (2014) Lower Protein Content in Infant Formula Reduces BMI and Obesity Risk at School Age: Follow-Up of a Randomized Trial. American Journal of Clinical Nutrition, 99, 1041-1051.
http://dx.doi.org/10.3945/ajcn.113.064071

[10]   Grunewald, M., Hellmuth, C., Demmelmair, H. and Koletzko, B. (2015) Excessive Weight Gain during Full Breast- Feeding. Annals of Nutrition and Metabolism, 64, 271-275.
http://dx.doi.org/10.1159/000365033

[11]   Melnik, B.C. (2015) Milk—A Nutrient System of Mammalian Evolution Promoting mTORC1-Dependent Translation. International Journal of Molecular Sciences, 16, 17048-17087.
http://dx.doi.org/10.3390/ijms160817048

[12]   Smith, B.N. and Epstein, S. (1971) Two Categories of 13C/12C Ratios for Higher Plants. Plant Physiology, 47, 380-384.
http://dx.doi.org/10.1104/pp.47.3.380

[13]   Camin, F., Perini, M., Colombarie, G., Bontempo, L. and Versini, G. (2008) Influence of Dietary Composition on the Carbon, Nitrogen, Oxygen and Hydrogen Stable Isotope Ratios of Milk. Rapid Communications in Mass Spectrometry, 22, 1690-1696.
http://dx.doi.org/10.1002/rcm.3506

[14]   Haycock, G.B., Schwartz, G.J. and Wisotsky, D.H. (1978) Geometric Method for Measuring Body Surface Area: A Height-Weight Formula Validated in Infants, Children, and Adults. Journal of Pediatrics, 93, 62-66.
http://dx.doi.org/10.1016/S0022-3476(78)80601-5

[15]   Lefebvre, P., Mosora, F., Lacroix, M., Luyckx, A., Lopez-Habib, G. and Duchesne, J. (1975) Naturally Labeled 13C- Glucose Metabolic Studies in Human Diabetes and Obesity. Diabetes, 24, 185-189.
http://dx.doi.org/10.2337/diab.24.2.185

[16]   Evenepoel, P., Geypens, B., Luypaerts, A., Hiele, M., Ghoos, Y. and Rutgeerts, P. (1998) Digestibility of Cooked and Raw Egg Protein in Humans as Assessed by Stable Isotope Techniques. Journal of Nutrition, 128, 1716-1722.

[17]   Chleboun, J. and Kocna, P. (2005) Isotope Selective Nondispersive Infrared Spectrometry Can Compete with Isotope Ratio Mass Spectrometry in Cumulative 13CO2 Breath Tests: Assessment of Accuracy. Klinická Biochemie a Metabolismus, 13, 92-97.

[18]   Wagenmakers, A.J., Rehrer, N.J., Brouns, F., Saris, W.H. and Halliday, D. (1993) Breath 13CO2 Background Enrichment during Exercise: Diet-Related Differences between Europe and America. Journal of Applied Physiology, 74, 2353-2357.

[19]   Dubuc, M.C., Sébastien, H. and Brazier, J.L. (2000) 13C Basal Abundance of Expired CO2-Definition of Pre-Requisites for Kinetic Breath Tests. Isotopes in Environmental and Health Studies, 36, 177-188.
http://dx.doi.org/10.1080/10256010008032941

[20]   Tanis, A.A., van den Berg, J.W., Kroneman, R., Wattimena, J.L., Rietveld, T., Nieland, B.H. and Swart, G.R. (1998) Human Liver Glycogen Metabolism Assessed with a 13C-Enriched Diet and a 13CO2 Breath Test. European Journal of Clinical Investigation, 28, 466-474.
http://dx.doi.org/10.1046/j.1365-2362.1998.00316.x

[21]   Hirschl, A.M. and Makristathis, A. (2007) Methods to Detect Helicobacter pylori: From Culture to Molecular Biology. Helicobacter, 12, 6-11.
http://dx.doi.org/10.1111/j.1523-5378.2007.00560.x

[22]   McCue, M.D., Sivan, O., McWilliams, S.R. and Pinshow, B. (2009) Tracking the Oxidative Kinetics of Carbohydrates, Amino Acids and Fatty Acids in the House Sparrow Using Exhaled 13CO2. Journal of Experimental Biology, 213, 782- 789.
http://dx.doi.org/10.1242/jeb.039842

[23]   Perets, T.T., Shporn, E., Boltin, D., Dickman, R. and Niv, Y. (2015) Stability of 13C-Urea Breath Test Samples over Time in the Diagnosis of Helicobacter pylori. Journal of Clinical Laboratory Analysis.

[24]   Boirie, Y., Dangin, M., Gachon, P., Vasson, M., Maubois, J. and Beaufrère, B. (1997) Slow and Fast Dietary Proteins Differently Modulate Postprandial Protein Accretion. Physiology, 94, 14930-14935.
http://dx.doi.org/10.1073/pnas.94.26.14930

[25]   Wierdsma, N.J., Peters, J.H.C., van Bokhorst-de van der Schueren, M.A.E., Mulder, C.J.J., Metgod, I. and van Bodegraven, A.A. (2014) Bomb Calorimetry, the Gold Standard for Assessment of Intestinal Absorption Capacity: Normative Values in Healthy Ambulant Adults. Journal of Human Nutrition and Dietetics, 27, 57-64.
http://dx.doi.org/10.1111/jhn.12113

[26]   Kim, E., Coelho, D. and Blachier, F. (2013) Review of the Association between Meat Consumption and Risk of Colorectal Cancer. Nutrition Research, 33, 983-994.
http://dx.doi.org/10.1016/j.nutres.2013.07.018

[27]   Raguso, C.A., Pereira, P. and Young, V.R. (1999) A Tracer Investigation of Obligatory Oxidative Amino Acid Losses in Healthy, Young Adults. American Journal of Clinical Nutrition, 70, 474-483.

[28]   Evenepoel, P., Claus, D., Geypens, B., Maes, M., Hiele, M., Rutgeerts, P. and Ghoos, Y. (1998) Evidence for Impaired Assimilation and Increased Colonic Fermentation of Protein, Related to Gastric Acid Suppression Therapy. Alimentary Pharmacology & Therapeutics, 12, 1011-1019.
http://dx.doi.org/10.1046/j.1365-2036.1998.00377.x

[29]   Ghoos, Y. and Beaufrère, B. (1998) 13C Protein Breath Tests. Gut, 43, S23-S24.
http://dx.doi.org/10.1136/gut.43.2008.s23

[30]   Bammens, B., Evenepoel, P., Verbeke, K. and Vanrenterghem, Y. (2004) Impairment of Small Intestinal Protein Assimilation in Patients with End-Stage Renal Disease: Extending the Malnutrition-Inflammation-Atherosclerosis Concept. American Journal of Clinical Nutrition, 80, 1536-1543.

[31]   Bujko, J., Schreurs, V.V., Nolles, J.A., Verreijen, A.M., Koopmanschap, R.E. and Verstegen, M.W. (2007) Application of a [13CO2] Breath Test to Study Short-Term Amino Acid Catabolism during the Postprandial Phase of a Meal. British Journal of Nutrition, 97, 891-897.
http://dx.doi.org/10.1017/S0007114507433049

[32]   Nolles, J.A., Verreijen, A.M., Koopmanschap, R.E., Verstegen, M.W. and Schreurs, V.V. (2008) Postprandial Oxidative Losses of Free and Protein-Bound Amino Acids in the Diet: Interactions and Adaptation. Journal of Animal Physiology and Animal Nutrition, 93, 431-438.
http://dx.doi.org/10.1111/j.1439-0396.2008.00820.x

 
 
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