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 AJPS  Vol.7 No.3 , March 2016
Influence of BA and IBA or NAA Combinations on Micropropagation of Cryptolepis sanguinolenta
Abstract: The study being the first of its kind established an efficient protocol for micropropagation of Cryptolepis sanguinolenta, an important endangered medicinal plant species, used in the treatment of Malaria. For shoot induction, semi hard wood nodal segments were maintained on MS (Murashige and Skoog) nutrient medium supplemented with MS vitamins, 30 g/L sucrose, 3% gelrite and various auxin and cytokinin combinations. Treatments involved 6-benzyladenine (BA) at 0.5, 1.0, 1.5, 2.0, 2.5 or 3.0 mg/L in combination with 0.1 mg/L Indole 3-butyric acid (IBA) or Naphthaleneacetic acid (NAA). Control consisted of hormone free MS medium. BA and IBA combinations were found to be more efficient in shoot regeneration than the BA and NAA combinations. Cultures maintained on MS medium supplemented with 3 mg/L BA, in combination with 0.1 mg/L IBA recorded the highest shoot induction (100%), mean shoot length (1.28 cm) and mean number of nodes per explant (2.5). This, however, did not differ significantly from cultures maintained on 2 or 2.5 mg/L BA and 0.1 mg/L IBA supplemented MS medium. Regenerated shoots were transferred onto different media for root induction. Treatments consisted of full strength MS medium augmented with either 0.5 or 0.1 mg/L IBA, hormone free half strength MS medium and half strength MS medium augmented with 0.01 mg/L IBA. After six weeks of culture, no rooting was recorded in all treatments with the exception of half strength MS medium supplemented with 0.01 mg/L IBA, which recorded 60% rooting. Regenerated plantlets were successfully weaned and established in the greenhouse.
Cite this paper: Dwumawa Monney, M. , Amissah, N. and Blay, E. (2016) Influence of BA and IBA or NAA Combinations on Micropropagation of Cryptolepis sanguinolenta. American Journal of Plant Sciences, 7, 572-580. doi: 10.4236/ajps.2016.73050.
References

[1]   Kruidwis (2011) Kruidwis Descriptor.
https://sites.google.com/site/kruidwis/planten-van-a-tot-z/cryptolepis

[2]   Boye, G.L. (1989) Studies on Antimalarial Action of Cryptolepis sanguinolenta Extract. Proceedings of the International Symposium on East-West Medicine, Seoul, 10-11 October 1989, 243-251

[3]   Martin, K.P. (2002) Rapid Propagation of Holostemma adakodien Schult., a Rare Medicinal Plant, through Axillary Bud Multiplication and Indirect Organogenesis. Plant Cell Reports, 21, 112-117.
http://dx.doi.org/10.1007/s00299-002-0483-7

[4]   Chakradhar, T. and Pullaiah, T. (2006) Effect of Explant Source on Axillary Shoot Multiplication during Micropropagation of a Rare Medicinal Plant—Wakatta volubilis (L f). Stapf. Journal of Plant Biochemistry and Biotechnology, 15, 43-45.
http://dx.doi.org/10.1007/BF03321900

[5]   Faisal, M., Ahmad, N. and Anis, M. (2007) An Efficient Micropropagation System for Tylophora indica: An Endangered, Medicinally Important Plant. Plant Biotechnology Reports, 1, 155-161.

[6]   Dodds, J.H. and Roberts, L.W. (1995) Experiments in Plant Tissue Culture. Press Syndicate of the University of Cambridge, Melbourne.

[7]   Amissah, J.N., Spiller, M., Oppong, A., Osei-Safo, D., Owusu-Darko, R., Debener, T., Danquah, E.Y. and Addae-Mensah, I. (2016) Genetic Diversity and Cryptolepine Concentration of Cryptolepis sanguinolenta (Lindl). Schlt. from Selected Regions of Ghana. Journal of Applied Research on Medicinal and Aromatic Plants, 3, 34-41.
http://dx.doi.org/10.1016/j.jarmap.2015.12.005

[8]   Murashige, T. and Skoog, F. (1962) A Revised Medium for Rapid Bioassays with Tobacco Tissue Cultures. Physiologia Plantarum, 15, 473-497.
http://dx.doi.org/10.1111/j.1399-3054.1962.tb08052.x

[9]   Martin, K.P. (2003) Plant Regeneration through Somatic Embryogenesis on Holostemma ada-kodien, a Rare Medicinal Plant. Plant Cell Tissue and Organ Culture, 72, 79-82.
http://dx.doi.org/10.1023/A:1021229422172

[10]   Beena, M.R., Martin, K.P., Kirtil, P.B. and Hariharan, M. (2003) Rapid in Vitro Propagation of Medicinally Important Ceropegia candelabrum. Plant Cell, Tissue and Organ Culture, 72, 285-289.
http://dx.doi.org/10.1023/A:1022395809204

[11]   Arulanandam, L.P.J., Kumar, S.G. and Sawmini, M. (2011) Micropropagation and Conservation of Rare Medicinal Plant Wakatta volubilis (Linn.) Stapf. Indian Journal of Biotechnology, 10, 238-241.

[12]   Sudha, C.G., Krishnan, P.N. and Pushpangadan, P. (1998) In Vitro Propagation of Holostemma annulare (Roxb.) K. Schum., a Rare Medicinal Plant. In Vitro Cellular and Developmental Biology Plant, 34, 57-63.
http://dx.doi.org/10.1007/BF02823124

[13]   Vinothkumar, D., Murugavelh, S. and Senthilkumar, M. (2011) Clonal Propagation of Wakatta volubilis through Nodal Explants Culture. Ceylon Journal of Science (Biological Sciences), 40, 53-58.
http://dx.doi.org/10.4038/cjsbs.v40i1.3406

[14]   Nunes, E.C., Volkmer, C.C., Moreno, F.N. and Viana, A.M. (2002) In Vitro Culture of Cedrela fissilis Vellozo (Meliaceae). Plant Cell, Tissue and Organ Culture, 70, 259-268.
http://dx.doi.org/10.1023/A:1016509415222

[15]   Beegum, S.A., Martin, P.K., Zang, C., Nishita, J.K., Ligimol, Slater, A. and Madhusodanan, P.V. (2007) Organogenesis from Leaf and Internode Explants of Ophiorrhia prostrate, an Anticancer Drug (Camptothecin) Producing Plant. Electronic Journal of Biotechnology, 10, 115-123.
http://dx.doi.org/10.2225/vol10-issue1-fulltext-7

[16]   Jamaleddine, Z.O., Lyam, P., Fajimi, O., Giwa, A., Tina, A., Lawyer, E.F., Okere, A.U. and Odofin, W.T. (2011) In Vitro Growth Response of Artemisia annua Seeds to Different Concentrations of Plant Growth Regulators. African Journal of Biotechnology, 10, 17841-17844.
http://dx.doi.org/10.5897/ajb10.1289

[17]   Demeke, Y., Tefera, W., Dechassa, N. and Abebie, B. (2014) Effects of Plant Growth Regulators on in Vitro Cultured Nodal Explants of Cassava (Manihot esculenta Crantz) Clones. African Journal of Biotechnology, 13, 2830-2839.
http://dx.doi.org/10.5897/AJB2013.13287

[18]   Phyto Technology Laboratories (2011) Plant Growth Regulators.
http://www.phytotechlab.com

[19]   Sathyanarayana, B.N. and Varghese, D.B. (2007) Plant Tissue Culture; Practices and New Experimental Protocols. I. K. International Publishing Hse. Pvt. Ltd., New Delhi.

 
 
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