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 AJPS  Vol.7 No.3 , March 2016
Expression Analysis of Aldo-Keto Reductase 1 (AKR1) in Foxtail Millet (Setaria italica L.) Subjected to Abiotic Stresses
Abstract: Foxtail millet (Setaria italica L.) is a drought-tolerant millet crop of arid and semi-arid regions. Aldo-keto reductases (AKRs) are significant part of plant defence mechanism, having an ability to confer multiple stress tolerance. In this study, AKR1 gene expression was studied in roots and leaves of foxtail millet subjected to different regimes of PEG- and NaCl-stress for seven days. The quantitative Real-time PCR expression analysis in both root and leaves showed upregulation of AKR1 gene during PEG and salt stress. A close correlation exits between expression of AKR1 gene and the rate of lipid peroxidation along with the retardation of growth. Tissue-specific differences were found in the AKR1 gene expression to the stress intensities studied. The reduction in root and shoot growth under both stress conditions were dependent on stress severity. The level of lipid peroxidation as indicated by MDA formation was significantly increased in roots and leaves along with increased stress levels. Finally, these findings support the early responsive nature of AKR1 gene and seem to be associated at least in part with its ability to contribute in antioxidant defence related pathways which could provide a better protection against oxidative stress under stress conditions.
Cite this paper: Kirankumar, T. , Madhusudhan, K. , Nareshkumar, A. , Kiranmai, K. , Lokesh, U. , Venkatesh, B. and Sudhakar, C. (2016) Expression Analysis of Aldo-Keto Reductase 1 (AKR1) in Foxtail Millet (Setaria italica L.) Subjected to Abiotic Stresses. American Journal of Plant Sciences, 7, 500-509. doi: 10.4236/ajps.2016.73044.
References

[1]   Gill, S.S. and Tuteja, N. (2010) Reactive Oxygen Species and Antioxidant Machinery in Abiotic Stress Tolerance in Crop Plants. Plant Physiology and Biochemistry, 48, 909-930.
http://dx.doi.org/10.1016/j.plaphy.2010.08.016

[2]   Yamauchi, Y., Hasegawa, A., Taninaka, A., Mizutani, M. and Sugimoto, Y. (2011) NADPH-Dependent Reductases Involved in the Detoxificaiton of Reactive Carbonyls in Plants. The Journal of Biological Chemistry, 286, 6999-7009.
http://dx.doi.org/10.1074/jbc.M110.202226

[3]   Colrat, S., Latche, A., Guis, M., Pech, J.C., Bouzayen, M., Fallot, J. and Roustan, J.P. (1999) Purification and Characterization of a NADPH-Dependent Aldehyde Reductase from Mung Bean That Detoxifies Eutypine, a Toxin from Eutypa lata. Plant Physiology, 119, 621-626.
http://dx.doi.org/10.1104/pp.119.2.621

[4]   Turoczy, Z., Kis, P., Torok, K., Cserhati, M., Lendvai, A., Dudits, D. and Horvath, G.V. (2011) Overproduction of a Rice Aldo-Keto Reductase Increases Oxidative and Heat Stress Tolerance by Malondialdehyde and Methylglyoxal Detoxification. Plant Molecular Biology, 75, 399-412.
http://dx.doi.org/10.1007/s11103-011-9735-7

[5]   Hyndman, D., Bauman, D.R., Heredoa, V.V. and Penning, T.M. (2003) The Aldo-Keto Reductase Superfamily Homepage. Chemico-Biological Interactions, 143, 621-631.
http://dx.doi.org/10.1016/S0009-2797(02)00193-X

[6]   Bohren, K.M., Bullock, B., Wermuth, B. and Gabbay, K.M. (1989) The Aldo-Keto Reductase Superfamily: cDNAs and Deduced Amino Acid Sequences of Human Aldehyde and Aldose Reductase. The Journal of Biological Chemistry, 264, 9547-9551.

[7]   Mindnich, R.D. and Penning, T.M. (2009) Aldo-Keto Reductase (AKR) Superfamily: Genomics and Annotation. Genome Review, 3, 362-370.

[8]   Narawongsanont, R., Kabinpong, S., Auiyawong, B. and Tantitadapitak, C. (2012) Cloning and Characterization of AKR4C14, a Rice Aldo-Keto Reductase, from Thai Jasmine Rice. The Protein Journal, 31, 35-42.
http://dx.doi.org/10.1007/s10930-011-9371-8

[9]   Bartels, D., Engelhardt, K., Roncarati, R., Schneider, K., Rotter, M. and Salamini, F. (1991) An ABA and GA Modulated Gene Expressed in the Barley Embryo Encodes in Aldose Reductase Related Protein. The EMBO Journal, 5, 1037-1043.

[10]   Roncarati, R., Salamini, F. and Bartels, D. (1995) An Aldose Reductase Homologous Gene from Barley: Regulation and Function. The Plant Journal, 7, 809-822.
http://dx.doi.org/10.1046/j.1365-313X.1995.07050809.x

[11]   Oberschall, A., Deak, M., Torok, K., Sass, L., Vass, I., Kovacs, I., Feher, A., Dudits, D. and Horvath, G.V. (2000) A Novel Aldose/Aldehyde Reductase Protects Transgenic Plants against Lipid Peroxidation under Chemical and Drought Stress. The Plant Journal, 24, 437-446.
http://dx.doi.org/10.1046/j.1365-313x.2000.00885.x

[12]   Hegedus, A., Erdei, S., Janda, T., Toth, E., Horvath, G. and Dudits, D. (2004) Transgenic Tobacco Plants Overproducing Alfalfa Aldose/Aldehyde Reductase Show Higher Tolerance to Low Temperature and Cadmium Stress. Plant Science, 166, 1329-1333.
http://dx.doi.org/10.1016/j.plantsci.2004.01.013

[13]   Hideg, E., Nagy, T., Oberschall, A., Dudits, D. and Vass, I. (2003) Detoxification Function of Aldose/Aldehyde Reductase during Drought and Ultra Violet-B (230-320 nm) Stresses. Plant, Cell and Environment, 26, 513-522.
http://dx.doi.org/10.1046/j.1365-3040.2003.00982.x

[14]   Eva, C., Zelenyanszki, H., Farkas, R.T. and Tamas, L. (2014) Transgenic Barley Expressing the Arabidopsis AKR4C9 Aldo-Keto Reductase Enzyme Exhibits Enhanced Freezing Tolerance and Regenerative Capacity. South African Journal of Botany, 93, 179-184.
http://dx.doi.org/10.1016/j.sajb.2014.04.010

[15]   Kanayama, Y., Mizutani, R., Yaguchi, S., Hojo, A., Ikeda, H., Nishiyama, M. and Kanahama, K. (2014) Characterization of an Uncharacterized Aldo-Keto Reductase Gene from Peach and Its Role in Abiotic Stress Tolerance. Phytochemistry, 104, 30-36.
http://dx.doi.org/10.1016/j.phytochem.2014.04.008

[16]   Simpson, P.J., Tantitadapitak, C., Reed, A.M., Mather, O.C., Bunce, C.M., White, S.A. and Ride, J.P. (2009) Characterization of Two Novel Aldo-Keto Reductases from Arabidopsis: Expression Patterns, Broad Substrate Specificity, and an Open Active-Site Structure Suggest a Role in Toxicant Metabolism Following Stress. Journal of Molecular Biology, 392, 465-480.
http://dx.doi.org/10.1016/j.jmb.2009.07.023

[17]   Hur, Y., Shin, K., Kim, S., Nam, K.H., Lee, M., Chun, J. and Cheon, C. (2009) Overexpression of GmAKR1 a Stress-Induced Aldo-Keto Reductase from Soybean, Retards Nodule Development. Molecules and Cells, 27, 217-223.
http://dx.doi.org/10.1007/s10059-009-0027-x

[18]   Bailly, C., Benamar, A. and Corbineau, Y. (1996) Changes in Malondialdehyde Content and in Superoxide Dismutase, Catalase and Glutathione Reductase Activities in Sunflower Seeds as Related to Deterioration during Accelerated Aging. Physiolagia Plantarum, 97, 104-110.
http://dx.doi.org/10.1111/j.1399-3054.1996.tb00485.x

[19]   Yi, F., Xie, S., Liu, Y., Qi, X. and Yu, J. (2013) Genome-Wide Characterization of microRNA in Foxtail Millet (Setaria italica). BMC Plant Biology, 13, 212-226.

[20]   Veeranagamalllaiah, G., Ranganayakulu, G.S., Thippeswamy, M., Sivakumar, M., Eswaranarayana Reddy, K., Pandurangaiah, M., Sridevi, V. and Sudhakar, C. (2009) Aldose Reductase Expression Contributes in Sorbital Accumulation and 4-Hydroxynon-2-enal Detoxification in Two Foxtail Millet (Setaria italica L.) Cultivars with Different Salt Stress Tol-erance. Plant Growth Regulation, 59, 137-143.
http://dx.doi.org/10.1007/s10725-009-9396-6

[21]   Chen, Z., Chen, M., Xu, Z.S., Li, L.C., Chen, X.P. and Ma, Y.Z. (2014) Characteristics and Expression Patterns of the Aldehyde Dehydrogenase (ALDH) Gene Superfamily of Foxtail Millet (Setaria italica L.). PLOS ONE, 9, 7.
http://dx.doi.org/10.1371/journal.pone.0101136

[22]   Heath, R.L. and Packer, L. (1968) Photoperoxidation in Isolated Chloroplasts: I. Kinetics and Stoichiometry of Fatty acid Peroxidation. Archives in Biochemistry and Biophysics, 125, 189-198.
http://dx.doi.org/10.1016/0003-9861(68)90654-1

[23]   Dekkers, B.J.W., Willems, L., Bassel, G.W., Marieke, R.P., Veldkamp, V.B., Ligterink, W., Hilhorst, H.W.M. and Bentsink, L. (2012) Identification of Reference Genes for RT-qPCR Expression Analysis in Arabidopsis and Tomato Seeds. Plant Cell Physiology, 53, 28-37.
http://dx.doi.org/10.1093/pcp/pcr113

[24]   Caldana, C., Scheible, W.R., Roeber, B.M. and Ruzicic, S. (2007) A Quantitative RT-PCR Platform for High-Through-put Expression Profiling of 2500 Rice Transcription Factors. Plant Methods, 3, 1.
http://dx.doi.org/10.1186/1746-4811-3-7

[25]   Veeranagamallaiah, G., Chandraobulreddy, P., Jyothsnakumari, G. and Sudhakar, C. (2007) Glutamine Synthetase Expression and Pyrroline-5-Carboxylate Reductase Activity Influence Proline Accumulation in Two Cultivars of Foxtail Millet (Setaria italica L.) with Differential Salt Sensitivity. Environmental and Experimental Botany, 60, 239-244.
http://dx.doi.org/10.1016/j.envexpbot.2006.10.012

[26]   Kusvuran, S., Ellioltioglu, S. and Polat, Z. (2013) Antioxidative Enzyme Activity, Lipid Peroxidation, and Proline Accumulation in the Callus Tissues of Salt and Drought Tolerant and Sensitive Pumpkin Genotypes under Chilling Stress. Horticulture, Environment and Biotechnology, 54, 319-325.
http://dx.doi.org/10.1007/s13580-013-1042-6

[27]   Lata, C. (2015) Advances in Omics for Enhancing Abiotic Stress Tolerance in Millets. Proceedings of Indian National Science Academy, 81, 397-415.

[28]   Hernandez, J.A. and Almansa, M.S. (2002) Short-Term Effects of Salt Stress on Antioxidant Systems and Leaf Water Relations of Pea Leaves. Physiologia Plantarum, 115, 251-257.
http://dx.doi.org/10.1034/j.1399-3054.2002.1150211.x

[29]   Sairam, R.K. and Tyagi, A. (2004) Physiology and Molecular Biology of Salinity Stress Tolerance in Plants. Current Science, 86, 407-421.

[30]   Wang, X. and Hun, J. (2009) Changes of Proline Content Antioxidation Isoforms in Two Alfalfa Cultivars under Salt Stress. Agricultural Sciences in China, 8, 431-440.
http://dx.doi.org/10.1016/S1671-2927(08)60229-1

[31]   Suzuki, N. and Mittler, R. (2006) Reactive Oxygen Species and Temperature Stresses: A Delicate Balance between Signaling and Destruction. Physiologia Plantarum, 126, 46-51.
http://dx.doi.org/10.1111/j.0031-9317.2005.00582.x

[32]   Yamunchi, Y., Hasegawa, A., Mizutani, M. and Sugimoto, Y. (2012) Chloroplastic NADPH-Dependent Alkenal/One Oxidoreductase Contributes to the Detoxification of Reactive Carbonyls Produced under Oxidative Stress. Federation of European Biochemical Societies Letters, 586, 1208-1213.
http://dx.doi.org/10.1016/j.febslet.2012.03.013

[33]   Reddy, D.Y., Reddy V.R. and Anbumozhi, V. (2003) Physiological Responses of Groundnut (Arachis hypogea L.) to Drought Stress and Its Amelioration: A Critical Review. Plant Growth Regulation, 41, 75-88.
http://dx.doi.org/10.1023/A:1027353430164

[34]   Bowler, C., Montagu, M.V. and Inze, D. (1992) Superoxide Dismutase and Stress Tolerance. Annual Review of Plant Physiology and Molecular Biology, 43, 83-116.
http://dx.doi.org/10.1146/annurev.pp.43.060192.000503

[35]   Sree, B.K., Rajendrakumar, S.V. and Reddy, A.R. (2000) Aldose Reductase in Rice (Oryza sativa L.): Stress Response and Developmental Specificity. Plant Science, 160, 149-157.
http://dx.doi.org/10.1016/S0168-9452(00)00376-9

 
 
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