t selective pressure from the use of anti-microbial agents is a major determinant for the emergence of resistant strains  -  that could be considerable. Our results indicated that the rate of ciprofloxacin resistance pattern against P. aeruginosa was found to be relatively high percent in our population. Other studies show that ciprofloxacin resistance pattern was 23% in Spain  , 31.9% in Italy  and 26.8% in Latin America  . Overall results show that the rate of P. aeruginosa antibiotic resistance was observed in highest rate in case of Trimetoprime Solfametaxasole, while, Ciprofloxacin, Imipenem and Amikacin were identified as the most effective antibiotics in this study against P. aeruginosa. In previous studies it was reported that resistance to Imipenem was 14% in Spain  , 19.3% in Italy  and 68% in Saudi Arabia  that approved with our results. Previous studies show that antibacterial effects of Amikacin against pseudomonal were greater than Gentamycin   and only 0.9% resistant reported to Amikacin in Turkey  . So, Amikacin is effective antibiotic against P. aeruginosa in their study. In current study, resistance pattern to Piperacillin was also higher, while Piperacillin resistance rate was 10% in Spain  , 12% in Italy  , 14% in Latin American population  . Indeed P. aeruginosa strains Infections, most be treated as soon as possible due to their severe resistance against antibiotics. We have to apply an accurate antibiotic treatment discipline, according to the findings based on antibiogram method in order to prevent its spread. Data showed that this bacterium has developed its resistance pattern against amikacin and Ciprofloxacin in comparison to previous years. So the use of effective infection control practices can go a long way to limiting the development and spread of antimicrobial resistance ensuring that these agents continue to find a place in treatment of P. aeruginosa infections and also, hospital infection monitoring committee needs to force the tasks of more severe monitoring on hospital pathogens and bacterial resistance pattern development studies as important objectives to finding effective antibiotics against P. aeruginosa.
 Ryan, K.J. and Ray, C.G. (Eds.) (2004) Sherris Medical Microbiology. 4th Edition, McGraw Hill, New York.
 Snell, K., Holderi, A., Lepplas, A. and Saelinger, C.B. (1978) Role of Exotoxin and Protease as Possible Virulence Factors in Experimental Infections with Pseudomonas aeruginosa. Journal of Clinical Microbiology, 9, 538-540.
 Echere, J.C. and Kohler, T. (1999) Patterns and Modes of β-Lactam Resistance in Pseudomonas aeruginosa. Clinical Microbiology and Infection, 5, S15-S18. http://dx.doi.org/10.1111/j.1469-0691.1999.tb00719.x
 Khan, J.A., Iqbal, Z., Rahman, S.U., Farzana, K. and Khan, A. (2008) Prevalence and Resistance Pattern of Pseudomonas aeruginosa against Various Antibiotics. Pakistan Journal of Pharmaceutical Sciences, 21, 311-315.
 Holder, I.A., Nathan, P. and Macmillan, B.G. (1973) Burn Wounds: Microbiology, Local Host Defences, and Current Therapy. Critical Reviews in Clinical Laboratory Science, 4, 61-100. http://dx.doi.org/10.3109/10408367309151684
 Erdem, B. (1999) Pseudomonas. In: Ustacelebi, S., Ed., Basic ClinMicrobiol., Gunes Publication, Ankara, 551-558.
 Govan, J.R.W. (1992) Microbiology of Lung Infection in Cystic Fibrosis. British Medical Bulletin, 48, 912-930.
 Levinson, W. and Jawetz, E. (2000) Medical Microbiology and Immunology. 6th Edition, McGraw-Hill, New York, p. 123.
 Dark, J.K.G. (1988) Pathogenesis and Therapy of Pseudomonas aeruginosa. Eye, 2, 46-55. http://dx.doi.org/10.1038/eye.1988.133
 Smith, J., Payne, J. and Berne, T. (2000) The Surgeon’s Guide to Antimicrobial Chemotherapy. CRC Press, Boca Raton, 38-74.
 O’Donnell, J.G., Sorbello, A.F., Condoluci, D.V. and Barnish, M.J. (1993) Sinusitis Due to Pseudomonas aeruginosa in Patients with Human Immunodeficiency Virus Infection. Clinical Infectious Diseases, 16, 404-406. http://dx.doi.org/10.1093/clind/16.3.404
 Flores, G., Stavola, J.J. and Noel, G.J. (1993) Bacteremia Due to Pseudomonas aeruginosa in Children with AIDS. Clinical Infectious Diseases, 16, 706-708. http://dx.doi.org/10.1093/clind/16.5.706
 Itah, A.Y. and Essien, J.P. (2005) Growth Profile and Hydrocarbonoclastic Potential of Microorganisms Isolated from Tarballs in the Bight of Bonny, Nigeria. World Journal of Microbiology and Biotechnology, 21, 1317-1322. http://dx.doi.org/10.1007/s11274-004-6694-z
 Adedeji, G.B., Fagade, O.E. and Oyelade, A.A. (2007) Prevalence of Pseudomonas aeruginosa in Clinical Samples and Its Sensitivity to Citrus Extract. African Journal of Biomedical Research, 10, 183-187.
 Carmeli, Y., Troillet, N., Eliopoulos, G.M. and Samore, M.H. (1999) Emergence of Antibiotic-Resistant Pseudomonas aeruginosa: Comparison of Risks Associated with Different Anti Pseudomonal Agents. Antimicrobial Agents and Chemotherapy, 43, 1379-1382.
 Linden, P.K., Kusne, S., Coley, K., Fontes, P., Kramer, D.J. and Paterson, D. (2003) Use of Parenteral Colistin for the Treatment of Serious Infection Due to Antimicrobial-Resistant Pseudomonas aeruginosa. Clinical Infectious Diseases, 37, e154-e160. http://dx.doi.org/10.1086/379611
 Todar, K. (2002) Todar’s Online Textbook of Bacteriology. pp. 1-8. http://textbookofbacteriology.net/nutgro.html
 Anab, F., Syed Baqir, N., Sheikh, A., Shaheen, P. and Sabahat, J. (2012) Antimicrobial Susceptibility Pattern of Clinical Isolates of Pseudomonas aeruginosa Isolated from Patients of Lower Respiratory Tract Infections. SpringerPlus, 1, 70.
 das Neves, M.T., de Lorenzo, M.E., Almeida, R.A. and Fotaleza, C.M. (2010) Antimicrobial Use and Incidence of Multidrug-Resistant Pseudomonas aeruginosa in a Teaching Hospital: An Ecological Approach. Revista da Sociedade Brasileira de Medicina Tropical, 43, 629-632. http://dx.doi.org/10.1590/S0037-86822010000600005
 Arshi, S., Manzoor, T., Syed, S. and Assadullah, S. (2007) In Vitro Sensitivity Pattern of Pseudomonas aeruginosa Strain Isolated from Patient Skims—Role of Antimicrobial in the Emergence of Multiple Resistance Strains. JK-Practitioner, 14, 31-34.
 Murase, M., Miyamoto, H., Handa, T., Sahaki, S. and Takenchi, N. (1995) Activities of Anti-Pseudomonal Agent against Clinical Isolates of Pseudomonas aeruginosa. The Japanese Journal of Antibiotics, 48, 1581-1589.
 Van Landuyt, H.W., Boelaert, J., Glibert, B., Gordts, B. and Verbruggen, A.M. (1986) Surveillance of Aminoglycoside Resistance. European Data. The American Journal of Medicine, 80, 76-81. http://dx.doi.org/10.1016/0002-9343(86)90483-3
 Alkalin, H.E., Torun, M. and Alacam, R. (1988) Aminoglycoside Resistance Patterns in Turkey. Scandinavian Journal of Infectious Diseases, 20, 199-203. http://dx.doi.org/10.3109/00365548809032438
 Jarvis, W.R. (1996) Preventing the Emergence of Multidrug Resistant Microorganisms through Antimicrobial Use Controls: The Complexity of the Problem. Infection Control and Hospital Epidemiology, 17, 490-495. http://dx.doi.org/10.2307/30141280
 Weber, D.J., Raasch, R. and Rutala, W.A. (1999) Nosocomial Infections in the ICU: The Growing Importance of Antibiotic Resistant Pathogens. Chest, 115, 34S-41S. http://dx.doi.org/10.1378/chest.115.suppl_1.34S
 Nathwani, D. (1998) Sequential Switch Therapy for Lower Respiratory Tract Infections: A European Perspective. Chest, 113, 211S-218S. http://dx.doi.org/10.1378/chest.113.3_Supplement.211S
 Bouza, E., Garcia-Gorrote, F., Cercenado, E., Marin, M. and Diaz, M.S. (1999) Pseudomonas aeruginosa: A Survey of Resistance in 136 Hospitals in Spain. The Spanish Pseudomonas aeruginosa Study Group. Antimicrobial Agents and Chemotherapy, 43, 981-982.
 Bonfiglio, G., Carciotto, V., Russo, G., Stefani, S., Schito, G.C., Debbia, E. and Nicoletti, G. (1998) Antibiotic Resistance in Pseudomonas aeruginosa: An Italian Survey. Journal of Antimicrobial Chemotherapy, 41, 307-310. http://dx.doi.org/10.1093/jac/41.2.307
 Jones, R.N. (2001) Resistance Patterns among Nosocomial Pathogens: Trends over the Past Few Years. Chest, 119, 397S-404S. http://dx.doi.org/10.1378/chest.119.2_suppl.397S
 Rotimi, V.O., Al-Sweih, N.A. and Feteih, J. (1998) The Prevalence and Antibiotic Susceptibility Pattern of Gram-Negative Bacterial Isolates in Two ICUs in Saudi Arabia and Kuwait. Diagnostic Microbiology and Infectious Disease, 30, 53-59. http://dx.doi.org/10.1016/S0732-8893(97)00180-6
 Maes, P. and Vanhoof, R. (1992) A 56-Months Prospective Surveillance Study on the Epidemiology of Aminoglycoside Resistance in a Belgian General Hospital. Scandinavian Journal of Infectious Diseases, 24, 495-501. http://dx.doi.org/10.3109/00365549209052636